BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3662.p (465 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 872 0.0 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 191 1e-49 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 184 2e-47 gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 166 5e-42 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 163 3e-41 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 872 bits (2252), Expect = 0.0, Method: Compositional matrix adjust. Identities = 425/465 (91%), Positives = 449/465 (96%) Query: 1 MQSSHTNDHTQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL 60 MQS+ +N H QQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL Sbjct: 1 MQSTQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL 60 Query: 61 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL 120 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL Sbjct: 61 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL 120 Query: 121 LVLLRLAQGFAVAGEISGASSMILEHSPFGRRGYYSSFALQGVQAGQILAAAVFLPLAHY 180 LV++RLAQGFAVAGEISGASSMILEHSPFGRRGY+SSF LQGVQAGQILAAAVFLPLAHY Sbjct: 121 LVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHY 180 Query: 181 MPTEQFNVWGWRIPFLLSFLVIIVCWIIRRKVDETPVFKAADQQELKRRSPVVEAFRDCT 240 MP EQFNVWGWRIPFLLSFLVII W+IRR VDETPVFKAAD++ELKRRSPVV+AFRDC+ Sbjct: 181 MPAEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCS 240 Query: 241 PNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGVGFAKDVYLWIPVVGNIVAVLVIPYVG 300 PNMLRVVCMALMNVIPVVATIFGAAYAVQPAYG+GFAKDVYLWIPVVGNIVAVLVIPYVG Sbjct: 241 PNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVG 300 Query: 301 KLSDRIGRRLPIVVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF 360 KLSDRIGRR PI+VGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF Sbjct: 301 KLSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF 360 Query: 361 FPELFPARARVSAMAIGQNLGTAITAMLPALFTAVAPPGASHIWLTVGSIAFGITIVAAL 420 +PELFPARARVSAMAI QNLGTAITAMLPALFTAVAPPG+S+IWLTVGSIAF ITIV+AL Sbjct: 361 YPELFPARARVSAMAIAQNLGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFAITIVSAL 420 Query: 421 AAMSARETHRIPLEKLGEADAQPLPKNEYERLRHQAELEGRLAKS 465 AA+SARET+RIPLEKL + +AQPLP+NEY+RLR +AE+ GR AKS Sbjct: 421 AALSARETYRIPLEKLDDPNAQPLPRNEYDRLRAEAEVAGRYAKS 465 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 191 bits (484), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 124/383 (32%), Positives = 201/383 (52%), Gaps = 20/383 (5%) Query: 9 HTQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYV 68 QQ ++A + IG+ LE++DF +Y+ A +I +LFFP G+ T+++ ++AT+GVG+ Sbjct: 15 SNQQRRRAIVATVIGNGLEWFDFTVYSFFA-VIIAKLFFPTGNDLTSLLLAVATFGVGFF 73 Query: 69 ARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVLLRLAQ 128 RP+G +LG D GRK L + LM T +GL PTY+ +GL+AP ++V+ RL Q Sbjct: 74 MRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQ 133 Query: 129 GFAVAGEISGASSMILEHSPFGRRGYYSSFALQGVQAGQILAAAVFLPLAHYMPTEQFNV 188 GF+ GE+ A++ + E++P R YYSS+ + +L AAV + + Sbjct: 134 GFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALAS 193 Query: 189 WGWRIPFLLSFLVIIVCWIIRRKVDETPVFKAADQQELKRRSPVVEAFRDCTPNMLRVVC 248 WGWR+PFLL ++ V + IR ++ETP F +++ K SP+ E R+ Sbjct: 194 WGWRLPFLLGIIIGPVGYYIRHHLEETPTFL---EEKDKTDSPLKEVIRNFPRETSASFS 250 Query: 249 MALMNVIPVVATIFGAAYAVQPAYGVGFAK---DVYLWIPVVGNIVAVLVIPYVGKLSDR 305 M ++ + +F P Y V K +VG + +++ P VG+L+DR Sbjct: 251 MVILWTVCTYVLLF-----YMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADR 305 Query: 306 IGRRLPIVVGS----LLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFF 361 IGRR P++ GS L+ FAY+ + + L F L++G + Y + F Sbjct: 306 IGRR-PLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQ---LVFGTLIAAYTGPILAAF 361 Query: 362 PELFPARARVSAMAIGQNLGTAI 384 ELFPA+ + +++ NL I Sbjct: 362 SELFPAKVLSTGLSVAYNLAVTI 384 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 184 bits (466), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 123/380 (32%), Positives = 199/380 (52%), Gaps = 22/380 (5%) Query: 12 QSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVARP 71 Q+++A S IG+ALE++D IY A +I Q FFP GD + +++ + AT+GV + RP Sbjct: 16 QARRAIISSSIGNALEWFDILIYGAFAVVIAKQ-FFPTGDDSVSLLLTFATFGVSFFMRP 74 Query: 72 IGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVLLRLAQGFA 131 +GA VLG D GRK L+ + LM T + +P+Y +GL APA + L ++ QGF+ Sbjct: 75 LGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFS 134 Query: 132 VAGEISGASSMILEHSPFGRRGYYSSF--ALQGVQAGQILAAAVFLPLAHYMPT-EQFNV 188 GE +++ ++EH+P RRG++SS+ A QG+ +L AA+F + + M T EQ Sbjct: 135 AGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGI---SLLLAALFGAVLNNMLTPEQLAS 190 Query: 189 WGWRIPFLLSFLVIIVCWIIRRKVDETPVFKAADQQELKRRSPVVEAFRDCTPNMLRVVC 248 WGWR+PF+ L+ IRR +DE P FK + + K +P+ + F +L Sbjct: 191 WGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSE---KTNAPLRDTFAHQKMRLLIGAG 247 Query: 249 MALMNVIPVVATIFGAAYAVQ----PAYGVGFAKDVYLWIPVVGNIVAVLVIPYVGKLSD 304 +M + V +++ YAV+ PA+ A V I +G+ P VG LSD Sbjct: 248 SVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGS-------PLVGALSD 300 Query: 305 RIGRRLPIVVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFFPEL 364 +IGR ++ S L + + ++ L ++ + GV+ Y A P+ ++ Sbjct: 301 KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADI 360 Query: 365 FPARARVSAMAIGQNLGTAI 384 FP R + M++ N+ I Sbjct: 361 FPVATRGTGMSLAYNIAVTI 380 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 166 bits (419), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 109/316 (34%), Positives = 171/316 (54%), Gaps = 15/316 (4%) Query: 10 TQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVA 69 T + KK + +G+ E+YDF++Y + A++I Q FF DP TA I +L + G++ Sbjct: 14 TPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQ-FFAGTDPNTAFIFALLAFAAGFIV 72 Query: 70 RPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVLLRLAQG 129 RP GA V G LGD GRK L + +MG ST VG+LP Y+ +G+ AP +L++LR+ QG Sbjct: 73 RPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILIILRILQG 132 Query: 130 FAVAGEISGASSMILEHSPFGRRGYYSSFALQGVQAGQILAAAVFLPLAHYMPTEQFNVW 189 A+ GE GA++ + EH+P GRRG ++++ G L+ V L + + F W Sbjct: 133 LALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTAVGEAAFADW 192 Query: 190 GWRIPFLLSFLVIIVCWIIRRKVDETPVFKAADQQELKRRSPVVEAFRDCTPNMLRVVCM 249 GWRIPFL+S L++ + IR ++E+P F + ++P+ EAF L++V + Sbjct: 193 GWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFGQW--KNLKLVIL 250 Query: 250 ALMNVIPVVATIFGAAYAVQPAYGVGFAKDVYLWIPVVGNIVAVLVIP-------YVGKL 302 AL+ + A ++ Y Q Y + F NI+ + + G L Sbjct: 251 ALVGLTAGQAVVW---YTGQ-FYALFFLTQTLKVDGPTANILIAAALLLATPFFLFFGSL 306 Query: 303 SDRIGRRLPIVVGSLL 318 SD+IGR+ PI++G L Sbjct: 307 SDKIGRK-PIILGGCL 321 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 163 bits (413), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 108/363 (29%), Positives = 185/363 (50%), Gaps = 11/363 (3%) Query: 22 IGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVARPIGAFVLGHLG 81 +G+ALE+YDF +Y +++I +LFFP + T+++ +LAT+G+G+ RP+G +LG Sbjct: 16 VGNALEWYDFIVYGFFSAII-ARLFFPADNEYTSLLVALATFGIGFFMRPVGGVLLGLYA 74 Query: 82 DTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVLLRLAQGFAVAGEISGASS 141 D GRK + + LM + + PTY +G+ AP ++V+ R+ QGFA GE S +++ Sbjct: 75 DRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFATGGEYSSSTA 134 Query: 142 MILEHSPFGRRGYYSSFALQGVQAGQILAAAVFLPLAHYMPTEQFNVWGWRIPFLLSFLV 201 ++E +P +RG Y S+ L G A + + + E + WGWR+PF+L L+ Sbjct: 135 FLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWRLPFVLGLLI 194 Query: 202 IIVCWIIRRKVDETPVFKAADQQELKRRSPVVEAFRDCTPNMLRVVCMALMNVIPVVATI 261 V IRR + ET F + + +P+ D LR V +++ VI Sbjct: 195 GPVGLWIRRHMHETDDFLESANKP--NAAPI--KLLDVVKTNLRGVLVSMGQVINGTVA- 249 Query: 262 FGAAYAVQPAYG---VGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIGRRLPIVVGSLL 318 F P + +G D + ++ + VIP+ G LSDR+GRR ++ SL Sbjct: 250 FYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLYSSLA 309 Query: 319 AGLFAFA-YLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFFPELFPARARVSAMAIG 377 L + +++ + ++ VM +L+ V+ + + P+ E FP R R + +A+ Sbjct: 310 FLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPM-PTALSEQFPTRVRSTGLAVA 368 Query: 378 QNL 380 NL Sbjct: 369 YNL 371 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,391,038 Number of extensions: 56513 Number of successful extensions: 318 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 283 Number of HSP's successfully gapped: 14 Length of query: 465 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 376 Effective length of database: 1,198,455 Effective search space: 450619080 Effective search space used: 450619080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)