BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3840.p (495 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310625|ref|YP_003774717.1| hemolysin activation translocat... 638 0.0 gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ... 369 e-103 gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ... 59 6e-10 >gi|300310625|ref|YP_003774717.1| hemolysin activation translocator protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 638 bits (1645), Expect = 0.0, Method: Compositional matrix adjust. Identities = 302/494 (61%), Positives = 378/494 (76%) Query: 2 LHVPEQLPALAQAAGASALPQDPFRFAQQYLDQYAGQCVGKDGLNLIVKRLTALILSKGY 61 L PEQ+ + G AL DPF F Q YL YAG C+G+ G+ I++R+T IL +GY Sbjct: 88 LAFPEQVSEAVRRTGGFALQMDPFHFIQTYLAGYAGSCLGQQGIETILRRVTGQILERGY 147 Query: 62 STTRLGIAAQDLSSHRLELTLVPGMIRAIKFSSDAAGTTWKSAFPTGPGQLLNLRDLEQG 121 STTR+GI QDLS+ +L +TLVPG+I AI+F A +++SAFPT G LLNLRDLEQG Sbjct: 148 STTRVGIGQQDLSTGQLSITLVPGIIHAIRFKDPAITASYRSAFPTQAGALLNLRDLEQG 207 Query: 122 LEQMKRVPTQDVTMEIVPGELPGESDVVITVKRKTPWKLTAALDDSGAKGTGKLQSSVNL 181 LEQMKRV +QDV M+I+P ++PG+SDVV+ VKR PWKLT +LDDSGAK TGK Q+ +NL Sbjct: 208 LEQMKRVASQDVDMQILPTDVPGQSDVVLEVKRIKPWKLTLSLDDSGAKSTGKYQAGINL 267 Query: 182 AWDNLFGLNDLFNIGLNTDADRRGQQRGTHGSNIYYSIPYGYWTFSAAASTYAYHQQIAG 241 A D+L G+NDLFN G ++DADR GQ RGT G+N YS P GYWTF + ST YHQ+IAG Sbjct: 268 AIDDLLGINDLFNAGFSSDADRMGQARGTTGNNFSYSAPAGYWTFGVSGSTSRYHQRIAG 327 Query: 242 LFQTFVSSGKSQNLEFTVQRLFYRDQVEKHSVQFKVAKRWNRSYVDDTELEVQRRNTTAA 301 LFQTFVSSG S+NLE VQ+LF RD +K+S+Q ++ KRW+RSY+DDTE+EVQRRNTT A Sbjct: 328 LFQTFVSSGHSENLEVRVQKLFQRDAQQKNSLQLRIGKRWSRSYIDDTEIEVQRRNTTFA 387 Query: 302 ELAWLYTRYIGDAQFNLVLANRWGVSWFNGQPDLEPRLADAPTYRYSLQTIDASLYAPFK 361 EL W++ Y GD Q + LANRWGVSWFNG D RL+D PTYRYS+QTIDA++ APFK Sbjct: 388 ELGWMHRHYFGDVQLDATLANRWGVSWFNGAADAPDRLSDGPTYRYSMQTIDATVVAPFK 447 Query: 362 LGTQSFNYIGALRGQSTHSTLFLSDQFAIGNRYTVRGFDGEQTLSAERGVYLRNEIEMPL 421 LG QS Y+G LRGQ + S L+L+DQFAIG+RYTVRGFDGE TL++E GV+LRN++E+ L Sbjct: 448 LGGQSLTYVGTLRGQHSRSRLYLTDQFAIGSRYTVRGFDGELTLASESGVFLRNDLEIAL 507 Query: 422 GETRQALYAGLDAGRVFGPGAQLLVGTKMAGAAIGLRGNAAGVRYDVFAGWALVKPAGLK 481 G T ALYAGLDAGR++GP L G +AGAAIGLRG +G+ Y++FAGWALV+P + Sbjct: 508 GSTGAALYAGLDAGRLYGPSVANLQGDTLAGAAIGLRGAVSGLHYELFAGWALVRPEQFQ 567 Query: 482 TATPSAGFSISYQY 495 TA P+AGF++ YQ+ Sbjct: 568 TAMPAAGFNLIYQF 581 >gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 584 Score = 369 bits (946), Expect = e-103, Method: Compositional matrix adjust. Identities = 200/470 (42%), Positives = 284/470 (60%), Gaps = 13/470 (2%) Query: 37 GQCVGKDGLNLIVKRLTALILSKGYSTTRLGIAAQDLSSHRLELTLVPGMIRAIKFS--S 94 G+C+G G+N ++ L I+++GY TTR+ QD++S L LT+VPG IRAI+F+ + Sbjct: 117 GRCLGVQGINAVMSALQNAIVARGYVTTRILAQPQDIASGELRLTIVPGKIRAIRFADNA 176 Query: 95 DAAGTTWKSAFPTGPGQLLNLRDLEQGLEQMKRVPTQDVTMEIVPGE----LPGESDVVI 150 D T W +A P PG LLNLRD+EQGLE +KRVPT + ++I P E PG+SD+VI Sbjct: 177 DPRATQW-NALPASPGDLLNLRDIEQGLENLKRVPTAEADVQIAPSEGKDAAPGDSDLVI 235 Query: 151 TVKRKTPWKLTAALDDSGAKGTGKLQSSVNLAWDNLFGLNDLFNIGLNTD-ADRRGQQRG 209 + ++ P +LT ++DDSG K TGK Q S ++ DN LNDLF I + D RG Sbjct: 236 SYQQGIPIRLTLSVDDSGTKATGKYQGSATVSLDNWLTLNDLFYISFSHDLGGGDPGARG 