BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3721.p (266 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 502 e-143 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 128 6e-31 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 124 7e-30 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 118 6e-28 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 117 8e-28 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 502 bits (1293), Expect = e-143, Method: Compositional matrix adjust. Identities = 241/266 (90%), Positives = 258/266 (96%) Query: 1 MDKLMQDRAIIVTGAGSVGEGWGNGKAAAVLYARQGGRVLAVDRSIQAALETQDIIRREG 60 MDKLMQ +AIIVTGAGSVGEGWGNGKAAAVLYAR+GGRVLAVDRSI+AAL+TQDIIR EG Sbjct: 1 MDKLMQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQDIIRSEG 60 Query: 61 GICEVLAVDVSRSDEVKAMAEAAMDYFGRVDVLHNNVGIAELGGAVETSEEDWNRLVAVN 120 G+CEVLA DVSR+ EVKA+AEAAMD+FGRVDVLHNNVGIA++GG VETSEE WNRLVAVN Sbjct: 61 GVCEVLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVN 120 Query: 121 QTSLFLTHKHVLPIMERQRKGAIVNISSVAALRWIGFPYLGYSATKAAILAFTKNVAMQY 180 QTSLFLTHKHVLPIME+QRKGAIVNISS+AALRWIGFPYLGY+ATKAAILAFTKNVAMQY Sbjct: 121 QTSLFLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQY 180 Query: 181 AAMGIRANCVLPGMMNTPMIREPLKAAYGGDIEQMCAQRDAQCPMGFMGDAWDVAHASLF 240 A MGIRANCVLPGMM+TPMIREPLKA+YGGDIEQM A+R+AQCPMGFMGDAWDVAHASLF Sbjct: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 Query: 241 LASDHARYITGLDMIVDGGLSLRCAG 266 LASDHARYITGLDMIVDGGLSLRCAG Sbjct: 241 LASDHARYITGLDMIVDGGLSLRCAG 266 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 128 bits (321), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 90/261 (34%), Positives = 144/261 (55%), Gaps = 20/261 (7%) Query: 4 LMQDR-AIIVTGAGSVGEGWGNGKAAAVLYARQGGRVLAVDRSIQAALETQDIIRREGGI 62 L++D+ AII GAG G G+ + A L A QG R++ +D ++ A E R G Sbjct: 2 LLKDKVAIITGGAGQNGLGF----STARLMAAQGARIVILD--LERA-EPAGAAARLGAQ 54 Query: 63 CEVLAVDVSRSDEVKAMAEAAMDYFGRVDVLHNNVGIAELGGAVETSEEDWNRLVAVNQT 122 L DV+ +A A A + FGR+D+L NN G+ + +E + +D++R++ V+ Sbjct: 55 HLGLVADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLR 114 Query: 123 SLFLTHKHVLPIMERQRKGAIVNISSVAALR---WIGFPYLGYSATKAAILAFTKNVAMQ 179 + VLP M+ Q+ G+IV ISSV+A R +G P+ YSA KA +L + +A + Sbjct: 115 GTLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMARE 172 Query: 180 YAAMGIRANCVLPGMMNTPMIREPLKAAYGGDIEQMCAQRDAQCPMGFMGDAWDVAHASL 239 + GIR NC+ PG+++T + ++ L A +I Q P+G +G DV + Sbjct: 173 FGPQGIRVNCITPGLISTDINKDILSAEKKAEIAQT-------IPLGRLGGPDDVGGVCV 225 Query: 240 FLASDHARYITGLDMIVDGGL 260 FLASD ++Y TG+ + V+GG+ Sbjct: 226 FLASDLSQYCTGVTLDVNGGM 246 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 124 bits (312), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 83/260 (31%), Positives = 134/260 (51%), Gaps = 18/260 (6%) Query: 4 LMQDRAIIVTGAGSVGEGWGNGKAAAVLYARQGGRVLAVDRSIQAALETQDIIRREGGIC 63 L++D+ ++TG + G G A A L A QG RV+ +D + R G Sbjct: 2 LLKDKVAVITGGAGIN---GLGYATARLMAEQGARVVVLDLAQADPAAAA---ARIGSQH 55 Query: 64 EVLAVDVSRSDEVKAMAEAAMDYFGRVDVLHNNVGIAELGGAVETSEEDWNRLVAVNQTS 123 L DV+ + A A + FGR+D+L NN GI + +E + D++R++ V+ Sbjct: 56 LGLVADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRG 115 Query: 124 LFLTHKHVLPIMERQRKGAIVNISSVAALR---WIGFPYLGYSATKAAILAFTKNVAMQY 180 + VLP M Q+ G+I+ ISSV+A R +G P+ YSA KA +L + +A ++ Sbjct: 116 TLYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREF 173 Query: 181 AAMGIRANCVLPGMMNTPMIREPLKAAYGGDIEQMCAQRDAQCPMGFMGDAWDVAHASLF 240 GIR N + PG++ T +I+ L E+ A+ P+ +G A D+ A +F Sbjct: 174 GPEGIRVNSITPGLIGTDIIKGKLT-------EEKKAEIAETIPLARLGRADDIGGACVF 226 Query: 241 LASDHARYITGLDMIVDGGL 260 L SD ++Y TG+ + V+GG+ Sbjct: 227 LGSDLSQYCTGITLDVNGGM 246 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 118 bits (295), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 80/256 (31%), Positives = 132/256 (51%), Gaps = 15/256 (5%) Query: 5 MQDRAIIVTGAGSVGEGWGNGKAAAVLYARQGGRVLAVDRSIQAALETQDIIRREGGICE 64 + + +IVTGA S G G+A A ++ +G RVL DR A + D + E Sbjct: 12 FEGKVVIVTGAAS-----GIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAAR 66 Query: 65 VLAVDVSRSDEVKAMAEAAMDYFGRVDVLHNNVGIAELGGAVETSEEDWNRLVAVNQTSL 124 DVS ++V+ + + A++ FG++DVL ++ G+ G E S EDW+R+ A N + Sbjct: 67 --ETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGV 124 Query: 125 FLTHKHVLPIMERQRKGAIVNISSVAALRWIGFPYLGYSATKAAILAFTKNVAMQYAAMG 184 F + LP +E+ R G IVN++SV+ L + Y+A+K A+ T+ +A+ + G Sbjct: 125 FYGAREALPHLEKTR-GCIVNVASVSGL-AADWNLSAYNASKGAVCNLTRAMALDFGRKG 182 Query: 185 IRANCVLPGMMNTPMIREPLKAAYGGDIEQMCAQRDAQCPMGFMGDAWDVAHASLFLASD 244 +R N V P + +T M A D + + + +G D ++A FLAS Sbjct: 183 VRINAVCPSLTHTAMT------ADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASP 236 Query: 245 HARYITGLDMIVDGGL 260 A ++ G+++ VDGGL Sbjct: 237 DASFVNGVNLPVDGGL 252 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 117 bits (294), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 83/257 (32%), Positives = 134/257 (52%), Gaps = 17/257 (6%) Query: 8 RAIIVTGAGSVGEGWGNGKAAAVLYARQGGRVLAVDRSIQAALETQDIIRREGGICEVLA 67 + +++T A G G+A+ L+AR+G RV+A D S E I G+ E Sbjct: 11 KTVLITAAAQ-----GIGRASTELFAREGARVIATDISKPHLDELAGI----AGV-ETHL 60 Query: 68 VDVSRSDEVKAMAEAAMDYFGRVDVLHNNVGIAELGGAVETSEEDWNRLVAVNQTSLFLT 127 +DV+ +KA+ + G +DVL N G G +E ++ W+ +N ++F T Sbjct: 61 LDVTDDAAIKAL----VAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHT 116 Query: 128 HKHVLPIMERQRKGAIVNISSVAALRWIGFPYLGYSATKAAILAFTKNVAMQYAAMGIRA 187 + VLP M ++ G+IVNI+S A+ Y A+KAA++ TK+VA + A GIR Sbjct: 117 IRAVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRC 176 Query: 188 NCVLPGMMNTPMIREPLKA---AYGGDIEQMCAQRDAQCPMGFMGDAWDVAHASLFLASD 244 N + PG + +P + + + G E++ A + PMG +G A +VA +L+LASD Sbjct: 177 NAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLASD 236 Query: 245 HARYITGLDMIVDGGLS 261 + + TG ++DGG S Sbjct: 237 ESNFTTGSIHMIDGGWS 253 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 844,700 Number of extensions: 34732 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 45 Length of query: 266 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 182 Effective length of database: 1,222,130 Effective search space: 222427660 Effective search space used: 222427660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)