BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3998.p (512 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase... 767 0.0 gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase... 104 2e-23 gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase... 90 4e-19 >gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 512 Score = 767 bits (1981), Expect = 0.0, Method: Compositional matrix adjust. Identities = 376/512 (73%), Positives = 429/512 (83%) Query: 1 MNTHEHPHALPASATVAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAATKAIGDIRTSL 60 MN ++PHAL SA VAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAA KAIG I T+L Sbjct: 1 MNNRKYPHALSRSALVAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAAGKAIGAICTNL 60 Query: 61 DKLANKEKISAEVAQAASERLRVASSLQELRGARLVVEAIVEQLAAKQELFGKMEALVDA 120 DKL +K+K++ +AQAAS+R+ VA SL+EL A LV+EAIVEQLAAK ELFGK+EA+V Sbjct: 61 DKLVSKDKLTPALAQAASQRISVAQSLEELHQADLVIEAIVEQLAAKHELFGKLEAIVST 120 Query: 121 QCILASNTSSLSITHIGATLRHPERLVGMHFFNPVPQMALVEIIRGLASDATVAQCVYDT 180 +CILA+NTSSLSIT I A L+HPER+VGMHFFNPVP MALVEII GLASDA +AQC++DT Sbjct: 121 RCILATNTSSLSITQIAAGLQHPERVVGMHFFNPVPMMALVEIISGLASDAALAQCLHDT 180 Query: 181 AQAWGKLPVFASSTPGFIVNRLARPYYAEGLRVLHERGAQPATIDAIMRESGGFRMGPFE 240 A AWGK PV A STPGFIVNRLARPYYAEGLR+LHE GAQPATIDA+MRE+GGFRMGPFE Sbjct: 181 ALAWGKTPVMARSTPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVMREAGGFRMGPFE 240 Query: 241 LMDLIGHDVNFAVTQSVFQAYFNDPRFTPSLIQQELVHAGYLGRKSGRGFYRYGADAEPP 300 LMDLIGHDVNFAVTQSVF AYFNDPRFTPSLIQQELVHAGYLGRKSGRGFYRYG A P Sbjct: 241 LMDLIGHDVNFAVTQSVFHAYFNDPRFTPSLIQQELVHAGYLGRKSGRGFYRYGEAAGAP 300 Query: 301 QVVSLEPCSPPTVPLQLFGTHPLVEVIRARYKGRVEYHPPRADNLLIKAGPGRLYVTEGR 360 + + PT QL+G HPLVE+I RY G+V+ HP R D LL+K L +T+GR Sbjct: 301 RPETERDYPKPTATPQLYGDHPLVEMIHTRYPGQVQRHPARPDQLLMKVNAACLCITDGR 360 Query: 361 SATQQSYDYNMPDVVLVDLALDYAKATRLAITRADQCRDRAYEAVAGVLQNCGYALSPLA 420 SAT +++++ PD+VLVDLALDYA ATRLA+ +ADQCR + Y VAGVLQ CG LSP+ Sbjct: 361 SATLRAWEHQEPDLVLVDLALDYADATRLALAKADQCRPQRYAEVAGVLQACGLDLSPMQ 420 Query: 421 DVPGMVVMRTVCMLVNEAADAVNQGVCSVADADLAMQKGVNYPRGPLAWADALGTETVAS 480 DVPGM+VMRTVCMLVNEAADAVNQGVC+ ADAD+AM+KGVNYPRGPLAWA+ +G +TV Sbjct: 421 DVPGMMVMRTVCMLVNEAADAVNQGVCNAADADMAMRKGVNYPRGPLAWANEIGIDTVNQ 480 Query: 481 VLRQLSATYGEDRYRVSPLLQRLQASMRRFYE 512 VL L ATYGEDRYR+SPLLQRL AS R+F+E Sbjct: 481 VLHHLCATYGEDRYRISPLLQRLYASGRKFHE 512 >gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 805 Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 99/315 (31%), Positives = 149/315 (47%), Gaps = 28/315 (8%) Query: 16 VAVIGSGTMGAGIAQVAAVAGHPVLLYD------SREGAATKAIGDIRT-SLDKLANKEK 68 VAV+G+G MGA IA A PV+L+D + G +AI +++ S L NK+ Sbjct: 19 VAVLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSPAPLGNKD- 77 Query: 69 ISAEVAQAASERLRVASSLQELRGARLVVEAIVEQLAAKQELFGKMEALVDAQCILASNT 128 A + Q A+ L L G L++EAI E++ K +L+ K+ + + I ASNT Sbjct: 78 -DAALIQVANYE----DDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFASNT 132 Query: 129 SSLSITHI--GATLRHPERLVGMHFFNPVPQMALVEIIRGLASDATVA-QCVYDTAQAWG 185 S LSI + G R G+HFFNP M LVE+I +++ + Q G Sbjct: 133 SGLSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTTLG 192 Query: 186 KLPVFASSTPGFIVNRLARPYYAEGLRVLHERGAQPATIDAIM-RESGGFRMGPFELMDL 244 K V A TP F+ NR+ + + G +D + + G + G F D+ Sbjct: 193 KGVVRAFDTPNFVANRVGVFGILATIIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTADV 252 Query: 245 IGHDVNFAVTQSVFQAYFNDPRF----TPSLIQQELVHAGYLGRKSGRGFY-RYGADAEP 299 +G D V +++ +DP F TP L+ + LV G LG+KSG GFY + G D Sbjct: 253 VGLDTMGHVIRTMQDNLKDDPFFSSYATPPLLAK-LVEQGALGQKSGAGFYKKVGKD--- 308 Query: 300 PQVVSLEPCSPPTVP 314 ++ ++P VP Sbjct: 309 --ILRIDPAKGDYVP 321 >gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 323 Score = 90.1 bits (222), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 80/241 (33%), Positives = 110/241 (45%), Gaps = 15/241 (6%) Query: 14 ATVAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAATK---AIGDIRTSLDKLANKEKIS 70 A VAV+G+G +GA A G V D A K AI SL L E Sbjct: 16 ARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIARHWPSLQALGLAE--- 72 Query: 71 AEVAQAASERLRVASSLQE-LRGARLVVEAIVEQLAAKQELFGKMEALVDAQCILASNTS 129 A+ ERLR L+ L GA V E E+ K +LF +M+A + AQ +LAS++S Sbjct: 73 ----HASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQVVLASSSS 128 Query: 130 SLSITHIGATLRHPERLVGMHFFNPVPQMALVEIIRGLASDATVAQCVYDTAQAWGKLPV 189 L ++ + A RHP+R+V H FNP + LVE+I G + Q D A GK P+ Sbjct: 129 GLLMSKVQAACRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFYAAIGKKPI 188 Query: 190 FA-SSTPGFIVNRLARPYYAEGLRVLHERGAQPATIDAIMRESGGFR---MGPFELMDLI 245 G I NRL + E ++ + A ID + G R MGP+ + + Sbjct: 189 HVRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMGPYLNLHMS 248 Query: 246 G 246 G Sbjct: 249 G 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,532,783 Number of extensions: 62704 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 265 Number of HSP's successfully gapped: 3 Length of query: 512 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 423 Effective length of database: 1,198,455 Effective search space: 506946465 Effective search space used: 506946465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)