BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3822.p (1558 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313212|ref|YP_003777304.1| glutamate synthase large subuni... 3182 0.0 gi|300312905|ref|YP_003776997.1| glutamate synthase [Herbaspiril... 132 3e-31 >gi|300313212|ref|YP_003777304.1| glutamate synthase large subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 1558 Score = 3182 bits (8251), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1542/1558 (98%), Positives = 1551/1558 (99%) Query: 1 MHAQGLYDPANEHDACGVGFIAHIKGKKSHSIVDQGLLILKNLDHRGAVGADALMGDGAG 60 MHAQGLYDPANEHDACGVGFIAHIKGKKSHSIVDQGLLILKNLDHRGAVGAD LMGDGAG Sbjct: 1 MHAQGLYDPANEHDACGVGFIAHIKGKKSHSIVDQGLLILKNLDHRGAVGADPLMGDGAG 60 Query: 61 ILIQIPDQYYREEMAKQGVDLPPPGEYGVGMIFLPKENASRIACEQEIERSVLAEGQIVL 120 ILIQIPDQYYREEM KQGVDLPPPGEYGVGM+FLPKENASRIACEQEIER+VLAEGQIVL Sbjct: 61 ILIQIPDQYYREEMTKQGVDLPPPGEYGVGMVFLPKENASRIACEQEIERAVLAEGQIVL 120 Query: 121 GWRDVPVDRDMPMSPTVREKEPVIRQIFIGRGPDVMVTDALERKLYVIRKSSGHAIQALN 180 GWRDVPVDRDMPMSPTVREKEPVIRQIFIGRGPDVMVTDALERKLYVIRKSSGHAIQALN Sbjct: 121 GWRDVPVDRDMPMSPTVREKEPVIRQIFIGRGPDVMVTDALERKLYVIRKSSGHAIQALN 180 Query: 181 LLHGKEFFVPSMSARTVVYKGLLLADQVGVYYKDLQDERCVSALALVHQRFSTNTFPEWP 240 LLHGKEFFVPSMSARTVVYKGLLLADQVGVYYKDLQDERCVSALALVHQRFSTNTFPEWP Sbjct: 181 LLHGKEFFVPSMSARTVVYKGLLLADQVGVYYKDLQDERCVSALALVHQRFSTNTFPEWP 240 Query: 241 LAHPYRLIAHNGEINTVKGNFNWMRAREGVMQSAVLGADLKKLFPLIYEGQSDTASFDNA 300 LAHPYRLIAHNGEINTVKGNFNWMRAREGVMQSAVLGADLKKLFPLIYEGQSDTASFDNA Sbjct: 241 LAHPYRLIAHNGEINTVKGNFNWMRAREGVMQSAVLGADLKKLFPLIYEGQSDTASFDNA 300 Query: 301 LELLVMAGYPLPQAMMMMVPEAWENHTSMDDNRRAFYEYHAAMMEPWDGPAALAFTDGRH 360 LELLVMAGYPLPQAMMMMVPEAWENHTSMDDNRRAFYEYHAAMMEPWDGPAALAFTDGRH Sbjct: 301 LELLVMAGYPLPQAMMMMVPEAWENHTSMDDNRRAFYEYHAAMMEPWDGPAALAFTDGRH 360 Query: 361 IGGTLDRNGLRPARYIVTDDDLVVMASESGVLPIPESKIIQKWRLQPGKMFLIDLDAGRI 420 IGGTLDRNGLRPARYIVTDDDLVVMASESGVLPIPESKIIQKWRLQPGKMFLIDLDAGRI Sbjct: 361 IGGTLDRNGLRPARYIVTDDDLVVMASESGVLPIPESKIIQKWRLQPGKMFLIDLDAGRI 420 Query: 421 IDDKELKDTYANAKPYKQWINSVRVKLDELKEEPRQPSSPLKLLDLQQVFGYTQEDVKFL 480 IDDKELKDTYANAKPYKQWINSVRVKLDELKEEPRQPSS LKLLDLQQVFGYTQEDVKFL Sbjct: 421 IDDKELKDTYANAKPYKQWINSVRVKLDELKEEPRQPSSSLKLLDLQQVFGYTQEDVKFL 480 Query: 481 MAPMASGGEEAIGSMGNDSPLAVMSNKNKPLYNYFRQLFAQVTNPPIDPIREALVMSLVS 540 MAPMASGGEEAIGSMGNDSPLAVMSNKNKPLYNYFRQLFAQVTNPPIDPIREALVMSLVS Sbjct: 481 MAPMASGGEEAIGSMGNDSPLAVMSNKNKPLYNYFRQLFAQVTNPPIDPIREALVMSLVS 540 Query: 541 FIGPKPNLLDTNNINPPMRLEVSQPVLDYDDIAKLRNISAHSGGKFKSYELNICYPVAWG 600 FIGPKPNLLDTNNINPPMRLEVSQPVLDYDDIAKLRNISAHSGGKFKSYELNICYPVAWG Sbjct: 541 FIGPKPNLLDTNNINPPMRLEVSQPVLDYDDIAKLRNISAHSGGKFKSYELNICYPVAWG 600 Query: 601 KEGIEARLASLCAKAVDAVKSGHNILIISDRKVDADQLPIPALLATSAIHQHLVSKGLRT 