BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.3869.p
         (170 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313274|ref|YP_003777366.1| protocatechuate 3,4-dioxygenase...   328   1e-91

>gi|300313274|ref|YP_003777366.1| protocatechuate 3,4-dioxygenase
           subunit alpha [Herbaspirillum seropedicae SmR1]
          Length = 170

 Score =  328 bits (842), Expect = 1e-91,   Method: Compositional matrix adjust.
 Identities = 159/170 (93%), Positives = 165/170 (97%)

Query: 1   MSNITTSQTIGPFPHEAWAWAVELTARVDSSAPQVKISGAILDGDGVPINDAWAEAWLPG 60
           MSNITTSQTIGPFPHEAWAWAVELT+RVDSSAPQVKI+GAILDGDGVPINDAWAEAWLPG
Sbjct: 1   MSNITTSQTIGPFPHEAWAWAVELTSRVDSSAPQVKINGAILDGDGVPINDAWAEAWLPG 60

Query: 61  SAGAETSHAIPGYRRVPTDEEGKFTFSISQPQPPAGKPVAYVTVFARGLVKHQFSAVFLE 120
           SA  E++HAIPGYRRVPT+EEG F FSISQPQ PAGKPVAYVTVFARGLVKHQF+AVFLE
Sbjct: 61  SAPVESAHAIPGYRRVPTNEEGGFAFSISQPQAPAGKPVAYVTVFARGLVKHQFTAVFLE 120

Query: 121 DDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGARETVFFDYI 170
           DDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGARETVFFDYI
Sbjct: 121 DDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGARETVFFDYI 170


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.317    0.133    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 621,339
Number of extensions: 27231
Number of successful extensions: 83
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 83
Number of HSP's successfully gapped: 1
Length of query: 170
Length of database: 1,619,870
Length adjustment: 79
Effective length of query: 91
Effective length of database: 1,245,805
Effective search space: 113368255
Effective search space used: 113368255
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 127 (53.5 bits)