BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4230.p (336 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 638 0.0 gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 445 e-126 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 123 2e-29 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 102 5e-23 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 100 2e-22 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 638 bits (1645), Expect = 0.0, Method: Compositional matrix adjust. Identities = 301/336 (89%), Positives = 319/336 (94%) Query: 1 MNILVTGGAGYIGSHTCVELLNAGHEVIVFDNFCNSSPESVKRVQQITGRSLSLVKGDIR 60 MNILVTGGAGYIGSHTCVELL AGHEVIVFDNFCNSSPESVKRVQQITG++LSLVKGDIR Sbjct: 1 MNILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIR 60 Query: 61 NRDQLESVLREFKCEAVIHFAGLKAVGESVEQPLKYYDNNVVGTVRLLEAMEAAGVKTFV 120 NR+QLESVLREFKCEAVIHFAGLKAVGESVEQPL YYDNNVVGTVRLLEAM+A GVKT V Sbjct: 61 NREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIV 120 Query: 121 FSSSATVYGDPQYLPLTESHPLSASNPYGRSKIIIEDMLRDYYRAHPDWKIALLRYFNPV 180 FSSSATVYGDPQYLPL E+HPLSASNPYGRSKI+IEDMLRDYYRAHPDWKIALLRYFNPV Sbjct: 121 FSSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPV 180 Query: 181 GAHESGLIGEDPSGIPNNLMPYVAQVAVGRRERLSIWGGDYATPDGTGVRDFIHVVDLAI 240 GAHESGLIGEDP GIPNNLMP+VAQVA+GRRERL+IWGGDY TPDGTGVRDFIHVVDLA Sbjct: 181 GAHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLAR 240 Query: 241 GHVAALQALTAPECFAVNLGTGIGYSVLDVVKAYEKAAGKAIPYTIAARRPGDIASCYAD 300 GHVAALQAL AP+CFAVNLGTG+GYSVLDV+KAYEKA GK +PY IA RRPGDIASCYA+ Sbjct: 241 GHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYAN 300 Query: 301 PAEARRKLGWEAKRGLDAMCADSWRWQQNNPNGFTS 336 PAEA+RKLGWEA GL+AMCADSW+WQQ NPNGF + Sbjct: 301 PAEAKRKLGWEATLGLEAMCADSWKWQQMNPNGFKA 336 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 445 bits (1145), Expect = e-126, Method: Compositional matrix adjust. Identities = 209/327 (63%), Positives = 256/327 (78%), Gaps = 1/327 (0%) Query: 2 NILVTGGAGYIGSHTCVELLNAGHEVIVFDNFCNSSPESVKRVQQITGRSLSLVKGDIRN 61 ILVTGGAGYIGSHTCVEL++AG+ V+V DN CNS + R+ QI+G+ V+GDIR+ Sbjct: 5 TILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQGDIRD 64 Query: 62 RDQLESVLREFKCEAVIHFAGLKAVGESVEQPLKYYDNNVVGTVRLLEAMEAAGVKTFVF 121 R L+ + + + EAVIHFAGLKAVGESV QPL+YYDNNV G+ L EAM A GVK VF Sbjct: 65 RAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVKNIVF 124 Query: 122 SSSATVYGDPQYLPLTESHPLSASNPYGRSKIIIEDMLRDYYRAHPDWKIALLRYFNPVG 181 SSSATVYGDP +P+TE PLSA+NPYGRSK+++E +L D + A P W+IALLRYFNPVG Sbjct: 125 SSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYFNPVG 184 Query: 182 AHESGLIGEDPSGIPNNLMPYVAQVAVGRRERLSIWGGDYATPDGTGVRDFIHVVDLAIG 241 AHESGLIGEDPSGIPNNL+P++AQVA GRR LS++G DY TPDGTGVRD+IHVVDLA+G Sbjct: 185 AHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVDLALG 244 Query: 242 HVAALQAL-TAPECFAVNLGTGIGYSVLDVVKAYEKAAGKAIPYTIAARRPGDIASCYAD 300 H+ L+ L P + NLGTG G SVL+++ A+E+A G +PY + RRPGDIA CYA Sbjct: 245 HLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIACCYAA 304 Query: 301 PAEARRKLGWEAKRGLDAMCADSWRWQ 327 A R+LGW A+RG++AMCAD+WRWQ Sbjct: 305 TERAERELGWRAQRGIEAMCADTWRWQ 331 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 106/335 (31%), Positives = 161/335 (48%), Gaps = 31/335 (9%) Query: 3 ILVTGGAGYIGSHTCVELLNAG-HEVIVFDNFCNSSPESVKRVQQITGRSLSLVKGDIRN 61 I+V GGAG IGSHT L+ E++++DNF S E+++ + + V GDI Sbjct: 8 IVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGSRENLQNALKDPRVKIYDVGGDIMQ 67 Query: 62 RDQLESVLREFKCEAVIHFAGLKAVGESVEQPLKYYDNNVVGTVRLLEAMEAAGVKTFVF 121 D L+S L + V H A L + + E P +D NV GT ++EA A GVK V+ Sbjct: 68 TDILQSALE--GADGVFHLAALWLL-QCHEYPRSAFDVNVRGTFNVMEACVAKGVKRLVY 124 Query: 122 