BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3530.p (702 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 1310 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 508 e-145 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 451 e-128 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 404 e-113 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 391 e-109 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 1310 bits (3390), Expect = 0.0, Method: Compositional matrix adjust. Identities = 626/700 (89%), Positives = 660/700 (94%) Query: 3 ALVRPPRLLETLLANLDGMVYRCRDDAHWTLEFVSDGCQALTGYTPADLLLNSRISFLQL 62 A+ R PRLLETLL NLDG+VYRCRDDA WT+EFVS+GC ALTGY P DLLLN R+SFL+L Sbjct: 2 AIPRSPRLLETLLVNLDGVVYRCRDDAQWTMEFVSEGCLALTGYLPEDLLLNQRLSFLEL 61 Query: 63 THPEDRAMVRKHIDACMRERRRIDIEYRIVHADGSLRWVWERGVGLYNAAGKVEAMEGFL 122 THPEDR +VR H+DACMRERRRIDIEYRIV ADG++RWVWERGVG+YNA G+VEA+EGFL Sbjct: 62 THPEDRTLVRNHVDACMRERRRIDIEYRIVRADGAVRWVWERGVGIYNANGQVEAIEGFL 121 Query: 123 QDVTERKEAAHALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLI 182 QDVTERKEAA ALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLI Sbjct: 122 QDVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLI 181 Query: 183 VGLRDITHQLYVEPERRAEFMRLMEREGSVSNFESRVYRRDGDIIWISENARAVYDDSGK 242 VGLRDI+HQLYVEPERR EFMRLME +GSVSNFESRVYRRDG+IIWISENARAVYD++GK Sbjct: 182 VGLRDISHQLYVEPERRQEFMRLMEEQGSVSNFESRVYRRDGEIIWISENARAVYDEAGK 241 Query: 243 LVCYEGTVEAITERKLYEAEMRHQATHDALTGLPNRNMLHEHLQRAIQVARQKGGLTAVV 302 L CYEGTVEAITERK +EAEMRHQATHDALTGLPNRNMLHEHLQRA++VARQKG LTAV Sbjct: 242 LSCYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVA 301 Query: 303 FVDLDQFKFINDSLGHQVGDELLKAVAQRLQACLRDTDMVARQGGDEFVLVLQNQTGGEL 362 FVDLDQFKFINDSLGHQVGDELLK VAQRLQACLR+ DMVARQGGDEFVLVLQNQT GE Sbjct: 302 FVDLDQFKFINDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEA 361 Query: 363 GIAEVMQRILAAVARPWQTGDRELQITASIGVSRYPADGKDVETLLKQADSAMYRAKEQG 422 GIAEVMQRILAAVARPWQ GDRE Q+TASIGVSRYPADGKDVE+LLK ADSAMYRAKEQG Sbjct: 362 GIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQG 421 Query: 423 RNNFQFFAPWMDTQVSNRLEMLINLRRALDHDEFKLYYQPKLSLKDGRVIGAEALIRWQS 482 RNNFQFFAPWMD+QVSNRLEMLINLRRALD DEFKLYYQPKLSL+DGR+IGAEALIRW+S Sbjct: 422 RNNFQFFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWES 481 Query: 483 PEQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACHQNKRWQQAGLPPIPVAVNLSPRQLNQ 542 P+QGMVPPDRFIPFAEEAGLIVPIGEWVLRTAC QN+ WQQAGLPPIPVAVNLSPRQLNQ Sbjct: 482 PQQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQ 541 Query: 543 SLPDFVSGVLAQSGLDAACLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYS 602 SLPDFV+GVLAQSGL A+ LELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYS Sbjct: 542 SLPDFVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYS 601 Query: 603 SLSYLRRFPVDALKIDKSFVRDIARDADSAAIVKAVISLAHILNLRVIAEGVEDEQQHAF 662 SLSYLRRFPVDALKIDKSFVRDI RDADSAAIVKA+ISLAHILNLRVIAEGVEDE+QHAF Sbjct: 602 SLSYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAF 661 Query: 663 LKENACDEVQGYYFGKPMAVEDFTAWMSRQGPTAQVLSCK 702 LKENACDEVQGYYFGKPM V FTAWMS Q QVLSCK Sbjct: 662 LKENACDEVQGYYFGKPMEVAVFTAWMSEQAQAGQVLSCK 701 