BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3988.p (686 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313407|ref|YP_003777499.1| ring-opening enzyme protein [He... 1250 0.0 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 103 7e-23 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 99 8e-22 gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 92 1e-19 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 92 2e-19 >gi|300313407|ref|YP_003777499.1| ring-opening enzyme protein [Herbaspirillum seropedicae SmR1] Length = 686 Score = 1250 bits (3234), Expect = 0.0, Method: Compositional matrix adjust. Identities = 629/684 (91%), Positives = 657/684 (96%) Query: 1 MTTRPPLLQSFIAGRWFGESPAQALHSAVNGHTVASTHAESIDFGEALDHARRQGLPGLM 60 MT PPLLQSFIAGRW G+ P+QALHSAVNG VASTH ESIDFGEAL+HAR+ GLP LM Sbjct: 1 MTAHPPLLQSFIAGRWLGQRPSQALHSAVNGSAVASTHEESIDFGEALEHARKTGLPALM 60 Query: 61 ALDFQQRAAILKALGKYLMEHKETLYAVSAHTGATRNDSWIDIEGGSGTLFAYASIGTNE 120 ALDFQQRAAILKAL KYLMEHKE LYAVSAHTGATR DSWIDIEGGSGTLF YASIG NE Sbjct: 61 ALDFQQRAAILKALAKYLMEHKEVLYAVSAHTGATRADSWIDIEGGSGTLFTYASIGANE 120 Query: 121 FPSSNLVHEGPALALGKQGRFSGTHILVPRGGVAVHINAFNFPIWGMLEKFAPSFLAGMP 180 FPSSNLVHEGPA+ LGKQGRF+GTHILVPRGGVAVHINAFNFP+WGMLEKFAP+FLAGMP Sbjct: 121 FPSSNLVHEGPAMRLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMP 180 Query: 181 CIVKPATATSYLTEAAVRLIQQSGLLPAGSLQLVIGATGDLLERLQAQDVVTFTGSADTA 240 CIVKPATATSYL EAAVRLIQQSGLLP GSLQLVIG+TGDLLERLQAQDVVTFTGSADTA Sbjct: 181 CIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLERLQAQDVVTFTGSADTA 240 Query: 241 AKLRAHPNLIANAIPFNAEADSLNCAILGPDVTADDEEFELFVKEVAREMSTKAGQKCTA 300 AKLRAHPNLIAN+IPFNAEADSLNCAILGPD+TADDEEF+LFVKEVAREM+TKAGQKCTA Sbjct: 241 AKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAREMTTKAGQKCTA 300 Query: 301 IRRAIVPRQHVDAVAERLAARLSKIVVGDPALESVRMGPLASKAQQRDVEERVTLLKQGA 360 IRRAIVPR+HVDAVAERLAARLSKIVVGDPALE+VRMGPLAS+AQQRDVEERV LL+ A Sbjct: 301 IRRAIVPRRHVDAVAERLAARLSKIVVGDPALETVRMGPLASRAQQRDVEERVALLRHSA 360 Query: 361 EVILGAGGAFQPQGEGVAAGAFFAPTLLLARDTGAGSPVHEVEAFGPVSTLMPYDDLDEA 420 ++ILGAG AF PQG+GV GAFFAPTLLLARDT AGSPVHEVEAFGPVSTLMPYDDLDEA Sbjct: 361 DLILGAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEAFGPVSTLMPYDDLDEA 420 Query: 421 LALAARGRGSLVASLVTRSPAVAARAIPVAAAWHGRLLVLDREAATESTGHGSPLPQLKH 480 LALAARGRGSLVASLVTR+PAVAARAIPVAAAWHGRLLVLDREAA ESTGHGSPLPQLKH Sbjct: 421 LALAARGRGSLVASLVTRTPAVAARAIPVAAAWHGRLLVLDREAAAESTGHGSPLPQLKH 480 Query: 481 GGPGRAGGGEELGGARAVKHYLQRTAVQGSPTMLSAVVGEHVRGAQVRDSGIHPFRHYFE 540 GGPGRAGGGEELGG+RAVKHYLQRTAVQGSPTML+AVVGEHVRGA+VR+SGIHPFRHYFE Sbjct: 481 GGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAVVGEHVRGARVRESGIHPFRHYFE 540 Query: 541 DLQIGDSHTTHRRTVTEADIVNFGCLSGDHFYMHFDDISARQSPFGKRIAHGYFVLSAAA 600 DLQIGDS TTHRRTVTEADIVNFGCLSGDHFYMHFD+I+ARQSPFGKRIAHGYFVLSAAA Sbjct: 541 DLQIGDSLTTHRRTVTEADIVNFGCLSGDHFYMHFDEIAARQSPFGKRIAHGYFVLSAAA 600 Query: 601 GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD 660 GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD Sbjct: 601 GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD 660 Query: 661 VEVSNQDGELVASYDILTLVAKKP 684 VEVSNQ+GELVASYDILTLVAKKP Sbjct: 661 VEVSNQEGELVASYDILTLVAKKP 684 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 103 bits (256), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 132/513 (25%), Positives = 216/513 (42%), Gaps = 62/513 (12%) Query: 11 FIAGRWFGESPAQALHSAVNGHTVASTHAESIDFGEALD-----HARRQGLPGLMALDFQ 65 +I G+W P Q A I G A D A R+ +P A Sbjct: 19 YIDGKW---QPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPL 75 Query: 66 QRAAILKALGKYLMEHKETLYAVSAHTGATRNDSWIDIEGGSGTLFAYASIGTNEFPSSN 125 +RAA+L + L+E E L+A + + +E G+ +YA + + Sbjct: 76 ERAALLGRVHALLLERSE-LFAQA-----------LTLEMGAP--ISYARTAHVPLAAEH 121 Query: 126 LVHEGPALALGKQGRFSGTHILVPRG-GVAVHINAFNFPIWGMLEKFAPSFLAGMPCIVK 184 L LA R GT +V GV I +N+PI+ + K P+ AG ++K Sbjct: 122 LRVARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLK 181 Query: 185 PATATSYLTEAAVRLIQQSGLLPAGSLQLVIGATGDLLERLQAQ---DVVTFTGSADTAA 241 P+ + ++ ++G+ PAG LV G+ ++ L A D+++ TGS Sbjct: 182 PSELSPLSALLFAEIVHEAGV-PAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGV 240 Query: 242 KLRAHPNLIANAIPFNAEADSLNCAILGPDVTADDEEFELFVKEVAREMSTKAGQKCTAI 301 L+A A + + P++ D + E + GQ C+A Sbjct: 241 -------LVAQAAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAP 293 Query: 302 RRAIVPRQHVDAVAERLAARLSKIVVGDPALESVRMGPLASKAQQRDVEERVTL-LKQGA 360 R IVPR + V E A L+++ VGDP + GPLA++AQ V++ + L+ GA Sbjct: 294 TRLIVPRAALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGA 353 Query: 361 EVILGAGGAFQPQGEGVAAGAFFAPTLLLARDTGAGSPVHEVEAFGPVSTLMPYDDLDEA 420 ++ AGG +P +G+A G + PT+ + + + E FGPV ++PYD ++EA Sbjct: 354 RLL--AGGPGRP--DGLAQGYYARPTIF--SEVHTDMVIAQEEIFGPVLAILPYDTVEEA 407 Query: 421 LALAARGRGSLVASLVTRSP----AVAARAIPVAAAWHGRLLVLDREAATESTGHGSPLP 476 +A+A L A + R AVAAR ++ + + P Sbjct: 408 IAIANDTVYGLGAHVQGRDKDQVRAVAARI----------------QSGQVHLNYPAWDP 451 Query: 477 QLKHGGPGRAGGGEELGGARAVKHYLQRTAVQG 509 Q GG ++G G E G ++ Y++ A+ G Sbjct: 452 QAPFGGYKQSGNGREY-GVEGMEEYMEVKAILG 483 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 99.4 bits (246), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 109/357 (30%), Positives = 155/357 (43%), Gaps = 34/357 (9%) Query: 152 GVAVHINAFNFPIWGMLEKFAPSFLAGMPCIVKPATATSYLTEAAVRLIQQSGLLPAGSL 211 GV HI +N+P+ + AG C+VKPA L + G P G+L Sbjct: 139 GVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSILRVAELAAEVGF-PEGAL 197 Query: 212 QLVIG---ATGDLLERLQAQDVVTFTGSADTAAKLRAHPNLIANAIPFNAEADSLNCAIL 268 +V G GDLL + + ++FTGS + L+ A + +L Sbjct: 198 NIVTGYGHEAGDLLVKHPDVNHISFTGSPNVG-------KLVVRAAAEHHTPVTLELGGK 250 Query: 269 GPDVTADDEEFELFVKEVAREMSTKAGQKCTAIRRAIVPRQHVDAVAERLAARLSKIVVG 328 P + +D + E + V + AGQ C+A R +V R + V ERL+ S++ VG Sbjct: 251 SPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLSGLFSQLQVG 310 Query: 329 DPALESVRMGPLASKAQQRDVEERVTLLKQGAEVILGAGGAFQPQGE---GVAAGAFF-A 384 ++ + GPL K QQ ERV A+ G A QG+ GV G F+ A Sbjct: 311 SSQMD-LDCGPLIRKTQQ----ERVAGFLSDAQR---DGIALAAQGKIAAGVPTGGFYQA 362 Query: 385 PTLLLARDTGAGSPVHEVEAFGPVSTLMPYDDLDEALALAARGRGSLVASLVTRSPAVAA 444 P LL RD + + E FGPV MP++D A+ALA LVAS+ TR Sbjct: 363 PALL--RDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRD---GG 417 Query: 445 RAIPVAAAWHGRLLVLDREAATESTGHGSPLP--QLKHGGPGRAGGGEELGGARAVK 499 R + +A A + ++ A G G LP +K G GR G E L G +K Sbjct: 418 RQMRLARAIRSGQVFINNYGA----GGGVELPFGGVKASGHGREKGFEALYGFTVLK 470 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 92.0 bits (227), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 