BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3849.p (663 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 1197 0.0 gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 244 1e-65 gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 226 4e-60 gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 157 2e-39 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 1197 bits (3098), Expect = 0.0, Method: Compositional matrix adjust. Identities = 631/663 (95%), Positives = 641/663 (96%) Query: 1 MFSGLELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLAFAEDNETTHALAEF 60 MFSGLELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGL FAEDNETTHAL EF Sbjct: 1 MFSGLELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLGFAEDNETTHALGEF 60 Query: 61 GVVFLMFSIGLEFSLSKLSAMRHIVFGLGVAQVATTIAATMLLAWAAAMFLPQFLNVSWQ 120 GVVFLMFSIGLEFSL+KLSAMRHIVFGLGVAQVATTIAATM+L W AA FLPQF N+SWQ Sbjct: 61 GVVFLMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQ 120 Query: 121 AAFALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALANNS 180 AAFALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALAN S Sbjct: 121 AAFALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALANES 180 Query: 181 GSIMVTLGWAVGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWIT 240 G+IMVTLGWA GKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWIT Sbjct: 181 GNIMVTLGWAAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWIT 240 Query: 241 EKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTLYDHWF 300 EKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRT+ DHWF Sbjct: 241 EKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHWF 300 Query: 301 LALLLLTGPVLLKFALIAGLARLFGSTTGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDP 360 L LLLLTGPVLLKF LIA LARLFGST GVALRVGLGLAQAGEFGFVLLSQAGGLKLVDP Sbjct: 301 LVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDP 360 Query: 361 LLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKHVIIA 420 +LIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKHVIIA Sbjct: 361 MLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKHVIIA 420 Query: 421 GFGRSGQSLARLLEEEGIPYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHRAA 480 GFGRSGQSLARLLEEEGI YHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHRAA Sbjct: 421 GFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHRAA 480 Query: 481 ALVVTYASTPSALKVLHHAHELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSLML 540 ALVVTYASTPSALKVLHHA ELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSLML Sbjct: 481 ALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSLML 540 Query: 541 ASHALVMLGVPLRRVVHRVQDARDERYASLRGYFHGMSDAEADGVGMNVRLQSVTLIGGA 600 ASHALVMLGVPLRRVVHRVQDARDERYASLRGYFHGMSD EADGVGMNVRLQ+VTLIGGA Sbjct: 541 ASHALVMLGVPLRRVVHRVQDARDERYASLRGYFHGMSDGEADGVGMNVRLQAVTLIGGA 600 Query: 601 SAVGHTLAEIDLAGQCGAEVAMLRRGKQRVEAPEQTVLMAGDIVVLRGSSESILRAEKRL 660 SA+GHTLAEIDL CGAEVA LRR KQR+E PEQTVLMAGDIVVLRGSSES+LRAEKRL Sbjct: 601 SALGHTLAEIDLRAHCGAEVASLRRNKQRIEPPEQTVLMAGDIVVLRGSSESLLRAEKRL 660 Query: 661 LSK 663 LSK Sbjct: 661 LSK 663 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 244 bits (624), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 178/534 (33%), Positives = 278/534 (52%), Gaps = 34/534 (6%) Query: 11 LLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLAFAEDNETTHALAEFGVVFLMFSIG 70 L+ A + G +A R+ ++PP++GYLV GI IGP F D + LAE GV+ LMF +G Sbjct: 15 LVLAFIFGTLAHRL-KMPPLVGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVG 73 Query: 71 LEFSLSKLSAMRHIVFGLGVAQVATTIAATMLLAWAAAMFLPQFLNVSWQAAFALGGALT 130 L FSL L ++++I VAQ+ M L W L F G AL+ Sbjct: 74 LHFSLKDLLSVKNIAIPGAVAQITIATLLGMALGW--------LLGWPVGEGFLFGLALS 125 Query: 131 MSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALAN---------NSG 181 ++ST ++ K L ER +ET+ GR +G L+ +D+A+V L+++PAL+ + Sbjct: 126 VASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGN 185 Query: 182 SIMVTLGWAVGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWITE 241 ++++TLG +GK + A++L G +++ + S+ELF L +L I LG A + Sbjct: 186 AVLLTLGITLGKVVAFAAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSA 245 Query: 242 KA-GLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTLYDHWF 300 G+S ALGAF AGM++SE+E H+ E+ RD LFF+++GML + + Sbjct: 246 YVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVAREPL 305 Query: 301 LAL----LLLTGPVLLKFALIAGLARLFGSTTGVALRVGLGLAQAGEFGFVLLSQAGGLK 356 L L +++ G L ++ R FG G AL + LAQ GEF F+L + L Sbjct: 306 LVLATVLIIVVGKSLGALLIV----RAFGYPNGTALTISASLAQIGEFSFILATLGLSLD 361 Query: 357 LVDPLLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKH 416 L+ P+ +IL +LS+L P + + D ++ A + + + +Q H Sbjct: 362 LLSPMARDLILGGAILSILLNPLLFSLLD----RYEARQ---PKAPQGGDEPQKVELQDH 