BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3738.p (373 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxido... 752 0.0 gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase la... 75 1e-14 gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase... 66 4e-12 >gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 374 Score = 752 bits (1942), Expect = 0.0, Method: Compositional matrix adjust. Identities = 355/372 (95%), Positives = 365/372 (98%) Query: 1 MSDLATFAKLARSNAQLPVHVYFDEPLYQRELKQLFQHGPRYVGHELMVPNAGDYSTLPW 60 MSDLATFAKLARSNAQLPVHVYFDEPLYQRELKQLFQ GPRYVGHELMVP AGDYSTLPW Sbjct: 1 MSDLATFAKLARSNAQLPVHVYFDEPLYQRELKQLFQQGPRYVGHELMVPEAGDYSTLPW 60 Query: 61 ENEGRMLVRNAQGIELLSNVCRHRQAKMLDGRGNTNNVVCPLHRWTYDLKGELIGAPHFG 120 ENEGRMLVRNAQGIEL+SNVCRHRQAKMLDGRG+ +N+VCPLHRWTYDLKGELIGAPHFG Sbjct: 61 ENEGRMLVRNAQGIELVSNVCRHRQAKMLDGRGHASNIVCPLHRWTYDLKGELIGAPHFG 120 Query: 121 ETPCMNLSKTPLQSWNGLLFENNGVDIADKLKRLGVTRDLDFSGYLYDHTEVHECDYNWK 180 +TPCMNLSKTPLQSWNGLLFE NGVDIADKLK LGVTRDLDFSGYLYDHTEVHECDYNWK Sbjct: 121 QTPCMNLSKTPLQSWNGLLFEANGVDIADKLKNLGVTRDLDFSGYLYDHTEVHECDYNWK 180 Query: 181 TFIEVYLEDYHVEPFHPGLGNFVSCSDLKWEFGRDYSVQTVGVNHGLIKSGSPTYGKWQE 240 TFIEVYLEDYHVEPFHPGLGNFVSCSDLKWEFGRDYSVQTVGVNHGLIKSGSPTY KWQE Sbjct: 181 TFIEVYLEDYHVEPFHPGLGNFVSCSDLKWEFGRDYSVQTVGVNHGLIKSGSPTYAKWQE 240 Query: 241 KLLQFSNGQPPKFGAIWLTLYPNIMVEWYPHVLVVSTLWPSGPQKTTNVVEFYYPEEIAL 300 KLLQFSNG+PPKFGAIWLTLYPNIMVEWYPHVLVVSTLWP+GPQKTTNVVEFYYPEEIAL Sbjct: 241 KLLQFSNGKPPKFGAIWLTLYPNIMVEWYPHVLVVSTLWPTGPQKTTNVVEFYYPEEIAL 300 Query: 301 FERELVEAERAAYMETCVEDDEIAQRMDAGRRILMDRGTSEVGPYQSPMEDGMQHFHEWY 360 FERELVEAERAAYMETCVEDDEIA RMDAGRRILMDRGTSEVGPYQSPMEDGMQHFHEWY Sbjct: 301 FERELVEAERAAYMETCVEDDEIALRMDAGRRILMDRGTSEVGPYQSPMEDGMQHFHEWY 360 Query: 361 RQQLDVPPGTST 372 R+QL+VPPGT+T Sbjct: 361 RKQLEVPPGTAT 372 >gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase large terminal subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 61/219 (27%), Positives = 104/219 (47%), Gaps = 20/219 (9%) Query: 5 ATFAKLARSNAQLPVHVYFDEPLYQRELKQLFQHGPRYVGHELMVPNAGDYSTLPWENEG 64 +++A L + +A + +Y D L++ E+ ++F +V HE + N GD+ T + Sbjct: 14 SSYADLVQ-DAVVDPSLYTDPALFEAEMDKIFYKTWIWVAHESEIKNPGDFKTAQIGRQP 72 Query: 65 RMLVRNAQG-IELLSNVCRHRQAKMLDG-RGNTNNVVCPLHRWTYDLKGELIGAPH-FGE 121 ++VR+ G I +L N CRHR A + + +GN CP H W+Y L G+L G P+ G Sbjct: 73 VIVVRDKTGKINVLENRCRHRGATVCEKHKGNATGFTCPYHSWSYGLDGKLRGLPYPEGY 132 Query: 122 TPCMNLSKTPLQ-----SWNGLLFENNGVDIADKLKRLGVTRD-LDF-----SGYLYDHT 170 + + PLQ S++G++F + +I LG + +D +G+ Sbjct: 133 EDVIEKADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLFMKQGAGFPIKVQ 192 Query: 171 EVHECDY--NWKTFIEVYLEDYHVEPFHPGLGNFVSCSD 207 H+ + NWK +E + YH H F+S D Sbjct: 193 GEHKFSFKGNWKIQLENTTDGYHFPVVHK---TFLSSVD 228 >gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 462 Score = 66.2 bits (160), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 64/265 (24%), Positives = 109/265 (41%), Gaps = 44/265 (16%) Query: 21 VYFDEPLYQRELKQLFQHGPRYVGHELMVPNAGDYSTLPWENEGRMLVRNAQG-IELLSN 79 ++ + L++ E+K LF+ Y+ HE +P DY T + ++ R+ G + + N Sbjct: 36 IFTNPDLFELEMKHLFESNWVYLAHESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVIN 95 Query: 80 VCRHRQAKMLDGR-GNTNNVVCPLHRWTYDLKGELIGAP---------HFGETPCMNLSK 129 C H+ A + + GN ++ CP H WT+ G+L+ F + +L++ Sbjct: 96 ACSHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLTR 155 Query: 130 TP-LQSWNGLLFENNGVDIADKLKRLGVTRDL----------------DFSGYLYDHTEV 172 QS+ G LF + D+ + LG TR + S YLYD Sbjct: 156 VARFQSYRGFLFGSLSEDVLPLEEYLGETRVIIDQIVDQAPNGLEVLRGNSSYLYDG--- 212 Query: 173 HECDYNWKTFIEVYLEDYHVEPFHPGLGNFVSCSDLKWEFGRDYSVQTVGVNHGLIKSGS 232 NWK +E + YHV H N+ + + E G + N+ K+ + Sbjct: 213 -----NWKMQMENGCDGYHVSSVH---WNYAATMSRRKEGG----TKATDANN-WSKAVA 259 Query: 233 PTYGKWQEKLLQFSNGQPPKFGAIW 257 YG +L ++N P+ IW Sbjct: 260 GVYGFENGHILLWTNTLNPEVRPIW 284 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.138 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,305,994 Number of extensions: 57517 Number of successful extensions: 122 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 120 Number of HSP's successfully gapped: 3 Length of query: 373 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 286 Effective length of database: 1,207,925 Effective search space: 345466550 Effective search space used: 345466550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)