BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3884.p (314 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 229 2e-61 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 184 8e-48 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 174 7e-45 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 73 3e-14 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 229 bits (585), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 138/318 (43%), Positives = 183/318 (57%), Gaps = 4/318 (1%) Query: 1 MNVIIDSITQDTADIRVFLLKSADGSPLPTYKAGAHIDLTLGNGLVRQYSLCCRNPSGEN 60 M +I+D++T +T DIR F L ADG+PLP Y+ GAHID+T G++RQYSLC Sbjct: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 Query: 61 YRIAVKRETNSRGGSSWLHEHAHPGTPLQAGIPRSAFGLTDAPGPHLLLAGGIGITPLLA 120 Y IAVKRE+ SRGGS+ LH+ G L+ G PR+ F L + HLL GIG+TPLLA Sbjct: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Query: 121 MAYALLADGRAFTLTCFARDESMLPLRNELHEGPLAIHTRIFCGLDEVATRTGIAEVM-A 179 MAY L A+ + FTL F R + L P A H R + + + A Sbjct: 121 MAYRLWAEEKRFTLHYFVRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEA 180 Query: 180 AHAQAQVYTCGPPAFMVAVGELASARYGADHFHQESFQ--PAAASAAGTDTPFMIRI-ND 236 +A VYTCGP FM AV + ASA + H E FQ PAA +AAG D F +++ + Sbjct: 181 GSKEAHVYTCGPAPFMDAVVKCASATRSEEAIHLEHFQAAPAAPAAAGGDGAFEVKLASS 240 Query: 237 GREITVPAGQSALASLQEAGIDLACSCEVGVCGTCKVAVRQGQPDHRDSVLSAEEKACGK 296 G+ + +P G + L GI + SC G+CGTC V + +G+PDHRD+ LS +EKA Sbjct: 241 GKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAND 300 Query: 297 WFMPCVSRSTSDVLVLDI 314 CVSR+ S +LVLD+ Sbjct: 301 QICTCVSRARSGMLVLDL 318 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 184 bits (467), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 122/318 (38%), Positives = 168/318 (52%), Gaps = 7/318 (2%) Query: 1 MNVIIDSITQDTADIRVFLLKSADGSPLPTYKAGAHIDLTLGNGLVRQYSLCCRNPSGEN 60 + V + + ++ DI L + DG+ LP + AGAHID+ L GLVRQYSLC Sbjct: 4 LQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQHR 63 Query: 61 YRIAVKRETNSRGGSSWLHEHAHPGTPLQAGIPRSAFGLTDAPGPH-LLLAGGIGITPLL 119 Y+I V R+ NSRGGS +HE G L PR+ F L P H LLLAGGIG+TP+L Sbjct: 64 YQIGVLRDVNSRGGSVAVHEALKLGDILTISAPRNQFALV--PAQHSLLLAGGIGVTPIL 121 Query: 120 AMAYALLADGRAFTLTCFARDESMLPLRNELHEGPLAIHTRIFCGLDEVATRTGIAEVMA 179 MA AL A G +F + AR + R + A E A + + ++A Sbjct: 122 CMAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALLA 181 Query: 180 -AHAQAQVYTCGPPAFMVAVGELASAR-YGADHFHQESFQPAAASAAGTDTPFMIRI-ND 236 A +Y CGP F+ V + A AR + H E F A A G F +++ + Sbjct: 182 QADPATHLYVCGPAGFIAHVVDTARARGWPEAQVHFEYFGAAPVKADGNGA-FEVKLASS 240 Query: 237 GREITVPAGQSALASLQEAGIDLACSCEVGVCGTCKVAVRQGQPDHRDSVLSAEEKACGK 296 G+ T+P ++ + L E G+D+ SCE G+CGTC V +G+PDHRD L+ EE+A Sbjct: 241 GQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAND 300 Query: 297 WFMPCVSRSTSDVLVLDI 