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 508 bits (1307), Expect = e-145, Method: Compositional matrix adjust. Identities = 281/566 (49%), Positives = 368/566 (65%), Gaps = 11/566 (1%) Query: 124 DVTERKEAAHALQEAERRYRSI--FENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDL 181 D+ R+ A AL+ R+++I NAI + PD T VNPA ARI GY S E + Sbjct: 379 DIKARRLAERALR---LRHQAIEASANAILILSAQEPDYTIEYVNPAFARITGYSSTEAV 435 Query: 182 IVGLRDITHQLYVEPERRAEFMRLMEREGSVSNFESRVYRRDGDIIWISENARAVYDDSG 241 +R + + +PE E + + RE N R Y +DG +W V D+SG Sbjct: 436 GKSIRFLEGR-DSDPEG-FETVWIAMREQREHNVVLRNYHKDGSELWNDFYIAPVRDESG 493 Query: 242 KLVCYEGTVEAITERKLYEAEMRHQATHDALTGLPNRNMLHEHLQRAIQVARQKGGLTAV 301 ++ + + IT K YEAE+ QA+ D LTGL NR+ML HL++AI VA ++ V Sbjct: 494 EVTHFVAALYDITSMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWV 553 Query: 302 VFVDLDQFKFINDSLGHQVGDELLKAVAQRLQACLRDTDMVARQGGDEFVLVLQNQTGGE 361 VFVDLD+FKF+ND+LGH+ GD LL+ VA RL+A +R+ D VAR GGDEF+++L Q E Sbjct: 554 VFVDLDRFKFVNDTLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQD--E 611 Query: 362 LGI-AEVMQRILAAVARPWQTGDRELQITASIGVSRYPADGKDVETLLKQADSAMYRAKE 420 L + +QRI+ AVA P G L +T S+GV+ YP DG D ETL+K AD AMYR K+ Sbjct: 612 LTLDTRSLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQ 671 Query: 421 QGRNNFQFFAPWMDTQVSNRLEMLINLRRALDHDEFKLYYQPKLSLKDGRVIGAEALIRW 480 GRNNFQF+ P M+ Q RL + +LR ALDH EF L+YQPK+ L GRVIG+EALIRW Sbjct: 672 TGRNNFQFYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRW 731 Query: 481 QSPEQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACHQNKRWQQAGLPPIPVAVNLSPRQ- 539 Q P+ GMV P RFI AEE GLI+PIG WVLR AC Q ++WQ G I V+VNLS Q Sbjct: 732 QHPQLGMVSPARFIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQF 791 Query: 540 LNQSLPDFVSGVLAQSGLDAACLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGT 599 L + L ++ VL +GL LELE+TE+++M D E ++ L LK+LG+++S+DDFGT Sbjct: 792 LQKDLVSSIADVLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGT 851 Query: 600 GYSSLSYLRRFPVDALKIDKSFVRDIARDADSAAIVKAVISLAHILNLRVIAEGVEDEQQ 659 GYSSL+YL+RFP+D LKID+SFVRDI DAD AAI ++ISLA L LRVIAEGVE ++Q Sbjct: 852 GYSSLAYLKRFPIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQ 911 Query: 660 HAFLKENACDEVQGYYFGKPMAVEDF 685 +L+ CDE+QGY F P+ + F Sbjct: 912 LDYLRHYGCDEIQGYLFSPPVPADKF 937 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 451 bits (1160), Expect = e-128, Method: Compositional matrix adjust. Identities = 276/713 (38%), Positives = 409/713 (57%), Gaps = 47/713 (6%) Query: 1 VTALVRPPRLLETLLANLDGMVYRCRDDAH--------WTLE----FVSDGCQALTGYTP 48 V++ VR RL + + A L + R A W L+ +++ TGYT Sbjct: 329 VSSRVRAVRLAQKMTAELRASEEQFRAIADCTVNWEVWWGLDGRPRWINHAVLDYTGYTV 388 Query: 49 ADLL-LNSRISFLQLTHPEDRAMVRKHIDACMRERRRIDIEYRIVHADGSLRWVWERGVG 107 + L ++ I + HPED +R + CM ++R D+E R V DGS+ W+ Sbjct: 389 EECLAMDDFIG--TIAHPEDEPRIRAELARCMDGQKREDVELRCVRKDGSVFWLALSCAP 446 Query: 108 LYNAAGKVEAMEGFLQDVTERKEAAHALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNP 167 +++A G+ +D+T+RK+ EAE R ++ ++ EG+ T + + VN Sbjct: 447 IHDAQGRFTGFRTSSRDITDRKQI-----EAELRISAVAFDSREGMLITDANSKILRVNK 501 Query: 168 ALARIYGYHSPEDLIVG-----LRDITHQLYVEPERRAEFMRLMEREGSVSNFESRVYRR 222 A I GY + E IVG