BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3631.p (288 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxy... 529 e-152 gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herba... 140 1e-34 gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herba... 140 2e-34 gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase... 66 4e-12 >gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 296 Score = 529 bits (1363), Expect = e-152, Method: Compositional matrix adjust. Identities = 249/288 (86%), Positives = 270/288 (93%) Query: 1 MLNLNEDTITQAVLARHAGMSDERLRQIITSLVQHLHAFAREVKLTEAEWFEGIRFLTET 60 MLNLNEDTITQAVL+RHA ++D+RLR+I+TSLVQHLHAFAREVKLTE EWF GI FLTET Sbjct: 9 MLNLNEDTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTET 68 Query: 61 GQLCSDKRQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHVDNSPQYPLGADIAN 120 GQ+CSD+RQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHV+++PQYPLGADIAN Sbjct: 69 GQICSDQRQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHVEDAPQYPLGADIAN 128 Query: 121 GAKGQPCFVSGSVRGLDGKPIPHARMEVWQADEDGFYDVQYPDLDQLQARGTLHTDEQGR 180 GAKG+PCFVSGSVRG +G+P+ HARMEVWQADE+GFYDVQY DLDQ +ARGTL TD +GR Sbjct: 129 GAKGEPCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGR 188 Query: 181 YHFRSILANSYPIPHDGPVGKMLETLGRHPWRPAHLHFRIQAEGYETLITHVFRRGDPYL 240 YHFRSILAN YPIPHDGPVG+MLE LGRHPWRPAHLHFRIQAEGYETLITHVFRR D YL Sbjct: 189 YHFRSILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYL 248 Query: 241 ESDAVFGVRSTLIADWPRHAAGTAPDGTQCEQPFYTLDYDFILNPQRN 288 +SDAVFGVRSTLIADWP H AGTAPDGTQCEQPFYTLDYDF+LNP R Sbjct: 249 DSDAVFGVRSTLIADWPCHPAGTAPDGTQCEQPFYTLDYDFVLNPLRK 296 >gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 311 Score = 140 bits (352), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 89/274 (32%), Positives = 133/274 (48%), Gaps = 14/274 (5%) Query: 24 RLRQIITSLVQHLHAFAREVKLTEAEWFEGIRFLTETGQLCSDKRQEFILLSDTLGLSTL 83 R +QI+ ++ L ++++T E++ G+ ++ G E LLS LGL Sbjct: 29 RTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYINRLGTA-----HEAGLLSPGLGLDRF 83 Query: 84 VMAQQNHKPA-----GCTEATVFGPFHVDNSPQYPLGADIANGA--KGQPCFVSGSVRGL 136 + + + + A G T T+ GP +V +P A + +G+ GQ + G+V G Sbjct: 84 LDMRMDAEDAALGIAGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKSGQTIIMHGTVYGA 143 Query: 137 DGKPIPHARMEVWQADEDGFYDVQYPDLDQ--LQARGTLHTDEQGRYHFRSILANSYPIP 194 DGKP+P A++EVW A+ GFY P +Q R T+ TD QGRY FRSI+ +Y P Sbjct: 144 DGKPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPTAYGCP 203 Query: 195 HDGPVGKMLETLGRHPWRPAHLHFRIQAEGYETLITHVFRRGDPYLESDAVFGVRSTLIA 254 GP +L LGRH RPAH+HF + AEG+ L T + GDP + D + R L+ Sbjct: 204 PAGPTQGLLNALGRHGNRPAHIHFFVTAEGHRKLTTQINIEGDPLIYDDFAYATREGLVP 263 Query: 255 DWPRHAAGTAPDGTQCEQPFYTLDYDFILNPQRN 288 + E PF + +D L N Sbjct: 264 PLVERTDAASIKAEGLEGPFAEIVFDIKLTSLVN 297 >gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 140 bits (352), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 85/287 (29%), Positives = 137/287 (47%), Gaps = 11/287 (3%) Query: 7 DTITQAVLARHAGMSDERLRQIITSLVQHLHAFAREVKLTEAEWFEGIRFLTETGQLCSD 66 D + Q + + ++R++ ++ LV+ + E+ + E++ + +LT G Sbjct: 7 DAVLQKIESTELAEGNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSAG----- 61 Query: 67 KRQEFILLSDTLGLSTLVMAQQNHKPA-----GCTEATVFGPFHVDNSPQYPLGADIANG 121 K EF L++ LG + + + A G T T+ GP +V +P+ A + Sbjct: 62 KSGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDKD 121 Query: 122 AK-GQPCFVSGSVRGLDGKPIPHARMEVWQADEDGFYDVQYPDLDQLQARGTLHTDEQGR 180 + G F+ G V GKP+ +A +EVW A+ G Y PD R ++ TDEQGR Sbjct: 122 PQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDEQGR 181 Query: 181 YHFRSILANSYPIPHDGPVGKMLETLGRHPWRPAHLHFRIQAEGYETLITHVFRRGDPYL 240 Y FRS + Y +P G ++L LGRH RPAH+HF + GY L T + GDPYL Sbjct: 182 YRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPYL 241 Query: 241 ESDAVFGVRSTLIADWPRHAAGTAPDGTQCEQPFYTLDYDFILNPQR 287 D F R L+ + + + PFY ++++F L P++ Sbjct: 242 WDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQ 288 >gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase beta chain protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 65.9 bits (159), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 48/146 (32%), Positives = 65/146 (44%), Gaps = 25/146 (17%) Query: 113 PLGADIANGAKGQP----CFVSGSVRGLDGKPIPHARMEVWQADEDGFY----DVQYPDL 164 P D+ KG+P V+G V DGKP+ ++ +EVWQ + G Y D L Sbjct: 53 PHDMDLTKQGKGEPLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPL 112 Query: 165 D-QLQARGTLHTDEQGRYHFRSILANSYPIPHDGPVGKMLETLGRH--PWRPAHLHFRIQ 221 D G + TD+ GRY F SI YP G H WRPAH+HF + Sbjct: 113 DPNFTGFGKMLTDDNGRYRFVSIKPGPYP-------------WGNHHKAWRPAHIHFSLF 159 Query: 222 AEGY-ETLITHVFRRGDPYLESDAVF 246 Y + L+T ++ DP D +F Sbjct: 160 GNVYAQRLVTQMYFPNDPLFAYDPIF 185 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 980,090 Number of extensions: 43318 Number of successful extensions: 87 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 84 Number of HSP's successfully gapped: 4 Length of query: 288 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 204 Effective length of database: 1,222,130 Effective search space: 249314520 Effective search space used: 249314520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)