BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4049.p (188 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313467|ref|YP_003777559.1| type IV pilus biogenesis protei... 56 2e-09 >gi|300313467|ref|YP_003777559.1| type IV pilus biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 186 Score = 56.2 bits (134), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 93/190 (48%), Positives = 117/190 (61%), Gaps = 14/190 (7%) Query: 1 MSCGFDFSCSPAQRRRIRERNFYRALSAAVLLGCLLALLPGARIFLHTRSFQEANALLRQ 60 M+ DF+ PA RRR RE+ F+ L AA LLG LLA+ PG RI LH ANALL Q Sbjct: 1 MNSVIDFAHPPALRRRRREQRFHGWLCAAALLGVLLAMAPGLRIHLHQGRLAAANALLLQ 60 Query: 61 ALHSLAPQVQQTAALRQRIEALEKQLAAQQQLTLRRQQASLLLRSAAQAAQAANSPL-RL 119 +LA +Q A L+ RI AL+++LA QQQL RR QA LLLR A+ A+ PL L Sbjct: 61 EAQALASATRQAAELQTRISALQQRLAQQQQLARRRTQAGLLLRGVAR----ASLPLVHL 116 Query: 120 QRILLQADRAELRGQAASAQELRDFTNALASAGLEGATLNDLQAEDGA----YAFSLIIP 175 +R+ LQ D AELRG+A S +++ ALA+AGLEGA L DL+ + A Y++SL IP Sbjct: 117 ERLTLQKDHAELRGRAGSMAAIQELQGALAAAGLEGAHLLDLRTQASAAAEGYSWSLSIP 176 Query: 176 L-----APPP 180 L A PP Sbjct: 177 LLAAGAASPP 186 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.131 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 362,891 Number of extensions: 9127 Number of successful extensions: 92 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 91 Number of HSP's successfully gapped: 1 Length of query: 188 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 108 Effective length of database: 1,241,070 Effective search space: 134035560 Effective search space used: 134035560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)