BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4002.p (262 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 473 e-135 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 164 6e-42 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 126 2e-30 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 120 1e-28 gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 108 3e-25 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 473 bits (1218), Expect = e-135, Method: Compositional matrix adjust. Identities = 234/262 (89%), Positives = 251/262 (95%) Query: 1 MQEPLPHDDILVARHGEQVLHLTLNRPAVRNALRNQSLQEIVQELARAEQDDSVRVVVIS 60 MQ+ + ++D+L ARHGE VLHLTLNRPA+RNALRNQSL+EIV LARAE+DDSVRVVVIS Sbjct: 1 MQDTVAYEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVIS 60 Query: 61 GNDQAFAAGADLNEMIHKDAIATQLDVRAQYWRAIARFPKPLLAAVNGYALGAGCELLMH 120 GN++AFAAGADLNEMIHKDAIATQLDVRAQYWRAIARFPKP+LAAVNGYALGAGCELLMH Sbjct: 61 GNEKAFAAGADLNEMIHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMH 120 Query: 121 ADIAIAGRSARIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFITAEQALQAGL 180 ADIAIA R A+IGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFI+A+QALQAGL Sbjct: 121 ADIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGL 180 Query: 181 VAEVVEDEATLERSLALATRIAQKSPLAVRLAKEAMLQSFELGLEAGLLLERKSFSLMAA 240 VAEVV+D+ATLER+LALA IAQKSPLAVRLAKEAMLQSFELGLEAGLL ERKSFSLMAA Sbjct: 181 VAEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAA 240 Query: 241 SADRQEGIAAFQEKRAAVFSGR 262 SADRQEGIAAFQEKRAAVFSGR Sbjct: 241 SADRQEGIAAFQEKRAAVFSGR 262 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 164 bits (416), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 99/256 (38%), Positives = 155/256 (60%), Gaps = 2/256 (0%) Query: 5 LPHDDILVARHGEQVLHLTLNRPAVRNALRNQSLQEIVQELARAEQDDSVRVVVISGNDQ 64 + +++I V H E+V + L+RP NAL + + E+ + L + + + ++I+G+++ Sbjct: 1 MSYENIQVETH-EKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEK 59 Query: 65 AFAAGADLNEMIHKDAIAT-QLDVRAQYWRAIARFPKPLLAAVNGYALGAGCELLMHADI 123 AFAAGAD++ M D + + + + W + R KP++AAV GYALG GCEL M D Sbjct: 60 AFAAGADISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDF 119 Query: 124 AIAGRSARIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFITAEQALQAGLVAE 183 IA +A+ GQPEI +G +PGAGGTQRL R V K AM + L+G + AE+A +AGLV+ Sbjct: 120 IIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSR 179 Query: 184 VVEDEATLERSLALATRIAQKSPLAVRLAKEAMLQSFELGLEAGLLLERKSFSLMAASAD 243 +V + LE ++A A IA+ + K+++ +++E L G+ ER F A+ D Sbjct: 180 IVAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATED 239 Query: 244 RQEGIAAFQEKRAAVF 259 ++EG+ AF EKR VF Sbjct: 240 QKEGMKAFLEKRQPVF 255 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 86/247 (34%), Positives = 127/247 (51%), Gaps = 6/247 (2%) Query: 22 LTLNRPAVRNALRNQSLQEIVQELARAEQDDSVRVVVISGNDQAFAAGADLNEMIHK--- 78 +TLNRP N+ E+ +A + D SVRV++++G + F AG DL + K Sbjct: 17 ITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAGQDLGDRAVKPGD 76 Query: 79 DAIATQLDVRAQYW---RAIARFPKPLLAAVNGYALGAGCELLMHADIAIAGRSARIGQP 135 D + + Y +++ P P++ AVNG A GAG L + DI +A RSA + Sbjct: 77 DGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEV 136 Query: 136 EINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFITAEQALQAGLVAEVVEDEATLERSL 195 +G +P GGT L R VG AM M L GE I+AEQA + GL+ + V+D+ +E + Sbjct: 137 FCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKCVDDDQLMEEAH 196 Query: 196 ALATRIAQKSPLAVRLAKEAMLQSFELGLEAGLLLERKSFSLMAASADRQEGIAAFQEKR 255 A+A A+ + K + S L L LER + + S D +EG+ AF EKR Sbjct: 197 AMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRSRDYREGVTAFLEKR 256 Query: 256 AAVFSGR 262 A F+G+ Sbjct: 257 APQFTGQ 263 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 120 bits (301), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 86/238 (36%), Positives = 133/238 (55%), Gaps = 4/238 (1%) Query: 22 LTLNRPAVRNALRNQSLQEIVQELARAEQDDSVRVVVISG-NDQAFAAGADLNEMIHKD- 79 LTL+ P N +L ++ + + D + +V++G ++ F+AGADL D Sbjct: 18 LTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFSAGADLKLFADGDK 76 Query: 80 AIATQLDVR-AQYWRAIARFPKPLLAAVNGYALGAGCELLMHADIAIAGRSARIGQPEIN 138 A+A ++ R + + ++ F +AA+NGYA+G G E + DI IA A++ PE Sbjct: 77 AMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDIRIAEEQAQMALPEAA 136 Query: 139 VGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFITAEQALQAGLVAEVVEDEATLERSLALA 198 VG LP AGGTQ L VG+ A +M+L GE + AE AL+ GLV EVV ER+LALA Sbjct: 137 VGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEVVPRGQARERALALA 196 Query: 199 TRIAQKSPLAVRLAKEAMLQSFELGLEAGLLLERKSFSLMAASADRQEGIAAFQEKRA 256 + ++SP +V +K+ + + L L ER+ F + + D++EG+ AF EKR+ Sbjct: 197 QQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQDQREGVQAFLEKRS 254 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 108 bits (271), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 76/251 (30%), Positives = 127/251 (50%), Gaps = 2/251 (0%) Query: 9 DILVARHGEQVLHLTLNRPAVRNALRNQSLQEIVQELARAEQDDSVRVVVISGNDQAFAA 68 DI +++ + VL + +R +NA+ Q++ L AE D +VR ++ +G +AF+A Sbjct: 4 DIQLSK-ADGVLTIHFDREDKKNAITAAMYQQLADALKEAESDSAVRAILFTGKPKAFSA 62 Query: 69 GADLNEMIHKDAIATQLDVRAQYWRAIARFPKPLLAAVNGYALGAGCELLMHADIAIAGR 128 G DL + + K+ TQ Q+ I+ KP++AAV+G A+G G LL+H D+ A Sbjct: 63 GNDLEDFL-KNPPKTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAAD 121 Query: 129 SARIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFITAEQALQAGLVAEVVEDE 188 +A+ P +G P A + L G A + +L GE A++A Q G V V+ E Sbjct: 122 NAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRVMAQE 181 Query: 189 ATLERSLALATRIAQKSPLAVRLAKEAMLQSFELGLEAGLLLERKSFSLMAASADRQEGI 248 L + A ++A ++R+ K+ M ++ E ++ E + FS M + + +E Sbjct: 182 ELLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEAKEAF 241 Query: 249 AAFQEKRAAVF 259 AF EKR F Sbjct: 242 TAFFEKRKPDF 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 739,185 Number of extensions: 29322 Number of successful extensions: 135 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 122 Number of HSP's successfully gapped: 10 Length of query: 262 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 179 Effective length of database: 1,226,865 Effective search space: 219608835 Effective search space used: 219608835 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)