BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4244.p (275 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [He... 172 3e-44 gi|300309645|ref|YP_003773737.1| transketolase N-terminal subuni... 157 7e-40 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 105 3e-24 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 94 1e-20 >gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [Herbaspirillum seropedicae SmR1] Length = 281 Score = 172 bits (436), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 97/257 (37%), Positives = 136/257 (52%), Gaps = 2/257 (0%) Query: 1 MAPDITSLKTIASQLRGRIIDNAHRTQTPHLGSCLSCVDILTAAYFSTLRIDPSQPADPL 60 M+ ++ +L+ A ++R + ++G L D+L AY + + P P Sbjct: 1 MSDNLANLRASAYRIRRNAVRMGEVQGQGYIGQALGWADVLAVAYKHAMSLRPDDPQWSG 60 Query: 61 RDRFILSKGHGAAALFQVLALRGFYPQSLLDDYGKDGGIFAEHPPTPDHLPGIEAATGSL 120 RDRF+LS GH A AL+ L G P LD YG D + PG+E + GSL Sbjct: 61 RDRFLLSHGHYAIALYAALIEAGIVPDEELDSYGSDDSRLP-MSGMATYTPGMEISGGSL 119 Query: 121 GHGLPIGLGMALAGRIQQRRYDVTVLLGDGECNEGSVWEAAMMAAAQRVGNLMVLVDFNK 180 G GL IG+GMAL R V + DGE +EGS WEAAM AA ++GNL+ LVD N Sbjct: 120 GQGLAIGVGMALGLRYNNNPAFVYNSMSDGELDEGSTWEAAMGAAHHQLGNLICLVDINN 179 Query: 181 WQATGRSEEVLALNPLVDKWRAFGWDATEIDGHDMSTLVELMGRPRA-ADSKPIAVVAHT 239 QA G S +++ PL DKW AFGW +DG+D++ +V+ R D+KP ++ T Sbjct: 180 QQADGPSGKIMGFEPLADKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFDT 239 Query: 240 IKGKGVSFMEDDNNWHY 256 + GKGV F+E H+ Sbjct: 240 LMGKGVPFLEQREKNHF 256 >gi|300309645|ref|YP_003773737.1| transketolase N-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 272 Score = 157 bits (398), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 92/252 (36%), Positives = 137/252 (54%), Gaps = 4/252 (1%) Query: 1 MAPDITSLKTIASQLRGRIIDNAHRTQTPHLGSCLSCVDILTAAYFS-TLRIDPSQPADP 59 M D L+ A + + R L S LS +DI A Y+ LR +P+ P D Sbjct: 1 MKNDFVDLRAKAHWVWRETLAIHRRAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDA 60 Query: 60 LRDRFILSKGHGAAALFQVLALRGFYPQSLLDDYGKDGGIFAEHPPTPDHLPGIEAATGS 119 RDR ++SKGHG+ ++ +LA G++P L + + G F P P +PG E GS Sbjct: 61 GRDRCVISKGHGSICMYPILADLGYFPMEELQ-HVCEAGSFLGGIPDPV-IPGYETVNGS 118 Query: 120 LGHGLPIGLGMALAGRIQQRRYDVTVLLGDGECNEGSVWEAAMMAAAQRVGNLMVLVDFN 179 LGHGL + GMAL + V V+ GDGE +EG+ WEA M A+ ++ NL ++VD N Sbjct: 119 LGHGLGVATGMALGLKRSGSDRSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDN 178 Query: 180 KWQATGRSEEVLALNPLVDKWRAFGWDATEIDGHD-MSTLVELMGRPRAADSKPIAVVAH 238 + G +E++++ L + AFGWD E+DGHD ++ L+ + A KP A++A Sbjct: 179 RISMLGYTEDIVSHGELATRLSAFGWDCMEVDGHDVLAVQAALLQQKAHAAGKPKAIIAR 238 Query: 239 TIKGKGVSFMED 250 T+KG GV +E+ Sbjct: 239 TLKGHGVPGLEN 250 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 