BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3554.p (415 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312932|ref|YP_003777024.1| nitrite/nitrate transporter pro... 752 0.0 gi|300312204|ref|YP_003776296.1| high affinity nitrate/nitrite t... 74 2e-14 gi|300312933|ref|YP_003777025.1| nitrite/nitrate transporter pro... 73 3e-14 gi|300313658|ref|YP_003777750.1| transport transmembrane protein... 59 6e-10 >gi|300312932|ref|YP_003777024.1| nitrite/nitrate transporter protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 752 bits (1941), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/411 (90%), Positives = 392/411 (95%) Query: 1 MSDQSRAYGVLGSSTLAFTVCFTIWVMFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLI 60 MSD +A GVL SSTLAFTVCFTIW+MFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLI Sbjct: 1 MSDPRKANGVLASSTLAFTVCFTIWMMFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLI 60 Query: 61 RVPLGIWTDRYGGRIVFFILMLCTVLPIWLISYATAFWHFLVLGLFVGLAGGSFSVGTPY 120 RVPLGIWTDRYGGRIVFF+LML TV+PIWLISYA AFWHFLVLGLFVGLAGGSFSVGTPY Sbjct: 61 RVPLGIWTDRYGGRIVFFLLMLSTVIPIWLISYAHAFWHFLVLGLFVGLAGGSFSVGTPY 120 Query: 121 VARWFKKERQGLAMGVFGAGNSGAAVNKFIAPALIAAGGWVLVPQVYAGLMLAAALIFWF 180 VARWFKKERQGLAMGVFGAGNSGAAVNKFIAP LIA+ GW LVPQVYAGLMLAAALIFW Sbjct: 121 VARWFKKERQGLAMGVFGAGNSGAAVNKFIAPVLIASAGWTLVPQVYAGLMLAAALIFWL 180 Query: 181 CSATNPSHQVASDTRFIDQLRMLKEPKVWRYCQYYSVVFGGYVGLSLWMVKYYVTEYGFD 240 SAT+PSHQV+SDTRF+DQ+RML++P+VWRYCQYYSVVFGGYVGLSLWMVKYYVTEYGFD Sbjct: 181 GSATDPSHQVSSDTRFMDQVRMLRDPRVWRYCQYYSVVFGGYVGLSLWMVKYYVTEYGFD 240 Query: 241 IQHAALLAACFSLPGGVLRAMGGWLSDKYGAYKVTWYVMWICWVAFFLLSYPQTDFTIQT 300 I+HAALLAACFSLPGGVLRA+GGWLSD++GAY+VTW+VMW+CWVAFFLLSYPQTDF IQT Sbjct: 241 IRHAALLAACFSLPGGVLRALGGWLSDRFGAYQVTWWVMWVCWVAFFLLSYPQTDFVIQT 300 Query: 301 VNGPQRFHIGLNPVLFTVLLFVVGVAMAVGKASVFKFISDDYSSNIGAVSGIVGLAGGLG 360 V GPQ FHIGLNPVLFT+LLFVVGVAMA+GKASVFKFISDDYSSNIGAVSGIVGLAGGLG Sbjct: 301 VGGPQAFHIGLNPVLFTILLFVVGVAMAIGKASVFKFISDDYSSNIGAVSGIVGLAGGLG 360 Query: 361 GFILPILFGIALDLTGVRSSCFMLLYGTVCCSLVWMHFQFRREPTPTSSDA 411 GFILPILFG+ALDLTGVRSSCFMLLYGTVC SLVWMHFQFRRE TSS A Sbjct: 361 GFILPILFGLALDLTGVRSSCFMLLYGTVCISLVWMHFQFRREYPQTSSVA 411 >gi|300312204|ref|YP_003776296.1| high affinity nitrate/nitrite transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 74.3 bits (181), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 79/292 (27%), Positives = 122/292 (41%), Gaps = 31/292 (10%) Query: 2 SDQSRAYGVLGSSTLAFTVCFTIWVMFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLIR 61 S Q RA+ + + +AF VCF W + IK HL + + V L+R Sbjct: 14 SPQMRAFHL---TWMAFFVCFFAWFACAPLMPVIKGEFHLTPGQIANINIAAVAVTILVR 70 Query: 62 VPLGIWTDRYGGRIVFFILMLCTVLPIWLISYATAFWHFLVLGLFVGLAGGSFSVGTPYV 121 + +G DR+G R + L+L +P+ +++A ++ FL L +G G SF + + Sbjct: 71 LIIGPMCDRFGPRKTYTGLLLAGAVPVIGVAFAQSYESFLFFRLLIGAVGASFVITQYHT 130 Query: 122 ARWFKKERQGLA-MGVFGAGNSGAAVNKFIAPALIA-------AGGW-----VLVPQVYA 168 + F G A G GN+G V + + P L+A + GW +LVP V Sbjct: 131 SVMFAPNVVGTANAATAGWGNAGGGVAQGVMPLLMAALLMLGVSQGWGWRAALLVPGV-- 188 Query: 169 GLMLAAALIFWF----CSATNPSHQVASDTRFIDQLR--------MLKEPKVWRYCQYYS 216 LML A ++W C N A+ R +VW Y Sbjct: 189 -LMLVMAALYWRFTQDCPEGNYDALRAAGVTIESGKRGGWASFRAAAANYRVWMLFITYG 247 Query: 217 VVFGGYVGLSLWMVKYYVTEYGFDIQHAALLAACFSLPGGVLRAMGGWLSDK 268 FG + + YYV + ++ A + AA F L RA+GG++SDK Sbjct: 248 ACFGVEIFIHNIAAIYYVEHFKLSLESAGIAAASFGLLALFARALGGFISDK 299 >gi|300312933|ref|YP_003777025.1| nitrite/nitrate transporter protein [Herbaspirillum seropedicae SmR1] Length = 460 Score = 73.2 bits (178), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 104/436 (23%), Positives = 172/436 (39%), Gaps = 62/436 (14%) Query: 20 VCFTIWVMFGVIGIPIKNA-LHLNETEFGLLTAMPVLTGSLIRVPLGIWTDRYGGRIVFF 78 + F +W+++ V+ + A H ++ + LTA+P L+G+ +R+ +GGR Sbjct: 37 LAFAVWMLWSVVAANLDKAGFHFSKDQLFFLTALPALSGATLRIFYSFLVPVFGGRKWTA 96 Query: 79 ILMLCTVLP-----IWLISYATAFWHFLVLGLFVGLAGGSFSVGTPYVARWFKKERQGLA 133 I ++P + L T + L L L GL GG+FS ++ +F K R+G A Sbjct: 97 ISTASLLIPAIGMGLALQDPTTRYSTLLGLALLCGLGGGNFSSSMANISFFFPKARKGYA 156 Query: 134 MGV-FGAGNSGAAVNKFIAPALIA--------AGGWVLVPQVYAGL-------------- 170 G+ G GN G +V +F+ P +IA +G L +GL Sbjct: 157 TGMNAGIGNLGVSVVQFVTPLVIASAMFGSAVSGQGFLFHDPKSGLESSFWLANAGFIWV 216 Query: 171 --MLAAALIFWFCSATNPSHQVASDTRFIDQLRMLKEPKVWRYCQYYSVVFGGYVGLSLW 228 ++AA + WF + ++ DQ + K W C Y FG ++G S Sbjct: 217 PFIIAATVAAWF----GMNDIASAHASLADQAVIFKRKHNWLMCWLYVGTFGSFIGFSAA 272 Query: 229 MVKYYVTEY-GFDIQHAALLAACFSLPGGVLRAMGGWLSDKYGAYKVTWYVMWICWVAFF 287 E+ + A L L G + R +GG +SD+ G +VT++V + Sbjct: 273 FAMLIKAEFPEIAVAQYAFLG---PLAGALARPLGGMISDRVGGARVTFWVFLAMGLIIL 329 Query: 288 LLSYPQTD--------------FTIQTVNGPQRFHIGLNPVLFTVLLFVVGVAMAVGKAS 333 L P F + + F + PV+F A+ + Sbjct: 330 LGILPALHTHQFMLFLASFIGMFILTGLGNGSTFC--MIPVIFQT---ERERAVQGQGEA 384 Query: 334 VFKFISDDYSSNIGAVSGIVGLAGGLGGFILPILFGIALDLTGVRSSC---FMLLYGTVC 390 + D AV G G G GGF +P FG ++ +TG F+ Y + C Sbjct: 385 AQQQARLDAGKESAAVLGFSGAIGAYGGFFIPQSFGTSIKMTGAPDMAMYVFIAFYAS-C 443 Query: 391 CSLVWMHFQFRREPTP 406 ++ W ++ R P P Sbjct: 444 MAITWWYYARRNAPMP 459 >gi|300313658|ref|YP_003777750.1| transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 59.3 bits (142), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 62/241 (25%), Positives = 106/241 (43%), Gaps = 9/241 (3%) Query: 35 IKNALHLNETEFGLLTAMPVLTGSLIRVPLGIWTDRYGGRIVFFILMLCTVLPIWLISYA 94 I + HL ++ G+L + L SL+++P G DRYG R V I +L L + A Sbjct: 39 IASEFHLKPSDLGILLSSFFLGYSLLQLPGGWLADRYGSRPVIVISILLWSLFTGVTGMA 98 Query: 95 TAFWHFLVLGLFVGLAGGSFSVGTPY-VARWF-KKERQGLAMGVFGAGNSGAAVNKFIAP 152 + +++ G+ G+F + VA F + ER ++ + + G+ + + Sbjct: 99 WSVTSLVMIRFIFGVGEGAFPAASVKGVAENFSRDERPKMSSLLMSSNYIGSMLAPLLIA 158 Query: 153 ALIAAGGWVLVPQVYAGLMLAAALIFWFC-SATNPSHQVASDTRFIDQLRMLKEPKVWRY 211 LI GW V + AL++WFC P A + ++K P +W Sbjct: 159 PLILHYGWRAVFHYIGIAGVVFALVYWFCVRPVRPGVGGAGAINKQAFMALMKMPLMW-- 216 Query: 212 CQYYSVVFGGYV---GLSLWMVKYYVTEYGFDIQHAALLAACFSLPGGVLRAMGGWLSDK 268 Q +V FG + GL WM Y +T G +++ LL + G+ A+GGW+ + Sbjct: 217 -QIVAVWFGLSIINKGLDTWMPTYLMTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWVMVR 275 Query: 269 Y 269 + Sbjct: 276 F 276 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.473 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,245,394 Number of extensions: 50871 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 305 Number of HSP's successfully gapped: 8 Length of query: 415 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 327 Effective length of database: 1,203,190 Effective search space: 393443130 Effective search space used: 393443130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)