BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3798.p (485 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 839 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 351 7e-98 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 318 8e-88 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 247 2e-66 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 123 4e-29 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 839 bits (2167), Expect = 0.0, Method: Compositional matrix adjust. Identities = 443/485 (91%), Positives = 462/485 (95%), Gaps = 3/485 (0%) Query: 1 VGTNDNSLSDARVRMLPYIVAATFFMEYLDTTIIATALPEMAKTFGVGPNELSLGMTAYM 60 V TNDNSL+DARVRMLPYIVAATFFMEYLDTTIIATALP+MA++FGVGPNELSLGMTAYM Sbjct: 17 VATNDNSLTDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYM 76 Query: 61 LTLAVFIPISGWVADRFGSRTVFGLAIAIFTFASILCGLSQNVWEFTAARVLQGLGGAMM 120 LTLAVFIPISGWVADRFGSRTVFG+AIAIFT ASILCG SQNVWEFTAARVLQGLGGAMM Sbjct: 77 LTLAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMM 136 Query: 121 VPVGRMIVVRSTSKERLMKAIATITWPAIAAPVVGPSIGGFITTYASWHWIFLMNVPFGI 180 VPVGRMIVVRST K+RLMKAIATITWPAI APVVGPSIGGFITTYASWHWIFLMNVPFG+ Sbjct: 137 VPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGL 196 Query: 181 AAFGAVMLVVRNEHGQGKRPLDVIGFLLSGSALTSLLYGTEIASHMESNVKVALGFVVLG 240 AAF AVM+VVRNEHG KRPLD++GFLLSG+ALTSLLYGTEIASHMESN+KVALGFV LG Sbjct: 197 AAFTAVMMVVRNEHGVSKRPLDLVGFLLSGAALTSLLYGTEIASHMESNIKVALGFVALG 256 Query: 241 IVLGWCAWWHMRRIEHPLLDFSNLRIPTYSVTVFTGSLTRIGVDAVPYLMPLMFQIGFGL 300 +VLG+ AW HM+RIEHPLLDFSNLRIPTYSVTV TGSLTRIGVDAVPYLMPLMFQIGFGL Sbjct: 257 VVLGYGAWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGL 316 Query: 301 TAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVALNNAAIAGCLIIVCGLLRPGTPLP 360 TAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVA+NNAAIAG LII CGLL P TPLP Sbjct: 317 TAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLP 376 Query: 361 VILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALR 420 VILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALR Sbjct: 377 VILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALR 436 Query: 421 IAAWLRGEVVGAVDASGAHFTLGDFQWAFAMAGVVTLMSVLGYRGLAPDAGSNIGGGMRA 480 IAA LRGE A+DASGAHFTLGDFQWAFAMAGVVTL+SV+GYRGLA DAGSNIGGGMRA Sbjct: 437 IAAALRGE---AIDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAGSNIGGGMRA 493 Query: 481 VQGKR 485 GKR Sbjct: 494 APGKR 498 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 351 bits (901), Expect = 7e-98, Method: Compositional matrix adjust. Identities = 189/448 (42%), Positives = 273/448 (60%), Gaps = 9/448 (2%) Query: 29 LDTTIIATALPEMAKTFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGLAIA 88 +D+T+I+T+LP +A V P L L +T+Y+++LAVFIPISGW+ADRFGS T+F AI Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 89 IFTFASILCGLSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTSKERLMKAIATITWPA 148 +F SILCG+S + +F AAR LQG+GGAMMVPVGR++++RS K L+KA++ +T PA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 149 IAAPVVGPSIGGFITTYASWHWIFLMNVPFGIAAFGAVMLVVRNEHGQGKRPLDVIGFLL 208 + PV+GP +GGFITTY W WIF +N+P I + + + PLD+ GFLL Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 209 SGSALTSLLYGTEIASHMESNVKVALGFVVLGIVLGWCAWWHMRRIEHPLLDFSNLRIPT 268 S + L+ G A + +VAL V+ G+V WH RR PL+D + L+IPT Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 269 YSVTVFTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQ 328 VF GS R+G+ A+P+L+PLM Q+GFG T F SG+L A+A G + MK R L+ Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 329 RFGFRSVALNNAAIAGCLIIVCGLLRPGTPLPVILGVIFCYGLSRSMQFSTLATLAYADI 388 R+GFR V L N A+ + P TP +++ ++ G RS+QF++L ++YAD+ Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 389 SDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAWLRGEVVGAVDASGAHFTLGDFQWA 448 R S A+++ SVAQQ++IGMG+ AL+++ ++G A DF A Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGH---------ATLVAQDFWPA 411 Query: 449 FAMAGVVTLMSVLGYRGLAPDAGSNIGG 476 F + G V ++L R L DAG+ + G Sbjct: 412 FLVVGAVAACALLMLRQLPHDAGAEMAG 439 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 318 bits (814), Expect = 8e-88, Method: Compositional matrix adjust. Identities = 181/454 (39%), Positives = 269/454 (59%), Gaps = 19/454 (4%) Query: 29 LDTTIIATALPEMAKTFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGLAIA 88 +D T++AT+LP +A+ P L L MTAY+ L VFIPISGW+AD+ G+R VF A+ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 89 IFTFASILCGLSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTSKERLMKAIATITWPA 148 +F SILC +S ++ F AAR