BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3594.p (459 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312979|ref|YP_003777071.1| UDP-glucose 6-dehydrogenase [He... 894 0.0 gi|300311320|ref|YP_003775412.1| UDP-glucose 6-dehydrogenase [He... 669 0.0 gi|300312048|ref|YP_003776140.1| UDP-N-acetyl-D-mannosaminuronat... 95 1e-20 gi|300313478|ref|YP_003777570.1| NDP-N-acetyl-D-mannosaminuronat... 82 9e-17 >gi|300312979|ref|YP_003777071.1| UDP-glucose 6-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 894 bits (2310), Expect = 0.0, Method: Compositional matrix adjust. Identities = 434/459 (94%), Positives = 445/459 (96%) Query: 1 MKITIIGTGYVGLVTGACLAELGNDVFCLDVDQRKVDILNNGGIPIHEPGLEEVVARNRA 60 MKITIIGTGYVGLVTGACLAELGNDVFCLDVDQRKVDILNNGGIPIHEPGLEEVV RNRA Sbjct: 1 MKITIIGTGYVGLVTGACLAELGNDVFCLDVDQRKVDILNNGGIPIHEPGLEEVVERNRA 60 Query: 61 AGRLQFSTDVAASVAHGDIQFIAVGTPPDEDGSADLQYVLAAARNIGRHMEGFKVVVDKS 120 AGRLQFSTDVAASVAHGDIQFIAVGTPPDEDGSADLQYVLAAARNIGR+MEGFKVVVDKS Sbjct: 61 AGRLQFSTDVAASVAHGDIQFIAVGTPPDEDGSADLQYVLAAARNIGRYMEGFKVVVDKS 120 Query: 121 TVPVGTADRVKAAIAAELATRGKTELSFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDQSP 180 TVPVGTADRVKAAIA ELA RGKTELSFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDQSP Sbjct: 121 TVPVGTADRVKAAIADELAQRGKTELSFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDQSP 180 Query: 181 EGLRAQAQMKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLADRVG 240 EGL AQ MKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLAD+VG Sbjct: 181 EGLHAQTLMKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLADKVG 240 Query: 241 ADIESVRHGIGSDPRIGHSFLYAGCGYGGSCFPKDVQALERTARDYDQSLLILRAVEQVN 300 ADIESVRHGIGSDPRIGHSFLYAGCGYGGSCFPKDVQALERTARDYDQSLLILRAVEQVN Sbjct: 241 ADIESVRHGIGSDPRIGHSFLYAGCGYGGSCFPKDVQALERTARDYDQSLLILRAVEQVN 300 Query: 301 DLQKHVLGKKVVNRFGDDLSGKHFAIWGLAFKPNTDDMREASSRVLIGELVRRGASVAVY 360 DLQKHVLGKKVV RFG+DL+GKHFA+WGLAFKPNTDDMREASSRVLIGELVRRGA+VAVY Sbjct: 301 DLQKHVLGKKVVARFGEDLAGKHFALWGLAFKPNTDDMREASSRVLIGELVRRGATVAVY 360 Query: 361 DPVAMKEAARVLELDFADDASLLQRIRFATSPMDTLQGADALAIVTEWKAFRSPDLERVK 420 DPVAMKEA+RVL+LDFADDA+LLQRIRF TSPMDTLQ ADALAIVTEWKAFRSPD ERVK Sbjct: 361 DPVAMKEASRVLQLDFADDAALLQRIRFGTSPMDTLQDADALAIVTEWKAFRSPDFERVK 420 Query: 421 AALNNPVIFDGRNLFDPQVMADSGVEYYGIGRSLPARVQ 459 A L NPVIFDGRNLFDPQ+MADSG+EYYGIGRSL AR Q Sbjct: 421 ATLKNPVIFDGRNLFDPQLMADSGIEYYGIGRSLLARGQ 459 >gi|300311320|ref|YP_003775412.1| UDP-glucose 6-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 669 bits (1727), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/452 (71%), Positives = 374/452 (82%) Query: 1 MKITIIGTGYVGLVTGACLAELGNDVFCLDVDQRKVDILNNGGIPIHEPGLEEVVARNRA 60 M IT+IGTGYVGLVTGACLAELGN VFCLDVD+ K++ILN+GG+PI+EPGL+E++ARNRA Sbjct: 1 MNITVIGTGYVGLVTGACLAELGNKVFCLDVDRNKIEILNSGGVPIYEPGLKEIIARNRA 60 Query: 61 AGRLQFSTDVAASVAHGDIQFIAVGTPPDEDGSADLQYVLAAARNIGRHMEGFKVVVDKS 120 +GRL FSTDVAASVAHG++QFIAVGTPPDEDGSADLQYV+AAARNIGRHME +VVDKS Sbjct: 61 SGRLIFSTDVAASVAHGEVQFIAVGTPPDEDGSADLQYVVAAARNIGRHMERNCIVVDKS 120 Query: 121 TVPVGTADRVKAAIAAELATRGKTELSFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDQSP 180 TVPVGT ++VKAAI AEL RG+ EL +VSNPEFLKEGAAVEDFMRPDRIVIG ++ Sbjct: 121 TVPVGTGEKVKAAIRAELEARGRGELDAMIVSNPEFLKEGAAVEDFMRPDRIVIGVEEGA 180 Query: 181 EGLRAQAQMKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLADRVG 240 G A+ M++LYAP NRNHER +MDV+SAEFTKYAANAMLATRISFMNELANLADR+G Sbjct: 181 AGAEAREVMRELYAPLNRNHERILYMDVKSAEFTKYAANAMLATRISFMNELANLADRLG 240 Query: 241 ADIESVRHGIGSDPRIGHSFLYAGCGYGGSCFPKDVQALERTARDYDQSLLILRAVEQVN 300 DIE VR GIGSDPRIG SFLYAG GYGGSCFPKDVQAL RTA ++ Q L IL +VE VN Sbjct: 241 VDIEHVRKGIGSDPRIGFSFLYAGTGYGGSCFPKDVQALARTASEHGQELEILGSVESVN 300 Query: 301 DLQKHVLGKKVVNRFGDDLSGKHFAIWGLAFKPNTDDMREASSRVLIGELVRRGASVAVY 360 D QK VL KK+V R G+DLSGK FA+WGLAFKPNTDDMR A SRV++ EL++RGA V VY Sbjct: 301 DKQKQVLLKKIVARLGEDLSGKTFAVWGLAFKPNTDDMRAAPSRVIVRELLQRGAKVQVY 360 Query: 361 DPVAMKEAARVLELDFADDASLLQRIRFATSPMDTLQGADALAIVTEWKAFRSPDLERVK 420 DPVAM+EA LD AD + +A M+ L+GADA+ IVTEWK+F+SPD VK Sbjct: 361 DPVAMEEARHAFALDLADLPGKEADLHYAGGAMEALEGADAVVIVTEWKSFKSPDFAAVK 420 Query: 421 AALNNPVIFDGRNLFDPQVMADSGVEYYGIGR 452 AAL P++FDGRNLF+P MA GVEY+ IGR Sbjct: 421 AALKEPLVFDGRNLFEPVTMARLGVEYHAIGR 452 >gi|300312048|ref|YP_003776140.1| UDP-N-acetyl-D-mannosaminuronate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 94.7 bits (234), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 103/366 (28%), Positives = 151/366 (41%), Gaps = 21/366 (5%) Query: 3 ITIIGTGYVGLVTGACLAELGNDVFCLDVDQRKVDILNNGGIPIHEPGLEEVVARNRAAG 62 + ++G GYVGL E G + D + I N +P E A RAA Sbjct: 4 VAVVGLGYVGL---PLAVEFGKKRATVGFDLSRSKIENYRRFV--DPTGEVSSAELRAAS 58 Query: 63 RLQFSTDVAASVAHGDIQFIAVGTPPDEDGSADLQYVLAAARNIGRHMEGFKVVVDKSTV 122 +L STD A +A D +AV TP DE + D + A++ +G+HM+ +VV +STV Sbjct: 59 QLTVSTD-PAILADADYIVVAVPTPVDEAHNPDFGPLAGASQTVGKHMKRGAIVVFESTV 117 Query: 123 PVGTADRVKAAIAAELATRGKTELSFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDQSPEG 182 G + V + E + K F V +PE + G +IV G D Sbjct: 118 YPGATEEVCVPL-LEKYSGLKWRDDFHVGFSPERINPGDKQHTLTNILKIVSGDDADTLE 176 Query: 183 LRAQAQMKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLADRVGAD 