BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3656.p (523 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I M... 400 e-112 gi|300310584|ref|YP_003774676.1| methyl-accepting chemotaxis tra... 371 e-104 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 361 e-101 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 360 e-100 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 358 e-100 >gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I MCP-I serine chemoreceptor transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 556 Score = 400 bits (1027), Expect = e-112, Method: Compositional matrix adjust. Identities = 243/524 (46%), Positives = 346/524 (66%), Gaps = 6/524 (1%) Query: 4 NLTIKTRLITVIGFLLVISFLIGTTSLISLINVNNSLKTVYEDRLIALSQLDQV------ 57 +IK RLI I FL ++ G L+SL + N ++++YEDRL+A + L+Q+ Sbjct: 3 KFSIKIRLILAISFLTIMLLASGIVGLVSLHSTNEKMRSLYEDRLVAYAYLEQIESTLES 62 Query: 58 IRSSNKMQINIARALVSSQEELATHIASFKKEESVRDKVWKDYLATYLTPDEKALADTLV 117 RS I + + E+ + + V + Y ATYLTP+E L + L+ Sbjct: 63 ARSGIYGIITLDSSEGWDPNEIDRRLDLLVNNLKTVEPVIRSYQATYLTPEESTLLERLL 122 Query: 118 REQKLLDEKGFAPALNALNGGDMKTVATLIHGDIARYATSITETMNALIQLQTEVAKSEY 177 ++++ ++G PA+ +L + + G + + ++ +T++ L +LQ V + Y Sbjct: 123 KQRQHYADEGVMPAVESLRKRSFQGADDIYSGAMRKQWDAVHQTLSDLKELQINVGRELY 182 Query: 178 ENSQQRYQSFRSFSIAAIICSILVGSLIGLWLIRSINRPLNSAIEVAQRIASGDLTQSIE 237 E+ QQRY+ ++ A+ ++V L L+R+I++PL A+ +AQ +A GDL++ IE Sbjct: 183 EHEQQRYRQLILMALLAMGAGLIVAVLEAWLLVRAISQPLTQAVGIAQAVAGGDLSRQIE 242 Query: 238 VGGTNEMGRLLRALQEMSKALTQAVTQVRHSTDSITTASAEIASGNLDLSSRTEEQAASL 297 V +E G+L++AL++M++ L Q V VR T++IT AS EI SGNLDLS+RTE+QA SL Sbjct: 243 VRSQDETGQLMQALKDMTQRLYQIVAVVRQGTETITMASKEITSGNLDLSARTEQQAGSL 302 Query: 298 EQTASSMEELTSTVKQNADNALQANVLMAQARKQAENGGGIVNQVVATMNDIKQSSSHMV 357 E+TAS+M +LTSTVKQNADNA QAN L A A + A GG +V QVV TM I QSS+ +V Sbjct: 303 EETASAMAQLTSTVKQNADNASQANQLAASASEVAVRGGEVVRQVVDTMTSINQSSTRIV 362 Query: 358 DIITVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSASAAKEIRELIE 417 DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQR+ASAA+EI++LI+ Sbjct: 363 DIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRAASAAREIKDLID 422 Query: 418 ASLQKISSGGNLVNDAGAVMEETVDSVKRVAQLMEDIAAASQEQSNGIDQVNLAIGQMDQ 477 S++K+ G LV+ AG MEE V +V++V ++ +I++ASQEQS GI +++ AI MD Sbjct: 423 DSVEKVGKGSALVDQAGKTMEEVVTAVRKVTDMVGEISSASQEQSQGIQEIDRAIVLMDS 482 Query: 478 VTQQNAALVEEAAAAASSLRDQAQILTGAVSVFKTEQSHHAMTL 521 TQQNAALVE+AAAAA +L DQA+ L+ A+S F+ +H A L Sbjct: 483 STQQNAALVEQAAAAAQALEDQAEKLSLAMSAFRLGGAHSAERL 526 >gi|300310584|ref|YP_003774676.