BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3852.p (206 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313257|ref|YP_003777349.1| transmembrane protein [Herbaspi... 374 e-105 >gi|300313257|ref|YP_003777349.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 202 Score = 374 bits (960), Expect = e-105, Method: Compositional matrix adjust. Identities = 176/202 (87%), Positives = 190/202 (94%) Query: 1 MSGRSGERAAERIRLAVILVILVGAALGSFWVLQALRATGDQQPAQRPRGKPDYYLENFS 60 MSGRSGERAA+RIR+ VILV+L+GAALGS WVLQ++RA GDQQ AQRPRGKPDYYLENFS Sbjct: 1 MSGRSGERAADRIRIGVILVLLIGAALGSLWVLQSMRAAGDQQAAQRPRGKPDYYLENFS 60 Query: 61 YVKMGPTGLPRYDVSGVKMVHFPSDDSFEITKPVVHNLDQDQAPMQLYSDTARVTDDQTQ 120 YVKMGP GLPRYD+SGVKMVH+P+DDSFEITKPVVHNLDQ + PM+LYSDTARVTD+QTQ Sbjct: 61 YVKMGPNGLPRYDMSGVKMVHYPTDDSFEITKPVVHNLDQGKPPMELYSDTARVTDEQTQ 120 Query: 121 IHMYGNANAIRAPYKGHEQMHLVSQYLLLLPDDEIVKTDKPVTMTMGTTRLSGVGMVANN 180 IHMYGNANAIRAPYKG EQM +VS YLLLLPDDEIVKTDKPV MTMGTTRL+GVGMVANN Sbjct: 121 IHMYGNANAIRAPYKGREQMQVVSDYLLLLPDDEIVKTDKPVAMTMGTTRLNGVGMVANN 180 Query: 181 ATQVVQLFGNVRGYYEPTPKTR 202 ATQVVQLFGNVRGYYEPTPK R Sbjct: 181 ATQVVQLFGNVRGYYEPTPKKR 202 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 677,416 Number of extensions: 28002 Number of successful extensions: 72 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 72 Number of HSP's successfully gapped: 1 Length of query: 206 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 125 Effective length of database: 1,236,335 Effective search space: 154541875 Effective search space used: 154541875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)