BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3831.p (540 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313221|ref|YP_003777313.1| periplasmic glucans biosynthesi... 898 0.0 gi|300312745|ref|YP_003776837.1| periplasmic glucans biosynthesi... 346 3e-96 gi|300314061|ref|YP_003778153.1| periplasmic glucan biosynthesis... 307 1e-84 >gi|300313221|ref|YP_003777313.1| periplasmic glucans biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 898 bits (2320), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/543 (83%), Positives = 479/543 (88%), Gaps = 3/543 (0%) Query: 1 MTSRRHFLTAAGAASALAALGVSPQ---ALAAQGMQMGRAQPFSFEQLIARARDMARTPY 57 MTSRR FL A GA + AAL ALAAQG++ QPFSF QLI RAR MA TPY Sbjct: 2 MTSRRRFLCATGATATAAALSALGLPSGALAAQGIETSAPQPFSFAQLIERARQMASTPY 61 Query: 58 QAPPAVPAAILDQIDYDAHGKIHFKTDLAVFAQGPGAFPLTFFHLGKFFRVPVRMFILDG 117 Q A PAAILD+IDYDAHGKI FK D A FA GPG FPLTFFHLGKFFRVPVRMF+L+G Sbjct: 62 QPAAAAPAAILDRIDYDAHGKIRFKPDRAAFATGPGPFPLTFFHLGKFFRVPVRMFLLEG 121 Query: 118 QGDATVPVAARELSYRDDYFDMPADSPAHRLPPGSGFAGFRFQESRLADQSRKDWRKNDW 177 GDAT P AREL YRDDYFDMPADSPAH LPPGSGFAGFRFQESRL DQ KDWRKNDW Sbjct: 122 AGDATAPTQARELRYRDDYFDMPADSPAHALPPGSGFAGFRFQESRLGDQQVKDWRKNDW 181 Query: 178 VAFLGASYFRAIGDLYQYGLSARGIAIDVAQADRPEEFPDFTQFFFEPQAPGSETVVVYA 237 VAFLGASYFRAIGDLYQYGLSARG+AIDVA A + EEFPDFTQFFFEP APGS+TVVVYA Sbjct: 182 VAFLGASYFRAIGDLYQYGLSARGVAIDVAVAGKSEEFPDFTQFFFEPGAPGSDTVVVYA 241 Query: 238 LLDGPSITGAYRFALQRGAGVGMEIDKALFLRRDVARLGLAPTTSMYWFSETVKGTGVDW 297 LLDGPSITGAYRFA+QRG GV MEIDK LFLRRDV RLGLAP TSMYW+SETVKGTGVDW Sbjct: 242 LLDGPSITGAYRFAMQRGQGVTMEIDKQLFLRRDVERLGLAPATSMYWYSETVKGTGVDW 301 Query: 298 RPEVHDSDGLAIWSGAGEHLWRPLNNPPRTMASAFGDQHLRGFGLLQRDRDFDHYLDGVH 357 RPEVHDSDGLAIW+GAGEHLWRPLNNPPRT+AS+F D+ RGFGL+QRDRDFDHYLDGVH Sbjct: 302 RPEVHDSDGLAIWNGAGEHLWRPLNNPPRTIASSFADRQPRGFGLMQRDRDFDHYLDGVH 361 Query: 358 YERRPSLWVEPLGDWGEGAVQLIELRTDDEIHDNIVAMWVPKAPARAGNSYRLRYRLYWQ 417 YERRPSLWVEPLGDWGEG VQLIELRTDDEIHDNIVAMWVPKA ARAG+ Y+LRYRL+WQ Sbjct: 362 YERRPSLWVEPLGDWGEGQVQLIELRTDDEIHDNIVAMWVPKAAARAGDRYQLRYRLHWQ 421 Query: 418 KDEPFPTPLARCVATRLGRGGQPGQARPQQVRKFMVEFKGAPLENLPFGVKPEAVITASR 477 + PFP+ LARCVATRLGRGGQPGQ RP QVRKFMVEFKG PL+ LPFGVKPEAVI+ASR Sbjct: 422 SETPFPSALARCVATRLGRGGQPGQPRPPQVRKFMVEFKGPPLQALPFGVKPEAVISASR 481 Query: 478 