BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4208.p (1247 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313633|ref|YP_003777725.1| methionine synthase I, cobalami... 2548 0.0 >gi|300313633|ref|YP_003777725.1| methionine synthase I, cobalamin-binding domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1247 Score = 2548 bits (6603), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1226/1247 (98%), Positives = 1240/1247 (99%) Query: 1 MKPNELCQTELLLRDLMSQRILILDGAMGTMIQRYKLTEEDYRGQRFADFSVPGKELFVK 60 MKPNELCQTELLLRDLMSQRILILDGAMGTMIQRYKLTEEDYRGQRFADFSVPGK+LFVK Sbjct: 1 MKPNELCQTELLLRDLMSQRILILDGAMGTMIQRYKLTEEDYRGQRFADFSVPGKDLFVK 60 Query: 61 GNNELLSLTQPHIIQEIHEQYLAAGADLIETNTFGATSVAQDDYHMAHLVYEMNLESARL 120 GNNELLSLTQPHIIQEIHEQYLAAGADLIETNTFGATSVAQDDYHMAHLVYEMN+ESARL Sbjct: 61 GNNELLSLTQPHIIQEIHEQYLAAGADLIETNTFGATSVAQDDYHMAHLVYEMNVESARL 120 Query: 121 ARAACDKYATPDKPRFVAGALGPTPKTASISPDVNDPAARNVTFDQLVTAYLEQTRALVE 180 ARAACDKYATPDKPRFVAGALGPTPKTASISPDVNDPAARNVTFDQLV AYLEQTRALVE Sbjct: 121 ARAACDKYATPDKPRFVAGALGPTPKTASISPDVNDPAARNVTFDQLVAAYLEQTRALVE 180 Query: 181 GGADVLLVETIFDTLNCKAALFAIDTFFEESGKRLPIMISGTVTDASGRILSGQTVTAFW 240 GGADVLLVETIFDTLNCKAALFAIDTFFEESG+RLPIMISGTVTDASGRILSGQTVTAFW Sbjct: 181 GGADVLLVETIFDTLNCKAALFAIDTFFEESGQRLPIMISGTVTDASGRILSGQTVTAFW 240 Query: 241 NSIRHARPLTVGLNCALGAALMRPYAEELSKIADTYVCIYPNAGLPNPMSDTGFDETPDV 300 NSIRHARPLTVGLNCALGAALMRPYAEELSKIADT+VCIYPNAGLPNPMSDTGFDETPDV Sbjct: 241 NSIRHARPLTVGLNCALGAALMRPYAEELSKIADTFVCIYPNAGLPNPMSDTGFDETPDV 300 Query: 301 TSALLKEFAESGFVNVAGGCCGTTPPHIKAIAETVAKIAPRKVPEPTHEMRLSGLEPFTI 360 TSALLKEFAESGFVNVAGGCCGTTPPHIKAIAETVAKIAPRKVPEPTHEMRLSGLEPFTI Sbjct: 301 TSALLKEFAESGFVNVAGGCCGTTPPHIKAIAETVAKIAPRKVPEPTHEMRLSGLEPFTI 360 Query: 361 NDDSLYVNVGERTNVTGSKAFARLILNEQYDEALAVARQQVENGAQIIDINMDEAMLDSV 420 NDDSLYVNVGERTNVTGSKAFARLILNEQYDEALAVARQQVENGAQIIDINMDEAMLDSV Sbjct: 361 NDDSLYVNVGERTNVTGSKAFARLILNEQYDEALAVARQQVENGAQIIDINMDEAMLDSV 420 Query: 421 AAMTRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKSIVNSISMKEGEEKFLREA 480 AAMTRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKSIVNSISMKEGEEKFLREA Sbjct: 421 AAMTRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKSIVNSISMKEGEEKFLREA 480 Query: 481 KLCRRYGAAVIVMAFDEVGQADTFARKIEICERAYRLLVDKLDFPPEDIIFDPNIFAVAT 540 KLCRRYGAAVIVMAFDEVGQADTFARKIEICERAYRLLVDKLDFPPEDIIFDPNIFAVAT Sbjct: 481 KLCRRYGAAVIVMAFDEVGQADTFARKIEICERAYRLLVDKLDFPPEDIIFDPNIFAVAT 540 Query: 541 GIEEHNNYAVDFIEATRWIHQNLPYAKISGGVSNVSFSFRGNDPAREAIHTVFLYHAIKA 600 GIEEHNNYAVDFIEATRWIHQNLPYAKISGGVSNVSFSFRGNDPAREAIHTVFLYHAIKA Sbjct: 541 GIEEHNNYAVDFIEATRWIHQNLPYAKISGGVSNVSFSFRGNDPAREAIHTVFLYHAIKA 600 Query: 601 GMTMGIVNAGMIGVYDDLPAELRERVEDVVLNRREDATERMIEYAATLKAGDKKEEATLE 660 GMTMGIVNAGMIGVYDDLPAELRERVEDVVLNRREDATERMIEYAATLKAGDKKEEATLE