BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4009.p (454 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313429|ref|YP_003777521.1| zinc protease-like signal pepti... 729 0.0 gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbasp... 93 6e-20 gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum s... 63 5e-11 >gi|300313429|ref|YP_003777521.1| zinc protease-like signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 438 Score = 729 bits (1881), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/412 (87%), Positives = 384/412 (93%), Gaps = 2/412 (0%) Query: 40 IQSWTQPDGARVLLVSNHAIPMLDVSVQFDAGRRRDPAAKAGLAELTVASLTRGVEAADG 99 IQSWTQPDGARVL V NHAIPMLDVSVQFDAG+RRDPA KAGLAELTVASLTRGV A G Sbjct: 26 IQSWTQPDGARVLFVPNHAIPMLDVSVQFDAGQRRDPAGKAGLAELTVASLTRGVTDASG 85 Query: 100 KPALNEAQILDGFADVAAQQQGGAGQDRAGVSLRTLSSPAERDAALGLLARILAQPSFPQ 159 L EAQILDGFADVAAQQ GAGQDRAGVSLRTLSSPAER+AAL LLAR+LA PSFPQ Sbjct: 86 --TLTEAQILDGFADVAAQQHDGAGQDRAGVSLRTLSSPAEREAALTLLARMLAHPSFPQ 143 Query: 160 AALERDRALSIANLKEELTKPEAIAEKAFMQAAYGHHPYAIEASPATLQAITRDDLLAFH 219 A+LERDRAL+IAN+KEELTKPE IAEKAFM AAYG HPYA++AS A++QAITR+DL AFH Sbjct: 144 ASLERDRALAIANIKEELTKPEVIAEKAFMHAAYGSHPYAMDASEASMQAITREDLQAFH 203 Query: 220 RSHYVANRAVIAMIGDITPEQARTMASALTRALPQGAALPALPPVPAPQGSEQRIAHPAS 279 R+HYVANRAVIA+IGDI EQAR +ASALTR LPQGAALPALPPV AP+GSE+RIAHPAS Sbjct: 204 RAHYVANRAVIALIGDINLEQARAIASALTRELPQGAALPALPPVVAPKGSEERIAHPAS 263 Query: 280 QSHILIGAPALQRGDPDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGFSPLAQ 339 QSHILIGAPA+QRGDPDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGFSPLAQ Sbjct: 264 QSHILIGAPAIQRGDPDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGFSPLAQ 323 Query: 340 PGPFQIGLQTKKEQTAEALRVTRATLDRFLQEGPTSAELKAAKDNLTGGFALRIDSNAKL 399 PGPFQIGLQTKKEQTAEALRVTR TLD+F+QEGPT+AELKAAKDNL GGFALRIDSNAKL Sbjct: 324 PGPFQIGLQTKKEQTAEALRVTRVTLDKFMQEGPTAAELKAAKDNLAGGFALRIDSNAKL 383 Query: 400 LENLAVIGFYGLPLDYLDHWIERVRAVSVQDVRAAFGKHVHPQALSTVIVGE 451 LENL+VIGFYGLPLDYLDHWIER+RAVSVQDVRAAF KHVHP+ LST+IVGE Sbjct: 384 LENLSVIGFYGLPLDYLDHWIERIRAVSVQDVRAAFRKHVHPEELSTIIVGE 435 >gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbaspirillum seropedicae SmR1] Length = 929 Score = 92.8 bits (229), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 100/416 (24%), Positives = 172/416 (41%), Gaps = 63/416 (15%) Query: 62 LDVSVQFDAGRRRDPAAKAGLAELTVASLTRGVEAADGKPALNEAQILDGFADVAAQQQG 121 + VS+ G + + +A+LT A L RG + D + Q+ D FA + Sbjct: 538 VSVSLHLRFGDAQSLFGQRNVAQLTEAMLMRGTDKLDRR------QLADEFARLKIN--- 588 Query: 122 GAGQDRAGVSLRTLSSPAERDAALGLLARILAQPSFPQAALERDRALSIANLKEELTKPE 181 G R ++ A+ L+ + P A E+ + ++A L+ P+ Sbjct: 589 -------GSLYRFQTTRENLVPAIALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPD 641 Query: 182 AIAEKAFMQAAYGHHP-------YAIEASPATLQAITRDDLLAFHRSHYVANRAVIAMIG 234 A +A + + H+P +E A ++A+T + + FHR Y A++ +A++G Sbjct: 642 TRAGEA-LSVHFDHYPAGDWRAAQTLEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVG 700 Query: 235 DITPEQARTMAS----ALTRALPQGAALPALPPVPAPQGSEQRIAHPASQSHILIGAPA- 289 D P QAR+ T A P L +PA + P ++ + Sbjct: 701 DFDPAQARSAIDQAFGGWTSAAPYARVLQTWADIPA---KRLLVDTPDKENGVYFARQNI 757 Query: 290 -LQRGDPDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGF--SPLAQPGPFQIG 346 L DPD+ AL V N++LGG SRL D VR++ GLSY +YSG + L G I Sbjct: 758 RLSDTDPDYPALAVANFLLGGSSLKSRLADRVRQQDGLSYGIYSGLQVAALTDAGHLTIY 817 Query: 347 LQTKKEQTAEALRVTRATLDRFLQEGPTSAELKAAK--------------DNLTGGFALR 392 + + R + L++G + EL+ AK DN+ + L Sbjct: 818 AIAAPQNLDKVDAAVREEIALALKDGFSEEELQRAKSGILQQRNQQRAGNDNVAATWTLL 877 Query: 393 IDSNAKLLENLAVIGFYGLPLDYLDHWIERVRAVSVQDVRAAFGKHVHPQALSTVI 448 ++ + L + +D ++ ++++ V AA K+V P ++TVI Sbjct: 878 MNQDRNFL--------WQQEID------RKLASLTLAQVNAALRKYVDPARMTTVI 919 >gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum seropedicae SmR1] Length = 459 Score = 62.8 bits (151), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 67/261 (25%), Positives = 120/261 (45%), Gaps = 15/261 (5%) Query: 179 KPEAIAEKAFMQAAYGHHPY--AIEASPATLQAITRDDLLAFHRSHYVANRAVIAMIGDI 236 +P + +A AA+ HPY + LQ ++ D+ A++R Y N A + + GD+ Sbjct: 161 QPMGLLNEALNAAAWTAHPYHHPVVGWMDDLQHMSVQDIAAWYRQWYAPNNATLVVAGDV 220 Query: 237 TPEQARTMASALTRALPQGAALPALPPVPAPQGSEQR---IAHPASQSHILIG--APALQ 291 ++ +A +P LPA P PQ R + PA ++++ PAL+ Sbjct: 221 DAQRVLALARKYFGKIP-ARRLPAGKPQNEPQQLGMRRVTVKAPAENPYVVMAWKTPALR 279 Query: 292 R--GDPDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGFSPLAQPGPFQIGLQT 349 + D D +AL V + VL G +RL+ + G + +V + +S +A+ GP L+ Sbjct: 280 QVEQDQDVYALDVLSAVLDGYDN-ARLSASLVRTGGKATAVGASYSGVAR-GPVLFTLEG 337 Query: 350 KKEQ---TAEALRVTRATLDRFLQEGPTSAELKAAKDNLTGGFALRIDSNAKLLENLAVI 406 + TA+ + R ++R +EG + EL+ K L + DS + ++ Sbjct: 338 SPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQLIASQVYKRDSVFGQAMEIGMM 397 Query: 407 GFYGLPLDYLDHWIERVRAVS 427 GL +D IER+++V+ Sbjct: 398 ETAGLGQQNIDRIIERLKSVT 418 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,253,734 Number of extensions: 46965 Number of successful extensions: 212 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 209 Number of HSP's successfully gapped: 3 Length of query: 454 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 366 Effective length of database: 1,203,190 Effective search space: 440367540 Effective search space used: 440367540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)