414 Query: 417 VIIAGFGRSGQSLARLLEEEGIPYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGI 476 V++ GFGR G+ + L +G P+ +E E ++ R G YG+AA+ E L AA I Sbjct: 415 VVLVGFGRVGRGIGEQLRAQGKPFVVIEAQLENLEALRAAGVPALYGNAAQSELLQAAAI 474 Query: 477 HRAAALVVTYASTPSALKVLHHAHELEPELPVIVRSHDDADLEKLLAAGATEVV 530 RA L+V A +V+ +A EL P L V+ R+H DA++E L GA V+ Sbjct: 475 DRARWLLVAIPEVFEAGQVIENALELNPGLQVVARAHSDAEIEHLEKHGAQRVI 528 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 226 bits (577), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 190/589 (32%), Positives = 291/589 (49%), Gaps = 24/589 (4%) Query: 7 LTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLAFAEDNETTHALAEFGVVFLM 66 + L L AAV V + + L +LGYL+ GI+IGP GL + + +EFGVV L+ Sbjct: 7 IALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVLLL 66 Query: 67 FSIGLEFSLSKLSAMRHIVFGLGVAQVATTIAATMLLAWAAAMFLPQFLNVSWQAAFALG 126 F IGLE +L A+R +FG G QVA A + A +V W+ Sbjct: 67 FLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAA--------LAASVPWRIGLIGA 118 Query: 127 GALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPAL--ANNSGSIM 184 +++SSTAI L ER T G +LLFQD+A +P++ VP L A + GS Sbjct: 119 LGMSLSSTAIALATLGERKLTATPAGSAAFSILLFQDIAAIPMMAAVPLLGVAASQGS-- 176 Query: 185 VTLGW-AVGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWITEKA 243 GW G+ +V+ALL+F G LMR RI+ K +E+F LL+ + + + Sbjct: 177 -GQGWLHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSV 235 Query: 244 GLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTLYDHWFLAL 303 G+S+ALG F+AG+L++++E++H +E D++ F+ +LLGLFFI +GM ++ + + L Sbjct: 236 GMSMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRIL 295 Query: 304 LLLTGPVLLKFALIAGLARLFGSTTGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDPLLI 363 ++ G + +K ++ L R FG G L L+Q GEF F++ A K++ P Sbjct: 296 GMVLGFLAIKTCVMWALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETS 355 Query: 364 QVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKH-VIIAGF 422 +++ + LSML TP +L D ++++ WM + + Q H VIIAGF Sbjct: 356 SLLVVVVALSMLVTPLLLLVHDRLLVR-----WMEGG---KRRPEDKIDPQDHAVIIAGF 407 Query: 423 GRSGQSLARLLEEEGIPYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHRAAAL 482 GR GQ + RLL GI L+ DP+ + R G V YGDA R + L AG +A L Sbjct: 408 GRFGQIIGRLLAANGIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVL 467 Query: 483 VVTYASTPSALKVLHHAHELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSLMLAS 542 V+ +AL+++ E P L + R+ + L+ G T + E E +L + Sbjct: 468 VIAIDDAQAALEMVDKVREEFPHLQIAARARNVTHYYDLMDRGVTVLERETFESALHMGR 527 Query: 543 HALVMLGVPLRRVVHRVQDARDERYASLRG-YFHGMSDAEADGVGMNVR 590 L L R R +L Y H + A+ + + R Sbjct: 528 QVLQQLDFGAYRAREAAMKFRAHNLKTLEALYPHYKNQAQMVSMAVQAR 576 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 157 bits (398), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 117/391 (29%), Positives = 209/391 (53%), Gaps = 19/391 (4%) Query: 6 ELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLAF--AEDNETTHALAEFGVV 63 ++ + +L A ++ VV F ++ P +LGY+V G+IIGPH + +D +T H L+E GV+ Sbjct: 10 DMAIIMLIAGIVTVV-FNKLKQPVVLGYIVAGVIIGPHTPPYDLIQDEKTVHILSELGVI 68 Query: 64 FLMFSIGLEFSLSKLSAMRHIVFGLGVAQVATTIAATMLLAWAAAMFLPQFLNVSWQ--A 121 FL+FS+GLEFSL KL+ + F +G A A +L+ W F W+ Sbjct: 69 FLLFSLGLEFSLKKLAKVGATAF-IGAA------AEILLMIWIGYEIGLYF---GWKRMD 118 Query: 122 AFALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALANNSG 181 A LG L +SST I+ K L E + + I G+L+ +D+ + ++ ++ +A SG Sbjct: 119 AIFLGAMLAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGVAT-SG 177 Query: 182 SIMVT-LGWAVGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWIT 240 S+ T + VGK ++ + + L G + + + +S E+ ++ +L + G + Sbjct: 178 SVDSTEVFTTVGKLVLFMTVSLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCLLV 237 Query: 241 EKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTLYDHWF 300 K S+ALGAF+ G +++E+ H++E ++ RD+ +FF+ IG+L + L +W Sbjct: 238 MKLQYSVALGAFLVGAVMAESRHLHRIERLVEPIRDMFSAIFFVAIGLLFDPNVLVKYW- 296 Query: 301 LALLLLTGPVLLKFALIAGLARLFGSTTG-VALRVGLGLAQAGEFGFVLLSQAGGLKLVD 359 + + ++T V+ + GL G +RVG+GLAQ GEF F++ + LK+ Sbjct: 297 MPITVITLAVVFGKLISCGLGTFIAGQGGRTPMRVGMGLAQIGEFSFIIAALGQSLKVTS 356 Query: 360 PLLIQVILAAMVLSMLATPFILAKSDAIVMK 390 L +++A ++ L TP+++ +D + K Sbjct: 357 DFLYPIVVAVSAVTALLTPYLIKAADPLSAK 387 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,718,694 Number of extensions: 67994 Number of successful extensions: 428 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 418 Number of HSP's successfully gapped: 5 Length of query: 663 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 572 Effective length of database: 1,188,985 Effective search space: 680099420 Effective search space used: 680099420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)