LR H E R R + +G + + R+ Sbjct: 502 AFTEITGYSAEE--IVGKHPNLLRSDRHGPAFFQEMRESIRRFGKWQGEIWD-----RRK 554 Query: 223 DGDII--WISENARAVYDDSGKLVCYEGTVEAITERKLYEAEMRHQATHDALTGLPNRNM 280 +G++ W++ AV + ++ Y T I++RKL E +R A DALT LPNR + Sbjct: 555 NGEVYPEWLT--ISAVKNKDQQVTHYVSTHHDISDRKLAEERIRELAFFDALTRLPNRTL 612 Query: 281 LHEHLQRAIQVARQKGGLTAVVFVDLDQFKFINDSLGHQVGDELLKAVAQRLQACLRDTD 340 L + L++AI ++ + A++F+DLD FK +ND++GH+ GD LLK VAQRL A +R+ D Sbjct: 613 LLDRLKQAIALSLRTKTCAALLFIDLDHFKTLNDTIGHEKGDTLLKQVAQRLLANVREND 672 Query: 341 MVARQGGDEFVLVLQN----QTGGELGIAEVMQRILAAVARPWQTGDRELQITASIGVSR 396 VAR GGDEFV+VL+ Q + ++IL A+ +Q D E + TASIG + Sbjct: 673 TVARVGGDEFVVVLEGLNELQQEAATQTRAIGEKILTALGSTYQLDDVEYRTTASIGATV 732 Query: 397 YPADGKDVETLLKQADSAMYRAKEQGRNNFQFFAPWMDTQVSNRLEMLINLRRALDHDEF 456 + V+ L+KQAD AMY+AKE GRN +FF P M T V R + LR+A+D ++ Sbjct: 733 FLGRAASVDELMKQADLAMYKAKETGRNALRFFDPAMQTAVLERATLEAGLRKAIDGNQL 792 Query: 457 KLYYQPKLSLKDGRVIGAEALIRWQSPEQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACH 516 L+YQP++ ++ GRV GAE L+RWQ P++GMVPP FIP AEE GLI+ +G WVL TAC Sbjct: 793 LLHYQPQV-VEGGRVTGAEVLVRWQHPQRGMVPPGDFIPLAEETGLILSLGNWVLETACR 851 Query: 517 QNKRWQ-QAGLPPIPVAVNLSPRQLNQSLPDFVSGVL---AQSGLDAACLELEITENVVM 572 Q W Q + + +AVN+S QL + PDFV VL ++G + L+LE+TE++++ Sbjct: 852 QLATWAAQPQMADLSIAVNVSAPQLRE--PDFVQTVLDTIERTGANPRRLKLELTESLLV 909 Query: 573 KDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDKSFVRDIARDADSA 632 + E + + ALK G++ S+DDFGTGYSSLSYL+R P++ LKID+SFVRD+ D + A Sbjct: 910 DNVEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLKRLPLNQLKIDQSFVRDVLIDPNDA 969 Query: 633 AIVKAVISLAHILNLRVIAEGVEDEQQHAFLKENACDEVQGYYFGKPMAVEDF 685 +I K +++LA L L VIAEGVE + Q +FL + C QGY+F +P+ VE F Sbjct: 970 SIAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGCHAYQGYFFCRPVTVEAF 1022 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 404 bits (1038), Expect = e-113, Method: Compositional matrix adjust. Identities = 233/574 (40%), Positives = 335/574 (58%), Gaps = 20/574 (3%) Query: 124 DVTERKEAAHALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLIV 183 D +R A ++ + I E V P G + N +++GY + E + Sbjct: 106 DTEQRVRALDQIEAKLEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSAME--AI 163 Query: 184 GLRDITHQLYVEPERRAEFMRLMEREGSVSNFESRVYRRDGDIIWISENARAVYDDSGKL 243 G R++ E E + +E G E R ++ G++ W S + + D +G Sbjct: 164 G-RNVLFLYENEDEEDSLHDLFLEHGGR--EMEVRRRKKSGEVFWASLSLSVMRDQNGHP 220 Query: 244 VCYEGTVEAITERKLYEAEMRHQATHDALTGLPNRNMLHEHLQRAIQVARQKGGLTAVVF 303 + G + ITERK E + H A +DALTGLPNR +L + +A++ AR + V+F Sbjct: 221 IGLIGYLNDITERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMF 280 Query: 304 VDLDQFKFINDSLGHQVGDELLKAVAQRLQACLRDTDMVARQGGDEFVLVLQNQTGGELG 363 +DL++FK IND+LGH GD LL VA RL+ LR+ D+VAR G DEF + L G Sbjct: 281 IDLNRFKPINDTLGHVAGDMLLVEVAMRLRRALREQDVVARLGADEFAVAL-------FG 333 Query: 364 I-----AEVMQRILAAVARPWQTGDRELQITASIGVSRYPADGKDVETLLKQADSAMYRA 418 I A V Q++LA P+ EL++ ASIGVS YP + D ETLL+ AD AMYRA Sbjct: 334 IDKDSPAYVAQKLLALFDDPFFIDGHELRVGASIGVSLYPENATDTETLLRLADIAMYRA 393 Query: 419 KEQGRNNFQFFAPWMDTQVSNRLEML---INLRRALDHDEFKLYYQPKLSLKDGRVIGAE 475 K+ G NN +A + + N L++L L A +++E LYYQPK+ ++ + GAE Sbjct: 394 KQGGENNEGGYACYSEEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAITGAE 453 Query: 476 ALIRWQSPEQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACHQNKRWQQAGLPPIPVAVNL 535 AL+RWQ PE+G++ P FIP AEE GLIV + +WVL C Q + W+ AGLPPI +A+N+ Sbjct: 454 ALVRWQHPERGLLLPGEFIPIAEETGLIVQLSDWVLDAVCRQARAWKDAGLPPIRIALNV 513 Query: 536 SPRQLNQSLPDFVSGVLAQSGLDAACLELEITENVVMKDAEKSVATLHALKRLGLQISVD 595 + R+ +SLPD V L + L LELEITE+++M ++ ++ + + LG+ +S+D Sbjct: 514 TAREFTRSLPDRVGAALERHMLSGEWLELEITESMLMHSTDRVISIMEKICGLGITVSLD 573 Query: 596 DFGTGYSSLSYLRRFPVDALKIDKSFVRDIARDADSAAIVKAVISLAHILNLRVIAEGVE 655 DFGTGYSSLSYL+RFP++ LKID+SF I D + AI A+I +A L +VIAEGVE Sbjct: 574 DFGTGYSSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVE 633 Query: 656 DEQQHAFLKENACDEVQGYYFGKPMAVEDFTAWM 689 DE+Q FL+E C+E+QGY F +P+ DF A + Sbjct: 634 DEKQFNFLREAGCNELQGYIFSRPVPAPDFHAML 667 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 391 bits (1004), Expect = e-109, Method: Compositional matrix adjust. Identities = 194/450 (43%), Positives = 296/450 (65%), Gaps = 3/450 (0%) Query: 252 AITERKLYEAEMRHQATHDALTGLPNRNMLHEHLQRAIQVARQKGGLTAVVFVDLDQFKF 311 AI R+ E +RH A +D LTGLPNR + +H+ AI A + G+ AV+ +DLD+FK Sbjct: 656 AIESRRADE-RIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKN 714 Query: 312 INDSLGHQVGDELLKAVAQRLQACLRDTDMVARQGGDEFVLVLQNQTGGELGIAEVMQRI 371 IND+ GH+ G+ +L+ +AQR + LR+ D++AR GGDEF+ VL + + + E+ Q++ Sbjct: 715 INDTFGHETGEAVLQEIAQRFRNSLRELDILARVGGDEFI-VLVDDFDDPVQLGEIAQKL 773 Query: 372 LAAVARPWQTGDRELQITASIGVSRYPADGKDVETLLKQADSAMYRAKEQGRNNFQFFAP 431 L +P+ +E ++ SIG++ YP DG + +TL+K AD AMYRAK G+++++FF+ Sbjct: 774 LTEARKPFIIDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSD 833 Query: 432 WMDTQVSNRLEMLINLRRALDHDEFKLYYQPKLSLKDGRVIGAEALIRWQSPEQGMVPPD 491 ++T R+ + LRRA++ EF ++YQPK+ L +GR++GAEAL+RWQ P +G++PP Sbjct: 834 EINTNTVERIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPG 893 Query: 492 RFIPFAEEAGLIVPIGEWVLRTACHQNKRWQQAGLPPIPVAVNLSPRQL-NQSLPDFVSG 550 FI AEE LI IG VL AC +Q + +++NL+ Q ++ L D + Sbjct: 894 EFITLAEETRLIDQIGLLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIKE 953 Query: 551 VLAQSGLDAACLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRF 610 V+ + G +CLE EITE++VM + E+++A + ++ G IS+DDFGTGYSSL+YL+RF Sbjct: 954 VVDRHGTPPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRF 1013 Query: 611 PVDALKIDKSFVRDIARDADSAAIVKAVISLAHILNLRVIAEGVEDEQQHAFLKENACDE 670 PV+ LKIDKSF+ DI + +S+AIV+A+I+++H LNL+V+ EGVE E+Q L+E DE Sbjct: 1014 PVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDE 1073 Query: 671 VQGYYFGKPMAVEDFTAWMSRQGPTAQVLS 700 QGYYF KP+ F ++ Q Q L+ Sbjct: 1074 YQGYYFSKPVPYAQFVQLLAGQQDRPQGLA 1103 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,190,953 Number of extensions: 91620 Number of successful extensions: 424 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 332 Number of HSP's successfully gapped: 48 Length of query: 702 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 610 Effective length of database: 1,184,250 Effective search space: 722392500 Effective search space used: 722392500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)