105 bits (263), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 82/273 (30%), Positives = 129/273 (47%), Gaps = 12/273 (4%) Query: 8 LKTIASQLRGRIIDNAHRTQTPHLGSCLSCVDILTAAYFSTLRIDPSQPADPLRDRFILS 67 L+ +A +R ID R H G L +I TA Y LR +P+ P RDR +LS Sbjct: 6 LQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRDRVVLS 65 Query: 68 KGHGAAALFQVLALRGFYPQSL--LDDYGKDGGIFAEHPPTPDHLPGIEAATGSLGHGLP 125 GHG+ L+ +L L G+ +L + + + G A HP L GIE TG LG G+ Sbjct: 66 NGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHCAGHPEYAPEL-GIEVTTGPLGQGIA 124 Query: 126 IGLGMALAGRIQQRRY--DVT-----VLLGDGECNEGSVWEAAMMAAAQRVGNLMVLVDF 178 GMA+A R+ D+ +GDG EG E +A R+ L +L D Sbjct: 125 NAFGMAVAEAYLNARFGDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTLLWDD 184 Query: 179 NKWQATGRSEEVLALNPLVDKWRAFGWDATEIDGHDMSTLVELMGRPRAADSKPIAVVAH 238 N+ G++E ++ N + +++R GW E+DGHD+ + + + +D +P + Sbjct: 185 NQITDDGKTELSISEN-VAERFRVAGWHVVEVDGHDIQAVSRALDAAQGSD-RPSMIACR 242 Query: 239 TIKGKGVSFMEDDNNWHYRIPNADEVAAAYQEL 271 T+ G+G+ ++ H D+ AA + L Sbjct: 243 TVIGRGIPRVQGQRGGHSARLYEDDAQAARELL 275 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 94.4 bits (233), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 80/284 (28%), Positives = 129/284 (45%), Gaps = 21/284 (7%) Query: 5 ITSLKT--IASQLRGRIIDNAHRTQTPHLGSCLSCVDILTAAYFSTLRIDPSQPADPLRD 62 I++L T +A+ +R +D + + H G + +I A + R +P+ P RD Sbjct: 2 ISTLPTDKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPANPHWANRD 61 Query: 63 RFILSKGHGAAALFQVLALRGFYPQSLLD--DYGKDGGIFAEHPPTPDHL-PGIEAATGS 119 RF+LS GHG+ + +L L G Y S+ D + + A HP H+ PG+E TG Sbjct: 62 RFVLSNGHGSMLQYALLHLTG-YDLSMEDIKSFRQLHSKTAGHPEV--HITPGVETTTGP 118 Query: 120 LGHGLPIGLGMALAGRIQQRRYD----------VTVLLGDGECNEGSVWEAAMMAAAQRV 169 LG G+ +GMALA ++ ++ +GDG EG EA +A + Sbjct: 119 LGQGITNAVGMALAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNL 178 Query: 170 GNLMVLVDFNKWQATGRSEEVLALNPLVDKWRAFGWDAT-EIDGHDMSTLVELMGRPRAA 228 L+VL D N G E + ++ A+GW+ +DGH + + + + + A Sbjct: 179 SKLIVLYDDNGISIDGHVEGWFK-DDTPKRFEAYGWNVIPAVDGHSVEAVSAAIHQAKLA 237 Query: 229 DSKPIAVVAHTIKGKGVSFMEDDNNWHYRIPNADEVAAAYQELG 272 D KP + T+ GKG + + H E+AA + LG Sbjct: 238 D-KPTLICCRTVIGKGSPNLAGTDKVHGAALGDKEIAAVREALG 280 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 978,088 Number of extensions: 44528 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 138 Number of HSP's successfully gapped: 4 Length of query: 275 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 191 Effective length of database: 1,222,130 Effective search space: 233426830 Effective search space used: 233426830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)