BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3730.p (362 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313120|ref|YP_003777212.1| glycolate oxidase iron-sulfur s... 650 0.0 gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbas... 69 5e-13 gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas... 56 6e-09 >gi|300313120|ref|YP_003777212.1| glycolate oxidase iron-sulfur subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 650 bits (1678), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/362 (89%), Positives = 342/362 (94%), Gaps = 3/362 (0%) Query: 1 MQEQAQQAQQDPETVLVSFRERILAGRPLQIRGGGTKDWYGQTPSGEVLDTRAYRGIVDY 60 MQEQAQQ D + VL SFRERILAGRPLQIRGGGTKDWYGQTPSGE+LDTRAY GI+DY Sbjct: 1 MQEQAQQ---DLDAVLASFRERILAGRPLQIRGGGTKDWYGQTPSGELLDTRAYSGIIDY 57 Query: 61 EPTELVITARCGTPLSEIDAALAEHNQMLAWEPPRFGGAATVGGMVAAGLSGPARASAGA 120 EPTELVITARCGTPLSEIDAALA+HNQMLAWEPP FGGAATVGGMVAAGLSGPARASAGA Sbjct: 58 EPTELVITARCGTPLSEIDAALAQHNQMLAWEPPSFGGAATVGGMVAAGLSGPARASAGA 117 Query: 121 VRDFVLGAVLMDAQGEQLHFGGQVMKNVAGYDIARLLAGSMGTLGLILQVSLKVLPRPFA 180 VRDFVLGAVLMDAQGE LHFGGQVMKNVAGYDI+RLLAGS+GTLGLIL+VSLKVLPRP A Sbjct: 118 VRDFVLGAVLMDAQGEPLHFGGQVMKNVAGYDISRLLAGSLGTLGLILEVSLKVLPRPIA 177 Query: 181 SSTRVFEINQEGALRLLNQSAGQPLPLVASAWLDNVLTIRLAGAQAAVDSAIAKLGGTEL 240 SSTRVFEINQEGALRLLNQSAGQPLPLVASAW+ NVLTIRL+GAQAAVDSAIAKLGGTEL Sbjct: 178 SSTRVFEINQEGALRLLNQSAGQPLPLVASAWVGNVLTIRLSGAQAAVDSAIAKLGGTEL 237 Query: 241 IHSEDVWTDLREQTHPFFADPQAPLWRLSVPSVAPPIDLPGRQLIEWGGAQRWLRPDSRL 300 + +D WTDLREQTHPFFAD +APLWRLS+PSVA PIDLPGRQLIEWGGAQRWLRP+ + Sbjct: 238 SNPDDYWTDLREQTHPFFADAEAPLWRLSLPSVAAPIDLPGRQLIEWGGAQRWLRPEGNV 297 Query: 301 DAVSIRAAALAVGGHASLHRGGDKAIGVFHPLQPAVEKIHRNLKQQFDPRGIFNPGRLYA 360 DAV+IRAAALAVGGHASLHRGGDKAIGVFHPLQPAVE +HR LKQQFDP+GIFNPGRLYA Sbjct: 298 DAVTIRAAALAVGGHASLHRGGDKAIGVFHPLQPAVETLHRRLKQQFDPQGIFNPGRLYA 357 Query: 361 SL 362 SL Sbjct: 358 SL 359 >gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 68.9 bits (167), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 93/398 (23%), Positives = 156/398 (39%), Gaps = 81/398 (20%) Query: 24 LAGRPLQIRGGGTKDWYGQTPSGEVLDTRAYRGIVDYEPTELVITARCGTPLSEIDAALA 83 L G L ++GG T D + +VL A +L T + G +++ + Sbjct: 87 LEGHVLALQGGVTVDL---SQMNQVLAVHA---------EDLTATVQAGVTRKQLNQEIK 134 Query: 84 EHNQMLAWEPPRFGGAATVGGMVAAGLSGPARASAGAVRDFVLGAVLMDAQGEQLHFGGQ 143 + +P G A++GGM + SG G +++ L ++ AQGE + G + Sbjct: 135 DTGLFFPIDP---GADASLGGMASTRASGTNAVRYGTMKENTLTLTVVTAQGEIIKTGTR 191 Query: 144 VMKNVAGYDIARLLAGSMGTLGLILQVSLKVLPRPFASSTRVFEI-NQEGALRLLNQSAG 202 K+ AGYD+ R+ GS GTLG+I +V++++ P+P A S + A+ + Q+ Sbjct: 192 AKKSSAGYDLTRVYVGSEGTLGIITEVTVRLYPQPEAISAAICSFPTVADAVNTVIQTIQ 251 Query: 203 QPLPLVASAWLDN------------------VLTIRLAGA------QAAVDSAIAK---- 234 +PL LD +L G+ QA + IAK Sbjct: 252 MGVPLARVELLDENGVRAINAHDKLSLPVNPLLLFEFHGSENGVKEQAELVQDIAKEFHA 311 Query: 235 LGGTELIHSED---VWT----------DLREQTHPFFADPQAPLWRLSVPSVAPPIDLPG 281 LG ED +WT LR D P+ RL+ +A D Sbjct: 312 LGFEWATRPEDRTRLWTARHNAYFALLQLRPGARAISTDCCVPISRLAECVLATKADCEE 371 Query: 282 RQLIEW---------GGAQRWLRPD-----SRLDAVSIRAAALAVGGHASLHRGGDKAIG 327 + L Q + P+ +R + V+ R A A+G + G+ +G Sbjct: 372 QGLTHAIIGHVGDGNFHVQMMVDPNDPADIARAEGVNQRMVARAIGMDGTC--TGEHGVG 429 Query: 328 VFHPLQPAVEK-------IHRNLKQQFDPRGIFNPGRL 358 H + +++ + R +K DP+ I NPG++ Sbjct: 430 -LHKMDFLIQEHGEGAIAVMRAIKHALDPKNIMNPGKI 466 >gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 55.8 bits (133), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 48/164 (29%), Positives = 70/164 (42%), Gaps = 13/164 (7%) Query: 28 PLQIRGGGTKDWYGQTPSGE----VLDTRAYRGIVDYEPTELVITARCGTPLSEIDAALA 83 PL +GG T G P + VL R I + IT G L + A Sbjct: 71 PLVPQGGNTGLVLGSVPDQQGNAVVLSLRRLNRIRAVDALNNTITVESGCVLQHLQEQAA 130 Query: 84 EHNQM----LAWEPPRFGGAATVGGMVAAGLSGPARASAGAVRDFVLGAVLMDAQGEQLH 139 ++ LA E G+ T+GG ++ G G R+ LG ++ AQGE + Sbjct: 131 AAGRLFPLSLAAE-----GSCTIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQGEIMS 185 Query: 140 FGGQVMKNVAGYDIARLLAGSMGTLGLILQVSLKVLPRPFASST 183 + K+ GYD+ L G+ GTLG+I +K+ P+P A T Sbjct: 186 SLKGLRKDNTGYDLRDLFIGAEGTLGVITAAVMKLFPQPRAQVT 229 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,238,346 Number of extensions: 55356 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 205 Number of HSP's successfully gapped: 3 Length of query: 362 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 276 Effective length of database: 1,212,660 Effective search space: 334694160 Effective search space used: 334694160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)