BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.3694.p
         (336 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313077|ref|YP_003777169.1| coenzyme F420-dependent N5,N10-...   639   0.0  
gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent N5,N10-...    65   1e-11

>gi|300313077|ref|YP_003777169.1| coenzyme F420-dependent
           N5,N10-methylene tetrahydromethanopterin reductase
           [Herbaspirillum seropedicae SmR1]
          Length = 336

 Score =  639 bits (1648), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 310/336 (92%), Positives = 324/336 (96%)

Query: 1   MIPFSVLDLSPINQGSSAAQAFQNSKELAQRAEALGYRRYWLAEHHNMPGIASAATALVI 60
           MIPFSVLDLSPINQGS+AAQAF NSKELAQRAEALGY RYWLAEHHNMPGIASAATALVI
Sbjct: 1   MIPFSVLDLSPINQGSTAAQAFNNSKELAQRAEALGYHRYWLAEHHNMPGIASAATALVI 60

Query: 61  SHIAAHTRTIRVGSGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGRAPGSDQRTARAL 120
           SH+AA+TR+IRVGSGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGRAPGSDQRTARAL
Sbjct: 61  SHVAANTRSIRVGSGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGRAPGSDQRTARAL 120

Query: 121 RRHMGGDTADNFPQDVLELQQYFDEPSDYQALRAVPGAGLKVPLWLLGSSMFSAQLAAEL 180
           RRHMGGDTAD FPQDV ELQ YFD+P+DYQ LRAVPGAGLKVP+WLLGSSMFSAQLAAEL
Sbjct: 121 RRHMGGDTADTFPQDVQELQDYFDDPADYQTLRAVPGAGLKVPIWLLGSSMFSAQLAAEL 180

Query: 181 GLPFAFASHFAPGFMKSAVQIYRERFKPSANLKKPYVMLALNVFAADTQRQAERLFTSLQ 240
           GLPFAFASHFAPGFMKSAVQIYRERFKPSA LKKPYVMLALNVFAADTQR+AERLFTSLQ
Sbjct: 181 GLPFAFASHFAPGFMKSAVQIYRERFKPSATLKKPYVMLALNVFAADTQREAERLFTSLQ 240

Query: 241 QQFLNLIRGTPGQLRPPVEDIDALWQPGEQAHVERSLACAVVGDRETVQEGLQAFIDEHA 300
           QQF+NLIRGTPGQLRPPV+DIDALWQPGEQAHV RSLACAVVGDR+ VQ+GLQAF+DEHA
Sbjct: 241 QQFMNLIRGTPGQLRPPVDDIDALWQPGEQAHVARSLACAVVGDRQAVQDGLQAFMDEHA 300

Query: 301 PDELIITAQIFDHAARLHSFEIAAEARAALQERVAI 336
           PDELIITAQIFDHAARLHSFEIAAEAR AL+E V I
Sbjct: 301 PDELIITAQIFDHAARLHSFEIAAEARQALREPVTI 336


>gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent
           N5,N10-methylene tetrahydromethanopterin reductase
           [Herbaspirillum seropedicae SmR1]
          Length = 333

 Score = 64.7 bits (156), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 43/120 (35%), Positives = 62/120 (51%), Gaps = 7/120 (5%)

Query: 13  NQGSSAAQAFQNSKELAQRAEALGYRRYWLAEHHNMPGIASAATALVISHIAAHTRTIRV 72
           N   +A  A   + + A  AEALGY   W+AEHH       +A  ++++ IAA T  IR+
Sbjct: 16  NPHGNARDALLQTVDHAVVAEALGYHDVWVAEHHYSSFAIGSALMVLLAQIAARTSRIRL 75

Query: 73  GSGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGR-APGSDQRTARALRRHMGGDTADN 131
           G+G  +L  + PL +AE   TL+ L  GR++ G+ R  P  +Q       RH G   AD 
Sbjct: 76  GTGASLLALNDPLRVAEDVATLDLLSNGRMEFGVARGGPFPEQ------YRHAGLSGADQ 129


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.134    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,103,375
Number of extensions: 47596
Number of successful extensions: 145
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 145
Number of HSP's successfully gapped: 2
Length of query: 336
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 250
Effective length of database: 1,212,660
Effective search space: 303165000
Effective search space used: 303165000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 131 (55.1 bits)