BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3937.p (323 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 178 4e-46 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 163 2e-41 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 158 7e-40 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 108 6e-25 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 178 bits (452), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 113/314 (35%), Positives = 172/314 (54%), Gaps = 1/314 (0%) Query: 3 EYPTLQDIHQARERLLPHIRHTPLLRAEKIEKQLGCELYLKPETLQITGAFKIRGALNKS 62 E P+ D+ A ER+ P TP+LR+ + +LG +L+ K E Q GAFK RGA+N Sbjct: 9 ELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNAL 68 Query: 63 LSLPKEAIANGIIATSSGNHAQGLAYAARMLGVKALLVLPVTTPKIKIANTQALGAEVIL 122 +G+I SSGNHAQG A AA++LG+ A++V+P P K+A T+ GAEV+ Sbjct: 69 TQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVT 128 Query: 123 FDGDTAARWKRVAEIAEENHYVMVHAFEDPVVMAGQGTIGCEIIDDLPDVDTVIIPIGGG 182 +D T R A +A E ++ ++ P VMAGQGT E++ + +D + + +GGG Sbjct: 129 YDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGG 188 Query: 183 GLISGIATALKETRPSIRVVGAEPALTPKYYQSRINKQRTSLPLKNTIADGLRISVPGQN 242 GL+SG A A + P+ ++ G EP QS Q + TIADG + G+ Sbjct: 189 GLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKY 248 Query: 243 PYPIIEKYVDEIVLVEDEHIIEGMRILAKDAKLIAEPAASIGIGALLAGGLSVRPDEKVC 302 + +I + VD+I V DE ++ GMR A+ K++ EP +G A ++ ++V Sbjct: 249 TFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQ-GKRVG 307 Query: 303 AVLTGGNWDLFDLA 316 +++GGN DL LA Sbjct: 308 VIISGGNVDLGKLA 321 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 163 bits (412), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 91/271 (33%), Positives = 159/271 (58%), Gaps = 1/271 (0%) Query: 16 RLLPHIRHTPLLRAEKIEKQLGCELYLKPETLQITGAFKIRGALNKSLSLPKEAIANGII 75 R+ + TPL A + +++ +Y K E +Q +FK+RGA NK L + G+I Sbjct: 13 RVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQLKRGVI 72 Query: 76 ATSSGNHAQGLAYAARMLGVKALLVLPVTTPKIKIANTQALGAEVILFDGDTAARWKRVA 135 S+GNHAQG+A +A LG +A++V+P TTP++KI +A G EV+LF ++ Sbjct: 73 CASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHAL 132 Query: 136 EIAEENHYVMVHAFEDPVVMAGQGTIGCEIIDDLPD-VDTVIIPIGGGGLISGIATALKE 194 + ++ VH F+DP V+AGQGT+G EI+ PD + + + IGGGGLI+G+A+ +K Sbjct: 133 TLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKA 192 Query: 195 TRPSIRVVGAEPALTPKYYQSRINKQRTSLPLKNTIADGLRISVPGQNPYPIIEKYVDEI 254 RP I+++G + + +S +R +LP +DG + + G+ + I ++ VDE+ Sbjct: 193 VRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEV 252 Query: 255 VLVEDEHIIEGMRILAKDAKLIAEPAASIGI 285 ++V+ + + ++ + +D + I EPA ++ + Sbjct: 253 IIVDTDAVCTAIKDIFQDTRSIVEPAGALAV 283 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 158 bits (399), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 111/314 (35%), Positives = 178/314 (56%), Gaps = 18/314 (5%) Query: 6 TLQDIHQARERLLPHIRHTPLLRAEKIEKQLGCELYLKPETLQITGAFKIRGALNKSLSL 65 TL DI A RL I TP + + + + +G +++LK E LQ T +FK RGA NK + + Sbjct: 3 TLNDIEAAARRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLVDV 62 Query: 66 PKEAI-ANGIIATSSGNHAQGLAYAARMLGVKALLVLPVTTPKIKIANTQALGAEVILFD 124 A G++A S+GNHAQG+AY A+ LG+ A +V+P TP +KI T+ GA V+L Sbjct: 63 AASGGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVLHG 122 Query: 125 GDTAARWKRVAEIAEENHYVMVHAFEDPVVMAGQGTIGCEIIDDLPDVDTVIIPIGGGGL 184 A E+AE+ VH ++D ++AGQGT+ E++ +PD+D +++ IGGGGL Sbjct: 123 DTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGGGL 182 Query: 185 ISGIATALKETRPSIRVVGAEPALTPKYYQSRINKQR-TSLPL-KNTIADGLRISVPGQN 242 ++G+A A + +P I +VG + + + + +N + T P+ +TIA+G+ + PG Sbjct: 183 LAGMAVAARAIKPEIEIVGVQ----TRRFPTMVNAVKGTEHPVGASTIAEGIAVGTPGTL 238 Query: 243 PYPIIEKYVDEIVLVEDEHIIEGMRILAKDAKLIAEPAASIGIGALLAGGLSVR-PD--- 298 II + DE++LV++ I + + +L + K + E A + VR PD Sbjct: 239 TRAIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGA------AGLAALVRYPDRFR 292 Query: 299 -EKVCAVLTGGNWD 311 ++V VL+GGN D Sbjct: 293 GKRVGLVLSGGNID 306 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 108 bits (270), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 77/265 (29%), Positives = 130/265 (49%), Gaps = 2/265 (0%) Query: 5 PTLQDIHQARERLLPHIRHTPLLRAEKIEKQLGCELYLKPETLQITGAFKIRGALNKSLS 64 P+L DI QA + + T L + + LG E+++K E TGAFK+RG + Sbjct: 10 PSLTDIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLHH 69 Query: 65 LPKEAIA-NGIIATSSGNHAQGLAYAARMLGVKALLVLPVTTPKIKIANTQALGAEVILF 123 L ++ +G+++ + GNH Q + AAR LG+ A +V+P + K A +ALG E+I Sbjct: 70 LARQQPQLSGVVSATRGNHGQSVGLAARRLGISATIVVPQGNSREKNAAMRALGVELIEH 129 Query: 124 DGDTAARWKRVAEIAEENHYVMVHAFEDPVVMAGQGTIGCEIIDDLPDVDTVIIPIGGGG 183 + + ++A + M+ + +V AG + E+ P +D V++PIG G Sbjct: 130 GSEFQESREYAQQLAAQQQLHMIPSLHRDLV-AGVASYWMELFAAQPGLDVVLVPIGQGS 188 Query: 184 LISGIATALKETRPSIRVVGAEPALTPKYYQSRINKQRTSLPLKNTIADGLRISVPGQNP 243 + G A S R++G A Y S ++ P+ IADG+ VP Q Sbjct: 189 GMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIADGVACRVPDQAS 248 Query: 244 YPIIEKYVDEIVLVEDEHIIEGMRI 268 ++ VDE+V V D+ +++ M++ Sbjct: 249 LEVLFSEVDEVVAVTDDEVMDAMKL 273 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 930,395 Number of extensions: 35083 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 4 Length of query: 323 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 238 Effective length of database: 1,217,395 Effective search space: 289740010 Effective search space used: 289740010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)