LQG+GGAMMVPVGR+I+ RS K L+KA++ +T PA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 149 IAAPVVGPSIGGFITTYASWHWIFLMNVPFGIAAFGAVMLVVRNEHGQGKRPLDVIGFLL 208 + PVVGP +GGFITTY W W+FL+N+PF ++N PLDV GF+L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 209 S--GSALTSL---LYGTEIASHMESNVKVALGFVVLGIVLGWCAWWHMRRIEHPLLDFSN 263 + G+A+ L L+G+ + ++G + L + + H +IE PLLDF Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMY-----YRHSTQIEFPLLDFRL 235 Query: 264 LRIPTYSVTVFTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFT 323 LRIPT +V GSL RIG+ AVP+L+PL Q G G+TAFQ+G + A+A G+L ++ Sbjct: 236 LRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLA 295 Query: 324 TRILQRFGFRSVALNNAAIAGCLIIVCGLLRPGTPLPVILGVIFCYGLSRSMQFSTLATL 383 R+L+R+GFR+V + NA AG + G+ TP +I+ ++ G+ ++QF+ L ++ Sbjct: 296 GRVLRRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSI 355 Query: 384 AYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAWLRGEVVGAVDASGAHFTLG 443 AYADI ++L SV QQ+++G+G+ + L+++ L+G A Sbjct: 356 AYADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGH---------ATIVEA 406 Query: 444 DFQWAFAMAGVVTLMSVLGYRGLAPDAGSNIGGG 477 DF AF + G+ ++ SV L +AG + G Sbjct: 407 DFWPAFLVIGLFSVASVPISGKLPHNAGLELSRG 440 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 247 bits (630), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 158/457 (34%), Positives = 251/457 (54%), Gaps = 16/457 (3%) Query: 14 RMLPYIVAATFFMEYLDTTIIATALPEMAKTFGVGPNELSLGMTAYMLTLAVFIPISGWV 73 R L +IVA+ FFM+ LDTTI+ TALP MA++ G + + AY LT+A P SGW+ Sbjct: 52 RALLWIVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWL 111 Query: 74 ADRFGSRTVFGLAIAIFTFASILCGLSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTS 133 ADRFG+R ++ AI +F+ S+LC ++ ++ AARV+QGLGG+M++PVGR+ V+R+ Sbjct: 112 ADRFGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVP 171 Query: 134 KERLMKAIATITWPAIAAPVVGPSIGGFITTYASWHWIFLMNVPFGIAAFGAVMLVVRNE 193 + + A+A+I+ PVVGP +GG++ SWHWIFL+N+P G+ GAV+ + ++ Sbjct: 172 GAQYIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDD 231 Query: 194 HGQGKRPLDVIGFLLSGSALT--SLLYGTEIASHMESNVKVALGFVVLGIVLGWCAWWHM 251 G D++G +L + + SL SH + +AL V VLG+ W H Sbjct: 232 SLAGTPRFDLLGCVLLSACMVAFSLALDVPFDSH-RTGWSIALFVVAALTVLGY--WRHA 288 Query: 252 RRIEHPLLDFSNLRIPTYSVTVFTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLA 311 PL P +S+ + + RIG AVP+L+PL+ Q+ G + +G++LL Sbjct: 289 SVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLP 348 Query: 312 TALGNLGMKAFTTRILQRFGFRSVALNNAAIAGCLIIVCGLLRPGTPLPVILGVIFCYGL 371 A+ K + +++RFG+ L N AI G I+ L+ P PL + + +G Sbjct: 349 AAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGA 408 Query: 372 SRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAWLRGEVVG 431 S SMQF+ + ++ + S ++L+S+ Q + IG+G+ G + + + EV Sbjct: 409 SNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNL---FKSEVGT 465 Query: 432 AVDASGAHFTLGDFQWAFAMAGVVTLMSVLGYRGLAP 468 A +AS FQ FA+ G +TL S +R + P Sbjct: 466 ATNAS--------FQITFAIVGAITLASAWVFRKMDP 494 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 123 bits (308), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 78/246 (31%), Positives = 132/246 (53%), Gaps = 6/246 (2%) Query: 20 VAATFFMEYLDTTIIATALPEMAKTFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGS 79 V+ FM LD++I A+P ++ GV +E + +T++ A+ IP++GW+ R G+ Sbjct: 29 VSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGA 88 Query: 80 RTVFGLAIAIFTFASILCGLSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTSKERLMK 139 +F ++ +F AS LCGL+ + AARVLQGL MVP+ + +++ + K + Sbjct: 89 VRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSF 148 Query: 140 AIATITWPAIAAPVVGPSIGGFITTYASWHWIFLMNVPFGIAAFGAVMLVVRN-EHGQGK 198 A+A A+ APV GP++GG+IT +W WIF +N+P G+ A G + + R+ E K Sbjct: 149 ALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHK 208 Query: 199 RPLDVIGFLLSGSALTSLLYGTEIASHME---SNVKVALGFVVLGIVLGWCAWWHMRRIE 255 P+D IG L + + + + ++ S V LG + L I + W + + Sbjct: 209 LPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIAL-IAFAYFVVWELNN-D 266 Query: 256 HPLLDF 261 HP++D Sbjct: 267 HPVVDL 272 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,451,513 Number of extensions: 60143 Number of successful extensions: 399 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 380 Number of HSP's successfully gapped: 18 Length of query: 485 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 396 Effective length of database: 1,198,455 Effective search space: 474588180 Effective search space used: 474588180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)