BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3615.p (586 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313000|ref|YP_003777092.1| AMP-binding enzyme family prote... 913 0.0 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 59 1e-09 >gi|300313000|ref|YP_003777092.1| AMP-binding enzyme family protein [Herbaspirillum seropedicae SmR1] Length = 586 Score = 913 bits (2359), Expect = 0.0, Method: Compositional matrix adjust. Identities = 458/584 (78%), Positives = 503/584 (86%), Gaps = 2/584 (0%) Query: 5 AAP-LDLLQLPFQAFDAARVIGWRDGEAVTHAAWRCELLRWKQLLQETPGQRFALFHHDT 63 AAP LDLLQLPF AF A VIGWRDG AVTHA+WR EL RW+ L+Q TPGQR ALFHHDT Sbjct: 3 AAPRLDLLQLPFLAFAGADVIGWRDGVAVTHASWRAELRRWQHLVQTTPGQRMALFHHDT 62 Query: 64 LHFAAALFAAWLAGKTTYLPSDALPETCRQLQGEVDGFLGQFGSHCPALHPTEDAPHEQE 123 L FAAALFA WLAGKT YLPSDALP+TCRQLQGEVD FLG+FG+HCPALHP DA Sbjct: 63 LQFAAALFAIWLAGKTAYLPSDALPDTCRQLQGEVDAFLGEFGAHCPALHPAGDAAEGDT 122 Query: 124 AAPWPALRGDFPALVVYTSGSTGAAQAIPKQLAQMANEVATLETLFGARLGQADIISTVS 183 WP LR DF LVVYTSGSTG AQAIPKQL QMANEVATLE LFGA +G+A++I+TVS Sbjct: 123 PDAWPTLRADFGGLVVYTSGSTGLAQAIPKQLGQMANEVATLEALFGAIVGEAEVIATVS 182 Query: 184 HQHIYGLLFKVLWALCAGRPIQAGSAFFPEDLQRLVGQRPWVLLSSPAHLKRLPEQATQP 243 HQHIYGLLFKVLWALCAGRP+ AGSAFFPEDLQR+ G RPW+LLSSPAHLKRLPEQ QP Sbjct: 183 HQHIYGLLFKVLWALCAGRPVHAGSAFFPEDLQRMAGARPWILLSSPAHLKRLPEQGAQP 242 Query: 244 EVTQLRAIFSSGGPLPAEAARDTALTLGQRPLEIYGSSETGGIAWRQPESDPALRREHWQ 303 + QLRAIFSSGGPLPA+AA D+A LGQRPLEIYGSSETGGIAWRQP++DP R+EHWQ Sbjct: 243 DARQLRAIFSSGGPLPAQAAADSARLLGQRPLEIYGSSETGGIAWRQPQTDPLQRQEHWQ 302 Query: 304 VMPGVEVRIGASGELEVRSPHLPDRSWFGLADRAVLVEEIAGAFELRGRSDRIVKLEEKR 363 MPGVEVR+GA GELEVRSPHLPD +WFG+ADRA LV G FELRGRSDRIVKLEEKR Sbjct: 303 AMPGVEVRLGAEGELEVRSPHLPDSAWFGMADRAALVPGQPGQFELRGRSDRIVKLEEKR 362 Query: 364 ISLTRIEQLLAASPLVAEVRVIVHQGRRARQFIAAFVVPSASGRALLQAQGKLTLNGALK 423 ISLTRIEQLL ASPLV +VRVIVHQGRRARQFIAAFVVPSA A+L AQGKL LN ALK Sbjct: 363 ISLTRIEQLLNASPLVEDVRVIVHQGRRARQFIAAFVVPSAPAHAMLAAQGKLALNTALK 422 Query: 424 SVLAGAIEAIALPRLWRYLDAMPVNAQGKLTLSALTALLENPPPS-AARAPTLPRLTELQ 482 +VLAGAIE+IALPRLWRYL A+PVN QGK+TLSALTALLE+P + APTLP+++EL+ Sbjct: 423 AVLAGAIESIALPRLWRYLPALPVNPQGKVTLSALTALLESPAAAPVVSAPTLPQVSELE 482 Query: 483 RAADQVQLALEIPPDLLYFQGHFPGSPILPGVVQTDWSLLLGRRYFTLPPRFLGLNALKF 542 R+++ VQLAL+IP DLLYF+GHFPGSPILPGVVQTDWSL LGRRYF LPP FLGL ALKF Sbjct: 483 RSSEHVQLALDIPADLLYFRGHFPGSPILPGVVQTDWSLALGRRYFDLPPHFLGLQALKF 542 Query: 543 QRVITPGMRVLMDLHYDAAKAVLSFRLYSEGGQHASGRFQLGPA 586 QRVITPGMRVL+DL ++A K+ LSFRL+SEGGQHASGRF LG A Sbjct: 543 QRVITPGMRVLLDLQFEAGKSQLSFRLHSEGGQHASGRFLLGAA 586 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 58.9 bits (141), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 98/359 (27%), Positives = 143/359 (39%), Gaps = 62/359 (17%) Query: 137 LVVYTSGSTGAAQAIPKQLAQMANEVATLETLFGARLGQADIISTVSHQHIYGLLFKVLW 196 L++ TSG+TGAA+ +P L+ + + G L Q + YGL + ++ Sbjct: 201 LMMSTSGTTGAAKGVPVPLSALQAFSVYMREAVGL-LPQDKFWNMADPGWAYGLYYAIIG 259 Query: 197 ALCAGRPIQAGSAFFP-----EDLQRLVGQRPWVLLSSPAHLKRLPEQATQPEVT---QL 248 L G I A F E ++RL L +P + + + + +L Sbjct: 260 PLAIGHGITFNEAAFTVSGTYETIRRL---GITSLAGAPTAYRMMMAAGEEAAASVKGRL 316 Query: 249 RAIFSSGGPLPAEAARDTALTLGQRPLEIYGSSETGGIAWRQPESDPALRRE-----HWQ 303 RA+ S+G PL AE R A L + YG +E G + + ALR E Sbjct: 317 RAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGMVV----NNHHALRHEVVPGSAGF 372 Query: 304 VMPGVEV--------RIGAS--GELEV---RSP----------HLP--DRSWFGLADRAV 338 MPG V +GA+ GEL + RSP P ++ D Sbjct: 373 AMPGYRVVVLDENHQELGANQPGELAIDIARSPLYWFSGYWKQDTPAIAHGYYSTGDNVE 432 Query: 339 LVEEIAGAFELRGRSDRIVKLEEKRISLTRIEQLLAASPLVAEVRVIVHQGRRARQFIAA 398 L E G+ GRSD ++ RI +E L P VA+V VI + + A Sbjct: 433 L--EPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGLPDPERTEIVKA 490 Query: 399 FVVPSASGRALLQAQGKLTLNGALKSVLAGAIE----AIALPRLWRYLDAMPVNAQGKL 453 FVV S +A + AL+ LA ++ A A PR +LDA+P GKL Sbjct: 491 FVVLSDQFKA----------SDALREQLAQHVKRRLSAHAYPRAIEFLDALPKTPSGKL 539 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,915,289 Number of extensions: 85396 Number of successful extensions: 347 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 349 Number of HSP's successfully gapped: 2 Length of query: 586 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 496 Effective length of database: 1,193,720 Effective search space: 592085120 Effective search space used: 592085120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)