660 KEGIEARLASLCAKAVDAVKSGHNILIISDRKVDA+QLPIPALLATSAIHQHLVSKGLRT Sbjct: 601 KEGIEARLASLCAKAVDAVKSGHNILIISDRKVDAEQLPIPALLATSAIHQHLVSKGLRT 660 Query: 661 STGLVVETGSARETHHFALLAGYGAEAIHPYLAMDTLADLAKGLPGDLSPEKAIYNFQKA 720 STGLVVETGSARETHHFALLAGYGAEAIHPYLAMDTLAD+AKGLPGDLSPEKAIYNFQKA Sbjct: 661 STGLVVETGSARETHHFALLAGYGAEAIHPYLAMDTLADMAKGLPGDLSPEKAIYNFQKA 720 Query: 721 VGKGLLKVMSKMGISTYMSYCGAQIFEAIGLSKALVDKYFKGTASNVEGIGVFEVAEEAL 780 VGKGLLKVMSKMGISTYMSYCGAQIFEAIGLSKALVDKYFKGTASNVEGIGVFEVAEEAL Sbjct: 721 VGKGLLKVMSKMGISTYMSYCGAQIFEAIGLSKALVDKYFKGTASNVEGIGVFEVAEEAL 780 Query: 781 RLHTAAFSADPVLANALDAGGEYAFRIRGEEHMWTPDAIAKLQHSTRSNSYNTYKEYAQI 840 RLHTAAFSADPVLANALDAGGEYAFRIRGEEHMWTPDAIAKLQHSTRSNSYNTYKEYAQI Sbjct: 781 RLHTAAFSADPVLANALDAGGEYAFRIRGEEHMWTPDAIAKLQHSTRSNSYNTYKEYAQI 840 Query: 841 INDQSKRHMTLRGLFEFKIDPSKAISIDEVEPAKEIVKRFATGAMSLGSISTEAHATLAI 900 INDQSKRHMTLRGLFEFKIDPSKAISIDEVEPAKEIVKRFATGAMSLGSISTEAHATLAI Sbjct: 841 INDQSKRHMTLRGLFEFKIDPSKAISIDEVEPAKEIVKRFATGAMSLGSISTEAHATLAI 900 Query: 901 AMNRIGGKSNTGEGGEDVNRYRNELKGIPIKQGATLASEIGREHIEVDIPLLEGDSLRSR 960 AMNRIGGKSNTGEGGEDVNRYRNELKGIPIKQGATLASEIG+EHIEVDIPLLEGDSLRSR Sbjct: 901 AMNRIGGKSNTGEGGEDVNRYRNELKGIPIKQGATLASEIGKEHIEVDIPLLEGDSLRSR 960 Query: 961 IKQVASGRFGVSAEYLISADQIQIKMAQGAKPGEGGQLPGHKVSEYIAKLRVSVPGVGLI 1020 IKQVASGRFGVSAEYLISADQIQIKMAQGAKPGEGGQLPGHKVSEYIAKLRVSVPGVGLI Sbjct: 961 IKQVASGRFGVSAEYLISADQIQIKMAQGAKPGEGGQLPGHKVSEYIAKLRVSVPGVGLI 1020 Query: 1021 SPPPHHDIYSIEDLAQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAKSDHVVIAGH 1080 SPPPHHDIYSIEDLAQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAKSDHVVIAGH Sbjct: 1021 SPPPHHDIYSIEDLAQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAKSDHVVIAGH 1080 Query: 1081 DGGTGASPLSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGAMLG 1140 DGGTGASPLSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGAMLG Sbjct: 1081 DGGTGASPLSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGAMLG 1140 Query: 1141 ADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRAKFSGKPEHVVNFFFFIAEEA 1200 ADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRAKFSGKPEHVVNFFFFIAEEA Sbjct: 1141 ADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRAKFSGKPEHVVNFFFFIAEEA 1200 Query: 1201 RQIMAQLGIRKFDDLIGRADLLDRSKAIAHWKAKGLDFSRIFHQPESKLPVYHTDVQDHG 1260 RQIMAQLGIRKFD+LIGRADLLDRSKAIAHWKAKGLDFSRIFHQPESKLPVYHTD QDHG Sbjct: 1201 RQIMAQLGIRKFDELIGRADLLDRSKAIAHWKAKGLDFSRIFHQPESKLPVYHTDHQDHG 1260 Query: 1261 LDKALDHKLIAQAKIALEKGEKVSFISPIKNLNRTVGAMLSGEVAKRYGDEGLPDDTIHI 1320 LDKALDHKLIAQAK ALEKGEKVSFISPIKNLNRTVGAMLSGEVAKRYGDEGLPDDTIHI Sbjct: 1261 LDKALDHKLIAQAKTALEKGEKVSFISPIKNLNRTVGAMLSGEVAKRYGDEGLPDDTIHI 1320 Query: 1321 