BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3657.p (436 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313041|ref|YP_003777133.1| major facilitator superfamily p... 837 0.0 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 204 9e-54 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 191 8e-50 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 183 3e-47 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 167 1e-42 >gi|300313041|ref|YP_003777133.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 436 Score = 837 bits (2162), Expect = 0.0, Method: Compositional matrix adjust. Identities = 416/436 (95%), Positives = 430/436 (98%) Query: 1 MTTSAEFTGALNESQHRPLTSSDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADM 60 MTTSAEFTGALNESQHRPL+SSDY+TLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADM Sbjct: 1 MTTSAEFTGALNESQHRPLSSSDYKTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADM 60 Query: 61 PEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTY 120 PEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTY Sbjct: 61 PEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTY 120 Query: 121 ASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLG 180 ASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLG Sbjct: 121 ASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLG 180 Query: 181 SLMGIFINSVYQPAEVAAYAWRIPFLLGGVFGLVSVYLRRYLEETPVFRELAARRKQSHE 240 SLMGIFINS ++PAEVA YAWRIPF+LGGVFGLVSVYLRRYLEETPVFRELAARRK+++E Sbjct: 181 SLMGIFINSAFEPAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKEANE 240 Query: 241 LPLKTVLREHRGASLLTAGMTWCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLM 300 LPLKTVLREHR AS+LTAGMTWCLSGAVVVVILMTPSYLQTVHHL KLVMQANCAGTLM Sbjct: 241 LPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLSPKLVMQANCAGTLM 300 Query: 301 LTIGCLLIGWLCDRIGTRASMILGWGGLGLSSWWFYGQLPGSPESLIWGYALVGLFVGSI 360 LTIGCL IGWLCDRIGTRASMI+GWGGLGLS+WWFYGQLPGSPESL+WGYALVGLFVGSI Sbjct: 301 LTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESLVWGYALVGLFVGSI 360 Query: 361 ALTPIVSTRAFPPPVRFTGLSFAYNMAYAIFGGLTPVLISLWIKADPQGPALYVIGLSIL 420 +LTPIVSTRAFPPPVRFTGLSFAYNMAYA+FGGLTPVLISLWIKADPQGPALYVIGLSIL Sbjct: 361 SLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSIL 420 Query: 421 GVLLAFVPMSSKGYRA 436 GVLLAFVPMSSKGYRA Sbjct: 421 GVLLAFVPMSSKGYRA 436 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 204 bits (520), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 142/419 (33%), Positives = 218/419 (52%), Gaps = 33/419 (7%) Query: 10 ALNESQHRPLTSSDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPA--DMPEWLRML 67 +++ Q R R + + +G LE++DF +Y FFA ++ KLFFP D+ L + Sbjct: 13 SVSNQQRR-------RAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAV 65 Query: 68 QTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAA 127 TFG+ G+ RPVGGI++ Y D +GRK +++I LMAV T +IGL PTY SIG+ A Sbjct: 66 ATFGV---GFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFA 122 Query: 128 PILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFI 187 P+++++ R LQG + GGEM A F+ E+ P ++ + A I +LLG+ +G F+ Sbjct: 123 PLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFV 182 Query: 188 NSVYQPAEVAAYAWRIPFLLGGVFGLVSVYLRRYLEETPVFRELAARRKQSHELPLKTVL 247 + A +A++ WR+PFLLG + G V Y+R +LEETP F E K + PLK V+ Sbjct: 183 TASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLE----EKDKTDSPLKEVI 238 Query: 248 REHRGASLLTAGMTWCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLMLTIGCLL 307 R + + M + V++ P+Y V LP A G L + + + Sbjct: 239 RNFPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPI 298 Query: 308 IGWLCDRIGTRASMILGWGGLGLSSWWFYGQLPGSPE-------SLIWGYALVGLFVGSI 360 +G L DRIG R + + + ++ + + +P L++G L+ + G Sbjct: 299 VGRLADRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFG-TLIAAYTG-- 355 Query: 361 ALTPIVS--TRAFPPPVRFTGLSFAYNMAYAIFGGLTPVLISLWIKA--DPQGPALYVI 415 PI++ + FP V TGLS AYN+A IFGG I+ I A PA YV+ Sbjct: 356 ---PILAAFSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVM 411 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 191 bits (486), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 124/406 (30%), Positives = 201/406 (49%), Gaps = 7/406 (1%) Query: 22 SDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQTFGIFAAGYLARP 81 + R + + +G LE+YDF++Y FF+A++ +LFFPAD E+ +L F G+ RP Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPAD-NEYTSLLVALATFGIGFFMRP 64 Query: 82 VGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMRTLQGAA 141 VGG+++ Y D GRK + I LM + +I PTYA+IG+AAPI++++ R LQG A Sbjct: 65 VGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFA 124 Query: 142 IGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFINSVYQPAEVAAYAW 