BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3787.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 459 e-131 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 183 1e-47 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 158 5e-40 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 140 9e-35 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 101 5e-23 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 459 bits (1182), Expect = e-131, Method: Compositional matrix adjust. Identities = 231/258 (89%), Positives = 247/258 (95%) Query: 1 MSYENILVETHDKVGLIRLNRPKALNALNDALMIELGQALLAFDAQDEIGCIIVTGSEKA 60 MSYENI VETH+KVGLIRL+RPKALNAL+D LM ELG+ALLAFDAQ+EIGCII+TGSEKA Sbjct: 1 MSYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKA 60 Query: 61 FAAGADISVMAGYDYMDVFNGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI 120 FAAGADIS MAGYDYMDVF GEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI Sbjct: 61 FAAGADISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI 120 Query: 121 IAADNAKFGQPEIKLGIIPGAGGTQRLPRAVSKAKAMDLALTARMMDAQEAERAGLVSRV 180 IAADNAKFGQPEIKLGI+PGAGGTQRLPRAVSKAKAMDLALT RMMDA+EAERAGLVSR+ Sbjct: 121 IAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRI 180 Query: 181 VPADKLMEEAMAAAVTITEMPRQVAMMVKDSVNRAYESTLTEGIKYERALFYSCFATEDQ 240 V ADKL+EEAMAAA+ I EMPRQVAMMVKDSVNRAYE+TL+EG+KYERALFYSCFATEDQ Sbjct: 181 VAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATEDQ 240 Query: 241 KEGMKAFLEKRPAVFKNA 258 KEGMKAFLEKR VFK++ Sbjct: 241 KEGMKAFLEKRQPVFKHS 258 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 183 bits (465), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 104/256 (40%), Positives = 153/256 (59%), Gaps = 2/256 (0%) Query: 1 MSYENILVETH-DKVGLIRLNRPKALNALNDALMIELGQALLAFDAQDEIGCIIVTGSEK 59 ++YE++L H + V + LNRP NAL + + E+ AL + D + ++++G+EK Sbjct: 5 VAYEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEK 64 Query: 60 AFAAGADISVMAGYDYMDVFNGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDF 119 AFAAGAD++ M D + + + W + R KP++AAV GYALG GCEL M D Sbjct: 65 AFAAGADLNEMIHKDAIAT-QLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADI 123 Query: 120 IIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVSKAKAMDLALTARMMDAQEAERAGLVSR 179 IAA AK GQPEI +G +PGAGGTQRL R V K AM + L+ + A +A +AGLV+ Sbjct: 124 AIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAE 183 Query: 180 VVPADKLMEEAMAAAVTITEMPRQVAMMVKDSVNRAYESTLTEGIKYERALFYSCFATED 239 VV D +E +A A +I + + K+++ +++E L G+ +ER F A+ D Sbjct: 184 VVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASAD 243 Query: 240 QKEGMKAFLEKRPAVF 255 ++EG+ AF EKR AVF Sbjct: 244 RQEGIAAFQEKRAAVF 259 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 158 bits (399), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 98/262 (37%), Positives = 144/262 (54%), Gaps = 9/262 (3%) Query: 1 MSYENILVETHDKVGLIRLNRPKALNALNDALMIELGQALLAFDAQDEIGCIIVTGSEKA 60 MS +IL+E D + +I LNRP LN+ A+ +EL A+ A + +++TG+ + Sbjct: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 Query: 61 FAAGADI---SVMAGYDYMDVFNGEFITRNW----ETLRRIRKPVIAAVAGYALGGGCEL 113 F AG D+ +V G D +D+ GE I + + ++LR + PVI AV G A G G L Sbjct: 61 FCAGQDLGDRAVKPGDDGVDL--GESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANL 118 Query: 114 AMMCDFIIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVSKAKAMDLALTARMMDAQEAER 173 + CD ++AA +A F + KLG+IP GGT LPR V A+AM +AL + A++AER Sbjct: 119 PLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAER 178 Query: 174 AGLVSRVVPADKLMEEAMAAAVTITEMPRQVAMMVKDSVNRAYESTLTEGIKYERALFYS 233 GL+ + V D+LMEEA A A P + K ++ + TL E + ER + Sbjct: 179 WGLIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRA 238 Query: 234 CFATEDQKEGMKAFLEKRPAVF 255 + D +EG+ AFLEKR F Sbjct: 239 LGRSRDYREGVTAFLEKRAPQF 260 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 140 bits (353), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 92/258 (35%), Positives = 135/258 (52%), Gaps = 3/258 (1%) Query: 2 SYENILVETHDKVGLIRLNRPKALNALNDALMIELGQALLAFDAQDEIGCIIVTG-SEKA 60 +Y N+ +E ++ L+ P A N A + +L + + +A +I ++VTG EK Sbjct: 3 TYTNLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKF 61 Query: 61 FAAGADISVMA-GYDYMDVFNGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDF 119 F+AGAD+ + A G M +ETL R IAA+ GYA+GGG E A+ CD Sbjct: 62 FSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDI 121 Query: 120 IIAADNAKFGQPEIKLGIIPGAGGTQRLPRAVSKAKAMDLALTARMMDAQEAERAGLVSR 179 IA + A+ PE +G++P AGGTQ LP V + A + L ++A+ A R GLV Sbjct: 122 RIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEE 181 Query: 180 VVPADKLMEEAMAAAVTITEMPRQVAMMVKDSVNRAYESTLTEGIKYERALFYSCFATED 239 VVP + E A+A A + K + A + L ++ ER LF F T+D Sbjct: 182 VVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQD 241 Query: 240 QKEGMKAFLEKRPAVFKN 257 Q+EG++AFLEKR +KN Sbjct: 242 QREGVQAFLEKRSPQWKN 259 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 101 bits (252), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 72/252 (28%), Positives = 117/252 (46%), Gaps = 11/252 (4%) Query: 7 LVETHDKVGL--IRLNRPKALNALNDALMIELGQALLAFDAQDEIGCIIVTGSEKAFAAG 64 ++E D+ G+ + LNRP+ NAL++ ++ L L + + C+I+ + KAF AG Sbjct: 8 ILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFCAG 67 Query: 65 ADISVMAGYDYMDVFNGEF--ITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIA 122 D+ M + F + ++LR + PVIA V G A GC+L CD IA Sbjct: 68 HDLRQMQATPQQTYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATCDLAIA 127 Query: 123 ADNAKFGQPEIKLGII---PGAGGTQRLPRAVSKAKAMDLALTARMMDAQEAERAGLVSR 179 A++A+F I +G+ P T+ LP +A ++ +T + +DA A GL+++ Sbjct: 128 AESARFAVSGINVGLFCSTPAVALTRNLP----IKRAFEMLVTGKFIDAATAAAWGLINQ 183 Query: 180 VVPADKLMEEAMAAAVTITEMPRQVAMMVKDSVNRAYESTLTEGIKYERALFYSCFATED 239 VP +L A A I P K V R L + + + + Sbjct: 184 AVPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMDPE 243 Query: 240 QKEGMKAFLEKR 251 +EG+ AFL+KR Sbjct: 244 AQEGITAFLDKR 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 757,744 Number of extensions: 30318 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 10 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)