BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3690.p (732 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313072|ref|YP_003777164.1| high-affinity K+ transport syst... 1298 0.0 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 141 2e-34 gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbas... 135 1e-32 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 125 2e-29 gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase ... 107 3e-24 >gi|300313072|ref|YP_003777164.1| high-affinity K+ transport system ATPase [Herbaspirillum seropedicae SmR1] Length = 730 Score = 1298 bits (3359), Expect = 0.0, Method: Compositional matrix adjust. Identities = 683/732 (93%), Positives = 702/732 (95%), Gaps = 2/732 (0%) Query: 1 MKNSSSNTPAGSTQAVPADAIPSAAQTARWMFDPKIVKPALADSFRKLSPRVQWRNPVMF 60 M SSNT A +T V +AIPSAAQTARWMFDPKIVKPALADSFRKLSPR QWRNPVMF Sbjct: 1 MTTPSSNTSASAT--VTPEAIPSAAQTARWMFDPKIVKPALADSFRKLSPRAQWRNPVMF 58 Query: 61 VVYLGSILTTLLFIQALVGKGEAPAGFIFAIAAWLWFTVLFANFAEALAEGRSKAQAAAL 120 VVYLGSILTTLLFIQA VG+GEAPAGFIFAIAAWLWFTVLFANFAEALAEGRSKAQAAAL Sbjct: 59 VVYLGSILTTLLFIQAAVGQGEAPAGFIFAIAAWLWFTVLFANFAEALAEGRSKAQAAAL 118 Query: 121 RGVKKSVQAKKLRSADYTRGMDAANAARQCEVVEGGSLRKGDLVFIEAGDTVPADGDVIE 180 RGVKKSV AKKLR AD+ RG A QCEVVEG SLRKGDLV IEAGDTVPAD +VIE Sbjct: 119 RGVKKSVMAKKLRRADHVRGDGMQVLASQCEVVEGDSLRKGDLVLIEAGDTVPADAEVIE 178 Query: 181 GVATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVVRVSVNPGEAFLDRMIAMVE 240 GVATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVVRVSVNPGEAFLDRMIAMVE Sbjct: 179 GVATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVVRVSVNPGEAFLDRMIAMVE 238 Query: 241 GARRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVTAAGTGSPVTVTALIALLVCL 300 GARRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSV AAG+GSPV+VTALIALLVCL Sbjct: 239 GARRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVNAAGSGSPVSVTALIALLVCL 298 Query: 301 IPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAF 360 IPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAF Sbjct: 299 IPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAF 358 Query: 361 MPAPGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMAGLGAEFIPFSAQTR 420 MPAPGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMA LGAEFIPFSAQTR Sbjct: 359 MPAPGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMANLGAEFIPFSAQTR 418 Query: 421 VSGIDIGHRRIRKGAADAIRKLVRSADQRLPAQVESDVEAIARRGGTPLVVIEWNNEAAV 480 VSGID+GHRRIRKGAADAIRKL R+ADQRLPAQVESD+E+IARRGGTPLVVIEW+ EAA+ Sbjct: 419 VSGIDLGHRRIRKGAADAIRKLTRAADQRLPAQVESDIESIARRGGTPLVVIEWHAEAAM 478 Query: 481 IHQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTIMITGDNRLTAAAIAAEAGVDDF 540 I+QGAPIRILGTVELKDIVKGGIKERFGELRRMGIKT+MITGDNRLTAAAIAAEAGVDDF Sbjct: 479 INQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTVMITGDNRLTAAAIAAEAGVDDF 538 Query: 541 LAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVD 600 LAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVD Sbjct: 539 LAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVD 598 Query: 601 LDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFVANYPQLGALNVMRL 660 LDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFV +YPQLGALNVM L Sbjct: 599 LDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFVGSYPQLGALNVMHL 658 Query: 661 ASPESAIMSAVIFNALVIVALIPLALRGVRYRALGAAVLLRRNLLIYGVGGLLVPFVGIK 720 ASP+SAIMSAVIFNALVIVALIPLALRGVRYRALGAAVLLRRNLLIYGVGGLLVPFVGIK Sbjct: 659 ASPDSAIMSAVIFNALVIVALIPLALRGVRYRALGAAVLLRRNLLIYGVGGLLVPFVGIK 718 Query: 721 LIDMLLAAFGLV 732 LID+LL+AFGLV Sbjct: 719 LIDLLLSAFGLV 730 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 141 bits (356), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 153/540 (28%), Positives = 251/540 (46%), Gaps = 58/540 (10%) Query: 157 SLRKGDLVFIEAGDTVPADGDVIEGVATVDESAITGESAPVIRESGGDFSAVTGGTRVLS 216 +R GDLV + G+ VP DG V EG + +DE+ +TGES P +++G +T G+ Sbjct: 246 QVRVGDLVVLRPGERVPVDGVVREGRSHLDEAMLTGESRPQAKQAG---DPLTAGSINAE 302 Query: 217 DWIVVRVSVNPGEAFLDRMIAMVEGARRQKTPNELALTLLLVALTLVFL-LVTVTLLPFS 275 +V+ + E L R++ MVE A+ K P + L+ ++ VF+ V + L Sbjct: 303 GVLVMETTAIGAETVLARIVRMVEQAQAAKAP----IQRLVDQVSAVFVPAVLLLSLLTL 358 Query: 276 IFSVTAAGTGSPVTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEA 335 + G + A +A++V P +G +AG + ++ A+E Sbjct: 359 LGWGLVQGNWEQALLNA-VAVMVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALEI 417 Query: 336 AGDVDVLLLDKTGTITLGNRQAAAFMPAPGVTERELADVAQLASLADETPEGRSIVVLAK 395 A V+ + DKTGT+TLG+ + P EL +A E P GR++ A Sbjct: 418 AHRVNAVAFDKTGTLTLGHPRLLELEATPSTQPDELLALAAALQAHSEHPLGRAVREAAA 477 Query: 396 NRFNLRE-----REMAGLGAEFIPFSAQTRVSGIDIGHRRIRKGAADAIRKLVRSADQRL 450 R +AGLG E + V G R++ G++ +++L A Sbjct: 478 QSAQAARQAEQVRSVAGLGIEGV-------VEG-----RQLMLGSSRWMQQLQAHA---- 521 Query: 451 PAQVESDVEAIARRGGTPLVVIEW---NNEAAVIHQGAPIRILGTVELKDIVKGGIKERF 507 PA +++ E + +G T + W N++ V +LG + D +K G + Sbjct: 522 PA-LQARAEQLQAQGRT----LSWLARRNDSGV-------EVLGLLAFGDQIKPGAQAAI 569 Query: 508 GELRRMGIKTIMITGDNRLTAAAIAAEAGVDDFLAEATPEQKL-ALIRQHQAEGKLVAMT 566 L RMG+++ M++GDN A +A+E G+ + AE P K A+ Q ++VAM Sbjct: 570 ATLHRMGVRSAMLSGDNLGAAQQVASELGIQEVRAEVLPADKAEAVTALRQGGAQVVAMV 629 Query: 567 GDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLT 626 GDG NDAPALA ADV +AM++GT A +A + + +P + + ++I ++ T Sbjct: 630 GDGVNDAPALAAADVGMAMSTGTDVAMQAAGVTLMHGDPGLVADAIDISRR--------T 681 Query: 627 TFSIANDVAKYFAIIPAMFVANYPQLGALNVMRLASPESAIMS-AVIFNALVIVALIPLA 685 I ++ ++A I + LG LN M LA A S +V+ NAL++ P A Sbjct: 682 YAKIRQNL--FWAFIYNLVGIPLAALGLLNPM-LAGAAMAFSSVSVVSNALLLRRWRPHA 738 >gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbaspirillum seropedicae SmR1] Length = 853 Score = 135 bits (340), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 165/605 (27%), Positives = 263/605 (43%), Gaps = 95/605 (15%) Query: 111 GRSKAQAAALRGVKKSVQAKKLRSADYTRGMDAANAARQCEVVEGGSLRKGDLVFIEAGD 170 G+++ AALR V S A+ +R R + L +GDL+ +E G Sbjct: 87 GKTERALAALR-VLNSPTARVIRDGRTMR-------------IAAAELVRGDLILLEEGA 132 Query: 171 