BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3728.p (709 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (... 597 e-171 gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbas... 77 3e-15 >gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (phosphatidylcholine choline phospho hydrolase) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 696 Score = 597 bits (1538), Expect = e-171, Method: Compositional matrix adjust. Identities = 337/710 (47%), Positives = 435/710 (61%), Gaps = 39/710 (5%) Query: 4 HSRRDFLRFSATLAGAASATAMLPASIRKAMAIAPQGRTGSIEDVEHIVVFMQENRSFDH 63 RR FL+ +A AA A LPASIRKA+A+AP TG+I+DV+H+V+ MQENRSFDH Sbjct: 6 QDRRHFLKSAARTGSAALALTALPASIRKALAVAPASPTGTIKDVKHVVILMQENRSFDH 65 Query: 64 YLGHLSGVRGYNDRFPVTLPNGRPVWFQPRQEDASALIAPFRYDTTDPAMNAQCVGSLSH 123 Y G L GVRG+ D P LP+G PV+ Q ++ L+ P+R + + + G+L H Sbjct: 66 YFGCLRGVRGFADPIPRPLPDGSPVFHQ--RDGQGRLVLPYRMEMATTSA-GRAGGNLPH 122 Query: 124 TWATTHGALNAGRADQWAQ-QKSNMCMGYHVREDIPFHYALADAFTVCDQYFCSIPGNTH 182 W H A N G D+W + +K + M Y RED+PFHYALADAFT+CD Y CS+ G+T+ Sbjct: 123 AWKDQHAAWNRGDYDKWIEAKKDTVTMAYFTREDLPFHYALADAFTLCDAYHCSVLGSTN 182 Query: 183 PNRMYLMTGMVDPLGTGGGPLLDNTDYIDNQFSHVKLP--------PFTWTTYPERLERA 234 PNR +LM+G +D G GGP+ + S +P F WTTYPERLE+ Sbjct: 183 PNRFHLMSGGIDLQGRAGGPMT----FQPRTGSCSGVPGERRSGADSFGWTTYPERLEQH 238 Query: 235 GISWQVYQQGTDFDKFSGNYGT--NMLASF-ENFVHAPIGSSLYERGMSTRTIAQLKADV 291 G+SW+VYQ + K G+Y + N+L F + +A S L+++ S T+A L DV Sbjct: 239 GVSWKVYQGTEELHKDHGDYPSDFNVLQYFFAQYQNAAPDSPLWQKACSKFTLADLARDV 298 Query: 292 EQGSLPQVSWLLPPAVYSEHPDYTPLYGASYLSDILDALTANPEVWSKTVLFVMYDENDG 351 G LPQVSWL+PP + EHPD TP YGA Y+S +LD LTANP +WS TVL V YDENDG Sbjct: 299 NSGKLPQVSWLMPPKLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNYDENDG 358 Query: 352 FFDHIVPPQAPTLPGSGKSTVDIALERHVHVTPRQQGQF--SADQLPYGLGPRVPMFVVS 409 FFDH+VPP P G G STV+ + E H ++G + AD LP GLG RVPM V+S Sbjct: 359 FFDHVVPPTPPLHAGQGLSTVETSAEFH------RKGDYVNPADDLPAGLGARVPMMVIS 412 Query: 410 PWSRGGRVNSQVFDHTSVLQFIERRFGVKEPNISPWRRAVCGDLSSTLDFTRRDASPPRL 469 PWS+GG V S+VFDHTSV++F+E+ F V EPNISPWRRAVCGDL+S DF+RRDA+ P L Sbjct: 413 PWSKGGYVCSEVFDHTSVIRFLEQVFDVHEPNISPWRRAVCGDLTSAFDFSRRDAASPVL 472 Query: 470 PDTRHYVADADQQCRRPVAISAPALDAALSMTPQEPGLRPACALPYALHVTGWLDAQGYT 529 PDT Y A +D Q R P AP ++ +M QE G+RPA ALPY L V + Sbjct: 473 PDTSAYRALSDAQARLP----APVAPSSPAMPQQERGMRPARALPYVLAVDDRVVPDQDA 528 Query: 530 LTLD--NVGTQGAHFWVYTDAQGAHAAAAAPRGYTVEAGKQLRDRW-ALSAEGHYHLSVW 586 L LD N G G + VY A + + +TV+ GK L +W + G Y L V+ Sbjct: 529 LQLDFLNPGKAGVVYQVY-----ARQESERYQTFTVQPGKSLTAQWRGVGPVGGYDLKVY 583 Query: 587 GPNGYFRRFVGQVAAGSLAEPDLRTHYDAAAGVLTVTLLNPGTMPLTATVSDLAYGQPAR 646 GPNG+ R G + G EP ++ +D G L + LLNPG L + D A+GQ R Sbjct: 584 GPNGFLRELRGSLERGGGVEPVVQARHDGERGELVLALLNPGRQALHLVLRDQAHGQATR 643 Query: 647 TVRVLPGQSVSSRWELAASHHWYDLQWRIAEAPHWLRRLAGHLETGHPSM 696 TV + PGQS + RW LA+SHHWYDL + + P + RRLAGH+ETG PS Sbjct: 644 TVTLAPGQSATERWPLASSHHWYDLLVQAEDRPAFARRLAGHVETGRPSF 693 >gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbaspirillum seropedicae SmR1] Length = 558 Score = 77.4 bits (189), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 62/230 (26%), Positives = 100/230 (43%), Gaps = 55/230 (23%) Query: 229 ERLERAGISWQVYQ---QGTDFDKFSGNYGTNMLASFENFVHAPIG-------SSLYERG 278 ++L + GI W Y Q T D+F + G + +F+ + H P + ER Sbjct: 335 DKLNKKGIDWAWYAGAWQAT-LDEFKDSKGIPKIPNFQ-YHHQPFNYFKNLGPENPAERN 392 Query: 279 MSTRT--------IAQLKADVEQGSLPQVSWLLPPAVYSEHPDYTPL-YGASYLSDILDA 329 R + ADVE G LP V++ P + H Y + G +++ I+ + Sbjct: 393 KRLRDGGLGDEARTNRFLADVEAGRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHIIKS 452 Query: 330 LTANPEVWSKTVLFVMYDENDGFFDHIVPPQAPTLPGSGKSTVDIALERHVHVTPRQQGQ 389 L A+P+ W V+ V +DEN G++DH+ PP+ Sbjct: 453 LQASPQ-WKNMVVVVTFDENGGWWDHVAPPKGDR-------------------------- 485 Query: 390 FSADQLPYGLGPRVPMFVVSPWSRGGRVNSQVFDHTSVLQFIERRFGVKE 439 +G G RVP VVSP++R G V+ V+D S+L+ I R F +++ Sbjct: 486 -------WGPGTRVPALVVSPFARKGTVDHTVYDTASILRLITRTFDLEK 528 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,410,082 Number of extensions: 105727 Number of successful extensions: 305 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 2 Length of query: 709 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 617 Effective length of database: 1,184,250 Effective search space: 730682250 Effective search space used: 730682250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)