BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3871.p (316 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydro... 583 e-168 gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydr... 129 2e-31 gi|300313799|ref|YP_003777891.1| arylesterase [Herbaspirillum se... 75 9e-15 >gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 583 bits (1502), Expect = e-168, Method: Compositional matrix adjust. Identities = 286/316 (90%), Positives = 297/316 (93%) Query: 1 MIQAAVYAGVPAGNTAFTHAQKILREVGEQIGYVVAPQAPTDSVHPGVGREGRTASSPAL 60 MIQAAVYAGVPAGNTAFTHAQKILREVGEQIGY VAPQAP SVHPGVGREGRTASSPAL Sbjct: 1 MIQAAVYAGVPAGNTAFTHAQKILREVGEQIGYAVAPQAPAASVHPGVGREGRTASSPAL 60 Query: 61 HYTLREPRNGKAPRHTVVLSHALGTDLMMWDGLANLLAADCRVITYDHRGHGSSEKVDGL 120 HYT+REPRNGKAPRHTVVLSHALGTDLMMWDGLAN LAADCRVI YDHRGHGSSEK DGL Sbjct: 61 HYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGL 120 Query: 121 YSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPALVRALVLANTTSGYPQA 180 YSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHP LVRALVLANTTS YP A Sbjct: 121 YSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDA 180 Query: 181 ARDAWQQRIATVRAEGIEAIADAVMGRYFHEDFRAAQAATVARYRHRLVATDAVGYVGCC 240 AR+AWQQRIATVRAEGIE IADAVMGRYFHE FR+AQAATVARYR RLV TDAVGYVGCC Sbjct: 181 AREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCC 240 Query: 241 HAVGTVDTAARLGEIKLPALVIAGELDQGTPVSMAQVLVEGIAGARLEVIAGASHISAVE 300 HAVGTVDTAARLG I++P LVIAGELDQGTPV+MAQ LV+GI+GARL VIAGASH+SAVE Sbjct: 241 HAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVE 300 Query: 301 QPGAFAELVCGFIEGL 316 QP FAELVCGFI+G+ Sbjct: 301 QPALFAELVCGFIDGI 316 >gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydrolase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 129 bits (325), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 101/256 (39%), Positives = 127/256 (49%), Gaps = 7/256 (2%) Query: 60 LHYTLREPRNGKAPRHTVVLSHALGTDLMMWDGLANLLAADCRVITYDHRGHGSSEKVDG 119 LHY L G A +VLS++LGT L MWD RV+ YD RGHG SE G Sbjct: 11 LHYQLE----GDASLPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQSEVTPG 66 Query: 120 LYSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPALVRALVLANTTSGYPQ 179 S+A L D LL L + GLSMGG LA+ HP + L+L NT G Sbjct: 67 ECSIAQLGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRINKLILCNT--GAKI 124 Query: 180 AARDAWQQRIATVRAEGIEAIADAVMGRYFHEDFRAAQAATVARYRHRLVATDAVGYVGC 239 +AW RI TVR EG+ AIA AV+ R+ ++ A V L+AT A GY Sbjct: 125 GTAEAWNSRIETVRREGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEGYAAA 184 Query: 240 CHAVGTVDTAARLGEIKLPALVIAGELDQGTPVSMAQVLVEGIAGARLEVIAGASHISAV 299 C AV D + I+ P L+IAG D TP + AQ + I GA+ V A+HIS Sbjct: 185 CAAVRDHDLREAIAGIRAPTLIIAGSGDVPTPPADAQFMRATIPGAQY-VEFDAAHISNQ 243 Query: 300 EQPGAFAELVCGFIEG 315 +Q AF + V F+ G Sbjct: 244 QQAAAFTQAVVQFLTG 259 >gi|300313799|ref|YP_003777891.1| arylesterase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 74.7 bits (182), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 72/254 (28%), Positives = 106/254 (41%), Gaps = 16/254 (6%) Query: 77 VVLSHALGTDLMMWDGLANLLAA-DCRVITYDHRGHGSSEKVDGLYSMADLADDAARLLR 135 VV H D MW+ N LA RVI YD RG G S + Y ADD A L+ Sbjct: 23 VVFGHGWPLDGDMWEYQMNFLAERGYRVIAYDRRGFGRSSQPWSGYDYDTFADDLAELME 82 Query: 136 ELDSGPVVWVGLSMGGMVGQELALRHPA--LVRALVLANTT----------SGYPQAARD 183 LD VG SMGG RH + + +A +L T G P Sbjct: 83 HLDLQGATLVGFSMGGGDVARYIGRHGSKRVAKAALLGAVTPIFGQAPDFPQGVPAEVFA 142 Query: 184 AWQQRIATVRAEGIEAIADAVMGRYFHEDFRAAQAATVARYRHRLVATDAVGYVGCCHAV 243 ++ + RA+ I A G A +A+ + + G + C A Sbjct: 143 GIKEGLLKDRAQFISDFAAVFFGTNRPGKADAVSPGVLAQTFNIAMLASLKGTLDCVTAF 202 Query: 244 GTVDTAARLGEIKLPALVIAGELDQGTPV-SMAQVLVEGIAGARLEVIAGASH-ISAVEQ 301 D A + + P LVI G+ DQ P+ S +++ E + GA+L++ +GA H + A + Sbjct: 203 SGTDFRADIKKFNFPTLVIHGDDDQIVPLQSTGKLVAEMVPGAQLKIYSGAPHALCATHK 262 Query: 302 PGAFAELVCGFIEG 315 A+L+ F++G Sbjct: 263 DQVNADLLA-FLKG 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,061,063 Number of extensions: 46172 Number of successful extensions: 223 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 5 Length of query: 316 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 231 Effective length of database: 1,217,395 Effective search space: 281218245 Effective search space used: 281218245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)