BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3551.p (820 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrog... 1463 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 125 2e-29 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 97 4e-21 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 86 9e-18 >gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 824 Score = 1463 bits (3787), Expect = 0.0, Method: Compositional matrix adjust. Identities = 725/817 (88%), Positives = 756/817 (92%) Query: 1 MIIWLIVLIASTLALMLLRASAWAWLAAEVVLLWLGARLAGIELPVLVLLALLSLTPTLL 60 MIIWLI+LIAS LALM++RASAWAWLAAE VLLWLGARLA IE PVLVLLA+L TPTLL Sbjct: 1 MIIWLILLIASALALMMMRASAWAWLAAEAVLLWLGARLAEIEWPVLVLLAILFFTPTLL 60 Query: 61 VAIKSVRQSLMTRPALDLFRKIMPGMSATERDAIEAGTVWWDAELFSGKPDWRRLFELPA 120 VAI+SVRQ+LMTRPALD+FRKIMP MSATERDAIEAGTVWWDAELFSGKPDW RLF LPA Sbjct: 61 VAIRSVRQALMTRPALDIFRKIMPAMSATERDAIEAGTVWWDAELFSGKPDWSRLFALPA 120 Query: 121 PRLTPEEQAFLDNEVEQLCAMVSDWETSVKSQDLPPEAWQFIKDKGFLGMIIPRQYGGKQ 180 PRLT EEQAFLDNEVEQLC MVSDW+ +VK+QDLPPEAWQFIKDKGFLGMIIPRQYGGKQ Sbjct: 121 PRLTAEEQAFLDNEVEQLCGMVSDWDATVKTQDLPPEAWQFIKDKGFLGMIIPRQYGGKQ 180 Query: 181 FSAYMHSQVVMKLGSRSSTAAIAVMVPNSLGPAELLLHYGTEAQKRHYLPRLAQGLEIPC 240 FSAYMHSQVVMKL SRSS AAIAVMVPNSLGPAELLLHYGTE QK HYLPRLAQGLEIPC Sbjct: 181 FSAYMHSQVVMKLASRSSAAAIAVMVPNSLGPAELLLHYGTEVQKNHYLPRLAQGLEIPC 240 Query: 241 FALTSPYAGSDAAAIPDIGIVCKGEHEGRETLGFRITWRKRYITLAPMATVLGLAFRVRD 300 FALTSPYAGSDAAAIPDIGIVCKG HEGRETLGFR+TW KRYITLAP+ATVLGLAFR D Sbjct: 241 FALTSPYAGSDAAAIPDIGIVCKGVHEGRETLGFRVTWSKRYITLAPIATVLGLAFRASD 300 Query: 301 PDGLLGGSAEPGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKDVFIPMEWVIGGAQ 360 PDGLLGG E GITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKDVFIPMEWVIGG Sbjct: 301 PDGLLGGEREAGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKDVFIPMEWVIGGVA 360 Query: 361 QVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSAYTATRRQFRIEIGKFEGVQEAL 420 QVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTS Y A RRQF+IEIGKFEGVQEAL Sbjct: 361 QVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSGYAAMRRQFKIEIGKFEGVQEAL 420 Query: 421 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYHVTERGRIVVNDGMDIIGGKGICM 480 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYH+TERGR+VVNDGMD+IGGKGICM Sbjct: 421 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYHITERGRMVVNDGMDVIGGKGICM 480 Query: 481 GPGNFLARAYQQVPIAITVEGANILTRCLIIFGQGAIRCHPYVLKELAAAADEDRQRALQ 540 GPGNFLAR YQQ+PIAITVEGANILTRCLIIFGQGAIRCHPYVLKELAAAADEDR+RA+Q Sbjct: 481 GPGNFLARVYQQIPIAITVEGANILTRCLIIFGQGAIRCHPYVLKELAAAADEDRERAVQ 540 Query: 541 EFDRLLFAHLSFVAANAARSLVGGLSGGWLLPASRHAGRATQSYVRAVVHLSSAFALLTD 600 EFDRLLF HLSFVAANAARSLVGGLSGGWLL A A AT+ Y RAVVHLSSAFALLTD Sbjct: 541 EFDRLLFGHLSFVAANAARSLVGGLSGGWLLAAPAQAAHATRRYYRAVVHLSSAFALLTD 600 Query: 601 