295 Query: 210 THGSNIYYSIPYGYWTFSAAASTYAYHQQIAGLFQTFVSSGKSQNLEFTVQRLFYRDQVE 269 + ++YS+P GYW + S+ YHQ +AG+ QT++ SG+S+ E + RL YRD V Sbjct: 296 SQSQVVHYSLPLGYWALAFTNSSNRYHQAVAGINQTYLYSGQSETNELKLSRLVYRDAVR 355 Query: 270 KHSVQFKVAKRWNRSYVDDTELEVQRRNTTAAELAWLYTRYIGDAQFNLVLANRWGVSWF 329 K ++ FK R + +YVDDTE++VQRR E+ + +IG+ +L LA + G F Sbjct: 356 KTTLSFKGFVRSSSNYVDDTEVQVQRRRVAGWEMGLAHREFIGEGTLDLALAYKRGTGAF 415 Query: 330 NGQPDLEPRLADAPTYRYSLQTIDASLYAPFKL----GTQSFNYIGALRGQSTHSTLFLS 385 + E + T R+ L T D +L APF L G Q+ Y R Q + L Sbjct: 416 DAMAAPEEAFGEG-TSRFRLMTADLNLNAPFALPMPWGKQAMRYTLGARAQWNRTPLTPQ 474 Query: 386 DQFAIGNRYTVRGFDGEQTLSAERGVYLRNEIEMPLGETRQALYAGLDAGRVFGPGAQLL 445 D+F++G RY+VRGFDGE L AERG Y+RNE+ LG ++Q +YAGLD G V GP +LL Sbjct: 475 DKFSLGGRYSVRGFDGESNLLAERGWYVRNELAAALGASKQEIYAGLDYGEVGGPSTELL 534 Query: 446 VGTKMAGAAIGLRGNAAGVRYDVFAGWALVKPAGLKTATPSAGFSISYQY 495 VG ++AGA +GLRG Y+VFAG + +P KT+ +AG S+ + + Sbjct: 535 VGRRLAGAVLGLRGALGRTNYEVFAGVPISQPDRFKTSPLTAGMSLMWSF 584 >gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 569 Score = 59.3 bits (142), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 109/477 (22%), Positives = 192/477 (40%), Gaps = 89/477 (18%) Query: 15 AGASALPQDPFRFAQQYLDQYAGQCVGKDGLNLIVKRLTALILSKGYSTTRLGIAAQDLS 74 AG + LPQD + A + ++GQ + ++ L L S GY+ ++ + Q+LS Sbjct: 61 AGNTLLPQDQVQAA---VAPFSGQGRVYGDIQQALEALENLYRSAGYTAVQVHVPEQELS 117 Query: 75 SHRLELTLVPGMIRAIKFSSDA--AGTTWKSAFPT-GPGQLLNLRDLEQGLEQMKRVPTQ 131 S + + +V ++ ++ S + + +++ P G NLR + + ++ P + Sbjct: 118 SGEVRIDVVETVVGKVQVSENRHFSQANIRASIPALKEGVPPNLRRISESVQLANDNPAK 177 Query: 132 DVTMEIVPGELPGESDVVITVKRKTPWKLTAALDDSGAKGTGKLQSSVNLAWDNLFGLND 191 V + + + + D I VK P ++ LD++G+ TG+ ++ V + NLF + Sbjct: 178 QVNVTLSAAQSGDQIDAKIEVKDDDPLRVMLNLDNTGSADTGRWRTGVAIQHSNLFNRDH 237 Query: 192 LFNIGLNTDADRRGQQRGTHGSNI-YYSIPYGYWTFSAAAS-TYAYHQQIAGLFQTFVSS 249 + + T D GS + YS+ Y + S + Y ++ VSS Sbjct: 238 VATVAYTTSPD------SPSGSKVDLYSLGYRLPLYELGDSLDFIYG-------KSSVSS 284 Query: 250 GKSQNLEFTVQRLFYRDQVEKHSVQFKVAKRWNRSYVDDTE--------LEVQRRNTTAA 301 G++ L T+ + R +V A RWN + + E + ++ +++ A Sbjct: 285 GQTLALNSTLN-ITGRGEV--------YALRWNHFFARNGEWSSKLVLGADYKKVDSSCA 335 Query: 302 ELAWLYTRYIGDAQFNLVL------------ANRWGV-SWFNGQPDLEPRLADAPTYRY- 347 L + A N L A R GV + + +A R Sbjct: 336 INGGL----LNGATLNTCLPYSTLPLSLTYSAQRQGVGEVIDANIGVARNMATGQARRND 391 Query: 348 SLQTIDASLYAPFKLGTQSFNYIGALRGQSTHSTLFLSD--------------------Q 387 +LQ D Y+ F G QS + A+RG ++ F +D Q Sbjct: 392 ALQVTDR--YSLFTNGRQSMDNFIAVRGGASWLKAFQNDWQMRLAGNLQLSPHALPAVEQ 449 Query: 388 FAIGNRYTVRGFDGEQTLSAERGVYLRNEIEMP--------LGETRQALYAGLDAGR 436 F + VRGF E+ +SA+ GV L +E+ P G R L A +DAGR Sbjct: 450 FGLAGATAVRGFT-ERAVSADSGVVLNSELYTPDLLAKSELKGSMR--LLAFVDAGR 503 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,530,584 Number of extensions: 67045 Number of successful extensions: 193 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 3 Length of query: 495 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 406 Effective length of database: 1,198,455 Effective search space: 486572730 Effective search space used: 486572730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)