BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3782.p (511 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspiri... 1024 0.0 gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herb... 281 1e-76 >gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspirillum seropedicae SmR1] Length = 511 Score = 1024 bits (2647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 495/511 (96%), Positives = 502/511 (98%) Query: 1 MKATSPVIRLNPVDDVVIARQQLISGTVLQEEGGLKVMGLIPAGHKMATRAIAAGEAVKR 60 MKA SPVIRLNPVDDVVIARQQLISGTVLQ+EGGLKV GLIPAGHKMATRAI AGE VKR Sbjct: 1 MKAHSPVIRLNPVDDVVIARQQLISGTVLQDEGGLKVQGLIPAGHKMATRAITAGEPVKR 60 Query: 61 YGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVAEPATFMGIVRPDGRV 120 YGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVA PATFMGIVRPDGRV Sbjct: 61 YGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVATPATFMGIVRPDGRV 120 Query: 121 ATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLAAYPNIDGVVALTHGQGCATDSQGE 180 ATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLA YPNIDGVVALTHGQGCA DSQGE Sbjct: 121 ATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLADYPNIDGVVALTHGQGCALDSQGE 180 Query: 181 PLQILRRTLAGYATHPNFAAVLVVGLGCETNQISGLMESHNLKEGEYFHTFTIQGTGGTA 240 PLQILRRTLAGYATHPNFAAVLVVGLGCETNQISGLMESHNLKEGEYFHTFTIQGTGGTA Sbjct: 181 PLQILRRTLAGYATHPNFAAVLVVGLGCETNQISGLMESHNLKEGEYFHTFTIQGTGGTA 240 Query: 241 KTVALGIEKIKKMLPKANDIQRQPVSAKHLTLGLQCGGSDGYSGITANPALGAAVDLLVR 300 KTVALGIEKIKKMLPKANDI+R+PVSAKHLTLGLQCGGSDGYSGITANPALGAAVDLLVR Sbjct: 241 KTVALGIEKIKKMLPKANDIKREPVSAKHLTLGLQCGGSDGYSGITANPALGAAVDLLVR 300 Query: 301 HGGTAILSETPEIYGAEHLLTRRAVSPEVGQKLLARIAWWEGYCAKNDAEMNNNPSAGNK 360 HGGTAILSETPEIYGAEHLLTRRAVSPEVG+KLLARIAWWE YCAKNDAEMNNNPSAGNK Sbjct: 301 HGGTAILSETPEIYGAEHLLTRRAVSPEVGEKLLARIAWWEEYCAKNDAEMNNNPSAGNK 360 Query: 361 AGGLTTILEKSLGAVAKGGTTNLVDVYQYAETVTARGFVFMDTPGYDPISATGQVAGGAN 420 AGGLTTILEKSLGAVAKGGTTNLVDVY+YAE VTARGFVFMDTPGYDPISATGQVAGGAN Sbjct: 361 AGGLTTILEKSLGAVAKGGTTNLVDVYKYAEAVTARGFVFMDTPGYDPISATGQVAGGAN 420 Query: 421 VICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINCGEIADGDVTPQEIGERFFQM 480 +ICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINCGEIADG+VTPQEIGERFFQM Sbjct: 421 MICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINCGEIADGNVTPQEIGERFFQM 480 Query: 481 ILDTASGKKTKSELHGYGQDEFVPWHIGVYT 511 ILDTASGKKTKSELHGYGQDEFVPWHIGVYT Sbjct: 481 ILDTASGKKTKSELHGYGQDEFVPWHIGVYT 511 >gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herbaspirillum seropedicae SmR1] Length = 522 Score = 281 bits (719), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 181/503 (35%), Positives = 269/503 (53%), Gaps = 26/503 (5%) Query: 2 KATSP-VIRLNPVDDVVIARQQ--LISGTVLQEEGGLKVMGLIPAGHKMATRAIAAGEAV 58 +A++P I +N D+V I L +GTV + GL ++ +P GHK+A R + GEA+ Sbjct: 9 QASAPRYIMMNDTDNVAIVVNDGGLPAGTVFPD--GLTLVDRVPQGHKIALRDLKQGEAI 66 Query: 59 KRYGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFG-ADVKPV-DFVAEPATFMGIVRP 116 RY IG A +DI G + + M + A KP E TF G Sbjct: 67 VRYDVAIGYAVRDIPKGGWIEESLVQMPPARELDNLPIATKKPAPQPPLEGYTFEGYRNA 126 Query: 117 DGRVATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLAAYPNIDGVVALTHGQGCATD 176 DG V TRN + + T+V C A + ++ + I E+L YPN++ VVAL H GC Sbjct: 127 DGSVGTRNLLAITTTVQCVA----GVVEHAVKRIRAELLPKYPNVEDVVALEHTYGCGVA 182 Query: 177 SQGEPLQILRRTLAGYATHPNFAA-VLVVGLGCETNQISGLMESHNL---KEGEYFHTFT 232 I RTL + +PNF +VV LGCE Q + L+ + + K+GE + Sbjct: 183 IDAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHKQGEPY-VVC 241 Query: 233 IQGTGGTAKTVALG--IEKIKKM----LPKANDIQRQPVSAKHLTLGLQCGGSDGYSGIT 286 +Q A+ V I+ I M L + N +R+ A L +G+QCGGSD +SG+T Sbjct: 242 LQ----DAEHVGFNSMIDSIMNMAEARLTELNKRRRETCPASDLVVGVQCGGSDAFSGVT 297 Query: 287 ANPALGAAVDLLVRHGGTAILSETPEIYGAEHLLTRRAVSPEVGQKLLARIAWWEGYCAK 346 ANPA+G A DLLVR G + + SE E+ LT RAV+ EV Q ++ + W++ Y + Sbjct: 298 ANPAVGFATDLLVRAGASVMFSEVTEVRDGIDQLTSRAVNEEVAQAMIREMDWYDNYLKQ 357 Query: 347 NDAEMNNNPSAGNKAGGLTTILEKSLGAVAKGGTTNLVDVYQYAETVTARGFVFMDTPGY 406 + + N + GNK GGL I+EK++G++ K G++ + V + + +G ++ TP Sbjct: 358 GGVDRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVLSPGDKLQQKGLIYAATPAS 417 Query: 407 DPISATGQVAGGANVICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINCGEIADG 466 D I T Q+A G N+ FTTGRG+ YG A P +K+AT L +R + MD+N G IA G Sbjct: 418 DFICGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNAGRIASG 477 Query: 467 DVTPQEIGERFFQMILDTASGKK 489 + + +++G FQ++LD ASGKK Sbjct: 478 EASIEDVGWELFQLMLDVASGKK 500 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,769,393 Number of extensions: 81262 Number of successful extensions: 252 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 248 Number of HSP's successfully gapped: 2 Length of query: 511 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 422 Effective length of database: 1,198,455 Effective search space: 505748010 Effective search space used: 505748010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)