VSMGVLGGTLKFRERISARLGDVLSQLYLVSAVLKRFEDEGRQEADLPYVDWSVQDALNC 660 SM VLGGTLKFRERISARLGDVLSQLYLVSA LKR+EDEGRQEADLPYVDWSVQ+ALN Sbjct: 601 ASMAVLGGTLKFRERISARLGDVLSQLYLVSAALKRYEDEGRQEADLPYVDWSVQEALNR 660 Query: 661 AQEAIIEVLHNFPNPFIAFLLRGLIFPLGRPYRKPSDVLGTCVAKTMQTPGESRNRLLAD 720 AQEAI+EVLHNFPNPF+AF LR IFPLG PYRKP+DVLGT VAK MQTPGESR RLLAD Sbjct: 661 AQEAIVEVLHNFPNPFVAFALRLAIFPLGLPYRKPADVLGTHVAKAMQTPGESRERLLAD 720 Query: 721 AYLPREGDPLACGEQAFVLTPVVQQLEHRLKQDIKDGKLPPMPQNLPELPAWSALALERS 780 AYLPR GDPLACGEQAF LTPVVQQLEHRLK DI++GKLPPMPQ+L EL AWSALALER Sbjct: 721 AYLPRAGDPLACGEQAFALTPVVQQLEHRLKPDIREGKLPPMPQSLLELTAWSALALERG 780 Query: 781 LISAQEREALDTFAHYAEQSVRVDDFAADFDRAEMLA 817 L+S QE+EALD FA Y QSV VDDFAADFDRAEM+A Sbjct: 781 LLSLQEKEALDAFALYGHQSVAVDDFAADFDRAEMVA 817 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 125 bits (313), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 112/397 (28%), Positives = 181/397 (45%), Gaps = 37/397 (9%) Query: 123 LTPEEQAFLDN----EVEQLCAMVSDWETSVKSQDLPPEAWQFIKDKGFLGMIIPRQYGG 178 L+PE + D E+L +DW+ ++ P EA + + + G +GM +P Q+GG Sbjct: 3 LSPEHEMIRDAMRHFAQERLLPFAADWD---RNHTFPAEALKELAELGAMGMCVPEQWGG 59 Query: 179 KQFSAYMHSQVVMKLGSRSSTAAIAVMVPNSLGPAELLLHYGTEAQKRHYLPRLAQGLEI 238 + ++ + + V V NSL + YG+ QK +L LA+G + Sbjct: 60 AGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACG-ITEKYGSPTQKEQWLKPLARGEML 118 Query: 239 PCFALTSPYAGSDAAAIPDIGIVCKGEHEGRETLGFRITWRKRYITLAPMATVLGLAFRV 298 CF LT P+ GSDAAAI + E +G + F + K++IT A V + F V Sbjct: 119 GCFCLTEPHTGSDAAAIR-----TRAERDGDD---FILNGTKQFITSGKHAGV-AIVFAV 169 Query: 299 RDPDGLLGGSAEPGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKD-VFIPMEWVIG 357 D + + GI+C L+P D PG V GR + + D +P ++G Sbjct: 170 TDRS-----AGKKGISCFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLG 224 Query: 358 GAQQVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSAYTATRRQFRIEIGKFEGVQ 417 + G G+R+ + L AGR I + + SVG ++ A +Y R F + I + + V Sbjct: 225 ---KEGEGYRIALSNLEAGR-IGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVN 280 Query: 418 EALARMGGHLYMMDATRRL---SALAVDAGEKPSVISAISKYHVTERGRIVVNDGMDIIG 474 LA M ++DA R + +A D G ++++K +E + +D + I G Sbjct: 281 FRLADMN---TLLDAARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHG 337 Query: 475 GKGICMGPGNF-LARAYQQVPIAITVEGANILTRCLI 510 G G +F + R Y+ V I EGAN + R +I Sbjct: 338 GVGY---TSDFPVERIYRDVRICQIYEGANDIQRLVI 371 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 97.4 bits (241), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 103/400 (25%), Positives = 184/400 (46%), Gaps = 35/400 (8%) Query: 116 FELPAPRLTPEEQAFLDNEVEQLCAMVSDWETSVKSQDLPPEAWQFIKDKGFLGMIIPRQ 175 FEL +L ++ A D ++ + W+ P + + GF G+ P Sbjct: 3 FELNQDQLAFQQSA-RDFAAGEMAPFAAKWDEEAH---FPLDVIAKAGELGFCGLYTPEA 58 Query: 176 YGGKQFSAYMHSQVVMKLGSRSSTAAIAVMVPNSLGPAELLLHYGTEAQKRHYLPRLAQG 235 GG S + + VV + +R+ T+ A + +++ + ++ +G + K + +LA G Sbjct: 