BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3898.p (254 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehyd... 451 e-128 gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehyd... 245 2e-66 gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain)... 244 7e-66 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 106 2e-24 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 100 2e-22 >gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 451 bits (1160), Expect = e-128, Method: Compositional matrix adjust. Identities = 241/254 (94%), Positives = 246/254 (96%) Query: 1 MQLQGQAALVTGGASGLGAETVRQLVQAGARVSILDVNMEAAQALADELRCHAVRCDITD 60 MQLQGQAALVTGGASGLGAETVRQLVQAGARVSILDVNMEAAQALADELRCHAVRCDITD Sbjct: 1 MQLQGQAALVTGGASGLGAETVRQLVQAGARVSILDVNMEAAQALADELRCHAVRCDITD 60 Query: 61 SASVQAALDAAQEANGAPRILINCAGIGGAKRMVGKDGSAMPLEDFSRIVNVNLIGTFNV 120 S SV+ AL AAQ+ NGAPRILINCAGIGGAKRMVGKDG MPLEDFSRIVNVNLIGTFNV Sbjct: 61 SDSVETALQAAQQTNGAPRILINCAGIGGAKRMVGKDGKPMPLEDFSRIVNVNLIGTFNV 120 Query: 121 IRLVAARMAAAEPLAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQ 180 IRL AARMAAAE LAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQ Sbjct: 121 IRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQ 180 Query: 181 HGIRVVTIAPGLFLTPLLYKLPDEVQQSLAASIPFPKRLGKAEEYAQLALHIATNLSLNG 240 +GIRVVTIAPGLFLTPLLYKLP+EVQQSLA+SIPFPKRLGKAEEYAQLALHIATNLSLNG Sbjct: 181 YGIRVVTIAPGLFLTPLLYKLPEEVQQSLASSIPFPKRLGKAEEYAQLALHIATNLSLNG 240 Query: 241 EVIRLDGALRLAPR 254 EVIRLDGALRLAPR Sbjct: 241 EVIRLDGALRLAPR 254 >gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 245 bits (626), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 135/255 (52%), Positives = 181/255 (70%), Gaps = 4/255 (1%) Query: 1 MQLQGQAALVTGGASGLGAETVRQLVQAGARVSILDVNMEAAQALADELRCHAVRCDITD 60 M+++G ++TG ASGLGA T R + AG + + D+N EA + LA EL VRCD++ Sbjct: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSS 60 Query: 61 SASVQAALDAAQEANGAPRILINCAGIGGAKRMVGKDG-SAMPLEDFSRIVNVNLIGTFN 119 QA + AA EA G+ R L+NCAGI A+++VGK G M L F + +N+NL+G+FN Sbjct: 61 EEDAQAVVRAA-EALGSLRGLVNCAGIAPAEKLVGKSGPHTMAL--FMKTLNINLVGSFN 117 Query: 120 VIRLVAARMAAAEPLAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLA 179 + R A MA + + EGERGVI+ TASVAA+DGQ+GQ YAASKG I AMTLP+AR+L+ Sbjct: 118 MARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELS 177 Query: 180 QHGIRVVTIAPGLFLTPLLYKLPDEVQQSLAASIPFPKRLGKAEEYAQLALHIATNLSLN 239 + GIRV+TIAPG+F TP++ +P EV+ SL ++PFP RLG+A EYAQLA I N+ LN Sbjct: 178 RSGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLN 237 Query: 240 GEVIRLDGALRLAPR 254 GE IRLDGA+R+ P+ Sbjct: 238 GETIRLDGAIRMQPK 252 >gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain) dehydrogenase type II oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 244 bits (622), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 128/248 (51%), Positives = 174/248 (70%), Gaps = 4/248 (1%) Query: 9 LVTGGASGLGAETVRQLVQAGARVSILDVNMEAAQALADELRCHA--VRCDITDSASVQA 66 +VTG ASGLGA T QL++AGA+V + DVN A + + L +A V D+T S Q Sbjct: 9 VVTGAASGLGAATAEQLIRAGAKVVLADVNPVAGEKMQATLGANARFVLTDVTSETSAQE 68 Query: 67 ALDAAQEANGAPRILINCAGIGGAKRMVGKDGSAMPLEDFSRIVNVNLIGTFNVIRLVAA 