TVPADGDVIEGVA-TVDESAITGESAPVIR------------ESGGDFSA--VTGGTRVL 215 VPAD V+E +DES +TGE+ PV + SG SA V GT V+ Sbjct: 133 RVPADAGVLEASNLQLDESLLTGEACPVEKFAPSTKPEPHALPSGQTDSATLVRSGTLVV 192 Query: 216 SDWIVVRVSVNPGEAFLDRMIAMVEGARRQKTPNELALTLLLVALTLVFLLVTVTLLPFS 275 + +V+ L ++ +++ + +P + + ++ L + L+++ L+ Sbjct: 193 RGSGIAKVTATGARTELGKISSLLHELVEEPSPMQRHIARIVTVLAVTGLMLSALLVVLE 252 Query: 276 IFSVTAAGTGSPVTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEA 335 +S G + + IAL + L+P + +L+ G RM +ANV+ A+E Sbjct: 253 GYS---HGRWTEALLMG-IALSMSLLPEELPIILTIFPAIGAWRMARANVLTRRLSAIET 308 Query: 336 AGDVDVLLLDKTGTITLGNRQAAAFMPAP----------GVTE---RELADVAQLASLAD 382 G + VL DKTGT+T + M A G+T R L + A LAS Sbjct: 309 LGSISVLCTDKTGTLTENRMRVVQGMIAGRHMYFDESDIGITSPEWRTLLETAALASSPR 368 Query: 383 ET-PEGRSIVVLA---KNRFNLREREMAGLGAEFIPFSAQTRV-----SGIDIGHRRI-R 432 T P ++ LA RF + L E+ PF+ R G D + Sbjct: 369 PTDPMEKAFHQLAGESAKRFA--PPALTQLLQEY-PFTPALRAMTMVWKGDDDAPLLVAT 425 Query: 433 KGAADAIRKLVRSADQRLPAQVESDVEAIARRGGTPLVVI-----EWNNEAAVIHQGAPI 487 KGA +AI L PA+V+ I G L VI W+ + Sbjct: 426 KGAPEAIASLCGLT----PAEVQQMQNDIMHLGSHGLRVIAVAACNWSGALPASQAQFGL 481 Query: 488 RILGTVELKDIVKGGIKERFGELRRMGIKTIMITGDNRLTAAAIAAEAGVD--------- 538 R LG + L D ++ G+ + + I+ IMITGD+ TA IA +AG+ Sbjct: 482 RYLGLLALADPLRPGVPDAIQRCHQAEIRVIMITGDHPSTAQNIARQAGISADQVLLGDE 541 Query: 539 ----------------DFLAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVA 582 + A TP QKL ++ + +G+++AMTGDG NDAPAL A V Sbjct: 542 VQAMAEHELARRLRTTNVCARITPLQKLRIVAALKDDGEIIAMTGDGVNDAPALKAAHVG 601 Query: 583 VAMNS-GTQAAKEAGNMVDLDSNPTKLIEIVEIGKQML-MTRGSLTTFSIANDVAKYFAI 640 +AM + GT A+EAG+++ LD N ++E + +G+++ RG++ + A+ Sbjct: 602 IAMGARGTDVAREAGSLILLDDNFASIVEGILMGRRIFSRMRGAMIYVLAMHMPIAGMAL 661 Query: 641 IPAMF 645 +P +F Sbjct: 662 LPVVF 666 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 125 bits (313), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 135/471 (28%), Positives = 220/471 (46%), Gaps = 59/471 (12%) Query: 162 DLVFIEAGDTVPADGDVIEGVATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVV 221 D V + G+ VP DG V+ G +++++ ITGES PV +++G V GT + Sbjct: 290 DTVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQAG---DPVFAGTINEQGAFEM 346 Query: 222 RVSVNPGEAFLDRMIAMVEGARRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVTA 281 RV+ G++ L R++ V+ A+ Q+ P + + A + +V + +L + + A Sbjct: 347 RVTAAQGDSTLARIVKSVQQAQGQRAPTQRFVDNF--ARYYIPAVVVMAVLVALLPPLLA 404 Query: 282 AGTGSPVTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDV 341 P AL+ LLV P + V+G++ + ++ G +E + Sbjct: 405 GAAFQPWLYKALV-LLVIACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQLRA 463 Query: 342 LLLDKTGTITLGNRQAAAFMPAPGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLR 401 L LDKTGT+T G P VT DV L + AD+ + V LA + Sbjct: 464 LALDKTGTLTFGK---------PRVT-----DVVSLQAQADQQALLQLAVSLAARSDHPV 509 Query: 402 EREMAG----LGAEFIPFSA------QTRVSG--IDIGHRRIRKGAADAIRKLVRSADQR 449 R +A E F+A Q R++G + +G+ R LV Q Sbjct: 510 SRAIAAQEEIATVEVSEFAALAGRGVQGRINGQLLHLGNHR-----------LVHELGQC 558 Query: 