BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3895.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313293|ref|YP_003777385.1| enoyl-CoA hydratase/carnithine ... 483 e-138 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 91 1e-19 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 91 1e-19 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 84 1e-17 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 82 4e-17 >gi|300313293|ref|YP_003777385.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 259 Score = 483 bits (1242), Expect = e-138, Method: Compositional matrix adjust. Identities = 236/258 (91%), Positives = 245/258 (94%) Query: 1 MNQHSDLLDIQVHGAIAEIALNRSAKRNALNDPMVRAIRDCFEDLPPEVKVAIIHGRGEH 60 MNQHS+LLDIQ HGAIAEI LNR+AKRNALNDP+VRAIRDCFE+LP VKVAI+ G GEH Sbjct: 1 MNQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEH 60 Query: 61 FCAGLDLSELRERDTTESVHHSRMWHAAFEQISFGRVPVIAVLHGAVIGGGLELASAAHI 120 FCAGLDLSELRERDTTES+HHSRMWHAAFEQI+FGRVPVIAVLHGAVIGGGLELA+AAHI Sbjct: 61 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI 120 Query: 121 RVAEPSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLSAEEGHQVGISQY 180 RVAEPS FYGLPEGQRGLFVGGGGSVRISRLIGVARM DMMLTGRVLSAEEGHQ GI QY Sbjct: 121 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY 180 Query: 181 SVAEGAGLDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 240 SV GAG+DKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD Sbjct: 181 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 240 Query: 241 TKGRLAAFLDHKQNKVKP 258 TKGRLAAFLD KQNKVKP Sbjct: 241 TKGRLAAFLDLKQNKVKP 258 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 90.5 bits (223), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 81/260 (31%), Positives = 128/260 (49%), Gaps = 22/260 (8%) Query: 4 HSDLLDIQVHGA-IAEIALNRSAKRNALNDPMVRAIRDCF----EDLPPEVKVAIIHGRG 58 + DLL + HG + + LNR A RNAL + +R I ED V+V +I G Sbjct: 7 YEDLLAAR-HGEHVLHLTLNRPALRNALRNQSLREIVAALARAEED--DSVRVVVISGNE 63 Query: 59 EHFCAGLDLSELRERDT--TESVHHSRMWHAAFEQISFGRVPVIAVLHGAVIGGGLELAS 116 + F AG DL+E+ +D T+ ++ W A I+ P++A ++G +G G EL Sbjct: 64 KAFAAGADLNEMIHKDAIATQLDVRAQYWRA----IARFPKPILAAVNGYALGAGCELLM 119 Query: 117 AAHIRVAEPSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLSAEEGHQVG 176 A I +A A G PE G G GG+ R+ R +G M+L+G +SA++ Q G Sbjct: 120 HADIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAG 179 Query: 177 ISQYSVAEGAGLDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTE----SLMA 232 + V + A L++ LA IA+ +P+ + + + ++ GL+ E SLMA Sbjct: 180 LVAEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMA 239 Query: 233 AVAGSDPDTKGRLAAFLDHK 252 A A D + +AAF + + Sbjct: 240 ASA----DRQEGIAAFQEKR 255 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 90.5 bits (223), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 73/254 (28%), Positives = 120/254 (47%), Gaps = 3/254 (1%) Query: 8 LDIQVHGAIAEIALNRSAKRNALNDPMVRAIRDCF--EDLPPEVKVAIIHGRGEHFCAGL 65 + ++ H + I L+R NAL+D ++ + + D E+ II G + F AG Sbjct: 6 IQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAGA 65 Query: 66 DLSELRERDTTESVHHSRMWHAAFEQISFGRVPVIAVLHGAVIGGGLELASAAHIRVAEP 125 D+S + D + V +E + R PVIA + G +GGG ELA +A Sbjct: 66 DISAMAGYDYMD-VFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIAAD 124 Query: 126 SAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLSAEEGHQVGISQYSVAEG 185 +A +G PE + G+ G GG+ R+ R + A+ D+ LTGR++ AEE + G+ VA Sbjct: 125 NAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRIVAAD 184 Query: 186 AGLDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRL 245 L++A A IA V + R ++ ++ +G+ E + + D K + Sbjct: 185 KLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATEDQKEGM 244 Query: 246 AAFLDHKQNKVKPS 259 AFL+ +Q K S Sbjct: 245 KAFLEKRQPVFKHS 258 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 83.6 bits (205), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 71/272 (26%), Positives = 127/272 (46%), Gaps = 25/272 (9%) Query: 2 NQHSDLLDIQVHGAIAEIALNRSAKRNALNDPMVRAIRDCFEDLPPE--VKVAIIHGRGE 59 + H D+L+ + I + LNR NAL++ M+ A++ +D+ + V+ I+ G+ Sbjct: 3 DHHPDILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGK 62 Query: 60 HFCAGLDLSELRERDTTESVHHSRMWHAAFEQISFGR---VPVIAVLHGAVIGGGLELAS 116 FCAG DL +++ T + ++ ++H + R VPVIA + G G +L + Sbjct: 63 AFCAGHDLRQMQA--TPQQTYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVA 120 Query: 117 AAHIRVAEPSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLSAEEGHQVG 176 + +A SA + + GLF +V ++R + + R +M++TG+ + A G Sbjct: 121 TCDLAIAAESARFAVSGINVGLFC-STPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWG 179 Query: 177 ISQYSVAE----GAGLDKARELAER----IARNAPMTNYGVMHVLPRIHDQSIQDGLMTE 228 + +V E A ARE+ + IAR M M LP + + + +M Sbjct: 180 LINQAVPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAE--VMAC 237 Query: 229 SLMAAVAGSDPDTKGRLAAFLDH-KQNKVKPS 259 ++M DP+ + + AFLD K N P+ Sbjct: 238 NIM------DPEAQEGITAFLDKRKPNWAAPT 263 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 82.0 bits (201), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 65/232 (28%), Positives = 108/232 (46%), Gaps = 4/232 (1%) Query: 27 RNALNDPMVRAIRDCFEDLPPEVKVAIIHGRGEHF-CAGLDLSELRERDTTESVHHSRMW 85 R AL D + R +++ D ++ ++ G GE F AG DL + D + +R + Sbjct: 30 RAALAD-LTRLVKELNAD--RDIYTLVVTGEGEKFFSAGADLKLFADGDKAMAREMARRF 86 Query: 86 HAAFEQISFGRVPVIAVLHGAVIGGGLELASAAHIRVAEPSAFYGLPEGQRGLFVGGGGS 145 AFE +S R IA ++G +GGGLE A A IR+AE A LPE GL GG+ Sbjct: 87 GEAFETLSAFRGVSIAAINGYAMGGGLECALACDIRIAEEQAQMALPEAAVGLLPCAGGT 146 Query: 146 VRISRLIGVARMADMMLTGRVLSAEEGHQVGISQYSVAEGAGLDKARELAERIARNAPMT 205 + L+G M+L G ++AE ++G+ + V G ++A LA++ + +P + Sbjct: 147 QNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEVVPRGQARERALALAQQAEKQSPSS 206 Query: 206 NYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRLAAFLDHKQNKVK 257 ++ + L TE + D + + AFL+ + + K Sbjct: 207 VAASKQLIQGARHNPLGTILQTERELFVDLFDTQDQREGVQAFLEKRSPQWK 258 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 842,967 Number of extensions: 36968 Number of successful extensions: 150 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 9 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)