BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3770.p (210 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313160|ref|YP_003777252.1| threonine efflux protein [Herba... 230 8e-62 >gi|300313160|ref|YP_003777252.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 207 Score = 230 bits (586), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 140/191 (73%), Positives = 160/191 (83%), Gaps = 3/191 (1%) Query: 1 MFSSEFLLLAGAHLLALASPGPDFLLLVRSSLRHGRGYALAASLGIALANGIYIALAIGG 60 MFSSEFLLLA AHLLALASPGPDFLLLVR +LRHGRGY LAAS GIA ANG+YIALAI G Sbjct: 1 MFSSEFLLLASAHLLALASPGPDFLLLVRGALRHGRGYGLAASAGIAAANGLYIALAIAG 60 Query: 61 FSVLQENPSLLLAMQWLAALYLAWLGWLFLRASGQPADLCAHLSGGQRVRVSPLGGLGAG 120 FS+LQ +P+LL+AMQWLA+LYLAWLGW+F+RAS +PA S G++ + G+GAG Sbjct: 61 FSLLQHSPALLVAMQWLASLYLAWLGWMFMRASLEPAPWPG--SAGRQAAAA-WRGVGAG 117 Query: 121 FLSAALNPKNGLFYFGLFTVIVAAHTGLQRKLFYGLWMFAAVLAWDAGLVCLIAQRRVVQ 180 FLSA LNPKNGLFYFGLFT++V AHTGL RKLFYG+WMF AVLAWDAGLVCL+AQ RV Sbjct: 118 FLSALLNPKNGLFYFGLFTLMVEAHTGLPRKLFYGVWMFGAVLAWDAGLVCLMAQPRVAA 177 Query: 181 ALTRLLPLIER 191 L R LP++ER Sbjct: 178 LLDRALPMVER 188 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 488,587 Number of extensions: 15884 Number of successful extensions: 97 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 3 Length of query: 210 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 129 Effective length of database: 1,236,335 Effective search space: 159487215 Effective search space used: 159487215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)