BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3878.p (318 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 573 e-165 gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 234 5e-63 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 211 7e-56 gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 89 5e-19 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 66 3e-12 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 573 bits (1476), Expect = e-165, Method: Compositional matrix adjust. Identities = 286/318 (89%), Positives = 299/318 (94%) Query: 1 MTTLQVKVAARREEAQDIVALELVAADGQPLPPFTAGAHIDVHLPGGLVRQYSLCNAPHE 60 MTTLQVKVAARREEA DI+ALELVA DG LP FTAGAHIDVHLPGGLVRQYSLCNAPHE Sbjct: 1 MTTLQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHE 60 Query: 61 QHRYQIGVLRDANSRGGSVAVHEALQVGELITISAPRNQFALHSARHSLLLAGGIGITPI 120 QHRYQIGVLRD NSRGGSVAVHEAL++G+++TISAPRNQFAL A+HSLLLAGGIG+TPI Sbjct: 61 QHRYQIGVLRDVNSRGGSVAVHEALKLGDILTISAPRNQFALVPAQHSLLLAGGIGVTPI 120 Query: 121 LCMAEALAAEGASFEMHYCARSPARQAFRERIAACGFADRVVHHYDDGAAAQKLDLAALL 180 LCMAEALAA GASFEMHYCARSPARQAFRERIAA GFA RVVHHYDDG AAQKLDL ALL Sbjct: 121 LCMAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALL 180 Query: 181 ARVDPATHLYVCGPTGFIAHVVDTARARGWPDSQVHYEYFGAAPVNVDGNGAFEVKLASS 240 A+ DPATHLYVCGP GFIAHVVDTARARGWP++QVH+EYFGAAPV DGNGAFEVKLASS Sbjct: 181 AQADPATHLYVCGPAGFIAHVVDTARARGWPEAQVHFEYFGAAPVKADGNGAFEVKLASS 240 Query: 241 GQVYTIPPERTVIQVLVEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAND 300 GQVYTIPPERTVIQVL EHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAND Sbjct: 241 GQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAND 300 Query: 301 QFTPCCSRARSPMLVLDL 318 QFTPCCSRARS +LVLDL Sbjct: 301 QFTPCCSRARSALLVLDL 318 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 234 bits (598), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 142/319 (44%), Positives = 185/319 (57%), Gaps = 5/319 (1%) Query: 4 LQVKVAARREEAQDIVALELVAADGQPLPPFTAGAHIDVHLPGGLVRQYSLCNAPHEQHR 63 +++ V +E DI + LV ADG PLP + GAHIDV P G++RQYSLC ++ Sbjct: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 Query: 64 YQIGVLRDANSRGGSVAVHEALQVGELITISAPRNQFALH-SARHSLLLAGGIGITPILC 122 Y I V R++ SRGGS A+H+ ++ G + + PRN FAL SA LL GIG+TP+L Sbjct: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Query: 123 MAEALAAEGASFEMHYCARSPARQAFRERIAACGFADRVVHHYDDGAAAQKLDLAALLAR 182 MA L AE F +HY R+ AF + + FA V HY A + L L Sbjct: 121 MAYRLWAEEKRFTLHYFVRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEA 180 Query: 183 VDPATHLYVCGPTGFIAHVVDTARARGWPDSQVHYEYF---GAAPVNVDGNGAFEVKLAS 239 H+Y CGP F+ VV A A + +H E+F AAP G+GAFEVKLAS Sbjct: 181 GSKEAHVYTCGPAPFMDAVVKCASAT-RSEEAIHLEHFQAAPAAPAAAGGDGAFEVKLAS 239 Query: 240 SGQVYTIPPERTVIQVLVEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAN 299 SG+V IP ++ +L G+ + SC +GICGTC+ +LEGEPDHRD L+ +E+AAN Sbjct: 240 SGKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAN 299 Query: 300 DQFTPCCSRARSPMLVLDL 318 DQ C SRARS MLVLDL Sbjct: 300 DQICTCVSRARSGMLVLDL 318 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 211 bits (536), Expect = 7e-56, Method: Compositional matrix adjust. Identities = 142/331 (42%), Positives = 184/331 (55%), Gaps = 19/331 (5%) Query: 2 TTLQVKVAARREEAQDIVALELVAADGQPLPPFTAGAHIDVHLPGGLVRQYSLCNAPHEQ 61 TTL ++V A R+ AQDI LELV+ DG LPP+T GAH+ + L GLVR YSLCN P ++ Sbjct: 14 TTLMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQR 73 Query: 62 HRYQIGVLRDANSRGGSVAVHEALQVGELITISAPRNQFALHS-ARHSLLLAGGIGITPI 120 Y I V R+ +SRGGS +HE +Q G I+ S P N F L + R LLLA GIGITP+ Sbjct: 74 DCYLIAVKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPL 133 Query: 121 LCMAEALAAEGASFEMHYCARSPARQAFRERIAACGFADRVVHHYDDGAAAQKLDLAALL 180 MA LA +G +HY ARS A AF +AA G +H DGA + AAL Sbjct: 134 HAMAAELATQGRRHRLHYFARSLAHAAFFHELAA-GSQGMSLHLGLDGAETEHAIAAALA 192 Query: 181 A--RVDPATHLYVCGPTGFIAHVVDTARARGWPDSQVHYEYFGAA-----------PVNV 227 A R P LYVCGP+ FI A+ GW + +H+E F A PV Sbjct: 193 AAPRSSP---LYVCGPSPFIDAARRHAQLAGWREQDIHFERFAAPAMPAAEADPARPVQA 249 Query: 228 DGNGAFEVKLASSGQVYTIPPERTVIQVLVEHGVDVPVSCEQGICGTCLTRVLEGEPDHR 287 + FE+ L SG + P ++++ + GV + SC +G CG+C + VLEG+P HR Sbjct: 250 P-DSTFELVLQRSGLRCQVLPGQSIVAAAAQVGVVIGTSCGEGFCGSCESTVLEGQPWHR 308 Query: 288 DQYLTDEERAANDQFTPCCSRARSPMLVLDL 318 D L+ ERA+ + PC SR LVLDL Sbjct: 309 DSVLSAAERASGRRILPCVSRCAGTRLVLDL 339 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 89.0 bits (219), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 88/348 (25%), Positives = 153/348 (43%), Gaps = 49/348 (14%) Query: 12 REEAQDIVALEL-VAADGQPLPPFTAGAHIDV--HLPG-GLVRQYSLCNAPHEQHRYQIG 67 ++E +D + + V A+ Q + G H+ + + G L R YS+C+A E+ Sbjct: 13 KQETRDTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSICSAVQERQ----- 67 Query: 68 VLRDANSR--GG--SVAVHEALQVGELITISAPRNQFAL----HSARHSLLLAGGIGITP 119 LR A R GG S +E+ G+ I + P F + + +H L A G GITP Sbjct: 68 -LRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAAGSGITP 126 Query: 120 ILCMAEA--LAAEGASFEMHYCARSPARQAFRERIAACGFADRVVHHYD--DGAAAQKLD 175 ++ + + L+ + F + Y R+ + F+E + D + ++ + ++ D Sbjct: 127 MMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELT--DLKDAYLERFNVVYVMSREQQD 184 Query: 176 LAALLARVDPAT---------------HLYVCGPTGFIAHVVDTARARGWPDSQVHYEYF 220 + R+D A ++CGP + V + +A G P +Q+ E F Sbjct: 185 VELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPEEMVQAVAASLQAHGLPKTQIKTELF 244 Query: 221 GA-------APVNVDGNGAFEVKLASSG--QVYTIPPER-TVIQVLVEHGVDVPVSCEQG 270 A A V G EV + G V+T+ E+ +V++ ++HG+D+ SC+ G Sbjct: 245 AASTPPRAHAARTVVGKQECEVTVIVDGYHNVFTMDKEKESVLEAGLKHGIDLRYSCKGG 304 Query: 271 ICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSRARSPMLVLDL 318 +C TC +V+EG+ D Y ++ A C S S L+LD Sbjct: 305 VCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDF 352 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 67/240 (27%), Positives = 105/240 (43%), Gaps = 30/240 (12%) Query: 8 VAARREEAQDIVALELVAADGQPLPPFTAGAHIDVHLPGGLV------RQYSLC---NAP 58 V+ + E+++I + V DG L F G +I + + +V RQYSL Sbjct: 160 VSRKVAESEEITSFHFVPQDGGELLDFAPGQYIGLRM---VVDGEEQRRQYSLSALLRQA 216 Query: 59 HEQHRYQIGVLRDANSRGGSVA--VHEALQVGELITISAPRNQFALHSARHSL-LLAGGI 115 + +Y+I V R+ GG V+ +H+ + VG ++ + P +F L ++ L L++GG+ Sbjct: 217 GQPPQYRISVKREP---GGKVSRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGV 273 Query: 116 GITPILCMAEALAAEGASFEMHYCARSPARQAFR---ERIAA------CGFADRVVHHYD 166 GITP++ M A G + AR AFR E +AA F D Sbjct: 274 GITPMMSMLAAALPSGRPIHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPRQGD 333 Query: 167 DGAAAQKLDLAALLARV---DPATHLYVCGPTGFIAHVVDTARARGWPDSQVHYEYFGAA 223 A L +LL + +P Y GP F+ + G P +Q YE+FG A Sbjct: 334 AAPHATGLIERSLLQQWLPGNPDLDAYFVGPKSFMKAIKRQLAELGVPAAQSRYEFFGPA 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 971,564 Number of extensions: 39511 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 6 Length of query: 318 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 233 Effective length of database: 1,217,395 Effective search space: 283653035 Effective search space used: 283653035 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)