314 F PC SR+ S +LVLD+ Sbjct: 301 QFTPCCSRARSALLVLDL 318 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 174 bits (442), Expect = 7e-45, Method: Compositional matrix adjust. Identities = 128/322 (39%), Positives = 169/322 (52%), Gaps = 14/322 (4%) Query: 5 IDSITQDTADIRVFLLKSADGSPLPTYKAGAHIDLTLGNGLVRQYSLCCRNPSGENYRIA 64 + ++ Q DIR+ L S DG+ LP + GAH+ L L +GLVR YSLC + Y IA Sbjct: 20 VRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQRDCYLIA 79 Query: 65 VKRETNSRGGSSWLHEHAHPGTPLQAGIPRSAFGLTDAPGPHLLLAGGIGITPLLAMAYA 124 VKRE +SRGGS++LHE GT + A P +AF L LLLA GIGITPL AMA Sbjct: 80 VKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPLHAMAAE 139 Query: 125 LLADGRAFTLTCFARDESMLPLRNELHEGPLAIHTRIFCGLDEVATRTGIAEVMAAHAQA 184 L GR L FAR + +EL G + + GLD T IA +AA ++ Sbjct: 140 LATQGRRHRLHYFARSLAHAAFFHELAAGSQGMSLHL--GLDGAETEHAIAAALAAAPRS 197 Query: 185 Q-VYTCGPPAFMVAV---GELASARYGADHFHQESFQPAAASAAGTDTPF--------MI 232 +Y CGP F+ A +LA R HF + + A+ A P ++ Sbjct: 198 SPLYVCGPSPFIDAARRHAQLAGWREQDIHFERFAAPAMPAAEADPARPVQAPDSTFELV 257 Query: 233 RINDGREITVPAGQSALASLQEAGIDLACSCEVGVCGTCKVAVRQGQPDHRDSVLSAEEK 292 G V GQS +A+ + G+ + SC G CG+C+ V +GQP HRDSVLSA E+ Sbjct: 258 LQRSGLRCQVLPGQSIVAAAAQVGVVIGTSCGEGFCGSCESTVLEGQPWHRDSVLSAAER 317 Query: 293 ACGKWFMPCVSRSTSDVLVLDI 314 A G+ +PCVSR LVLD+ Sbjct: 318 ASGRRILPCVSRCAGTRLVLDL 339 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 73.2 bits (178), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 72/244 (29%), Positives = 107/244 (43%), Gaps = 29/244 (11%) Query: 2 NVIIDSITQDTADIRVFLLKSADGSPLPTYKAGAHIDLTL---GNGLVRQYSLCCR-NPS 57 N I+ ++ +I F DG L + G +I L + G RQYSL + Sbjct: 157 NFIVSRKVAESEEITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQA 216 Query: 58 GE--NYRIAVKRETNSRGGSSWLHEHAHPGTPLQAGIPRSAFGLTDAPGPHLLLAGGIGI 115 G+ YRI+VKRE + S LH+ H G+ ++ P F L + P L++GG+GI Sbjct: 217 GQPPQYRISVKREPGGKV-SRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGVGI 275 Query: 116 TPLLAMAYALLADGRAFTLTCFARDESMLPLRNELHEGPLAIHTRI---FC--------- 163 TP+++M A L GR AR + R +L E A H ++ FC Sbjct: 276 TPMMSMLAAALPSGRPIHFIHAARHGGVHAFRRQLEE-LAARHPQLQLRFCYEQPRQGDA 334 Query: 164 -----GLDEVATRTGIAEVMAAHAQAQVYTCGPPAFMVAVG-ELASARYGADHFHQESFQ 217 GL E R+ + + + + Y GP +FM A+ +LA A E F Sbjct: 335 APHATGLIE---RSLLQQWLPGNPDLDAYFVGPKSFMKAIKRQLAELGVPAAQSRYEFFG 391 Query: 218 PAAA 221 PAA+ Sbjct: 392 PAAS 395 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,055,387 Number of extensions: 46847 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 4 Length of query: 314 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 229 Effective length of database: 1,217,395 Effective search space: 278783455 Effective search space used: 278783455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)