Sbjct: 601 GMTMGIVNAGMIGVYDDLPAELRERVEDVVLNRREDATERMIEYAATLKAGDKKEEATLE 660 Query: 661 WRNLPVAKRLSHALVHGITQWIVEDTEEVRQQIAADGGRPIHVIEGPLMDGMNVVGDLFG 720 WRNLPVAKRLSHALVHGITQWIVEDTEEVRQQIAADGGRPIHVIEGPLMDGMNVVGDLFG Sbjct: 661 WRNLPVAKRLSHALVHGITQWIVEDTEEVRQQIAADGGRPIHVIEGPLMDGMNVVGDLFG 720 Query: 721 QGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRQLEIATGEVVKPKGKIVIATVKGDVHDI 780 QGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRQLEIATGEV KPKGKIVIATVKGDVHDI Sbjct: 721 QGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRQLEIATGEVAKPKGKIVIATVKGDVHDI 780 Query: 781 GKNIVTVVLQCNNFEVVNMGVMVPCAEILAKAKEEKADIIGLSGLITPSLEEMAYVAKEM 840 GKNIVTVVLQCNNFEVVNMGVMVPCAEILAKAKEEKADIIGLSGLITPSLEEMAYVAKEM Sbjct: 781 GKNIVTVVLQCNNFEVVNMGVMVPCAEILAKAKEEKADIIGLSGLITPSLEEMAYVAKEM 840 Query: 841 QRDPYFAGLKMPLMIGGATTSRAHTAVKIAPNYEGPVVYVPDASRAVSVAQSLLADEQKT 900 QRDPYFAGLKMPLMIGGATTSRAHTAVKIAPNYEGPVVYVPDASRAVSVAQSLLADEQKT Sbjct: 841 QRDPYFAGLKMPLMIGGATTSRAHTAVKIAPNYEGPVVYVPDASRAVSVAQSLLADEQKT 900 Query: 901 QYVAELNADYDRIREQHASKKAAPMLSLAAARANKTKLEFAPVKPKFIGRRLFKNVDLGL 960 QYVAELNADY+RIREQHASKKAAPMLSLAAARANKTKL+FAPVKPKFIGRRLFKNVDLGL Sbjct: 901 QYVAELNADYERIREQHASKKAAPMLSLAAARANKTKLDFAPVKPKFIGRRLFKNVDLGL 960 Query: 961 LANYIDWGPFFQTWDLAGPYPAILTDEVVGEAATKVYQEAQAMLKKIIDGRWLTANGVIS 1020 LANYIDWGPFFQTWDLAGPYPAILTDEVVGEAATKV+QEAQAMLKKIIDGRWLTANGVIS Sbjct: 961 LANYIDWGPFFQTWDLAGPYPAILTDEVVGEAATKVFQEAQAMLKKIIDGRWLTANGVIS 1020 Query: 1021 LLPANTVNEDDIEIYTDDSRSQVAFTYYGLRQQTEKPVVDGVARPNQCLSDFIAPKDSGV 1080 LLPANTVN+DDIEIYTDDSRSQVAFTYYGLRQQTEKPVVDGVARPNQCLSDFIAPK+SGV Sbjct: 1021 LLPANTVNDDDIEIYTDDSRSQVAFTYYGLRQQTEKPVVDGVARPNQCLSDFIAPKESGV 1080 Query: 1081 PDYIGMFAVTAGLGIEKYEKRFEDAHDDYSSIMLKALADRLAEAFAEYLHERVRKDLWGY 1140 DYIGMFAVTAGLGIEKYEKRFEDAHDDYSSIMLKALADRLAEAFAEYLHERVRKDLWGY Sbjct: 1081 QDYIGMFAVTAGLGIEKYEKRFEDAHDDYSSIMLKALADRLAEAFAEYLHERVRKDLWGY 1140 Query: 1141 AADENLDSAELIKEKYLGIRPAPGYPACPEHTVKADAFRVMQCDEIGMQLTESYAMFPGA 1200 AADENL S +LIKEKYLGIRPAPGYPACPEHTVKAD FR MQCDEIGMQLTESYAMFPGA Sbjct: 1141 AADENLSSTDLIKEKYLGIRPAPGYPACPEHTVKADVFRTMQCDEIGMQLTESYAMFPGA 1200 Query: 1201 SVSGFYFAHPQSKYFVVGKIGEDQVIDMAQRRHVPKEDVERWLAPNL 1247 SVSGFYFAHPQSKYFVVGKIGEDQV+DMA+RRHVPKE++ERWLAPNL Sbjct: 1201 SVSGFYFAHPQSKYFVVGKIGEDQVVDMAERRHVPKEELERWLAPNL 1247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,821,669 Number of extensions: 161793 Number of successful extensions: 494 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 494 Number of HSP's successfully gapped: 1 Length of query: 1247 Length of database: 1,619,870 Length adjustment: 96 Effective length of query: 1151 Effective length of database: 1,165,310 Effective search space: 1341271810 Effective search space used: 1341271810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 136 (57.0 bits)