BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4249.p (351 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 176 2e-45 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 144 1e-35 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 119 5e-28 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 79 6e-16 gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 70 3e-13 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 176 bits (446), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 124/352 (35%), Positives = 180/352 (51%), Gaps = 32/352 (9%) Query: 15 LQQPLIVDEVELPPQLEVGQVLVKIGVSGVCGSQLGEIDGAKGEDKYLPHLLGHEASGIV 74 + QPL ++E++L P G+VLV++ +G+C S L I G + + +P LGHEAS V Sbjct: 19 VSQPLKIEELDLDPP-GPGEVLVRMKAAGLCHSDLSVITGVR--PRPVPMALGHEASAEV 75 Query: 75 QEVGPGVRFVKPGDHVVLHWRKSQGIESLP-----PKYTWRGKTLN------AGL----- 118 EVG GV ++ GD VVL + S G LP P G N +G Sbjct: 76 VEVGSGVNDLRAGDLVVLVFVPSCG-HCLPCMEGRPALCEPGAATNTVGELLSGQRRLRD 134 Query: 119 ----------IATFNEYAIVSENRVTRIDPSSDMDVAALFGCAVTTGFGVAENNADIRLG 168 ++ F ++A+VS +I+ D AALFGCAV TG G A N A+++ G Sbjct: 135 KTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAEVKAG 194 Query: 169 ESVVVFGAGGVGLNIIQAASLRSAYPIIAVDLHEGRLELAKQVGATHLINASKVDARTAL 228 + V G GGVGL + A A +IAVDLH+ +L LA+ +GAT +NA DA Sbjct: 195 TTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDA-VEK 253 Query: 229 VELMGKQGIDVFIDNTGQPPIIEMGYELTKAQGRVVLVGVPRAGRNISIYSLPL-HFEKT 287 V + + G+D + G +E Y +T+ G V G+P G++ ++ + L E+T Sbjct: 254 VRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVAEERT 313 Query: 288 ISGSHGGETVPHVDIPRYTKLLDAGLIQLKPLITEHFKLDDINVAIERMRSG 339 + GS+ G VP DIPRY L AG + + L+ E L DIN +R+ G Sbjct: 314 VKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFDRLHGG 365 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 144 bits (363), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 115/358 (32%), Positives = 172/358 (48%), Gaps = 28/358 (7%) Query: 8 RAAILAQLQQPLIVDEVELPPQLEVGQVLVKIGVSGVCGSQLGEIDGAKGEDKYLPHLLG 67 RAA+ Q +PL ++EVEL + G+VLV+I +G+C + + GA E + P +LG Sbjct: 4 RAAVAWQAGKPLTIEEVELGGP-KAGEVLVEIKATGICHTDYYTLSGADPEGIF-PAILG 61 Query: 68 HEASGIVQEVGPGVRFVKPGDHVVLHW---------------------RKSQGIESLP-- 104 HE +G+V +VGPGV+ +K DHV+ + R +QG +P Sbjct: 62 HEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDA 121 Query: 105 -PKYTWRGKTLNAGL-IATFNEYAIVSENRVTRIDPSSDMDVAALFGCAVTTGFGVAENN 162 +++ GK L + +TF+ Y +V E + +I + D GC VTTG G Sbjct: 122 TSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVTTGVGAVLFT 181 Query: 163 ADIRLGESVVVFGAGGVGLNIIQAASLRSAYPIIAVDLHEGRLELAKQVGATHLINASKV 222 A + G +VVVFG GG+GLN+IQAA + A II VDL+ R +A++ G T IN V Sbjct: 182 AKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKDV 241 Query: 223 DARTALVELMGKQGIDVFIDNTGQPPIIEMGYELT-KAQGRVVLVGVPRAGRNISIYSLP 281 + M G D + G + E K G+ +++GV AG+ IS Sbjct: 242 PNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPFQ 301 Query: 282 LHFEKTISGSHGGETVPHVDIPRYTKLLDAGLIQLKPLITEHFKLDDINVAIERMRSG 339 L + GS G D+P+ G + + LIT L+ IN + M+SG Sbjct: 302 LVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSG 359 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 119 bits (297), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 101/354 (28%), Positives = 167/354 (47%), Gaps = 25/354 (7%) Query: 5 KTMRAAILAQLQQPLIVDEVELPPQLEVGQVLVKIGVSGVCGSQLGEIDGAKGEDKYLPH 64 + M+AA++ + +PL +++V +P GQ+LVK SGVC + L G P Sbjct: 3 QMMKAAVVREFGKPLSIEQVPVPTPAP-GQILVKFEASGVCHTDLHAAHGDWPVKPTPPF 61 Query: 65 LLGHEASGIVQEVGPGVRFVKPGDHVVLHWRKSQGIESLPPKYTWR---GKTLNAG--LI 119 + GHE +G V VG GV+ VK GD V + W + P + W + N G + Sbjct: 62 IPGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSVN 121 Query: 120 ATFNEYAIVSENRVTRIDPSSDMDVAALFGCA-VTTGFGVAENNADIRLGESVVVFGAGG 178 +F EY + V + + D AA CA VT G+ E ++R GE VV+ G GG Sbjct: 122 GSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKET--EVRPGEWVVISGIGG 