BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3997.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 486 e-139 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 137 6e-34 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 129 2e-31 gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 129 3e-31 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 126 2e-30 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 486 bits (1251), Expect = e-139, Method: Compositional matrix adjust. Identities = 236/263 (89%), Positives = 250/263 (95%) Query: 1 MSAPSILFENQDGVAVITLNRPDKLNSFTVAMHLELREAIAKLQADPGVRVLLLTGAGRG 60 MS+PSIL EN+DG+AVITLNRPDKLNSFTVAMHLELR+AIA LQADP VRVLLLTGAGRG Sbjct: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 Query: 61 FCAGQDLGDRAVTPGDEAVDLGHSIDQYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDLGDRAV PGD+ VDLG SID+YYGPLVKSLRALPFPVICAVNGVAAGAGANLPL Sbjct: 61 FCAGQDLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAEQWG 180 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAE+WG Sbjct: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 Query: 181 LIWKCIDDDQLMPQARAMAAQFAKAPTRGLAATKATLYASPANTLAQQLDLERDTMRELG 240 LIWKC+DDDQLM +A AMAA FA+APT+GLAATKATLYASPA+TL +QLDLERDTMR LG Sbjct: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 Query: 241 HSRDYREGVSAFLEKRAPHFTGQ 263 SRDYREGV+AFLEKRAP FTGQ Sbjct: 241 RSRDYREGVTAFLEKRAPQFTGQ 263 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 137 bits (346), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 91/262 (34%), Positives = 143/262 (54%), Gaps = 9/262 (3%) Query: 1 MSAPSILFENQDGVAVITLNRPDKLNSFTVAMHLELREAIAKLQADPGVRVLLLTGAGRG 60 MS +I E + V +I L+RP LN+ + + EL EA+ A + +++TG+ + Sbjct: 1 MSYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKA 60 Query: 61 FCAGQDLGDRAVTPGDEAVDL--GHSIDQYYGPLVKSLRALPFPVICAVNGVAAGAGANL 118 F AG D+ A G + +D+ G I + + ++LR + PVI AV G A G G L Sbjct: 61 FAAGADISAMA---GYDYMDVFKGEFITRNW----ETLRRIRKPVIAAVAGYALGGGCEL 113 Query: 119 PLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAEQ 178 + CD ++AA +A F + KLG++P GGT LPR V A+AM +AL G + AE+AE+ Sbjct: 114 AMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAER 173 Query: 179 WGLIWKCIDDDQLMPQARAMAAQFAKAPTRGLAATKATLYASPANTLAQQLDLERDTMRE 238 GL+ + + D+L+ +A A A A+ P + K ++ + TL++ + ER Sbjct: 174 AGLVSRIVAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYS 233 Query: 239 LGHSRDYREGVSAFLEKRAPHF 260 + D +EG+ AFLEKR P F Sbjct: 234 CFATEDQKEGMKAFLEKRQPVF 255 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 129 bits (324), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 91/260 (35%), Positives = 135/260 (51%), Gaps = 6/260 (2%) Query: 5 SILFENQDGVAVITLNRPDKLNSFTVAMHLELREAIAKLQADPGVRVLLLTGAG-RGFCA 63 ++ E AV+TL+ P N++T A +L + +L AD + L++TG G + F A Sbjct: 6 NLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFSA 64 Query: 64 GQDLGDRAVTPGDEAVDLGHSIDQYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACD 123 G DL + GD+A + + + +G ++L A I A+NG A G G LACD Sbjct: 65 GADL--KLFADGDKA--MAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACD 120 Query: 124 IVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAEQWGLIW 183 I +A A +GL+P GGT LP LVG A M L GE+++AE A + GL+ Sbjct: 121 IRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVE 180 Query: 184 KCIDDDQLMPQARAMAAQFAKAPTRGLAATKATLYASPANTLAQQLDLERDTMRELGHSR 243 + + Q +A A+A Q K +AA+K + + N L L ER+ +L ++ Sbjct: 181 EVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQ 240 Query: 244 DYREGVSAFLEKRAPHFTGQ 263 D REGV AFLEKR+P + Q Sbjct: 241 DQREGVQAFLEKRSPQWKNQ 260 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 129 bits (323), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 83/255 (32%), Positives = 128/255 (50%), Gaps = 7/255 (2%) Query: 6 ILFENQDGVAVITLNRPDKLNSFTVAMHLELREAIAKLQADPGVRVLLLTGAGRGFCAGQ 65 I DGV I +R DK N+ T AM+ +L +A+ + ++D VR +L TG + F AG Sbjct: 5 IQLSKADGVLTIHFDREDKKNAITAAMYQQLADALKEAESDSAVRAILFTGKPKAFSAGN 64 Query: 66 DLGDRAVTPGDEAVDLGHSIDQYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIV 125 DL D P + Q+ + + + P++ AV+GVA G G L L CD+V Sbjct: 65 DLEDFLKNPPKTQ---DAPVFQFLWQISHASK----PIVAAVSGVAVGIGTTLLLHCDLV 117 Query: 126 LAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAEQWGLIWKC 185 AA +A F F +LGL P+ + LP + G RA LLGE A++A Q G + + Sbjct: 118 YAADNAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRV 177 Query: 186 IDDDQLMPQARAMAAQFAKAPTRGLAATKATLYASPANTLAQQLDLERDTMRELGHSRDY 245 + ++L+P A+ AA+ A P + TK + + QQ+ E + + + + Sbjct: 178 MAQEELLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEA 237 Query: 246 REGVSAFLEKRAPHF 260 +E +AF EKR P F Sbjct: 238 KEAFTAFFEKRKPDF 252 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 86/247 (34%), Positives = 126/247 (51%), Gaps = 6/247 (2%) Query: 17 ITLNRPDKLNSFTVAMHLELREAIAKLQADPGVRVLLLTGAGRGFCAGQDLGDRAVTPGD 76 +TLNRP N+ E+ A+A+ + D VRV++++G + F AG DL + + D Sbjct: 22 LTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAFAAGADLNE--MIHKD 79 Query: 77 EAVDLGHSIDQYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEV 136 QY+ + + P P++ AVNG A GAG L + DI +AAR A + Sbjct: 80 AIATQLDVRAQYWRAIAR----FPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQP 135 Query: 137 FCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAEQWGLIWKCIDDDQLMPQAR 196 +G +P GGT L R VG AM M L GE ISA+QA Q GL+ + +DDD + + Sbjct: 136 EINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERTL 195 Query: 197 AMAAQFAKAPTRGLAATKATLYASPANTLAQQLDLERDTMRELGHSRDYREGVSAFLEKR 256 A+A A+ + K + S L L ER + + S D +EG++AF EKR Sbjct: 196 ALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEKR 255 Query: 257 APHFTGQ 263 A F+G+ Sbjct: 256 AAVFSGR 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 802,776 Number of extensions: 31490 Number of successful extensions: 115 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 106 Number of HSP's successfully gapped: 10 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)