BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4243.p (330 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [H... 131 7e-32 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 76 4e-15 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 74 2e-14 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 64 2e-11 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 59 4e-10 >gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [Herbaspirillum seropedicae SmR1] Length = 338 Score = 131 bits (330), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 77/268 (28%), Positives = 137/268 (51%), Gaps = 22/268 (8%) Query: 64 DLAALTELIHREKPACVVNFAAQSMVAESWKNPEHWFETNVMATIRLHEVL--------- 114 D AA+ L + +P VV+ AA+S V S P + +TNV T + Sbjct: 61 DTAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSAAYDYWSTLPA 120 Query: 115 -RQCDFLDKYVHITTPEVYGSTDG---FIKEDTPFNPSTPYAVSRAAADMSLKTYFEAYG 170 Q F +++HI+T EV+GS E +P+ P++PY+ S+AA+D ++ + YG Sbjct: 121 AEQAAF--RFLHISTDEVFGSLAADAPPFTETSPYAPNSPYSASKAASDHLVRAFHSTYG 178 Query: 171 FPVVFTRAANVYGPGQQLYRIIPRTILSVLTGKKLQLHGGGLSRRSFIHMRDVSSATHAI 230 PV+ T +N YGP ++IP I + L G+ L ++G G R ++H+ D A + Sbjct: 179 LPVLITHCSNNYGPFHFPEKLIPLVITNALAGQPLPIYGDGQQVRDWLHVADHCRAIELV 238 Query: 231 ASRGVNGQTYHISTDE-------IVSVRELVERICAKLGVDFAANVEIVGERLGKDAAYM 283 +R G++Y+I + ++++ +L++ + K + + V +R G D Y Sbjct: 239 LARARPGESYNIGGNSEKTNLEVVLTLCDLLDELAPKAQGQYRDQICFVQDRPGHDRRYA 298 Query: 284 LDSSKLRQTLDWSVKISLDEGLDECVNW 311 +D+SK+++ L WS + + +GL + V W Sbjct: 299 VDTSKIQRELGWSPQHAFRQGLRQTVQW 326 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 76.3 bits (186), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 81/325 (24%), Positives = 143/325 (44%), Gaps = 21/325 (6%) Query: 3 EKYLVIGSNSFSGASFVDYLLRQGHDVIGASRSQEPHQAFLPYRWSEHQAA----FKFYP 58 +++L+ G F G++ + LL G V G QA L +E AA F+F Sbjct: 16 KRWLITGVAGFIGSNLLARLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAAAWANFRFIE 75 Query: 59 YDLNKDLAALTELIHREKPACVVNFAAQSMVAESWKNPEHWFETNVMATIRLHEVLRQCD 118 D+ A T L + V++ AA V+ S ++P NV + + R Sbjct: 76 GDI---CDAATCLRACDGADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAG 132 Query: 119 FLDKYVHITTPEVYGSTDGFIK-EDTPFNPSTPYAVSRAAADMSLKTYFEAYGFPVVFTR 177 + ++V+ + YG G K E+ P +PYAV++ ++ + + YG + R Sbjct: 133 -VARFVYAASSSTYGDHPGLPKVEEVIGKPLSPYAVTKYVNELYAEVFARCYGMESIGLR 191 Query: 178 AANVYG----PGQQLYRIIPRTILSVLTGKKLQLHGGGLSRRSFIHMRDVSSATHAIASR 233 NV+G P +IP+ + +++ +L+++G G + R F ++ +V A A+ Sbjct: 192 YFNVFGRRQDPHGAYAAVIPQWVTAMIRNGELRINGDGQTSRDFCYIDNVVQANLLAATA 251 Query: 234 G---VNGQTYHISTDEIVSVRELVERICAKLGVDFAANVE----IVGERLGKDAAYMLDS 286 G VN Q Y+++ ++ S+ +L + L F E VG R G D Sbjct: 252 GEEAVN-QVYNVALNDRTSLNDLYAMMRELLVQRFPHLQEHRPSYVGFRAGDIRDSQADI 310 Query: 287 SKLRQTLDWSVKISLDEGLDECVNW 311 K RQ L ++ L GL++ ++W Sbjct: 311 GKARQRLGYAPTHRLRAGLEQAMSW 335 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 75/336 (22%), Positives = 136/336 (40%), Gaps = 29/336 (8%) Query: 5 YLVIGSNSFSGASFVDYLLRQGHDVIGASRSQEPHQAFL--------PYRWSEHQAAFKF 56 +LV G F G++ ++ LL+ V+G Q L P +W A F+F Sbjct: 19 WLVTGVAGFIGSNLLETLLKLNQRVVGLDNFSTGFQHNLDEVKALVTPEQW----ANFRF 74 Query: 57 YPYDLNKDLAALTELIHREKPACVVNF----AAQSMVAESWKNPEHWFETNVMATIRLHE 112 D+ K + H + +V + AA V S ++P + N+ + + Sbjct: 75 VEGDIRKLDDCRAAMHHEALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLNMLV 134 Query: 113 VLRQCDFLDKYVHITTPEVYGSTDGFIK-EDTPFNPSTPYAVSRAAADMSLKTYFEAYGF 171 R + ++V+ + YG G K ED P +PYAV++ ++ + YG