201 GGE + F+ E PA++ G + G+ MG + P + ++ W Sbjct: 125 TGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGW 184 Query: 202 RIPFLLGGVFGLVSVYLRRYLEETPVFRELAARRKQSHELPLKTVLREHRGASLLTAGMT 261 R+PF+LG + G V +++RR++ ET F E +A + + + L V++ + L++ G Sbjct: 185 RLPFVLGLLIGPVGLWIRRHMHETDDFLE-SANKPNAAPIKLLDVVKTNLRGVLVSMGQV 243 Query: 262 WCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLMLTIGCLLIGWLCDRIGTRASM 321 + A VV++ P++ LP +LT G L DR+G R + Sbjct: 244 INGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVL 303 Query: 322 ILGWGGLGLSSWWFY---GQLPGSPESLIWGYALVGLFVGSIALTPIVSTRAFPPPVRFT 378 + L + + P P L+ L + + P + FP VR T Sbjct: 304 LYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRST 363 Query: 379 GLSFAYNMAYAIFGGLTPVLISLWIKA--DPQGPALYVIGLSILGV 422 GL+ AYN+A +FGG P +++ K P PA YV+ + +GV Sbjct: 364 GLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGV 409 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 183 bits (464), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 132/407 (32%), Positives = 203/407 (49%), Gaps = 14/407 (3%) Query: 25 RTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQTFGIFAAGYLARPVGG 84 R + S++G LE++D +IY FA V+ K FFP + + +L TF F + RP+G Sbjct: 19 RAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTG-DDSVSLLLTFATFGVSFFMRPLGA 77 Query: 85 IIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMRTLQGAAIGG 144 +++ Y D GRK LSI LM V T +I +P+YASIG+ AP + L + +QG + GG Sbjct: 78 VVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGG 137 Query: 145 EMPGAWVFVAEHVPANRYSFGVGSL-TAGITGGILLGSLMGIFINSVYQPAEVAAYAWRI 203 E + F+ EH P R F + + GI+ +LL +L G +N++ P ++A++ WR+ Sbjct: 138 EFGSSTAFLVEHAPHRRGFFSSWQVASQGIS--LLLAALFGAVLNNMLTPEQLASWGWRV 195 Query: 204 PFLLGGVFGLVSVYLRRYLEETPVFRELAARRKQSHELPLKTVLREHRGASLLTAGMTWC 263 PF+ G + +Y+RR+L+E P F+E + PL+ H+ LL + Sbjct: 196 PFIFGLLIAPAGIYIRRHLDEAPEFKE----SSEKTNAPLRDTF-AHQKMRLLIGAGSVI 250 Query: 264 LSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLMLTIGCLLIGWLCDRIGTRASMIL 323 ++ V + L P+Y LPA A L++ IG L+G L D+IG MI Sbjct: 251 MATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMIT 310 Query: 324 G---WGGLGLSSWWFYGQLPGSPESLIWGYALVGLFVGSIALTPIVSTRAFPPPVRFTGL 380 + L + F PG + L+ + L A P + FP R TG+ Sbjct: 311 SSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGM 370 Query: 381 SFAYNMAYAIFGGLTPVLISLWIKADPQGPALYVIGLSILGVLLAFV 427 S AYN+A IFGG ++I+ I D G L V I G +L+ + Sbjct: 371 SLAYNIAVTIFGGFAGLIITWLI--DFTGDKLSVSYFVIFGAVLSLI 415 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 167 bits (424), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 125/406 (30%), Positives = 209/406 (51%), Gaps = 21/406 (5%) Query: 33 GGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQTFGIFAAGYLARPVGGIIIAHYGD 92 G LE +DF +Y F+A+ + K FFP+D E++ ++ TFG F AG+L RP+G II+ Y D Sbjct: 22 GNFLEMFDFFLYGFYASYISKTFFPSD-NEFVSLILTFGTFGAGFLMRPLGAIILGSYID 80 Query: 93 VIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMRTLQGAAIGGEMPGAWVF 152 +GR++ +++ +MA+ TL+I +P YA+IGVAAPIL+L R LQG + G E+ G V+ Sbjct: 81 RVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQGFSAGVELGGVSVY 140 Query: 153 VAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFINSVYQPAEVAAYAWRIPFLLGGVFG 212 +AE F V +A I+ +L+G ++ P +++ + WRIPF +G + Sbjct: 141 LAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDWGWRIPFFIGCMIV 200 Query: 213 LVSVYLRRYLEETPVFRELAARRKQSHELPLKTVLREHRGASLLTAGMTWCLSGAVVVVI 272 +RR L+ET E AR++ + + + ++ + M + + ++ Sbjct: 201 PAIFIIRRSLQETD---EFLARKRHPSFGEIMSSIAQNWKIVVAGMMMVVMTTVSFYLIT 257 Query: 273 LMTPSYLQTVHHLPAKLVMQAN-CAGTLMLTIGCLL--IGWLCDRIGTRASMILGWGGLG 329 + TP++ + V L A + C G L+ C L +G L DR+G R ++ Sbjct: 258 VYTPTFGKNVLKLSASDSLIVTFCVG---LSNFCWLPIMGALSDRVGRRPVLVAFTLLTL 314 Query: 330 LSSW----WFYGQLPGSPESLIWGYALVGLFVGSIALTPIVS-TRAFPPPVRFTGLSFAY 384 L+++ W + S ++L+ + S +V+ T P VR G S AY Sbjct: 315 LTAYPVMSWLVSDI--SFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRTVGFSLAY 372 Query: 385 NMAYAIFGGLTPVLISLWI---KADPQGPALYVIGLSILGVLLAFV 427 ++A AIFGG TP L+S W+ D P ++ ++ G+L V Sbjct: 373 SLATAIFGGFTP-LVSTWLIETSGDKAAPGYWMSFAAVCGLLATLV 417 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.143 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,366,069 Number of extensions: 57663 Number of successful extensions: 362 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 328 Number of HSP's successfully gapped: 15 Length of query: 436 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 348 Effective length of database: 1,203,190 Effective search space: 418710120 Effective search space used: 418710120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)