450 LPAQVESDVEAIARRGGTPLVVIEWNNEAAVIHQGAPIRILGTVELKDIVKGGIKERFGE 509 P ++E+ ++A ++G T V+ G P + V + D V+ ++ E Sbjct: 559 SP-ELEARLQAFEKQGKTTTVLC---------LDGQPQLL---VAVADTVRPSSRQAVAE 605 Query: 510 LRRMGIKTIMITGDNRLTAAAIAAEAGVDDFLAEATPEQKLALIRQ-HQAEG-KLVAMTG 567 LR +G++ IM+TGDN TA AIAA+ G+ D + PE KL I + Q G + V M G Sbjct: 606 LRALGVEVIMLTGDNSHTAQAIAAQTGISDARGDQLPEDKLKAIEELSQRHGVRGVGMVG 665 Query: 568 DGTNDAPALAQADVAVAMN-SGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQ 617 DG NDAPALA+AD+ AM +GT A E ++ +D + K+ + + + ++ Sbjct: 666 DGINDAPALARADIGFAMGAAGTDTALETADVALMDDDLRKIPDFIRLSRK 716 >gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 839 Score = 107 bits (268), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 131/520 (25%), Positives = 227/520 (43%), Gaps = 51/520 (9%) Query: 148 RQCEVVEGGSLRKGDLVFIEAGDTVPADGDVIEGVATVDESAITGESAPVIRESGGDFSA 207 R+ +V LR+GD++ GDT+ AD ++EG D + ++GES P + G + Sbjct: 332 RETTLVVAAQLREGDVILARPGDTIAADACLLEGAGEFDLALLSGESRPQSFQIGQE--- 388 Query: 208 VTGGTRVLSDWIVVRVSVNPGEAFLDRMIAMVEGARRQKTP----NELALTLLLVALTLV 263 GG LS + +RV GE+ L + + E A + K ++ + +VAL Sbjct: 389 APGGAVNLSQPVFLRVVRATGESTLAALTRLAEQAGQGKPALAQWADVVASRFVVAL--- 445 Query: 264 FLLVTVTLLPFSIFSVTAAGTGSPVTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQA 323 LL T L + + T A + + IA+LV P + + A R+++A Sbjct: 446 LLLAGATFLAWQLIDPTRAWSTA-------IAVLVVSCPCALSLATPSALAAATDRLLRA 498 Query: 324 NVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAFMPAPGVTERELADVAQLASLADE 383 + G +E D ++ DKTGT+T G Q A +DV SLA Sbjct: 499 GTLIVRGNVLETLQRADTIVFDKTGTLTQGRPQLTALW----------SDVDCQRSLA-- 546 Query: 384 TPEGRSIVVLAKNRFNLREREMAGLGAEFIPFSAQTRVSGIDIGHRRIRKGAADAIRKLV 443 + A R +L L + A+ +V +++ GA ++ ++ Sbjct: 547 -------IAAAMERGSLHP-----LAKALVEEGARRQVDALEVEQLTSVTGAG--MQGMI 592 Query: 444 RSADQRLPAQVESDVEAIARRGGTPLVVIEWNNEAAVIHQGAPIRILGTVELKDIVKGGI 503 R+ ++ S V +A P+++ + A ++ G+ L +L D ++ Sbjct: 593 EGDLYRIGSR--SFVAGLAGTALPPVLLQSAASGAGSVYLGSSDGWLARFDLADALRTDA 650 Query: 504 KERFGELRRMGIKTIMITGDNRLTAAAIAAEAGVDDFLAEATPEQKLALIRQHQAEGKLV 563 R +G++TI+++GD A +AA AG+ + A TP+ KL +R Q +G +V Sbjct: 651 VATVAAFRALGLRTILLSGDQPEVCAEVAAAAGISEVQAACTPQGKLDYVRNLQRDGAVV 710 Query: 564 AMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQ-MLMTR 622 A+ GDG NDA L DV+ AM G A+ + + V + + + + ++ M + R Sbjct: 711 AIVGDGINDAAMLRAGDVSFAMGKGAALAQVSADAVIMSDRLQAVADCAAMAQRTMHIVR 770 Query: 623 GSLTTFSIANDVAKYFAIIPAMFVANYPQLGALNVMRLAS 662 +L + N + AI A F P + A+ M L+S Sbjct: 771 QNLVWAATYN----FLAIPAAAFGLLDPWMSAVG-MSLSS 805 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,122,038 Number of extensions: 86638 Number of successful extensions: 470 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 447 Number of HSP's successfully gapped: 7 Length of query: 732 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 640 Effective length of database: 1,184,250 Effective search space: 757920000 Effective search space used: 757920000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)