179 Query: 179 VGLNIIQAASLRSAYPIIAVDLHEGRLELAKQVGATHLINASKVDARTALVELMGKQGID 238 +G +Q A + ++A D+HE +L LAK++GA ++ +A + ++G G Sbjct: 180 LGHMAVQYAKAMGMH-VVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIG--GAH 236 Query: 239 VFIDNTGQPPIIEMGYELTKAQGRVVLVGVPRAGRNISIYSLPLHFEKTISGSHGGETVP 298 + P +E + +A+G + LVG+P ++ +++ L T+ GS G Sbjct: 237 GALVTAVSPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLK-RITVRGSIVGTR-- 293 Query: 299 HVDIPRYTKLLDAGLIQLKPLITEHF---KLDDINVAIERMRSGAMSGRCLIRM 349 L ++ + + + HF KLD+IN RM G + GR ++ + Sbjct: 294 -------QDLEESLVFAAEGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLDL 340 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 79.0 bits (193), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 97/369 (26%), Positives = 153/369 (41%), Gaps = 57/369 (15%) Query: 10 AILAQLQQPLIVDEVELPPQLEVGQVLVKIGVSGVCGSQL----GEIDGAKGEDKYLPHL 65 A+L + V+ V P E V++K+ + +CGS L G+I K D + Sbjct: 3 ALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGKIPATKAGD-----I 57 Query: 66 LGHEASGIVQEVGPGVRFVKPGDHVVLHWRKSQGIESLPPKYTW---------RGKTLNA 116 LGHE G+V E GP ++ +K GD VV+ + + G ++ + RG +NA Sbjct: 58 LGHEFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGALMNA 117 Query: 117 GLI----ATFN-------------EYAIVSENRV--TRIDPSSDMDVAALFGCAVTTGFG 157 I A F EY V + V RID + + + TG+ Sbjct: 118 KGIRSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQ 177 Query: 158 VAENNADIRLGESVVVFGAGGVGLNIIQAASLRSAYPIIAVDLHEGRLELAKQVGATHLI 217 A NA +R G SV ++GAG VG +A + A I VD H RL A++ + Sbjct: 178 -AVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPV 236 Query: 218 NASKVDARTALVELMGKQGIDVFIDNTG---QPPIIEMGYELTKAQG------RVVLVGV 268 N +VD ++ +G+D ID G + ++E K +G R + V Sbjct: 237 NFDQVDPAEYIIANTAGRGVDGVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASV 296 Query: 269 PRAG--RNISIYSLPLH-------FEKTISGSHGGETVPHVDIPRYTKLLDAGLIQLKPL 319 R G +Y+ +H FEK ++ G V H +P ++ G ++ + Sbjct: 297 RRGGVISVPGVYAGFIHGFLFGDIFEKGVTVKTGQTHVQHY-MPELLASIEEGRLRPDAI 355 Query: 320 ITEHFKLDD 328 IT L+D Sbjct: 356 ITHRLPLED 364 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 69.7 bits (169), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 86/345 (24%), Positives = 141/345 (40%), Gaps = 30/345 (8%) Query: 10 AILAQLQQPLIVDEVELPPQLEVGQVLVKIGVSGVCGSQL-----GEIDGAKGEDKYLPH 64 A++ + + L + E++LP Q+ V ++I G+CGS L G I K E P Sbjct: 3 ALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVE---APM 59 Query: 65 LLGHEASGIVQEVGPGVRFVKPGDHVVLHWRKSQGIESLPPKYTWRGKTLNAGLIATFNE 124 +LGHEASG V EVG V +K GD V + GI L T RG + + Sbjct: 60 VLGHEASGTVIEVGSAVSHLKVGDRVCME----PGIPRLDSPATLRGMYNLDPAVRFWAT 115 Query: 125 YAIVSENRVTRIDPSSDM----DVAALFGCAVTTGFGV---AENNADIRLGESVVVFGAG 177 I + + P++ D + A+ + A A ++ G++ VV GAG Sbjct: 116 PPIHGCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAG 175 Query: 178 GVGLNIIQAASLRSAYPIIAVDLHEGRLELAKQVGATHLINASKVDARTALVELMGKQGI 237 +G AA A +I D+ +L A ++ ++ + + G Sbjct: 176 TIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQATDGWGA 235 Query: 238 DVFIDNTGQPPIIEMGYELTKAQGRVVLVGVPRAGRNISIYSL---PLHFEKTISGSHGG 294 DV + +G + + +L G VLVG+P A + + ++ + E ++ Sbjct: 236 DVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYAN-- 293 Query: 295 ETVPHVDIPRYTKLLDAGLIQLKPLITEHFKLDDINVAIERMRSG 339 PR L+ +G+I +KP I+ F A E SG Sbjct: 294 ------IFPRALALISSGMIDVKPFISRKFPFSQSIRAFEEAASG 332 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.139 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,144,973 Number of extensions: 51602 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 7 Length of query: 351 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 265 Effective length of database: 1,212,660 Effective search space: 321354900 Effective search space used: 321354900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)