BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3693.p (527 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313075|ref|YP_003777167.1| gamma-glutamyltranspeptidase [H... 907 0.0 gi|300310422|ref|YP_003774514.1| gamma-glutamyltranspeptidase [H... 179 4e-46 >gi|300313075|ref|YP_003777167.1| gamma-glutamyltranspeptidase [Herbaspirillum seropedicae SmR1] Length = 527 Score = 907 bits (2344), Expect = 0.0, Method: Compositional matrix adjust. Identities = 467/527 (88%), Positives = 499/527 (94%) Query: 1 MLYSKTCLGGMVTAPHHLAAQAGAQILREGGNAVEAMVAAAATIAVVYPHMNGIGGDGFW 60 MLYSKTCLGGMV+APHHLAAQAGAQILR+GGNAVEAMVAAAATIAVVYPHMNGIGGDGFW Sbjct: 1 MLYSKTCLGGMVSAPHHLAAQAGAQILRQGGNAVEAMVAAAATIAVVYPHMNGIGGDGFW 60 Query: 61 VISEAGQEPVAIRACGPAAALATQDFYAGHGHRTMPTRGSHAALTVAGAVAGWSAALDYA 120 +ISE G+ PVAIRACGPAAALA + FYA G+R MPTRG HAALTVAGAVAGW+AAL++A Sbjct: 61 LISEPGRAPVAIRACGPAAALANKAFYAERGYRAMPTRGPHAALTVAGAVAGWAAALEHA 120 Query: 121 QRFGRALPLPMLLADAIRHAQTGVPITRSQHELTRAKWDELKDQPGFAATYAADGIPSQG 180 QRF LPLP+LLADAI HAQ G+P+TRSQHELTR KW ELKDQPGFAATYAADG+P QG Sbjct: 121 QRFASGLPLPVLLADAIGHAQAGIPVTRSQHELTRDKWSELKDQPGFAATYAADGVPPQG 180 Query: 181 KVQKQSALAASLSRLAEAGLDDFYRGDLAQRLAAGLQAVGSPLRRSDLEAFRAQLLPPLM 240 KV KQSALAASLSRLAEAGLDDFYRGDLAQ LAAGLQA GSPLRRSDLEAFRAQ++ PLM Sbjct: 181 KVLKQSALAASLSRLAEAGLDDFYRGDLAQSLAAGLQAAGSPLRRSDLEAFRAQVVQPLM 240 Query: 241 VQLRSGRVYNLPPPTQGVSALMIIGLFDRLGVPQAEGFAHIHGLVEATKRAFILRNQHVT 300 VQL SGRVYNLPPPTQGVSALMIIGLFDRLGV QA+GFAH+HGLVEATKRAFILRNQHVT Sbjct: 241 VQLSSGRVYNLPPPTQGVSALMIIGLFDRLGVTQADGFAHLHGLVEATKRAFILRNQHVT 300 Query: 301 DPAHMSVEAAHWLLAEALDQEARQIDMQRAAPWPHPAKPGDTIWMGAADAQGRVVSYIQS 360 DPAHM V+AA WLLA ALDQE R IDMQRAAPWPHPA+PGDTIWMGAADAQGRVVSYIQS Sbjct: 301 DPAHMRVDAAQWLLAGALDQEVRHIDMQRAAPWPHPARPGDTIWMGAADAQGRVVSYIQS 360 Query: 361 IFWEFGSGVVVPDTGVVWQNRGASFTLDDGVNQLQPGKLPFHTLNPSLARLNDGRTLAYG 420 IFWEFGSGVVVPDTG+VWQNRGASFTLD+GVNQLQPGKLPFHTLNPSLA+L+DGRTLA+G Sbjct: 361 IFWEFGSGVVVPDTGIVWQNRGASFTLDEGVNQLQPGKLPFHTLNPSLAQLDDGRTLAFG 420 Query: 421 TMGGEGQPQTQAAVFTRHVLFGQNLQAAISAPRWLLGRTWGDVSTNLKVEGRVPDATIEA 480 TMGGEGQPQTQAAVFTRHVLFGQNLQAA+SAPRWLLGRTWGDVSTNLK+EGR+P+ATI+A Sbjct: 421 TMGGEGQPQTQAAVFTRHVLFGQNLQAAVSAPRWLLGRTWGDVSTNLKLEGRIPEATIQA 480 Query: 481 LRRAGHEVQVVEDYTDMMGHAGAVCVHPDGVIEGATDPRADGVCAGV 527 LRRAGHEVQVV+DY DMMGHAGAVCVHPDG+IEGATDPRADGVCAG+ Sbjct: 481 LRRAGHEVQVVDDYADMMGHAGAVCVHPDGLIEGATDPRADGVCAGL 527 >gi|300310422|ref|YP_003774514.1| gamma-glutamyltranspeptidase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 