126 A + GA L+NCAGI ++++GK G L+ F R +N+NL+G+FN+ RL A Sbjct: 69 VFGVAS-SMGAVVGLVNCAGIAPGEKLLGK-GGVHRLDSFMRALNINLVGSFNMARLAAQ 126 Query: 127 RMAAAEPLAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQHGIRVV 186 ++A A GERGVI+ TAS+AAFDGQ+GQ YAASKGG+ +MTLP+AR+LA+HGIRVV Sbjct: 127 HISAGPADATGERGVIIHTASIAAFDGQMGQVAYAASKGGVVSMTLPMARELARHGIRVV 186 Query: 187 TIAPGLFLTPLLYKLPDEVQQSLAASIPFPKRLGKAEEYAQLALHIATNLSLNGEVIRLD 246 TIAPG+ TP++ +P+EV +SL+ ++PFP RLGK E+A L +HI N LNGEVIR+D Sbjct: 187 TIAPGIIETPMMLGMPEEVLESLSRNVPFPPRLGKPSEFAGLVMHILENRYLNGEVIRMD 246 Query: 247 GALRLAPR 254 GALR++ + Sbjct: 247 GALRMSAK 254 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 106 bits (264), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 89/254 (35%), Positives = 127/254 (50%), Gaps = 24/254 (9%) Query: 3 LQGQAALVTGGASGLGAETVRQLVQAGARVSILDVNMEAAQALADELRCHAVRCDITDSA 62 L+G+ ALVTGG G+GA RQLV GARV + A + A V ++ Sbjct: 8 LKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHEEFAGS-EVTLVEMNVASEE 66 Query: 63 SVQAALDAAQEANGAPRILINCAGIGGAKRMVGKDGSAMPLEDFSRIVNVNLIGTFNVIR 122 SV+AAL + + I++NCAGI K G+ + E F +++ VNL GT V Sbjct: 67 SVRAALAGLTQLD----IVVNCAGII-------KRGAELETEVFEQVIAVNLTGTMRVC- 114 Query: 123 LVAARMAAAEPLAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQHG 182 AAA L RG IV TAS+ +F G GY+ASKGG+ +T LA A G Sbjct: 115 ------AAARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADG 168 Query: 183 IRVVTIAPGLFLTPLLYKLPDEVQQS--LAASIPFPKRLGKAEEYAQLALHIATNLS--L 238 IRV +APG TPL L D+ ++ + A P R G+ E+ Q+ + + + + Sbjct: 169 IRVNAVAPGWIATPLTQALQDDPVRAGPILARTPL-GRWGQPEDVGQVVAFLCSPAASFM 227 Query: 239 NGEVIRLDGALRLA 252 G ++ +DG +A Sbjct: 228 TGAIVPVDGGYLVA 241 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 99.8 bits (247), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 76/252 (30%), Positives = 125/252 (49%), Gaps = 20/252 (7%) Query: 6 QAALVTGGASGLGAETVRQLVQAGARVSILDVNMEAAQALADEL-----RCHAVRCDITD 60 + ALVTGGA G+GA +QL G V + D+N++AA AD L R ++ D+ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 61 SASVQAALDAAQEANGAPRILINCAGIGGAKRMVGKDGSAMPLEDFSRIVNVNLIGTFNV 120 S+ AA ++ G +L+N AG+ + PLE++ + +NVN+ G Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLD-----YPLENWLQTMNVNVTG---- 119 Query: 121 IRLVAARMAAAEPLAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQ 180 ++ A AA + + + G IV +S++ G+ Y SK + +T +A +LAQ Sbjct: 120 --VLLAGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQ 177 Query: 181 HGIRVVTIAPGLFLTPLLYKLPDE-VQQSLAASIPFPKRLGKAEEYAQLALHIATNLS-- 237 +GI V ++APG TP+ + E ++S +P +R G +E A ++A S Sbjct: 178 YGITVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPM-RRYGTTDEIASAVCYLAAEESAY 236 Query: 238 LNGEVIRLDGAL 249 + G VI +DG Sbjct: 237 ITGHVIPVDGGF 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 761,867 Number of extensions: 30262 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 45 Length of query: 254 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 171 Effective length of database: 1,226,865 Effective search space: 209793915 Effective search space used: 209793915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)