BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4253.p (249 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 80 2e-16 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 68 9e-13 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 64 1e-11 gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum s... 63 2e-11 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 62 4e-11 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 74/238 (31%), Positives = 103/238 (43%), Gaps = 14/238 (5%) Query: 7 LVVGGSKGTGLALVEHLLADGQRVSVLARSACALPPSPL-----LTWLPVDVTDTVALHT 61 +V GGS+G GLA + A+G RV V R A L + +P D L Sbjct: 13 VVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPGDAAKLDDLDR 72 Query: 62 ALDACVAGQGKIDSAVFMQRHRASDDAF------ETDLAVALTATRATIDHLVARKHFSG 115 A G++D +F AS +A E D + V +K Sbjct: 73 LYARVQADHGRLD-VLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFSV-QKALPL 130 Query: 116 SAQGNGIVMVSSIADHYIAPEQDLGYHVSKAGLVQLARFYALQLGPLGLRVNVVSPCVVI 175 +QG IV+ S+A P Q L Y+ SKA + AR + L G+RVNVV+P Sbjct: 131 MSQGGAIVLNGSVAGVKGFPGQSL-YNASKAAVRSFARSWTADLKERGIRVNVVAPGGTE 189 Query: 176 KEAAQEYYRQNSWITDRFEHFIPLRRAGSSQDIINAILFLTGAQSSYITGQNIVVDGG 233 ++Y + D + +PL R G +I A+LFL A+SSYI G + VDGG Sbjct: 190 TRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAGVELFVDGG 247 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 67.8 bits (164), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 73/236 (30%), Positives = 103/236 (43%), Gaps = 14/236 (5%) Query: 7 LVVGGSKGTGLALVEHLLADGQRVSVLARSA---CALPPSPLLTWLPVDVTDTVALHTAL 63 ++ G +G G A L G +V LAR+A L + VD++D A A+ Sbjct: 9 IITGAGRGIGRACALLLAQRGAQVIALARTASDLARLQEEIGARVIAVDLSDPAAARRAM 68 Query: 64 DACVAGQGKIDSA---VFMQRHRASDDAFETDLAVALTATRATIDHLVARKHFSGSAQGN 120 I+SA V SD+ +E L V L A T AR AQG Sbjct: 69 AEAGTADFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAF-ARMRI---AQGG 124 Query: 121 G--IVMVSSIADHYIAPEQDLGYHVSKAGLVQLARFYALQLGPLGLRVNVVSPCVVIKEA 178 G +V ++SIA H EQ L Y SKAGL R A +LGP G+RVN V+P + + E Sbjct: 125 GGAMVNITSIAGHR-GFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTEL 183 Query: 179 AQEYYRQNSWITDRFEHFIPLRRAGSSQDIINAILFLTGAQSSYITGQNIVVDGGL 234 A+ + + P R S +D+ +I L ++ + G + VDGG Sbjct: 184 AEAAWSDPAKSGPMMARH-PSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGF 238 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 63.9 bits (154), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 67/238 (28%), Positives = 106/238 (44%), Gaps = 25/238 (10%) Query: 7 LVVGGSKGTGLALVEHLLADGQRVSVLARSACALPPSPLLTWLPVDVT---DTVALHTAL 63 LV GG++G G A+ L+A G RV A LPP + +VT VA ++ Sbjct: 14 LVTGGTQGIGAAVARQLVALGARVI-----AAGLPPFAHEEFAGSEVTLVEMNVASEESV 68 Query: 64 DACVAGQGKID-----SAVFMQRHRASDDAFETDLAVALTATRATIDHLVARKHFSGSAQ 118 A +AG ++D + + + + FE +AV LT T AR+ Q Sbjct: 69 RAALAGLTQLDIVVNCAGIIKRGAELETEVFEQVIAVNLTGTMRVCAA--ARELLR---Q 123 Query: 119 GNGIVMVSSIADHYIAPEQDLGYHVSKAGLVQLARFYALQLGPLGLRVNVVSPCVV---I 175 G ++ ++ + GY SK G+ QL + A+ G+RVN V+P + + Sbjct: 124 SRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPL 183 Query: 176 KEAAQEYYRQNSWITDRFEHFIPLRRAGSSQDIINAILFLTGAQSSYITGQNIVVDGG 233 +A Q+ + I R PL R G +D+ + FL +S++TG + VDGG Sbjct: 184 TQALQDDPVRAGPILAR----TPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGG 237 >gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 63.2 bits (152), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 70/264 (26%), Positives = 111/264 (42%), Gaps = 50/264 (18%) Query: 7 LVVGGSKGTGLALVEHLLADGQRVSVLARS-------ACALPPSPLLTWLPV--DVTDTV 57 +V GGS G G A+ E L +G RV+++ARS A AL S + V DV++T Sbjct: 11 VVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVIAVAADVSNTE 70 Query: 58 ALHTALDACVAGQGKIDSAVFMQRH----------RASDDAFETDLAVALTA----TRAT 103 + A+ G+ID V H AS + D+ + + +A Sbjct: 71 QVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASPEGLLEDINIKVVGYMRFAKAV 130 Query: 104 IDHLVA----RKHFSGSAQGNGIVMVSSIADHYIAPEQDLGYHVSKAGLVQLARFYALQL 159 H+ A R G G G +S + + + L + + QL Sbjct: 131 APHMRAGGYGRIINIGGLTGRGSKQLSGMRN---------------VAICHLTKTLSDQL 175 Query: 160 GPLGLRVNVVSPCVVIKEAAQEYYRQNS--------WITDRFEHFIPLRRAGSSQDIINA 211 GP G+ VNV+ P VV E Y + + + + P+RR ++I + Sbjct: 176 GPDGITVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADV 235 Query: 212 ILFLTGAQSSYITGQNIVVDGGLT 235 +LFL +++ ITG++I VDGG+T Sbjct: 236 VLFLASPRAAAITGESIAVDGGIT 259 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 62.4 bits (150), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 32/94 (34%), Positives = 53/94 (56%), Gaps = 2/94 (2%) Query: 141 YHVSKAGLVQLARFYALQLGPLGLRVNVVSPCVVIKEAAQEYYRQNSWITDRFEHFIPLR 200 Y SKA ++ L R A++L G+ VN V+P V Q + + + + + +P+R Sbjct: 157 YGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPVDTPMTQAMHSEQT--RESYYRLVPMR 214 Query: 201 RAGSSQDIINAILFLTGAQSSYITGQNIVVDGGL 234 R G++ +I +A+ +L +S+YITG I VDGG Sbjct: 215 RYGTTDEIASAVCYLAAEESAYITGHVIPVDGGF 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 785,340 Number of extensions: 31443 Number of successful extensions: 195 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 18 Length of query: 249 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 166 Effective length of database: 1,226,865 Effective search space: 203659590 Effective search space used: 203659590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)