179 bits (455), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 155/544 (28%), Positives = 248/544 (45%), Gaps = 41/544 (7%) Query: 10 GMVTAPHHLAAQAGAQILREGGNAVEAMVAAAATIAVVYPHMNGIGGDGFWVISEAGQ-E 68 GMV LA + G IL++GGNAV+A VA +AVV P+ IGG GF +I +A + Sbjct: 55 GMVATEQELATKVGVDILKKGGNAVDAGVAVGFALAVVLPNAGNIGGGGFMMIHDAKTGK 114 Query: 69 PVAIRACGPAAALATQDFYAGHGHRTMPTRG--SHAALTVAGAVAGWSAALDYAQRFGRA 126 VA+ A AT++ Y +P R +H A+ V G VAG S AL ++G Sbjct: 115 NVALDFREMAPQRATRNMYLDDKGNVVPGRSLYTHLAVGVPGTVAGLSHAL---SKYG-T 170 Query: 127 LPLPMLLADAIRHAQTGVPITRSQHELTRAKWDELKDQPGFAATYAADGIPSQ-GKVQKQ 185 + L ++ A+ A+ G P++RS ++ A+ + L A + +G P Q G++ Q Sbjct: 171 MKLSQVIQPAVELAEKGYPVSRSLAQILSAEREHLGQWDSSKAIFFKEGRPLQEGELLVQ 230 Query: 186 SALAASLSRLAEAGLDDFYRGDLAQRLAAGLQAVGSPLRRSDLEAFRAQLLPPLMVQLRS 245 LA SL +A+ G FY G +A+++ A + G + DL+ ++ P+ R Sbjct: 231 KDLAKSLRLIAKEGPKAFYEGSIAKKIVAEMDKHGGLISADDLKNYKVVERVPVSGNYRG 290 Query: 246 GRVYNLPPPTQ-GVSALMIIGLFDRLGVPQ--AEGFAHIHGLVEATKRAFILRNQHVTDP 302 +V ++PPP+ GV + ++ + +R + Q A+ IH + E K A+ R++++ DP Sbjct: 291 YQVMSMPPPSSGGVHIIQMMNILERYPLKQYGADSAQTIHLMAETMKLAYADRSEYLGDP 350 Query: 303 AHMSVEAAHWLLAEALDQEARQIDMQRAAP-------WPHPAKPGDTIWMGAADAQGRVV 355 V D+ A++I+ RA P P P + T AD G +V Sbjct: 351 DFTKVPVKGLTSRAYADELAKKINPDRATPSSEIKPGQPQPYESDQTTHYSVADKDGNLV 410 Query: 356 SYIQSIFWEFGSGVVVPDTGVVWQNRGASFTLDDGV-----------NQLQPGKLPFHTL 404 + ++ FGSG+V TG+ N F GV N + P K P ++ Sbjct: 411 ATTYTLNLNFGSGIVATGTGITLNNEMDDFAAKPGVPNAFGLVGGDANAVAPYKRPLSSM 470 Query: 405 NPSLARLNDGRT-LAYGTMGGEGQPQTQAAVFTRHVLFGQNLQAAISAPRWLLGRTWGDV 463 +P+ L DG+ L G+ GG T + N+ A PR + W Sbjct: 471 SPTFV-LKDGKPFLVTGSPGGSRIITTTLQTILNVIDHDMNVAEATITPR--IHHQWA-- 525 Query: 464 STNLKVEGRVPDATIEALRRAGHEVQVVEDYTDMMGHAGAVCVHPDGVIEGATDPRA-DG 522 L+VE + T++ L+ G ++ V MG + + DG +G +DPR DG Sbjct: 526 PDQLRVEKGLSADTLKILQDKGQKISVQPS----MGRTQTIQIRSDG-FDGFSDPRNPDG 580 Query: 523 VCAG 526 G Sbjct: 581 RTLG 584 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,829,777 Number of extensions: 82719 Number of successful extensions: 289 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 284 Number of HSP's successfully gapped: 2 Length of query: 527 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 437 Effective length of database: 1,193,720 Effective search space: 521655640 Effective search space used: 521655640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)