BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4087.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313509|ref|YP_003777601.1| hypothetical protein Hsero_4224... 153 9e-39 >gi|300313509|ref|YP_003777601.1| hypothetical protein Hsero_4224 [Herbaspirillum seropedicae SmR1] Length = 242 Score = 153 bits (387), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 131/229 (57%), Positives = 161/229 (70%), Gaps = 4/229 (1%) Query: 1 MDKALWNFLLPHWDGSAYGGLQCLLALLALAPVIALPLLLARTAQPAQWQARLIRIADQP 60 MD+++ FLLP W + GGLQ + ALL + +I +P L+ R A PAQWQ R ++ P Sbjct: 14 MDRSVLGFLLPQWQAAGEGGLQLVFALLFVLALILMPWLVRRAATPAQWQQRWAALSADP 73 Query: 61 ASLPLTTTPEQLSQAVATAAERWAVVLPGLMLMLGLLGTFIGLGLAL-SAVGVPD---AQ 116 SLP + TPEQLSQ VAT ERWA ++PGL+LM GLLGTFIGLGLAL A G D A Sbjct: 74 QSLPSSITPEQLSQIVATGPERWARLVPGLLLMAGLLGTFIGLGLALGEASGALDGAQAP 133 Query: 117 AALGFVIDALGSQFKSAVWGLLAFLILKSWNTVRPHEQARLAWSLATLQTLTDAAVQRQS 176 AL V+D LG++F+ A WG+L++LIL+ W HEQARLAWS A L L AVQRQ Sbjct: 134 TALAAVLDTLGAKFRIAAWGILSYLILRLWWAASAHEQARLAWSAAALGALAAQAVQRQV 193 Query: 177 QQQAQQQQRLVEAITQSGNALLVAQQAEAQRAHLRHGELLDALQQTTAR 225 QQ+ QQQRL+EAI+ +G ALL AQQAEAQRAH+RH EL+DALQ+ AR Sbjct: 194 QQEQAQQQRLIEAISHNGQALLAAQQAEAQRAHVRHAELVDALQRQVAR 242 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.131 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 562,992 Number of extensions: 15916 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 122 Number of HSP's successfully gapped: 1 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)