Sbjct: 135 AARDAR-VKRFVYAASSSTYGDHPGLPKVEDRIGKPLSPYAVTKFVNELYADVFARTYGL 193 Query: 172 PVVFTRAANVYG----PGQQLYRIIPRTILSVLTGKKLQLHGGGLSRRSFIHMRDVSSAT 227 + R N++G P +IP+ I + + G+++ ++G G + R F ++ + A Sbjct: 194 QTIGLRYFNIFGRRQNPDGAYAAVIPKWIAAAIKGEQIYINGDGETSRDFCYVDNAVQAN 253 Query: 228 HAIASR---GVNGQTYHISTDEIVSVRELVERICAKLGVDFA----ANVEIVGERLGKDA 280 A Q Y+++ + +++EL + + L F A R G Sbjct: 254 LLAACTLQDEAANQVYNVAVGDRTTLKELYQFLRDNLSATFPHLRDAQPIFREFRAGDVR 313 Query: 281 AYMLDSSKLRQTLDWSVKISLDEGLDECVNWVQQNL 316 + D K R+ L + + GL E +NW Q L Sbjct: 314 HSLADIGKGRRLLGYEPSHRIGRGLKESMNWYVQQL 349 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 77/342 (22%), Positives = 139/342 (40%), Gaps = 35/342 (10%) Query: 6 LVIGSNSFSGASFVDYLLRQGHDVIGASRSQEPHQAFLPYRWSEHQAAFKFYPYDLNKDL 65 LV G + G+ LL+ GH+VI + D+ ++ Sbjct: 4 LVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDI-RNR 62 Query: 66 AALTELIHREKPACVVNFAAQSMVAESWKNPEHWFETNVMATIRLHEVLRQCDFLDKYVH 125 L ++ K V++FA V ES + P +++ NV+ T+RL E + Q + V Sbjct: 63 EQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAM-QATGVKTIVF 121 Query: 126 ITTPEVYGSTDGF-IKEDTPFNPSTPYAVSRAAADMSLKTYFEAY-GFPVVFTRAANVYG 183 ++ VYG + E P + S PY S+ + L+ Y+ A+ + + R N G Sbjct: 122 SSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPVG 181 Query: 184 ----------PGQQLYRIIPRTILSVLTGK--KLQLHGG------GLSRRSFIHMRDVSS 225 PG ++P + V G+ +L + GG G R FIH+ D++ Sbjct: 182 AHESGLIGEDPGGIPNNLMP-FVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLAR 240 Query: 226 ATHAIASRGVNG-QTYHISTD-----EIVSVRELVERICAKLGVDFAANVEIVGERLGKD 279 H A + ++ Q + ++ ++ V + E+ C K + +A I R G Sbjct: 241 G-HVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKT-LPYA----IAERRPGDI 294 Query: 280 AAYMLDSSKLRQTLDWSVKISLDEGLDECVNWVQQNLDAMKA 321 A+ + ++ ++ L W + L+ + W Q N + KA Sbjct: 295 ASCYANPAEAKRKLGWEATLGLEAMCADSWKWQQMNPNGFKA 336 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 77/324 (23%), Positives = 131/324 (40%), Gaps = 28/324 (8%) Query: 2 SEKYLVIGSNSFSGASFVDYLLRQGHDVIGASRSQEPHQAFLPYRWSEHQAAFK---FYP 58 +K +VIG G+ VD+L+++ DV + + FL Q A K Sbjct: 5 GKKIVVIGGAGLIGSHTVDHLVKE--DV----KEILIYDNFLRGSRENLQNALKDPRVKI 58 Query: 59 YDLNKDLAALTELIHREKPACVVNFAAQSMVAESWKNPEHWFETNVMATIRLHEVLRQCD 118 YD+ D+ L + A V A + + + P F+ NV T + E Sbjct: 59 YDVGGDIMQTDILQSALEGADGVFHLAALWLLQCHEYPRSAFDVNVRGTFNVMEAC-VAK 117 Query: 119 FLDKYVHITTPEVYG-STDGFIKEDTPFNPSTPYAVSRAAADMSLKTYFEAYGFPVVFTR 177 + + V+ ++ VYG + + ED P+N Y ++ A + L+++ YG V R Sbjct: 118 GVKRLVYSSSASVYGDAVREPMDEDHPYNNKNFYGATKIAGETMLRSFHHRYGLNYVGLR 177 Query: 178 AANVYGPGQQLY----RIIPRTILSVLTGKKLQLHGGGLSRRSFIHMRDVSSATHAIASR 233 NVYGP Q + +I + + ++ G+ + G G F+ + D A Sbjct: 178 YMNVYGPRQDYHGAYIAVIMKMLDAIDKGESPTIMGDGSEAFDFVAVEDCGLANVCAMKA 237 Query: 234 GVNGQTYHISTDEIVSVRELVERICAKLGVD----FA--ANVEIVGERLGKDAAYMLDSS 287 Q Y++ T + S++EL E + G D FA + +V R+G Sbjct: 238 ETADQYYNVGTGKRTSLKELAELLLEITGCDKPITFAPRSQATLVRNRIGC-------PK 290 Query: 288 KLRQTLDWSVKISLDEGLDECVNW 311 K + ++ I L EGL + W Sbjct: 291 KASSEIGFTATIDLREGLKRLIEW 314 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,034,251 Number of extensions: 41663 Number of successful extensions: 119 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 6 Length of query: 330 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 244 Effective length of database: 1,212,660 Effective search space: 295889040 Effective search space used: 295889040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)