242 AQ + A +R ++ AE K N I+ MNEL+ + D++G D Sbjct: 177 RIAQLYESIITAGVHRASS------IKVAEAAKVIENTQRDLNIALMNELSIIFDKIGID 230 Query: 243 IESVRHGIGSDPRIGHSFLYAGCGY-GGSCFPKDVQALERTARDYDQSLLILRAVEQVND 301 V G+ +FL G GG C D L A ++ A ++ND Sbjct: 231 TLEVLQAAGTK----WNFLPFRPGLVGGHCIGVDPYYLTHKAEMLGYHPQVILAGRRIND 286 Query: 302 LQKHVLGKKVVNRF---GDDLSGKHFAIWGLAFKPNTDDMREASSRVLIGELVRRGASVA 358 + +K V G + G + GL FK N D+R + ++ EL G V Sbjct: 287 GMAKFVAEKTVKSMIAAGFPIKGSKVNVLGLTFKENCPDLRNSKVVDIVTELQSYGVEVH 346 Query: 359 VYDPVA 364 V+DPVA Sbjct: 347 VHDPVA 352 >gi|300313478|ref|YP_003777570.1| NDP-N-acetyl-D-mannosaminuronate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 424 Score = 82.0 bits (201), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 96/376 (25%), Positives = 154/376 (40%), Gaps = 25/376 (6%) Query: 1 MKITIIGTGYVGLVTGACLAELGND--VFCLDVDQRKVDILNNGGIPIHEPGLEEVVARN 58 +K+ +IG GYVGL E G V D++QR++D L +G E EE+ Sbjct: 6 IKLAVIGLGYVGLPLAV---EFGRKRPVIGFDINQRRIDELKSGNDFTLETTPEEL---- 58 Query: 59 RAAGRLQFSTDVAASVAHGDIQFIAVGTPPDEDGSADLQYVLAAARNIGRHMEGFKVVVD 118 AA +L F+T + + + V TP DE DL ++ A+ +G+ ++ +V+ Sbjct: 59 SAATKLSFTTSLE-DLRTCSCYIVTVPTPIDEYKRPDLPPLIKASETVGKVLKKGDIVIY 117 Query: 119 KSTVPVGTADRVKAAIAAELATRGKTELSFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDQ 178 +STV G + EL + K F +PE + G ++ G Sbjct: 118 ESTVYPGCTEE-DCVPVLELHSGLKFNEDFFCGYSPERINPGDKEHRVTTIKKVTSGSTP 176 Query: 179 SPEGLRAQAQMKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLADR 238 + + A ++ E +R AE K N I+ +NELA + ++ Sbjct: 177 AVADI-VDALYNEIIVAGTHKAE-----SIRVAEAAKVIENTQRDLNIALINELALIFNK 230 Query: 239 VGADIESVRHGIGSDPRIGHSFLYAGCGY-GGSCFPKDVQALERTARDYDQSLLILRAVE 297 +G D E+V GS +FL G GG C D L A+ I+ A Sbjct: 231 MGIDTEAVLKAAGSK----WNFLPFRPGLVGGHCIGVDPYYLTHKAQAIGYHPEIILAGR 286 Query: 298 QVND-LQKHVLGK--KVVNRFGDDLSGKHFAIWGLAFKPNTDDMREASSRVLIGELVRRG 354 ++ND + +V+ + K + + + G + G FK N D+R ++ EL Sbjct: 287 RLNDSMGTYVVAQLVKAMTKKRIQVEGAKVLVMGFTFKENCPDLRNTRVVDIVAELKDYN 346 Query: 355 ASVAVYDPVAMKEAAR 370 V VYDP E A Sbjct: 347 CEVDVYDPWVTTEEAE 362 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,439,570 Number of extensions: 60500 Number of successful extensions: 212 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 206 Number of HSP's successfully gapped: 4 Length of query: 459 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 371 Effective length of database: 1,203,190 Effective search space: 446383490 Effective search space used: 446383490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)