GSISYVYTEALPNGVAGHWRAQFDLTADGQEPVELRLFLRHQGQALSETWLFQYHPFVAR 537 GSISYVYTEALPNGV GHWRAQFDLTA+G+EPVELRLFLRHQGQALSETWLFQYHPF+AR Sbjct: 482 GSISYVYTEALPNGVPGHWRAQFDLTAEGEEPVELRLFLRHQGQALSETWLFQYHPFIAR 541 Query: 538 EVY 540 EVY Sbjct: 542 EVY 544 >gi|300312745|ref|YP_003776837.1| periplasmic glucans biosynthesis G protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 346 bits (887), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 209/501 (41%), Positives = 281/501 (56%), Gaps = 22/501 (4%) Query: 40 FSFEQLIARARDMARTPYQAPPAVPAAILDQIDYDAHGKIHFKTDLAVFAQGPGAFPLTF 99 F F + ARA+ +A Y+ P L + Y+ +I+F+ D + + F L+F Sbjct: 55 FDFNTVAARAKALAGKSYKKPADELPKSLKDLSYEQASQIYFRDDKSYWRAQKLPFELSF 114 Query: 100 FHLGKFFRVPVRMFILDGQGDATVPVAARELSYRDDYFDMPAD-SPAHRLPPGSGFAGFR 158 HLG + P+++ + V A RE+ + FD A S R GFAGFR Sbjct: 115 IHLGASYTEPIKV-------NEVVGDAVREIHFNPALFDYSATRSVDPRSLRNLGFAGFR 167 Query: 159 FQESRLADQSRKDWRKNDWVAFLGASYFRAIGDLYQYGLSARGIAIDVAQADRPEEFPDF 218 R S K K+D + F GASYFRA+G YGLSARG+AID A EEFP F Sbjct: 168 V---RFPVNSAK--VKDDVLTFHGASYFRALGKGQSYGLSARGLAIDTALYSG-EEFPRF 221 Query: 219 TQFFFEPQAPGSETVVVYALLDGPSITGAYRFALQRGAGVGMEIDKALFLRRDVARLGLA 278 T+F+ E + S+ +V+YALLD P TGAYRF L+ GA M++ L+LR +V +LG+A Sbjct: 222 TEFWLERPSAESKELVIYALLDSPRATGAYRFVLKPGADTVMDVKAQLYLRSNVTKLGIA 281 Query: 279 PTTSMYWFSETVKGTGVDWRPEVHDSDGLAIWSGAGEHLWRPLNNPPRTMASAFGDQHLR 338 P TSM+ F E G D+RPEVHDSDGLA+ SG GE LWRPL NP R + ++F + Sbjct: 282 PLTSMFLFGENQPGPADDFRPEVHDSDGLAMQSGTGEWLWRPLVNPKRLLVTSFSFDNPL 341 Query: 339 GFGLLQRDRDFDHYLD-GVHYERRPSLWVEPLGDWGEGAVQLIELRTDDEIHDNIVAMWV 397 GFGL+QRDR F HY D +Y+ RPS WVEP G WG G V+L+++ T DE +DNIVA W+ Sbjct: 342 GFGLMQRDRSFSHYEDLDYNYQARPSAWVEPKGKWGSGRVELVQIPTPDETNDNIVAYWI 401 Query: 398 PKAPARAGNSYRLRYRLYWQKDEPFPTPLARCVATRLGRGGQPGQ-ARPQQVRKFMVEFK 456 P + G + + YRL WQKD PLA T G G +P Q + V++ ++F Sbjct: 402 PNTLPKPGQPFNVEYRLSWQKDNEKRPPLAYVTQTLFGHGYRPKQEGKTDDVQQLSIDFD 461 Query: 457 GAPLENLPFGVKPEAVITA-SRGSISYVYTEALPNGVAGHWRAQFDL-TADGQEPVELRL 514 G L+ LP + E V+ A + G ++ V T N G WR + L + +PVELR Sbjct: 462 GGNLKKLPANARVEGVVEADANGKLASVTTRK--NEATGGWRVEVKLRRTEENKPVELRG 519 Query: 515 FLRHQ--GQALSETWLFQYHP 533 FLR+ G LSETW + P Sbjct: 520 FLRNANGGTTLSETWSYILPP 540 >gi|300314061|ref|YP_003778153.1| periplasmic glucan biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 517 Score = 