BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3771.p (397 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 449 e-127 gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 372 e-104 gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 370 e-103 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 449 bits (1154), Expect = e-127, Method: Compositional matrix adjust. Identities = 219/387 (56%), Positives = 269/387 (69%), Gaps = 5/387 (1%) Query: 11 QSELQQIRRDIHAHPELAYEEVRTADVVAQKLTEWGIPVVRGLGVTGVVGIIKQGDSPRA 70 + +L IRR +HAHPEL +EE RTA VA L +G+ V +G GVV +++G S RA Sbjct: 14 EMDLVSIRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATLRKGKSTRA 73 Query: 71 IGLRADMDALPMPEINTFAHASRHAGKMHACGHDGHTAMLLGAAYYLARHRNFDGTVYVI 130 I LRADMDALPM E N F+H S AGKMHACGHDGHT MLLGAA L+R FDGTV+ + Sbjct: 74 IALRADMDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRLSREVEFDGTVHFV 133 Query: 131 FQPAEEGGRGAERMIQDGLFEQYPMDAVFGMHNWPGIPAGHFGVTPGAQMASSNEFHVTV 190 FQPAEEGG GA MI+DGLFE++P DA+FG+HNWPG+PAG FG+ PG MASSN F T+ Sbjct: 134 FQPAEEGGAGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPGPLMASSNTFSATL 193 Query: 191 KGRGSHAAQPHKAADPVMTAVHIAQAWQSIVARNINPNDPAVVSITQIHTGSATNVIPDE 250 GRG+H AQPH++ DPV+ A + AWQSIV RNINPN AV+S+TQ+HTG+A NVIP++ Sbjct: 194 FGRGAHGAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQLHTGTADNVIPEQ 253 Query: 251 AMMVGTVRTFSLPVLDLIERRMKEIAEHTAAAFDATVDFRFNRNYPPLINHAKETAFAVE 310 A + GTVR+F LDLIE RM IAE FD V F F R YP ++NH + T A++ Sbjct: 254 ATLSGTVRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPAVVNHPEATQQAMQ 313 Query: 311 VLTEQFGADRVDAHTEPTMGAEDFAFMLQHKPGCYVFLGNG---DGGHRDQGHGLGPCNL 367 + GA++VD TEP +EDFAF LQHKPGCY FLGNG DG + G L Sbjct: 314 AMMAAVGAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADG--KQAGSQATSREL 371 Query: 368 HNPSYDFNDDLLPIGATYWVRLAEKFL 394 H+P YDFNDD++ G YWV L ++L Sbjct: 372 HSPFYDFNDDIIDAGVAYWVSLVRQYL 398 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 372 bits (956), Expect = e-104, Method: Compositional matrix adjust. Identities = 187/387 (48%), Positives = 254/387 (65%), Gaps = 12/387 (3%) Query: 10 FQSELQQIRRDIHAHPELAYEEVRTADVVAQKLTEWGIPVVRGLGVTGVVGIIKQGDSPR 69 Q E+ IRR +H HPEL++EEV TA +VAQ+L +WG V R +G G+V ++ G S R Sbjct: 18 IQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGNGLVATLRVGSSAR 77 Query: 70 AIGLRADMDALPMPEINTFAHASRHAGKMHACGHDGHTAMLLGAAYYLARHRNFDGTVYV 129 +IGLRADMDALP+ E AS G MHACGHDGHTAMLLGAA +LAR R FDGT+ + Sbjct: 78 SIGLRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLGAARHLARTRRFDGTLNL 137 Query: 130 IFQPAEEGG--RGAERMIQDGLFEQYPMDAVFGMHNWPGIPAGHFGVTPGAQMASSNEFH 187 IFQPAEE G GA++M+ DGLFE++P +AVFG+HN PG+ AG F G MA+ + Sbjct: 138 IFQPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMFRSGPFMAACDTVK 197 Query: 188 VTVKGRGSHAAQPHKAADPVMTAVHIAQAWQSIVARNINPNDPAVVSITQIHTGSATNVI 247 + + GRGSHAA+PH + DPV+ A + A Q++V+RNI+P D AVV++ +H G A+NVI Sbjct: 198 IRITGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDSAVVTVGSLHAGKASNVI 257 Query: 248 PDEAMMVGTVRTFSLPVLDLIERRMKEIAEHTAAAFDATVDFRFNRNYPPLINHAKETAF 307 P+ A M +VR+F V +L+E+R++ + A ++ A + + R YP L+N ET F Sbjct: 258 PEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEIDYLRGYPVLVNSDAETDF 317 Query: 308 AVEVLTEQFGADRVDAHTEPTMGAEDFAFMLQHKPGCYVFLGNGDGGHRDQGHGLGPCNL 367 A V E G ++V A P G+EDFA+ L+ +PGC++ +GNG G L Sbjct: 318 ARSVAEELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGNGQ----------GKPML 367 Query: 368 HNPSYDFNDDLLPIGATYWVRLAEKFL 394 HN YDFND +PIGA YW RL E+++ Sbjct: 368 HNAGYDFNDANIPIGAAYWTRLVERWM 394 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 370 bits (949), Expect = e-103, Method: Compositional matrix adjust. Identities = 185/390 (47%), Positives = 242/390 (62%), Gaps = 7/390 (1%) Query: 5 DPIVQFQSELQQIRRDIHAHPELAYEEVRTADVVAQKLTEWGIPVVRGLGVTGVVGIIKQ 64 D + Q ++ +RRD+HAHPEL +EE RTADVV ++L G V RGLG TGVV + Sbjct: 9 DTLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASLAG 68 Query: 65 GDSPRAIGLRADMDALPMPEINTFAHASRHAGKMHACGHDGHTAMLLGAAYYLARHRNFD 124 D R I LRAD+DALP+ E N FAHAS G MHACGHDGHT MLLGAA L + Sbjct: 69 ADPQRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLP 128 Query: 125 GTVYVIFQPAEEGGRGAERMIQDGLFEQYPMDAVFGMHNWPGIPAGHFGVTPGAQMASSN 184 G+V+ +FQP EEGG GA +MI DGLFEQ+P +AVFGMHNWPG+PAG FG+ PG MA+ + Sbjct: 129 GSVHFVFQPGEEGGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRPGPIMAAGS 188 Query: 185 EFHVTVKGRGSHAAQPHKAADPVMTAVHIAQAWQSIVARNINPNDPAVVSITQIHTGSAT 244 +T+ G+G+HAAQPH DP+ A + Q+I AR+ +P DPAV+S+ H G Sbjct: 189 RLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTD 248 Query: 245 NVIPDEAMMVGTVRTFSLPVLDLIERRMKEIAEHTAAAFDATVDFRFNRNYPPLINHAKE 304 NVIPD A + GT+RT S + ++ ++ + A A+ A V+ F + YP IN E Sbjct: 249 NVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQYYPATINTPAE 308 Query: 305 TAFAVEVLTEQFGADRVDAHTEPTMGAEDFAFMLQHKPGCYVFLGNGDGGHRDQGHGLGP 364 T F V+ + FG DR+ M +EDF FML+ +PG YV +GN Sbjct: 309 TDFCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASH------ 362 Query: 365 CNLHNPSYDFNDDLLPIGATYWVRLAEKFL 394 +LH+P YDFNDD++ G YWV LA+ + Sbjct: 363 -SLHHPHYDFNDDIIEAGVRYWVALAQGYF 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,344,235 Number of extensions: 59226 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 130 Number of HSP's successfully gapped: 3 Length of query: 397 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 310 Effective length of database: 1,207,925 Effective search space: 374456750 Effective search space used: 374456750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)