SSSATVYGDPQYLPLTESHPLSASNPYGRSKIIIEDMLRDYYRAHPDWKIALLRYFNPVG 181 SSSA+VYGD P+ E HP + N YG +KI E MLR ++ + + LRY N G Sbjct: 125 SSSASVYGDAVREPMDEDHPYNNKNFYGATKIAGETMLRSFHHRYGLNYVG-LRYMNVYG 183 Query: 182 AHESGLIGEDPSGIPNNLMPYVAQV-----AVGRRERLSIWGGDYATPDGTGVRDFIHVV 236 +D G Y+A + A+ + E +I G DG+ DF+ V Sbjct: 184 PR------QDYHG------AYIAVIMKMLDAIDKGESPTIMG------DGSEAFDFVAVE 225 Query: 237 DLAIGHVAALQALTAPECFAVNLGTGIGYSVLDVVKAYEKAAGKAIPYTIAARRPGDIA- 295 D + +V A++A TA + + N+GTG S+ ++ + + G P T A R + Sbjct: 226 DCGLANVCAMKAETADQYY--NVGTGKRTSLKELAELLLEITGCDKPITFAPRSQATLVR 283 Query: 296 SCYADPAEARRKLGWEAKRGLDAMCADSWRWQQNN 330 + P +A ++G+ A L W+ N+ Sbjct: 284 NRIGCPKKASSEIGFTATIDLREGLKRLIEWRANH 318 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 102 bits (253), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 93/341 (27%), Positives = 148/341 (43%), Gaps = 34/341 (9%) Query: 4 LVTGGAGYIGSHTCVELLNAGHEVIVFDNFCNSSPESVKRVQQITG----RSLSLVKGDI 59 LVTG AG+IGS+ LL V+ DNF ++ V+ + + V+GDI Sbjct: 20 LVTGVAGFIGSNLLETLLKLNQRVVGLDNFSTGFQHNLDEVKALVTPEQWANFRFVEGDI 79 Query: 60 RNRDQLESVLREFK-----CEAVIHFAGLKAVGESVEQPLKYYDNNVVGTVRLLEAMEAA 114 R D + + E V+H A L +V S+E P+ NN+ G + +L A A Sbjct: 80 RKLDDCRAAMHHEALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLNMLVAARDA 139 Query: 115 GVKTFVFSSSATVYGDPQYLPLTESHPLSASNPYGRSKIIIEDMLRDYYRAHPDWKIALL 174 VK FV+++S++ YGD LP E +PY +K + E + R + I L Sbjct: 140 RVKRFVYAASSSTYGDHPGLPKVEDRIGKPLSPYAVTKFVNELYADVFARTYGLQTIG-L 198 Query: 175 RYFNPVGAHESGLIGEDPSGIPNNLMPYVAQVAVGRRERLSIWGGDYATPDGTGVRDFIH 234 RYFN G ++P G ++P A+ + E++ I G DG RDF + Sbjct: 199 RYFNIFGRR------QNPDGAYAAVIPKWIAAAI-KGEQIYING------DGETSRDFCY 245 Query: 235 VVDLAIGHVAA---LQALTAPECFAVNLGTGIGYSVLDVVKAYEKAAGKAIPYTIAAR-- 289 V + ++ A LQ A + + N+ G ++ ++ + P+ A+ Sbjct: 246 VDNAVQANLLAACTLQDEAANQVY--NVAVGDRTTLKELYQFLRDNLSATFPHLRDAQPI 303 Query: 290 ----RPGDIASCYADPAEARRKLGWEAKRGLDAMCADSWRW 326 R GD+ AD + RR LG+E + +S W Sbjct: 304 FREFRAGDVRHSLADIGKGRRLLGYEPSHRIGRGLKESMNW 344 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 100 bits (249), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 97/337 (28%), Positives = 148/337 (43%), Gaps = 34/337 (10%) Query: 4 LVTGGAGYIGSHTCVELLNAGHEVIVFDNFCNSSPESVKRVQQITGRS----LSLVKGDI 59 L+TG AG+IGS+ LL G +V DNF +++ V+ G + ++GDI Sbjct: 19 LITGVAGFIGSNLLARLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAAAWANFRFIEGDI 78 Query: 60 RNRDQLESVLREFK-CEAVIHFAGLKAVGESVEQPLKYYDNNVVGTVRLLEAMEAAGVKT 118 + + LR + V+H A L +V S+E P++ NNV G + +L A AGV Sbjct: 79 CD---AATCLRACDGADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAGVAR 135 Query: 119 FVFSSSATVYGDPQYLPLTES---HPLSASNPYGRSKIIIEDMLRDYYRAHPDWKIALLR 175 FV+++S++ YGD LP E PLS PY +K + E + R + I LR Sbjct: 136 FVYAASSSTYGDHPGLPKVEEVIGKPLS---PYAVTKYVNELYAEVFARCYGMESIG-LR 191 Query: 176 YFNPVGAHESGLIGEDPSGIPNNLMPYVAQVAVGRRERLSIWGGDYATPDGTGVRDFIHV 235 YFN G +DP G ++P A+ R L I G DG RDF ++ Sbjct: 192 YFNVFGRR------QDPHGAYAAVIPQWV-TAMIRNGELRING------DGQTSRDFCYI 238 Query: 236 VDLAIGHVAALQALTAPECFAVNLGTGIGYSVLDVVKAYEKAAGKAIPYTIAAR------ 289 ++ ++ A A N+ S+ D+ + + P+ R Sbjct: 239 DNVVQANLLAATAGEEAVNQVYNVALNDRTSLNDLYAMMRELLVQRFPHLQEHRPSYVGF 298 Query: 290 RPGDIASCYADPAEARRKLGWEAKRGLDAMCADSWRW 326 R GDI AD +AR++LG+ L A + W Sbjct: 299 RAGDIRDSQADIGKARQRLGYAPTHRLRAGLEQAMSW 335 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,145,383 Number of extensions: 50424 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 8 Length of query: 336 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 250 Effective length of database: 1,212,660 Effective search space: 303165000 Effective search space used: 303165000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)