BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3995.p (178 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313414|ref|YP_003777506.1| phenylacetic acid degradation p... 281 2e-77 >gi|300313414|ref|YP_003777506.1| phenylacetic acid degradation protein [Herbaspirillum seropedicae SmR1] Length = 181 Score = 281 bits (720), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 133/169 (78%), Positives = 145/169 (85%) Query: 10 EVLQAQIWAWLEAIPDPEIPVISVVDLGIVRAVEVSDEDAAAARCTVVITPTYSGCPAMG 69 + L+A+IW WLEA+PDPEIPV+SVVDLGIVR VE+ A ARCTVVITPTYSGCPAMG Sbjct: 13 DALKARIWDWLEAVPDPEIPVLSVVDLGIVREVEIEAAGAVDARCTVVITPTYSGCPAMG 72 Query: 70 VIAQDIGVALKQQGLAQVTIRTQLAPAWNTDWMSEKGRRNLKQYGIAPPAQQVIDISSIH 129 VIA+DI L+++GLAQV IR QLAPAW+TDWMSEKGR NL+QYGIAPPAQQVIDI I Sbjct: 73 VIAEDITTVLQEKGLAQVAIRIQLAPAWSTDWMSEKGRHNLRQYGIAPPAQQVIDIRGIQ 132 Query: 130 LKRHAAPQVPCPRCGSMHTVCVSQFGSTSCKALYQCKDCREPFDYFKAH 178 LK HAA QV CP CGS TVCVSQFGSTSCKALYQCKDCREPFDYFKAH Sbjct: 133 LKHHAAAQVDCPHCGSSRTVCVSQFGSTSCKALYQCKDCREPFDYFKAH 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.133 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 528,749 Number of extensions: 19008 Number of successful extensions: 57 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 57 Number of HSP's successfully gapped: 1 Length of query: 178 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 99 Effective length of database: 1,245,805 Effective search space: 123334695 Effective search space used: 123334695 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)