BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3945.p (145 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313336|ref|YP_003777428.1| large conductance mechanosensit... 246 5e-67 >gi|300313336|ref|YP_003777428.1| large conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] Length = 145 Score = 246 bits (628), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 140/145 (96%), Positives = 143/145 (98%) Query: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP Sbjct: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 Query: 61 LNGQAYGLPLAEAKKAGAVFAYGNFLTIFINFLILAFIIFQMVRAINKARDLATKNEEAA 120 LNGQAYGLPLAEA+KAGAVFAYGNFLTI INFLILAFIIFQMVRAINKARDLA+K+EEAA Sbjct: 61 LNGQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAA 120 Query: 121 PAAPAPTPEDVLLLREIRDSLKKQA 145 PAAPAPTPEDVLLLREIRDSLKKQ Sbjct: 121 PAAPAPTPEDVLLLREIRDSLKKQG 145 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.143 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 337,774 Number of extensions: 12040 Number of successful extensions: 59 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 59 Number of HSP's successfully gapped: 1 Length of query: 145 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 68 Effective length of database: 1,255,275 Effective search space: 85358700 Effective search space used: 85358700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)