59 DGGLGLS-RLDATVVFEELARACTSTAAYLTIHNM-VSWMIASWGQPSLKAQWCEQLAAG 116 Query: 236 LEIPCFALTSPYAGSDAAAIPDIGIVCKGEHEGRETLGFRITWRKRYITLAPMATVLGLA 295 +I + LT P +GSDAA++ + G + + K +I+ A VL + Sbjct: 117 RKIGSYCLTEPGSGSDAASLKTTARLEDGH--------YVLNGSKAFISGAGSTDVLVVM 168 Query: 296 FRVRDPDGLLGGSAEPGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEG---KDVFIPM 352 R GG G++ ++P + PGV G++ + + + PT +V +P Sbjct: 169 ART-------GGEGARGVSAIVVPGNAPGVSYGKKESKMG--WNSQPTRTISFDNVKVPA 219 Query: 353 EWVIGGAQQVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSAYTATRRQFRIEIGK 412 + ++G Q G+G+ M+ L GR I++ + SVG ++ A+ AY R+QF I Sbjct: 220 DHLLG---QEGQGFIFAMKGLDGGR-INIATCSVGAAQAALDAAHAYMKERKQFGRPIAD 275 Query: 413 FEGVQEALARMGGHLYMMDATRRLSALAVDAGE-KPSVISAISKYHVTERGRIVVNDGMD 471 F+ +Q LA M L RL+A +DAG + + A++K T+ G V N+ + Sbjct: 276 FQALQFKLADMQTELVAARQMVRLAATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQ 335 Query: 472 IIGGKG-ICMGPGNFLARAYQQVPIAITVEGANILTRCLI 510 + GG G I P L R ++ V + +EG N + R +I Sbjct: 336 LHGGYGYIREYP---LERYFRDVRVHQILEGTNEIMRVII 372 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 86.3 bits (212), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 87/338 (25%), Positives = 145/338 (42%), Gaps = 32/338 (9%) Query: 150 KSQDLPPEAWQFIKDKGFLGMIIPRQYGGKQFSAYMHSQVVMKLGSRSSTAAIAVMVPNS 209 +S P + W+ + D G LG+ + +YGG Y+ + M+ SR+S + +S Sbjct: 39 RSDQFPMDLWKKLGDLGVLGITVSEEYGGAGL-GYLAHIIAMEEISRASASVGLSYGAHS 97 Query: 210 LGPAELLLHYGTEAQKRHYLPRLAQGLEIPCFALTSPYAGSDAAAIPDIGIVCKGEHEGR 269 + G E QKR YLPRL G I A++ P AGSD ++ + + +G Sbjct: 98 NLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAGSDVVSMK-----LRADKKGD 152 Query: 270 ETLGFRITWRKRYITLAPMATVLGLAFRVRDPDGLLGGSAEPGITCALIPTDHPGVVTGR 329 + + K +IT P A VL + + D + + G+T L+ + G + Sbjct: 153 R---YVLNGSKMWITNGPDADVL-VVYAKTDLE-----AGARGMTAFLVEKGYKGFSVAQ 203 Query: 330 RHWPLNAVFQNGPTEGKDVF----IPMEWVIGGAQQVGRGWRMLMECLAAGRAISLPSSS 385 + L+ + G G+ VF +P E V+GG VGRG +LM L R++ L Sbjct: 204 K---LDKLGMRGSHTGELVFQDCEVPEENVLGG---VGRGVNVLMSGLDFERSV-LSGGP 256 Query: 386 VGFSKMAVRGTSAYTATRRQFRIEIGKFEGVQEALARMGGHLYMMDATRRLSALAVDAGE 445 +G + + Y R+QF IG+F+ +Q LA M + A A D + Sbjct: 257 LGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRAD 316 Query: 446 KPSVISAISK------YHVTERGRIVVNDGMDIIGGKG 477 + A+ K + E+ + + + +GG G Sbjct: 317 SADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNG 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,436,449 Number of extensions: 106872 Number of successful extensions: 286 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 282 Number of HSP's successfully gapped: 4 Length of query: 820 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 727 Effective length of database: 1,179,515 Effective search space: 857507405 Effective search space used: 857507405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)