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 596 Score = 371 bits (952), Expect = e-104, Method: Compositional matrix adjust. Identities = 233/523 (44%), Positives = 329/523 (62%), Gaps = 31/523 (5%) Query: 4 NLTIKTRLITVIGFLLVISFLIGTTSLISL---INVNNSLKTVYEDRLIALSQLDQVIRS 60 NL+IK +L I L + IG + L+ + +++N L ++RL D + Sbjct: 5 NLSIKAQLAIAILVLAALLVAIGASGLLGMSAAVSINAQLS---QERLPKTVAADNTLIW 61 Query: 61 SNKMQINIARALVSSQEELATHIASFKKEESVRDKV---WKDYLATYLTPDEKAL----A 113 + + ++ +A +++ A + + + + R + W Y A T + + L + Sbjct: 62 IGRQRTSLDQAALTNDPAWAERM--YGMDANARKEALQWWGKYTALPQTEEGRVLVKKVS 119 Query: 114 DTLVREQKLLDEKGFAPALNALNGGDMKTVATLIHGDIARYATSITETMN----ALIQLQ 169 D + + ++ L FA + A GD + +A D AR +I M AL + + Sbjct: 120 DGIDKTEQELTR--FAEVIKA---GDRQAIA-----DQARKVGTIYTAMQLDGQALSKYE 169 Query: 170 TEVAKSEYENSQQRYQSFRSFSIAAIICSILVGSLIGLW-LIRSINRPLNSAIEVAQRIA 228 TE A S RY++ R+FSI AI+ +L+ + W L R+I P+ A+ IA Sbjct: 170 TEQAAQSAGASMARYEASRNFSIVAIVAGLLL-AFYSWWSLRRAIGEPMQRALRHFDAIA 228 Query: 229 SGDLTQSIEVGGTNEMGRLLRALQEMSKALTQAVTQVRHSTDSITTASAEIASGNLDLSS 288 SGDLT+++ V NEMG+LL L+ M ++L++ V VR +D+I+TA+ EIASGNLDLSS Sbjct: 229 SGDLTEAVHVHSRNEMGQLLEGLKRMQESLSRTVQAVRSGSDAISTATREIASGNLDLSS 288 Query: 289 RTEEQAASLEQTASSMEELTSTVKQNADNALQANVLMAQARKQAENGGGIVNQVVATMND 348 RTE+QAASLE+TASSMEELTSTVK NADNA QA L A A +G +V +VV TM + Sbjct: 289 RTEQQAASLEETASSMEELTSTVKHNADNARQATTLAVNASTIAADGNAVVGRVVQTMEE 348 Query: 349 IKQSSSHMVDIITVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSASA 408 I+ SS+ + DI+++IDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR LAQRS+SA Sbjct: 349 IRASSAKITDIVSIIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRALAQRSSSA 408 Query: 409 AKEIRELIEASLQKISSGGNLVNDAGAVMEETVDSVKRVAQLMEDIAAASQEQSNGIDQV 468 AKEI+ELI S+ ++ +G LV AG M + +++V+RV +M +I+AAS EQS GIDQV Sbjct: 409 AKEIKELIMHSVDRVQAGSELVGQAGDTMSDVINAVQRVTDIMSEISAASNEQSAGIDQV 468 Query: 469 NLAIGQMDQVTQQNAALVEEAAAAASSLRDQAQILTGAVSVFK 511 + A+ QMD+ TQQNAALVE+A+AAA SL QAQ L V++F+ Sbjct: 469 SHAVTQMDEATQQNAALVEQASAAAKSLEQQAQALMQEVAIFR 511 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 361 bits (926), Expect = e-101, Method: Compositional matrix adjust. Identities = 259/538 (48%), Positives = 335/538 (62%), Gaps = 48/538 (8%) Query: 1 MFANLTIKTRLITVIGFLLVISFL--IGTTSLISLINVNNSLKTVYEDRL---------- 48 F +L I +LIT FL+V++ + +G S+ L VN++ + + L Sbjct: 3 WFYDLRISRKLITT--FLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQ 60 Query: 49 IALS-----QLDQVIRSSNKMQINIARA-------LVSSQEELATHIASFKKEESVRDKV 96 +A+S +L ++ K + R L ++Q E T I S +E+++ +V Sbjct: 61 LAMSRLRSFELQHILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQI-SEPEEKAIYPEV 119 Query: 97 WKDYLATYLTPDEKALADTLVREQKLLDEKGFAPALNALNGGDMKTVATLIHGDIARYAT 156 K +AT+++ +K +A L R+ K DE+ A N G K Y T Sbjct: 120 EK-LIATFVSEHDKIIA--LSRQGK--DEE----ARNLQRGESTKA-----------YRT 159 Query: 157 