BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4083.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein... 82 3e-17 gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogena... 80 2e-16 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 75 6e-15 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 75 7e-15 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 74 8e-15 >gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 283 Score = 82.4 bits (202), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 83/253 (32%), Positives = 117/253 (46%), Gaps = 23/253 (9%) Query: 5 RRILITGATGAIGSALAIRYAAPGIHLLLQGRRSELLAGLQWQCLERGAAVETVTLDLCD 64 RRI + GA+ IG+ LA + A G + L RR+E+L + E A V LC Sbjct: 39 RRIWLIGASSGIGAELARQALAAGARVALSARRAEVLEEVAGAHPEAFIAPLDV---LCH 95 Query: 65 RQELAAWLDQICQAPLPELVILNAGLNTHI--GPRGEPE-PWAQVEAVMEVNLR---ASI 118 AW DQ + +V G++ I R PE W E E +R AS+ Sbjct: 96 D----AWRDQHAR-----IVEAFGGVDLLIFCAARYRPERSWEVQEDEAEHTIRTNLASV 146 Query: 119 FIA-QRLLPGMLARGYGQLALMSSLAACFGLPVTPAYCATKAGLKAYGQALRGWLSPRGI 177 + A LP MLARG G +AL++S+A GLP Y KA L + L L P+G+ Sbjct: 147 YSALGAALPDMLARGSGGIALVASVAGYVGLPGATVYGPGKAALINLAEILYSDLRPKGV 206 Query: 178 EVNVILPGYVNSPMCHDMPGPKPFLWSARRAAQYIQHGLARNRASISFPFPLNWGAW--W 235 V ++ PG+V + + P L + ++AA I G+ R I FPL + AW Sbjct: 207 NVYLVNPGFVKTDLTDKNDFTMPALQTPQQAASAIWRGIGEGRFEIH--FPLRFTAWLKL 264 Query: 236 LQILPTALSLRIL 248 L+ILP L + Sbjct: 265 LRILPFRLRFMLF 277 >gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 268 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 77/250 (30%), Positives = 119/250 (47%), Gaps = 24/250 (9%) Query: 6 RILITGATGAIGSALAIRYAAPGIHLLLQGRRSELLAGLQWQCLERGAAVETVTLDLCDR 65 R+ +TGA+ +G ALA YA+ G L L RR+ L L+ L LD+ D Sbjct: 12 RVFLTGASSGLGMALARAYASQGAVLGLVARRTAELEALR-AALPHAERHRVYALDVTDH 70 Query: 66 QEL----AAWLDQICQAPLPELVILNAGLNTHIGPRGE-PEPWAQVEAVMEVNLRASI-- 118 L +++D+ A ++VI NAG++ G E E A E ++ VN+ A+ Sbjct: 71 AALAAAATSFMDEFGGA---DVVIANAGISQ--GTLTEFQEDLAAFERILAVNVTATFAT 125 Query: 119 ---FIAQRLLPGMLARGYGQLALMSSLAACFGLPVTPAYCATKAGLKAYGQALRGWLSPR 175 F+A+ R +L + S+A GLP AY A+K+ + +Y ++LR L Sbjct: 126 FTPFVARMKASPEAQRRRCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLRVELRAS 185 Query: 176 GIEVNVILPGYVNSPM----CHDMPGPKPFLWSARRAAQYIQHGLARNRASISFPFPLNW 231 GI+V I PGY+++PM + MP P A RA Q I G++ P+ + Sbjct: 186 GIKVVTIAPGYIDTPMTRVNTYRMPFLMPVERFAARAVQTIDEGVSYR----VIPWQMGV 241 Query: 232 GAWWLQILPT 241 A L++LP Sbjct: 242 VAKLLRMLPN 251 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 74.7 bits (182), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 55/196 (28%), Positives = 91/196 (46%), Gaps = 5/196 (2%) Query: 8 LITGATGAIGSALAIRYAAPGIHLLLQGRRSELLAGLQWQCLERGAAVETVTLDLCDRQE 67 LITG++G IG ALA A G ++L GR + LA + Q G + + D +E Sbjct: 23 LITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSFDATSSEE 82 Query: 68 LAAWLDQICQAPLPELVILNAGLNTHIGPRGEPEPWAQVE--AVMEVNLRASIFIAQRLL 125 + A + +I P +++N N I R E + + + ++ NL + F AQ + Sbjct: 83 VKAAVQKIESEIGPIGILVN---NAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQAVA 139 Query: 126 PGMLARGYGQLALMSSLAACFGLPVTPAYCATKAGLKAYGQALRGWLSPRGIEVNVILPG 185 M+ RG G++ + S+ + G P Y ATK +K + + + GI+VN + PG Sbjct: 140 RHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPG 199 Query: 186 YVNSPMCHDMPGPKPF 201 Y + M + F Sbjct: 200 YFKTEMNMALVNDAKF 215 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 74.7 bits (182), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 63/204 (30%), Positives = 98/204 (48%), Gaps = 14/204 (6%) Query: 8 LITGATGAIGSALAIRYAAPGIHLLLQGRRSELLAGLQWQCLERGAAVETVTLDLCDRQE 67 LITGA+ IG A A+ +A G L+L RR + L + +G V D+ + Sbjct: 10 LITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAGDVGE--- 66 Query: 68 LAAWLDQICQAPLPELVILNAGLNTHIGPRGEPEPWAQV-----EAVMEVNLRASIFIAQ 122 AA ++ L E L+ +N + G G +P A++ + + NL ++ A+ Sbjct: 67 -AATHERGVAMALDEFGGLDIAIN-NAGAVGACKPLAEISPQQWQDTLHANLTSAFLGAR 124 Query: 123 RLLPGMLARGYGQLALMSS-LAACFGLPVTPAYCATKAGLKAYGQALRGWLSPRGIEVNV 181 +P MLARG G + SS + + GLP AY A KAGL + + + +GI N Sbjct: 125 CQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRANA 184 Query: 182 ILPGYVNSPMCHDMPGPKPFLWSA 205 +LPG V++ M D P+ W+A Sbjct: 185 LLPGGVDTDMGGD---PQQKQWAA 205 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 74.3 bits (181), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 58/183 (31%), Positives = 89/183 (48%), Gaps = 10/183 (5%) Query: 7 ILITGATGAIGSALAIRYAAPGIHLLLQGRRSELLAGLQWQCLERGA-AVETVTLDLCDR 65 +LITGAT G+A+A ++A G ++ GRR E L L E GA V + LD+ + Sbjct: 3 VLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDL---VKELGADKVLPLVLDVTSK 59 Query: 66 QELAAWLDQICQA-PLPELVILNAGLNTHIGPRGEP--EPWAQVEAVMEVNLRASIFIAQ 122 + L + A +++I NAGL +GP E W E +++ N++ + I Sbjct: 60 SSIETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDW---ETMIDTNVKGLVTITH 116 Query: 123 RLLPGMLARGYGQLALMSSLAACFGLPVTPAYCATKAGLKAYGQALRGWLSPRGIEVNVI 182 LLP M+ RG G + + S+A + P Y ATKA + + LR L G+ + Sbjct: 117 ALLPDMVKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNL 176 Query: 183 LPG 185 PG Sbjct: 177 APG 179 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.457 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 926,668 Number of extensions: 39755 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 253 Number of HSP's successfully gapped: 24 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 129 (54.3 bits)