QLQGTAGQSAGAFLAKGVTLDLVGEGNDYVGKGLSGGRIIVRPNTEFRGRAVDNMISGNT 1380 QLQGTAGQSAGAFLAKGVTLDLVGEGNDYVGKGLSGGRIIVRPNTEFRGRAVDNMISGNT Sbjct: 1321 QLQGTAGQSAGAFLAKGVTLDLVGEGNDYVGKGLSGGRIIVRPNTEFRGRAVDNMISGNT 1380 Query: 1381 VLYGAISGEAFINGVAGERFAVRNSGAIAVVEGTGDHGCEYMTGGTVVVLGNTGRNFAAG 1440 VLYGAISGEAFINGVAGERFAVRNSGA AVVEGTGDHGCEYMTGGTVVVLGNTGRNFAAG Sbjct: 1381 VLYGAISGEAFINGVAGERFAVRNSGATAVVEGTGDHGCEYMTGGTVVVLGNTGRNFAAG 1440 Query: 1441 MSGGIAYVYDPEGDFAGKCNTAMVSLEKVLSDTEQEQTLGRNIWHSLLRGGERQTDEAIL 1500 MSGGIAYVYDPEGDFAGKCNT+MVSLEKVLSDTEQEQTLGR+IWHSL RGGERQTDEAIL Sbjct: 1441 MSGGIAYVYDPEGDFAGKCNTSMVSLEKVLSDTEQEQTLGRDIWHSLQRGGERQTDEAIL 1500 Query: 1501 RGLIERHFKYTGSTRARYLLDNWAASRAKFVKVFPTEYKRALAELAAAAETKKEKVAA 1558 RGLIERHFKYTGSTRARYLLDNWAASRAKFVKVFPTEYKRALAELAAAA+TKKEKVAA Sbjct: 1501 RGLIERHFKYTGSTRARYLLDNWAASRAKFVKVFPTEYKRALAELAAAAQTKKEKVAA 1558 >gi|300312905|ref|YP_003776997.1| glutamate synthase [Herbaspirillum seropedicae SmR1] Length = 531 Score = 132 bits (331), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 103/330 (31%), Positives = 144/330 (43%), Gaps = 56/330 (16%) Query: 865 ISIDEVEPAKEIVKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGGEDVNRYRNE 924 + D +P V F AMS GS+S A L + +TGEG V+ Y E Sbjct: 142 VGADRAQPYSMSV--FNVSAMSFGSLSANAIRALNQGAKKGNFAHDTGEG--SVSEYHRE 197 Query: 925 LKGIPIKQGATLASEIGREHIEVDIPLLEGDSLRSRIKQVASGRFGV-------SAEYLI 977 G + Q+ASG FG + E Sbjct: 198 FGG-------------------------------DLVWQIASGYFGCRNADGSFNDEKFT 226 Query: 978 SADQ------IQIKMAQGAKPGEGGQLPGHKVSEYIAKLRVSVPGVGLISPPPHHDIYSI 1031 + Q I++K++QGAKPG GG LP K++ IA R GV ISP H + Sbjct: 227 AQAQSPQIKMIEVKLSQGAKPGHGGVLPAAKITPEIAATRGIPMGVDCISPAVHSSFSTP 286 Query: 1032 EDLAQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAK------SDHVVIAGHDGGTG 1085 L + I L+ ++ + +KL VG G+AKA D + + G +GGTG Sbjct: 287 IGLLEFIEKLRGLSGGKPVGIKLC--VGHPWEFFGIAKAMLKTDIVPDFITVDGSEGGTG 344 Query: 1086 ASPLSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGAMLGADEFG 1145 A+PL + H G P + GL TLV GLR+++++ A G++ T DV LGAD Sbjct: 345 AAPLEFVDHVGMPLQEGLVMVHNTLVGIGLRDKVKIGASGKIITAFDVARTLALGADWCN 404 Query: 1146 FATAPLVVEGCIMMRKCHLNTCPVGVATQD 1175 A + GCI + CH + CP GVATQ+ Sbjct: 405 SARGFMFALGCIQSQSCHTDRCPTGVATQN 434 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,914,988 Number of extensions: 216479 Number of successful extensions: 601 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 599 Number of HSP's successfully gapped: 2 Length of query: 1558 Length of database: 1,619,870 Length adjustment: 98 Effective length of query: 1460 Effective length of database: 1,155,840 Effective search space: 1687526400 Effective search space used: 1687526400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 137 (57.4 bits)