SITETMNALIQLQTEVAKSEYENSQQRYQSFRSFSIAAIICSILVGSLIGLWLIRSINRP 216 S+ + ++ L + + A + + Y R + I + I + L+ +W+ R I RP Sbjct: 160 SLQQ-IDKLAAVNDKGADISNKQADAIYAQARLWIIVTVAACIGLAMLLAVWVSRLIARP 218 Query: 217 LNSAIEVAQRIASGDLTQSIEVGGTNEMGRLLRALQEMSKALTQAVTQVRHSTDSITTAS 276 L A+ VAQR+A GDLT IE G +E G+LL AL+ M+ +L V QVR T +ITTAS Sbjct: 219 LEEAVSVAQRVAGGDLTTRIEPAGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTAS 278 Query: 277 AEIASGNLDLSSRTEEQAASLEQTASSMEELTSTVKQNADNALQANVLMAQARKQAENGG 336 EIASGNLDLSSRTEEQA+SLE+TAS+MEELTSTVKQNADNA QAN L A + A GG Sbjct: 279 TEIASGNLDLSSRTEEQASSLEETASAMEELTSTVKQNADNARQANQLAVSASEVASQGG 338 Query: 337 GIVNQVVATMNDIKQSSSHMVDIITVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS 396 +VNQVV TM I SS + DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS Sbjct: 339 AVVNQVVDTMGSINASSRKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS 398 Query: 397 EVRGLAQRSASAAKEIRELIEASLQKISSGGNLVNDAGAVMEETVDSVKRVAQLMEDIAA 456 EVR LAQRSA AAKEI+ELIE S+ ++ G LV AG M E V SV+RV ++ +I++ Sbjct: 399 EVRSLAQRSAGAAKEIKELIEGSVAQVDMGSKLVERAGITMSEVVQSVRRVTDIVGEISS 458 Query: 457 ASQEQSNGIDQVNLAIGQMDQVTQQNAALVEEAAAAASSLRDQAQILTGAVSVFKTEQ 514 ASQEQS GI+QVNLAI QMD+VTQQNAALVEEAAAAA SL++QA+ LT V FK EQ Sbjct: 459 ASQEQSEGIEQVNLAIAQMDEVTQQNAALVEEAAAAAQSLQEQAETLTQTVGFFKLEQ 516 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 360 bits (924), Expect = e-100, Method: Compositional matrix adjust. Identities = 246/525 (46%), Positives = 343/525 (65%), Gaps = 24/525 (4%) Query: 2 FANLTIKTRLITVIGFLLVISFLIGTTSLISLINVNNSLKTVYEDRLIALSQLDQVIRSS 61 F NL I +LI ++V++ ++G ++ L VN + + + L I+++ Sbjct: 4 FNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWL-------PTIKAA 56 Query: 62 NKMQINIARALVSSQEELATHIASFKKEESVRD-KVWKDYLATYLTPDEKALADTLVREQ 120 +Q ++ R +S + ++ T ++E++++ K D LA E +++ E+ Sbjct: 57 QSIQASLPRMRIS-ELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEA---EE 112 Query: 121 KLLDEK---GFAPALNALNG-------GDMKTVATLIHGDIARYATSITETMNALIQLQT 170 K+L EK FA L G + L GD + S+ E M+ +I++ Sbjct: 113 KVLYEKFSKSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVND 172 Query: 171 EVAKSEYENSQQRYQSFRSFSIAAIICSILVGSL-IGLWLIRSINRPLNSAIEVAQRIAS 229 +K + ++Q Y+S + I ++ +I+V +L + + + R+++RPLN A+ VA+R+A Sbjct: 173 AGSKRSDDMAEQVYRSA-TLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQ 231 Query: 230 GDLTQSIEVGGTNEMGRLLRALQEMSKALTQAVTQVRHSTDSITTASAEIASGNLDLSSR 289 GDLT I T+E G L+ +L+ M+ +L Q V +VR TD+ITTAS EIA+GNLDLSSR Sbjct: 232 GDLTMDIRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSR 291 Query: 290 TEEQAASLEQTASSMEELTSTVKQNADNALQANVLMAQARKQAENGGGIVNQVVATMNDI 349 TEEQA SLE+TAS+MEELT+TVKQNADNA QAN L A A A GG +V QVV TM I Sbjct: 292 TEEQAGSLEETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGI 351 Query: 350 KQSSSHMVDIITVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSASAA 409 +SS+ + DII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSASAA Sbjct: 352 TESSNRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAA 411 Query: 410 KEIRELIEASLQKISSGGNLVNDAGAVMEETVDSVKRVAQLMEDIAAASQEQSNGIDQVN 469 KEI++LI+ S++K+ +G LV AGA M E V SV+RV ++ +I+AAS EQS GI+++N Sbjct: 412 KEIKQLIDDSVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEIN 471 Query: 470 LAIGQMDQVTQQNAALVEEAAAAASSLRDQAQILTGAVSVFKTEQ 514 AI QMD+VTQQNAALVEEAAAAA SL+DQA L+ VSVFK ++ Sbjct: 472 RAITQMDEVTQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKLQR 516 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 358 bits (918), Expect = e-100, Method: Compositional matrix adjust. Identities = 204/366 (55%), Positives = 267/366 (72%) Query: 146 LIHGDIARYATSITETMNALIQLQTEVAKSEYENSQQRYQSFRSFSIAAIICSILVGSLI 205 L +GD A+ S+ ++A++++ T + E + ++ + + + + ++L+ SL+ Sbjct: 148 LFNGDSAKLYRSMVGNLDAIVKINTSGSAQADEEASTVFRGAQRWIVGLLAATVLIASLL 207 Query: 206 GLWLIRSINRPLNSAIEVAQRIASGDLTQSIEVGGTNEMGRLLRALQEMSKALTQAVTQV 265 + +++RPL A+++AQR+A GDLT I G +E G L+ AL+ M+++L V++V Sbjct: 208 AFLVATNVSRPLKEAVQIAQRVARGDLTLRIRPAGRDETGALMEALRAMNESLRDIVSEV 267 Query: 266 RHSTDSITTASAEIASGNLDLSSRTEEQAASLEQTASSMEELTSTVKQNADNALQANVLM 325 R TD+ITTAS EIA GNLDLS+RTE+QA SLE+TAS+MEELTSTV QNADNA QAN L Sbjct: 268 RQGTDTITTASHEIARGNLDLSNRTEQQAGSLEETASAMEELTSTVTQNADNARQANQLA 327 Query: 326 AQARKQAENGGGIVNQVVATMNDIKQSSSHMVDIITVIDGIAFQTNILALNAAVEAARAG 385 A A A GG +V QVV TM I +SS + DII+VIDGIAFQTNILALNAAVEAARAG Sbjct: 328 ATASDIASQGGEVVGQVVQTMEGITESSRRIADIISVIDGIAFQTNILALNAAVEAARAG 387 Query: 386 EQGRGFAVVASEVRGLAQRSASAAKEIRELIEASLQKISSGGNLVNDAGAVMEETVDSVK 445 EQGRGFAVVASEVR LAQRSA AAKEI+ELI+ S+QK+S+G LV AG+ M E V SV+ Sbjct: 388 EQGRGFAVVASEVRSLAQRSAGAAKEIKELIDDSVQKVSAGSALVERAGSTMTEVVASVR 447 Query: 446 RVAQLMEDIAAASQEQSNGIDQVNLAIGQMDQVTQQNAALVEEAAAAASSLRDQAQILTG 505 RV ++ +I+AA+QEQS GI++VNLAI QMD+ TQQNAALVE+AAAAA SL+ QA L Sbjct: 448 RVTDVVAEISAATQEQSGGINEVNLAITQMDETTQQNAALVEQAAAAADSLKGQASNLAA 507 Query: 506 AVSVFK 511 V FK Sbjct: 508 VVGKFK 513 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.125 0.319 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,142,452 Number of extensions: 37442 Number of successful extensions: 562 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 257 Number of HSP's successfully gapped: 71 Length of query: 523 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 433 Effective length of database: 1,193,720 Effective search space: 516880760 Effective search space used: 516880760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)