307 bits (787), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 199/524 (37%), Positives = 279/524 (53%), Gaps = 39/524 (7%) Query: 26 ALAAQGMQMGRAQPFSFEQLIARARDMARTPYQAPPAVPAAILDQIDYDAHGKIHFKTDL 85 L A Q +Q F Q +ARA+D+A PYQ P + + + + + KI K++ Sbjct: 9 CLLAASAQAQTSQGFDLNQAVARAKDLAAKPYQKPVSNLSPTFAAMQFADYMKIQPKSEK 68 Query: 86 AVFAQGPGAFPLTFFHLGKFFRVPVRMF-ILDGQGDATVPVAARELSYRDDYFD-----M 139 + F L F+H G F PV + I+DG+ +++ Y D F+ + Sbjct: 69 FEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDGR--------VQDVGYSTDRFNFGDLNL 120 Query: 140 PADSPAHRLPPGSGFAGFRFQESRLADQSRKDWRKNDWVAFLGASYFRAIGDLYQYGLSA 199 DS AH G+AGFR + +D ++++ ++ LGASYFR IG YGLSA Sbjct: 121 AKDSTAHL-----GYAGFR-----VVYPINRDDKEDEVMSVLGASYFRVIGKGQVYGLSA 170 Query: 200 RGIAIDVAQADRPEEFPDFTQFFFEPQAPGSETVVVYALLDGPSITGAYRFALQRGAGVG 259 RG+AID A EEFP FT+F+ + APG + + YALLD P TGAY+F L G Sbjct: 171 RGLAIDTAMMSG-EEFPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKDAL 229 Query: 260 MEIDKALFLRRDVARLGLAPTTSMYWFSETVKGTGVDWRPEVHDSDGLAIWSGAGEHLWR 319 +++ +FLR +V RLG+AP TSM+ F + + ++RP +HDS+GLAI +G GE LWR Sbjct: 230 LDVQARVFLRGEVGRLGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWLWR 289 Query: 320 PLNNPPRTMASAFGDQHLRGFGLLQRDRDFDHYLD-GVHYERRPSLWVEPLGDWGEGAVQ 378 PLN+P S+F + RGFGLLQR R+F Y D Y+ RPS W+EP GDWG+GAVQ Sbjct: 290 PLNDPQNVEVSSFEVSNPRGFGLLQRGREFSAYEDLKDRYDLRPSAWIEPKGDWGKGAVQ 349 Query: 379 LIELRTDDEIHDNIVAMWVP-KAPARAGNSYRLRYRLYWQKDEP-FPTPLARCVATRLGR 436 L+E+ T DE +DNIVA W+P + PAR G YR++W DEP V Sbjct: 350 LVEIPTADETNDNIVAYWLPAQLPAR-GTPLAYDYRIHWTMDEPAILNNEVGWVKQTFHT 408 Query: 437 GGQPGQARPQQVRKF------MVEFKGAPLENLPFGVKPEAVITASRGSISYVYTEALPN 490 G+ QA +R F +V+F G PL LP G + ++ S + + PN Sbjct: 409 DGELTQA--NLIRAFDGTTALLVDFTGGPLGALPAGTPVQPQVSVSNNG-ELIDVQLQPN 465 Query: 491 GVAGHWRAQFDLTA-DGQEPVELRLFLRHQGQALSETWLFQYHP 533 WR + + + VELR L G+ L+ETW +Q P Sbjct: 466 PAINGWRLTLRVKIRNVAQAVELRAALSANGKPLTETWSYQLPP 509 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,901,777 Number of extensions: 89475 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 3 Length of query: 540 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 450 Effective length of database: 1,193,720 Effective search space: 537174000 Effective search space used: 537174000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)