BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4043.p (334 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313461|ref|YP_003777553.1| prolin-rich signal peptide prot... 349 2e-97 gi|300313462|ref|YP_003777554.1| hypothetical protein Hsero_4176... 176 3e-45 >gi|300313461|ref|YP_003777553.1| prolin-rich signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 255 Score = 349 bits (895), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 162/224 (72%), Positives = 188/224 (83%) Query: 107 APATGKQYHTSAPPSALLQYDVTGTKDQQPAYGRGSIDWHNEGNHYRVEGKAKALFFTLL 166 A TGKQY T APPSA+L YDVTGT DQ+P+YG S+DW N G HYR+EG+ A++ T L Sbjct: 31 ADTTGKQYRTDAPPSAVLDYDVTGTIDQKPSYGSASLDWRNSGQHYRLEGEVTAIWVTWL 90 Query: 167 NFTSVGELDAWGVSPELYTEQKGFKSATNTHFNRARNLVSFSASTISYPRRGGEQDRASL 226 F+S GELDAWGVSP LYTE+K F+SATNTHFNR + +SFSAS YPRRGGEQDR SL Sbjct: 91 KFSSEGELDAWGVSPTLYTEKKKFRSATNTHFNRNGDSISFSASETRYPRRGGEQDRGSL 150 Query: 227 IWQLAAIGRGDATQIQPGAVIDLFVAGVRDGELWRVQVLGQEALDLPIGHLQTWHLVRMP 286 IWQLAAIGRGD Q QPGAVIDLFVAGVRDGE+WR+QV+G+EA+ LPIG LQTWH+VR+P Sbjct: 151 IWQLAAIGRGDPAQFQPGAVIDLFVAGVRDGEVWRMQVIGEEAIKLPIGTLQTWHVVRVP 210 Query: 287 KPGSYDDRVDIWLAPQHEWYPVRVRYTDLRPPGDYTQLDLSELK 330 KPG+Y+DR+DIWLAPQHEWYP R+RYTD RP GD T+L LSEL+ Sbjct: 211 KPGAYEDRIDIWLAPQHEWYPARLRYTDPRPNGDVTELSLSELR 254 >gi|300313462|ref|YP_003777554.1| hypothetical protein Hsero_4176 [Herbaspirillum seropedicae SmR1] Length = 253 Score = 176 bits (445), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 85/213 (39%), Positives = 127/213 (59%), Gaps = 3/213 (1%) Query: 119 PPSALLQYDVTGTKDQQPAYGRGSIDWHNEGNHYRVEGKAKALFF-TLLNFTSVGELDAW 177 PPSA L Y + + G+G + W Y V + +A+ +L TS G +DA+ Sbjct: 40 PPSAELNYSIQAKQSGLDVKGQGLVKWTQSKTSYSVTSETRAMLLGKILEATSEGAVDAY 99 Query: 178 GVSPELYTEQKGFKSATNTHFNRARNLVSFSASTISYPRRGGEQDRASLIWQLAAIGRGD 237 G++P+ + E++ + + T FNR +N ++F+ S SYP +GGEQDR S+ WQ AI R + Sbjct: 100 GLAPDRFVEKRLRREPSTTTFNREQNKITFTQSAESYPLQGGEQDRTSITWQFVAIARAN 159 Query: 238 ATQIQPGAVIDLFVAGVRDGELWRVQVLGQEALDLPIGHLQTWHLVRMPKPGSYDDRVDI 297 A +++PG+ + FVAG RD E W +V+G+E + P G + HL R P P ++DI Sbjct: 160 AAKMKPGSEWNFFVAGQRDAEQWTFKVVGREKIRTPQGETEALHLFRAPPPDDQGQKLDI 219 Query: 298 WLAPQHEWYPVRVRYTDLRPPGDYTQLDLSELK 330 WLAP+ EWYPVR+++TD P GDY + L LK Sbjct: 220 WLAPKMEWYPVRLKFTD--PDGDYIEQTLDSLK 250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.132 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,042,890 Number of extensions: 43541 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 2 Length of query: 334 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 248 Effective length of database: 1,212,660 Effective search space: 300739680 Effective search space used: 300739680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)