106/357 (29%), Positives = 161/357 (45%), Gaps = 36/357 (10%) Query: 152 GVAVHINAFNFPIWGMLEKFAPSFLAGMPCIVKPATATSYLTEAAV-RLIQQSGLLPAGS 210 GV I+ FNFP+ L AP+ AG +VKP T + R+ +Q+GL PAG Sbjct: 147 GVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVSGGFMLARIFEQAGL-PAGL 205 Query: 211 LQLVIGA--TGDLLERLQAQDVVTFTGSADTAAKLRAHPNLIANAIPFNAEADSLNCAIL 268 L ++ G TG+ L ++ FTGS + I + + SL Sbjct: 206 LHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRR-------IGELAGRHLKKLSLELGGA 258 Query: 269 GPDVTADDEEFELFVKEVAREMSTKAGQKCTAIRRAIVPRQHVDAVAERLAARLSKIVVG 328 V +D + ++ A GQ C A R +V + + +RLA + + VG Sbjct: 259 NALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEGLLQRLADKARHLPVG 318 Query: 329 DPALESVRMGPLASKAQQRDVEERVT-LLKQGAEVILGAGGAFQPQGEGVAAGAFFAPTL 387 D A V +GP+ + Q + V V + GA+++ AGG ++ F+ PT+ Sbjct: 319 DGASGQVALGPMIDERQLQRVHALVQESVAAGAQLV--AGGRYEQ--------LFYQPTV 368 Query: 388 LLARDTGAGSPVHEVEAFGPVSTLMPYDDLDEALALAARGRGSLVASLVTRSPAVAARAI 447 L G V E E FGPV++++ +D+ ++A+ALA R G L A ++ SP+V RA+ Sbjct: 369 L--GGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVL--SPSV-GRAM 423 Query: 448 PVAAAW-HGRLLVLDREAATESTGHGSPLPQLKHGGPGRAGGGEELGGARAVKHYLQ 503 VAA G + V D+ E GGPG AG G +GG + Y Q Sbjct: 424 AVAARLRQGMVHVNDQTVNDECVN--------PFGGPGIAGNGASVGGPADWEEYTQ 472 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 91.7 bits (226), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 109/400 (27%), Positives = 158/400 (39%), Gaps = 47/400 (11%) Query: 56 LPGLMALDFQQRAAILKALGKYLMEHKETLYAVSAHTGAT--RNDSWIDIEGGSGTLFAY 113 LPG AL RA L AL + + H L + R +D+ + + Y Sbjct: 81 LPGWQALGGHGRARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHY 140 Query: 114 A---SIGTNEFPSSNLVHEGPALALGKQGRFSGTHILVPRGGVAVHINAFNFPIWGMLEK 170 A + +EFP H+ V GV I +NFP+ + K Sbjct: 141 AGWAQLQESEFPD---------------------HVPV---GVVGQIVPWNFPLLMLAWK 176 Query: 171 FAPSFLAGMPCIVKPATATSYLTEAAVRLIQQSGLLPAGSLQLVI--GATGDLLERLQAQ 228 AP+ G ++KPA TS L QQ+G LPAG L +V GATG + Sbjct: 177 IAPALALGNTVVLKPAENTSLTALLFAELAQQAG-LPAGVLNIVTGDGATGAAVVAHPGI 235 Query: 229 DVVTFTGSADTAAKLRAHPNLIANAIPFNAEADSLNCAILGPDVTADDEEFELFVKEVAR 288 + FTGS + LI + ++ +L P + +D + + ++ V Sbjct: 236 QKIAFTGSTEVG-------RLIREQTAGSGKSLTLELGGKSPFIVFEDADIDAAIEGVVD 288 Query: 289 EMSTKAGQKCTAIRRAIVPRQHVDAVAERLAARLSKIVVGDPALESVRMGPLASKAQQRD 348 + GQ C A R +V D RL R+ K+ VG P + MG L S Q Sbjct: 289 AIWFNQGQVCCAGSRLLVQEGIHDLFIARLKTRMQKLKVGAPLDKCSDMGALISPVQ--- 345 Query: 349 VEERVTLLKQGAEVILGAGGAFQPQGEGVAAGAFFAPTLLLARDTGAGSPVHEVEAFGPV 408 +E +L++QG + Q + G F+ PTLL A V E FGPV Sbjct: 346 LERVRSLVEQG---VREGAQCHQVVLDTPPGGCFYPPTLLTGVHPAA--TVASEEIFGPV 400 Query: 409 STLMPYDDLDEALALAARGRGSLVASLVTRSPAVAARAIP 448 M + DEA+ LA R L AS+ + + +A P Sbjct: 401 LVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALGVAP 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,144,882 Number of extensions: 94517 Number of successful extensions: 401 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 377 Number of HSP's successfully gapped: 20 Length of query: 686 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 594 Effective length of database: 1,184,250 Effective search space: 703444500 Effective search space used: 703444500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)